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Citations for Millipore Sigma :
1801 - 1850 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... and labelled with the secondary antibody (diluted 1:50 in 0.1% BSA in PBS, Anti-Rat-IgG – Gold antibody; Sigma, USA) for 1 h at 37°C ...
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bioRxiv - Immunology 2024Quote: ... An equal volume of IgM antibody at a concentration of 150-200µg/mL was mixed with equal volume of human IgG obtained from blood (Sigma-Aldrich) at a concentration of 1mg/mL (ratio ∼1:5 ...
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bioRxiv - Microbiology 2023Quote: ... The wells were washed as before and then probed with 100µl rabbit anti-chicken IgG conjugated to horseradish peroxidase (HRP) (Sigma Ltd.) diluted 1:3000 in ELISA diluent for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... HSF5-N2 and control IgG antibodies were crosslinked to 50 µl of protein A-Dynabeads (Thermo-Fisher) by DMP (Sigma). The antibody-crosslinked beads were added to the testis extracts prepared above ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes previously incubated with anti-RplA were incubated with the secondary antibody anti-rabbit IgG-HRP (#A8275, 1:10000, Sigma) for 1 h at room temperature and then washed three times with T-PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... the plate was washed 5 times with PBS and incubated for 1 hour with goat anti-human IgG (Fc specific) –peroxidase (#A0170, Sigma). Binding signals were developed and stopped by the addition of TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Cancer Biology 2024Quote: ... was used as the primary antibody and a goat polyclonal anti-rabbit IgG conjugated to horseradish peroxidase (Millipore, 12-348) for the secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... 10 ng recombinant IdeS was pre-incubated with sera dilutions as indicated at 37°C 1 h before addition of 100 ng human IgG (Sigma) in assay diluent (AD ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was washed in TBST and blocked again with BSA followed by incubation with anti-rat IgG conjugated with horseradish peroxidase (dilution 1:20000, cat. no. A5795, Sigma) in TBSTB for 1 hour at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and MreB or Spo0J were detected using respective in-house polyclonal antibodies and HRP-conjugated anti-rabbit IgG antibody (Sigma). Samples were developed using Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for immunoprecipitation: HSF1 (ADI-SPA-901, Enzo), HSF2 (Östling et al., 2007) and normal rabbit IgG (EMD Millipore). Crosslinks were reversed by incubating the samples at 65°C overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... fixed cells were permeabilized with PBS containing 0.2% Triton X-100 for 10 min and blocked with PBS containing 1% IgG-free bovine serum albumin (BSA) (Sigma, A2058) for 30 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... slices were washed three times in PBS for 5min and incubated with biotinylated anti-rabbit IgG (1:500 in PBS, Sigma-Aldrich) at room temperature (RT ...
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bioRxiv - Genomics 2022Quote: ... and 800ul of antibody buffer containing 1ul of primary antibodies (normal rabbit IgG, Santa Cruz Cat no sc-2027, H3K27me3 (Millipore, Catalogue number 07-449 ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... the cryosections were incubated with Alexa FluorTM 488 goat-anti-Rabbit IgG (1:1000) for 2 hours at room temperature and then the nuclei counterstained with Hoechst 33342 (Sigma-Aldrich). The cryosections were imaged using the A1+ Confocal Microscope System with NIS-Elements Advanced Research software (Nikon ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were probed with anti-gePABP (1:2000) primary antibodies (77) and horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibodies (Sigma-Aldrich) (1:50000) ...
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bioRxiv - Cell Biology 2022Quote: Affinity-purified rabbit anti-AQP5 IgG antibody targeted towards amino acid residues 249-265 of rat AQP5 (AB15858) and normal goat IgG antibody (NI02-100UG) were obtained from Millipore Sigma. Normal rabbit IgG antibodies were obtained from Cell Signaling Technology (2729 ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed six times and incubated with 100 μL/well horseradish peroxidase (HRP)-conjugated goat anti-human IgG (Fc-specific, Sigma Aldrich) diluted 1/5000 in PTA buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... The RBM proteins were identified with the primary rabbit anti-RBM10 or RBM5 antibody and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems).
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with PBS for 1 h at 37°C and then incubated with a FITC-conjugated rabbit anti-mouse IgG antibody (Sigma-Aldrich) at a 1:500 dilution ...
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bioRxiv - Plant Biology 2019Quote: ... 10 µL protein G Dynabeads® were washed twice in ChIP dilution buffer and incubated either with 2 µg of mouse IgG (Sigma), anti-H3K27me3 (Abcam ab6002 ...
