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Citations for Millipore Sigma :
1751 - 1800 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Sera were incubated for one hour at room temperature followed by three washes with PBST before incubating with an anti-mouse IgG secondary antibody conjugated to alkaline phosphatase (Sigma) used as a 1:5000 dilution for one hour ...
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bioRxiv - Immunology 2021Quote: ... plates were again washed thrice followed by incubation with 1:5,000 dilution of horse radish peroxidase (HRP) conjugated anti-guinea pig IgG secondary antibody (Sigma-Aldrich) or 1:2,000 dilution of HRP conjugated anti-mouse IgG secondary antibody (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... the blot was washed with 1×TBS-T solution (4×5min) and incubated with anti-Mouse IgG-HRP secondary antibody solution (Sigma, 1:10,000 dilution in 1×TBS-T with 0.2% Blocking Reagent [Roche] ...
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bioRxiv - Developmental Biology 2020Quote: HRP-conjugated secondary antibodies for Western blotting: goat anti-mouse IgG (Cat# A 4416, Sigma-Aldrich, St. Louis, MO, USA) and goat anti-rabbit IgG (Cat# GtxRb-003-EHRPX ...
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bioRxiv - Plant Biology 2019Quote: ... four times and then placed for 1 h in a solution of goat antirabbit IgG (dilution 1:17500) conjugated to alkaline phosphatase (Sigma). The membrane was rinsed in TBS-T four times ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... TN) and the secondary antibody HRP-conjugated anti-goat IgG from rabbit was purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The plates were then washed again and incubated with horseradish peroxidase-conjugated rabbit anti-horse IgG (1:1000; Sigma, UK) for 3 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed then sequentially incubated with anti-SARS-CoV-2 CR3022 antibody (Yuan et al., 2020) (1 μg/mL) and a HRP-conjugated goat anti-human IgG (Sigma) in PBS supplemented with 0.1% (w/v ...
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Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threatsbioRxiv - Molecular Biology 2019Quote: ... Tubulin as loading control was detected with anti-tubulin (1:10000, MBL) and HRP-conjugated anti-rabbit IgG as the secondary antibody (1:10000, Sigma). For detecting the heperphosphorylation of Crt1-13MYC ...
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bioRxiv - Microbiology 2021Quote: ... An α-mouse IgG conjugated to horseradish peroxidase (HRP) was used as the secondary antibody at a dilution of 1:4,000 (Sigma-Aldrich). To assess cell wall fractionation a mouse α-protein A (Spa ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich) in PBST ...
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bioRxiv - Cell Biology 2021Quote: ... plates were washed 4 times with PBST and further incubated with 100 μL per well of goat anti-human IgG (Fc specific)-Peroxidase antibody (1: 5000 dilution, Sigma) for 1 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... The cleared extract was adjusted to 0.5% Triton X100 and 0.1% Tween 20 and added to magnetic beads prepared from 400 microlitres of an IgG (rabbit serum, I5006-100MG, Sigma)-coupled magnetic beads slurry (1 mm BcMag ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Microbiology 2020Quote: rAkata EBV-infected cells was reactivated at 1×106 cells/mL with a goat polyclonal anti-human IgG Fc-specific antibody (Sigma) for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 2h incubation with Alexa Fluor-488 goat anti-rabbit IgG secondary antibody (1:500, A11008, Merck Millipore, MA). Slices were transferred to glass slides and covered with Fluoromount (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... the protein transferred membranes were blocked with 5% dry milk in PBST (PBS with 0.05% tween-20) overnight at 4°C and probed with goat anti-human IgG-HRP (Sigma-Aldrich, St ...
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bioRxiv - Immunology 2020Quote: ... Viral plaques were stained with anti-LCMV nucleoprotein (anti-LCMV NP, clone VL4) and HRP conjugated anti-rat IgG (Millipore) and visualized with O-phenylenediamine (OPD ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... Detection was carried out using anti-mouse IgG-HRP antibody (GE; 1:5000 in PBS + 2% BSA) followed by addition of TMB substrate (Sigma). Reaction was stopped by adding 0.5 M H2SO4 ...
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bioRxiv - Immunology 2022Quote: ... The membrane was then washed three times and incubated for one hour with HRP-conjugated goat anti-rabbit IgG (Sigma) at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Secondary antibody HRP conjugated-rabbit anti-goat IgG (cat#: AD106P) and primary anti-MPO antibody (cat#: AF3667) were purchased from Sigma and R&D systems ...
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bioRxiv - Molecular Biology 2023Quote: ... The blot was incubated for 1 h with a 1:5000 dilution of the secondary antibody (anti-rabbit IgG horseradish peroxidise-conjugated antibody, Sigma) in antibody buffer and then washed three-times for 15 min in TTBS ...
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bioRxiv - Microbiology 2022Quote: ... RBD or spike-bound serum antibodies were detected with horseradish peroxidase conjugated goat anti-mouse IgG (1:500 dilution, Milipore Sigma) incubating for 2 h at 25°C ...
