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Citations for Millipore Sigma :
1601 - 1650 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and SARS2-71 [40] anti-S protein antibodies and HRP-conjugated goat anti-mouse IgG (Sigma Cat # A8924) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Cancer Biology 2021Quote: ChIP-grade mouse anti-PLK1 (Cat. # SAB1404220) and mouse IgG (Cat. #sc2025) antibodies were obtained from Sigma Aldrich. Anti-FLAG (Cat ...
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bioRxiv - Immunology 2020Quote: ... eluted with IgG elution buffer and concentrated using 10-kDa Amicon ultra-15 centrifugal filter units (EMD Millipore).
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bioRxiv - Cell Biology 2021Quote: ... the secondary antibody used was goat anti-rabbit immunoglobulin G (IgG) conjugated with horseradish peroxidase (Sigma, 12-348); and protein bands were visualised via chemiluminescence using an ECL Plus Western blotting detection kit (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... phospho-STAT2 (Tyr689) rabbit polyclonal IgG antibody antibody with epitope matching at phosphorylated Tyr689 (EMD Millipore #07-224), STAT2 (D9J7L ...
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bioRxiv - Immunology 2022Quote: ... the sections were washed with PBS/0.05% Tween-20 and incubated in anti-rabbit IgG-AP (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP (AP182P, Merck Millipore) or donkey anti-mouse IgG HRP (AP192P ...
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bioRxiv - Pathology 2022Quote: ... respectively an anti-rat IgG conjugated to alkaline phosphatase (1:500) (Sigma-Aldrich®, Merck KGaA, Darmstadt, Germany), for 60 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Antibodies were detected with anti-rabbit or anti-mouse IgG secondary antibodies conjugated to HRP (1:80,000; Sigma). Antigen-antibody complexes were visualized using enhanced chemiluminescence substrate (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... then incubated for two hours at room temperature with horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma A6917) diluted 1:1800 in PBS ...
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bioRxiv - Immunology 2023Quote: ... AP-labeled goat anti-human IgG (γ-chain specific; Sigma-Aldrich, Vienna, Austria; 1:50,000 in TBS BSA) and AP-labeled goat anti-human IgA (α-chain specific ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with HRP-conjugated goat anti-rabbit IgG (1:20,000) and developed using 0.2 mg/mL TMB (Sigma), 0.015% H2O2 in 100 mM sodium acetate ...
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bioRxiv - Neuroscience 2022Quote: ... goat anti-rabbit IgG (H+L) Highly Cross-Adsorbed CF®594 (Sigma-Aldrich, Cat#SAB4600110, 1:1000), goat anti-mouse IgG Abberior STAR 635P(Abberior Cat# ST635P-1001-500UG ...
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bioRxiv - Microbiology 2023Quote: ... followed by secondary antibodies goat anti-rabbit IgG horseradish peroxidase (1:10,000 dilution, Sigma-Aldrich, Cat. #12-348) or goat anti-mouse IgG horseradish peroxidase (1:10,000 dilution ...
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bioRxiv - Biophysics 2023Quote: ... Elution fractions containing highest concentrations of IgG were pooled and concentrated using Amicon Ultra-15 centrifugal filters (Millipore). The final concentration of purified mouse IgG was determined with a Bradford assay ...
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bioRxiv - Biochemistry 2023Quote: ... All assays were conducted using FP buffer [SEC buffer plus 0.1 mg mL-1 bovine IgG (Sigma-Aldrich) and 0.5 mM Thesit (Sigma-Aldrich)] Binding affinities were determined using SOLVER ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with 10 µg/ml of capture anti-human IgG (Sigma Aldrich, I2136) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with a biotinylated donkey antibody to rabbit IgG (10 μg/ml; AP182B, Merck Millipore) and then with Alexa488-conjugated streptavidin (2.5 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS-Tween20 (0.1%) and incubated with secondary-HRP conjugated antibodies (1:10.000; Anti Rabbit IgG HRPO, Millipore BI2407 ...
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bioRxiv - Biochemistry 2023Quote: ... The 139H2 membranes were washed again with TSMT and incubated with α-mouse IgG secondary antibody (A2304, Sigma) diluted 1:8000 in TSMT with 1% BSA for 1 h at RT ...
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bioRxiv - Synthetic Biology 2023Quote: ... and incubated for 2 h in 1:20,000 horseradish peroxidase (HRP)-conjugated goat α-mouse IgG (Sigma-Aldrich)/1% BSA/TBS ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and HRP-conjugated Anti-Rabbit IgG Secondary Antibody) and R&D Systems/Minneapolis/MN (14-3-3-Sigma polyclonal goat IgG and HRP-conjugated Anti-Goat IgG Secondary Antibody) ...
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bioRxiv - Plant Biology 2024Quote: ... Commercial alkaline phosphatase (AP)-conjugated goat anti-rabbit (IgG) antibodies (Sigma-Aldrich, USA, diluted 1:5,000 in PBS) were added and incubated for 2 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: goat polyclonal IgG anti-Choline Acetyltransferase (ChaT, Millipore Sigma, AB144P, 1:500), rabbit polyclonal IgG anti-5-HT (serotonin ...
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bioRxiv - Microbiology 2024Quote: ... Bound antibodies were probed using 50 μl/well of 1:1,000 of anti-mouse IgG HRP (Sigma–Aldrich) and incubated for an hour at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Sections through the striatum were stained with a mouse monoclonal anti-TH IgG (1:1,000 dilution; Sigma-Aldrich), and then with a goat anti-mouse IgG conjugated with Alexa Fluor 488 (1:400 dilution ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed and 25 µL/well of alkaline phosphatase-conjugated goat anti-human IgG (Sigma-Aldrich, US) and IgA (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... Next day membranes were incubated with peroxidase conjugated anti-rabbit IgG raised in Goat (Sigma, 1:8000 dil.) and peroxidase conjugated anti-mouse IgG raised in Rabbit (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Horseradish peroxidase-conjugated goat anti-chicken IgY and horseradish peroxidase-conjugated rabbit anti-rat IgG (Sigma, St. Louis, MO) were used for western blotting at a dilution of 1:80,000 ...
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then incubated with rabbit anti-mouse IgG-FITC polyclonal antibody at 1:500 (F9137, Sigma, Milan, Italy) in blocking solution at RT for 2h and counterstained with 0.5μg/mL PI at RT for 5 min ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with peroxidase-coupled secondary IgG antibodies (anti-Rabbit–A0545 or anti-Mouse –A9044, Sigma Aldrich) for 2 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were incubated for 1 h at room temperature with horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma) at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... For all ChIP experiments a mock IP and an experimental IP were set up at the same time using 1,350 µl pre-cleared chromatin and 10 µg of normal mouse IgG (Millipore) and monoclonal mouse anti-c-Myc respectively ...
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bioRxiv - Plant Biology 2020Quote: ... at a 1:1000 dilution and the corresponding secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (Sigma) at a 1:10,000 dilution were used to analyze the linkage type of PhS3-RNase ubiquitination.
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bioRxiv - Immunology 2021Quote: ... Free active groups were blocked with 0.5M Tris (pH 8.0) (1 hour, room temperature) before opsonizing with rabbit anti-BSA IgG (Sigma, B1520). IgG-free BSA treatment and blocking prior to opsonization was omitted for beads and surfaces opsonized with human IgG (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Alkaline phosphatase-conjugated goat anti-rabbit IgG was employed as a secondary antibody (Sigma-AldrichR, St. Louis, MI, USA). The antigen–antibody complex was detected by the alkaline phosphatase reaction ...
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bioRxiv - Plant Biology 2021Quote: ... for detection of FLS2-BAK1 co-immunoprecipitation or anti-rabbit IgG (whole molecule)–HRP (A0545, Sigma, dilution 1:10000) for all other western blots.
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatants were pre-cleared twice by incubation with 28 μL 50% mouse IgG agarose slurry (Sigma #A0919) for 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The loading control antibody was probed with HRP-conjugated goat anti-mouse secondary IgG (SAB3701047, Sigma, St. Louis, MO) for 1 hr at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... followed by 60 min incubation with 1:1000 dilution of Cy3 anti-rabbit IgG antibody (0.1 µg/ml; Sigma). The cells were washed again with 1x PBST (S3 Table ...
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bioRxiv - Cancer Biology 2022Quote: ... were coated with 3 μg of specific antibody or control IgG by resuspending them in 60 μl PBS containing 0.02% Tween-20 (P1379, Sigma), and rotated for 45 min at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Developmental Biology 2020Quote: ... membrane was incubated for 1 h at room temperature with anti-rabbit IgG horseradish peroxidase (HRP) conjugate (Sigma-Aldrich) at a concentration of 1:1000 in 5% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... the cells were washed twice with PBS and incubated with anti-rabbit IgG-FITC antibody (1:50; Sigma-Aldrich) for 40 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1000µg of the soluble fraction were taken for IP using 2µg of YAP antibody (NB110-58358, Novus) or IgG control (#I5006, Sigma), and 25µl of magnetic beads ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by washing and staining with horseradish peroxidase-conjugated donkey anti-rabbit or anti-mouse IgG secondary antibodies (1:5,000 dilution; Merck Millipore), respectively ...
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bioRxiv - Epidemiology 2020Quote: ... An in-house polyclonal anti-flavivirus antibody (anti-DENV 1-4) and an anti-mouse IgG-FITC antibody (Sigma) were used ...
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bioRxiv - Plant Biology 2021Quote: ... For Co-IPs with MED10B-MEC-ProtA we used IgG Sepharose® 6 Fast Flow (Sigma, GE17-0969-01) beads and increased washing steps (1xIP buffer ...