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bioRxiv - Neuroscience 2022Quote: ... Washing steps were performed using PBS-Tween buffer followed by horseradish peroxidase-conjugated goat anti-mouse IgG antibody (Sigma-Aldrich) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... the microarrays were overlaid with the VP1 proteins for 90 minutes as protein-antibody complexes that were prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... protein (1:50 dilution) (BEI Resources, NR-619) and the secondary anti-mouse IgG antibody conjugated with FITC (1:100 dilution, Sigma). The cell nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... and 3% BSA followed by overnight incubation at room temperature with rabbit anti-KCC2 IgG (1:1000 dilution; #07-432, Merck-Millipore) (Yassin et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The pre-cleared chromatin was then incubated overnight at 4°C with 10 µL of either rabbit IgG (1 mg/mL, IGBMC facility) or rabbit anti-CTCF (07-729, Millipore). Immunoprecipitated chromatin was then adsorbed to 100 µL prepared protein A/protein G-Dynabeads for 3.5 h at 4°C on a rotating wheel ...
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bioRxiv - Biophysics 2023Quote: ... Anti-α-tubulin and anti-Nup153 antibodies were respectively revealed by ATTO647N goat anti-mouse IgG (1:400, Sigma-Aldrich) and STAR580 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... France) and secondary (anti-mouse IgG peroxidase-conjugated, 1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) antibodies were added at RT for 1 h ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant solution was removed and the cells were treated with a secondary antibody (the FITC-conjugated anti-mouse IgG (Sigma)) for one hour at 37°C in the darkness ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was washed 3X with 1x TBST for 5 min each followed by incubation with anti-rat IgG secondary antibody conjugated with horseradish peroxidase (HRP) (Sigma) (1:2000 dilution ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted 1:10000 in Intercept Blocking Buffer for 1.5 hour at room temperature or HRP-conjugate antibodies goat anti-rabbit IgG (A0545, Sigma-Aldrich) and anti-mouse IgG (A4416 ...
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bioRxiv - Neuroscience 2023Quote: ... We then washed the slices 5 times in PB 0.1 M and incubated them with primary antibody rabbit IgG antiGABA (Sigma A2052) diluted in PB 0.1 M 1:1000 with 1% NGS overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 100uL of Pierce Protein A/G Magnetic Beads were coupled to 10 µg of antibody or control rabbit IgG antibody (Sigma). DNA was incubated with antibody-coupled beads and after uncoupling ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed three times with PBS and stained with anti-rabbit IgG antibody conjugated to Alexa488 (Sigma- Aldrich, SAB4600045) and anti-mouse IgG antibody conjugated to Alexa647 (Thermo Fischer Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The secondary antibodies used for IB were goat anti-rabbit (Cat # AP307P) and goat anti-mouse (Cat # AP308P) IgG HRP-conjugated antibodies (Merck Millipore).
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bioRxiv - Biochemistry 2022Quote: Two commercially available mAbs (immunoglobulin or IgG, type 1) were used for the fragmentation experiments: Sigma mAb standard (SiLuLite, IgG1 lambda, CHO, Sigma) and NIST mAb standard (HzIgG1 kappa ...
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bioRxiv - Genetics 2022Quote: ... for CLK and BMAL1 expression then the secondary antibody of horseradish peroxidase-conjugated either anti-mouse or anti-rabbit IgG antibody (Sigma). Chemiluminescence detection was performed by using ECL Western Blotting detection Reagent (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... the microarray was overlaid with the VP1 protein for 90 minutes as a protein-antibody complex that was prepared by preincubating VP1 with mouse monoclonal anti-polyhistidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 4:2:1 (by weight ...
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bioRxiv - Immunology 2023Quote: ... followed by streptavidin-Cyanine-3 (1:500; Amersham Bioscience) or by goat anti-human IgG coupled to FITC (1:100, Sigma), as appropriate ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then washed and incubated with H3K27me3 antibody (1:200 dilution, Cell signalling 36B11#9733, control with IgG rabbit Sigma) for 2h30 at 4 °C on a rotating wheel and then washed twice ...
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bioRxiv - Microbiology 2023Quote: ... or anti-Ace-2 antibody (ab15348) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Cell Biology 2024Quote: ... Following secondary antibodies at the indicated dilution were used for IF: Anti-rabbit IgG (Fc) TRITC (Sigma #SAB3700846, 1:500), Anti-mouse IgG (Fc ...
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bioRxiv - Microbiology 2023Quote: ... incubated for 1 h at room temperature with an anti-mouse IgG secondary antibody conjugated with peroxidase (Sigma Aldrich, A9044) diluted 1:10,000 in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... TMT-labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (P3300, Sigma) overnight at 4℃ ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 3x with 200μLs of PBS-T per well and anti-mouse IgG HRP-conjugated secondary (Sigma, #AP308P) diluted at 1:3000 in blocking solution was added to each well and incubated for 1 hour at room temperature ...