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bioRxiv - Neuroscience 2019Quote: ... This was followed by a rinse with phosphate buffered saline and application of the diluted primary antibodies: whole molecule Anti-Rabbit IgG (1:200, goat, FITC antibody, Sigma-Aldrich) then secondary antibodies (Whole molecule ...
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bioRxiv - Molecular Biology 2019Quote: ... NF-kB (CST, #3033), PCM1 (CST, #5213) and GABARAP (CST, #26632) and NDP52 (. Secondary antibodies: anti-Mouse IgG–Peroxidase (Sigma, A5906), anti-Rabbit IgG–Peroxidase (Sigma ...
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bioRxiv - Physiology 2020Quote: ... containing 5% w/v non-fat dry milk followed by anti-rabbit IgG HRP-conjugated goat secondary antibody (A0545, Sigma-Aldrich). Signal was detected using the SuperSignal West Dura Extended Duration Substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% of pre-cleared chromatin was set aside for input and 5 µg Monoclonal ANTI-FLAG® M2 antibody (to capture FLAG-tagged dCas9) or IgG (Sigma) was added to the remaining volume and then incubated at 4°C on a nutator overnight ...
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bioRxiv - Biochemistry 2019Quote: ... It was followed with further washes and incubation at room temperature with TRITC conjugated anti-rabbit IgG (1:400) (Sigma, USA) for 1h ...
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bioRxiv - Developmental Biology 2019Quote: ... cell lysates were subjected to immunoprecipitation overnight using an anti-mouse HSF2 (Santa-Cruz) and a non relevant IgG (Sigma-aldrich) as a negative control that were pre-incubated 1h at RT with Protein G UltraLink Resin beads (53132 ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed four times with PBST and 100 μl/well of goat anti-human immunoglobulins-peroxidase IgG (FC specific; Sigma-Aldrich), IgM (μ-chain specific ...
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bioRxiv - Microbiology 2019Quote: ... For antigen-antibody detection membranes were incubated for 1h at room temperature with a 1:2500 dilution of anti-rabbit IgG (whole molecule)— alkaline phosphatase antibody produced in goat (Sigma A0545) in PBS pH7.4 ...
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bioRxiv - Cell Biology 2019Quote: ... The RBM proteins were examined with the primary antibodies and the following secondary anti-rabbit IgG conjugated with Horse radish peroxidase (A6154; Sigma-Aldrich) or conjugated with alkaline phasphatase (T2180;Applied Biosystems) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the infected cells were fixed with cold acetone and tested using CPV-2-specific canine antibodies and rabbit anti-dog IgG conjugated with fluorescein isothiocyanate (Sigma Aldrich). Viral titre ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Immunology 2019Quote: ... The microarray was then rinsed for 3 times with washing buffer and incubated with 200 μL of Horseradish peroxidase (HRP) conjugated Rabbit anti-Goat IgG (Sigma-Aldrich) diluted 1:20000 in Peroxidase Conjugate Stabilizer/Diluent (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... bound antibodies were detected by addition of a 1 in 5000 dilution of alkaline phosphatase (AP)-conjugated goat anti-mouse IgG (Sigma-Aldrich) for 1 hour at 21°C and addition of p-Nitrophenyl Phosphate ...
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bioRxiv - Neuroscience 2021Quote: ... After three washes in PBS sections were incubated with secondary antibodies (anti-Rabbit IgG CF™ 568, 1:1000, Sigma Aldrich, SAB4600084 ...
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and NGS ...
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bioRxiv - Biochemistry 2021Quote: ... All wells were then incubated with 3,000-fold diluted goat anti-human IgG-alkaline phosphatase conjugate (Sigma-Aldrich, St. Louis, MO) for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... the membrane was washed with TBS-T and incubated for 90 min with anti-rabbit IgG antibody conjugated to alkaline phosphatase (Sigma Alrich), diluted 1:5.000 in TBS-T solution containing 0.75 % BSA ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Microbiology 2021Quote: ... Pre-cleared lysate was diluted to 500 μl with additional lysis buffer and incubated overnight with 40 μl Protein A agarose beads and 4 μg anti-FcRγ-subunit IgG (Millipore Sigma) at 4°C on a Labquake Rotator ...
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bioRxiv - Physiology 2021Quote: ... Sections were subsequently incubated with the following primary antibodies for 1 h at room temperature: polyclonal rabbit anti-laminin IgG (L9393, 1:700; Sigma-Aldrich), monoclonal mouse anti-MHCI IgG2b (BA-F8 ...
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bioRxiv - Genomics 2020Quote: ... Sheared chromatin was diluted using 3X volume of IP dilution buffer and pre-cleared for at least 4hr at 4°C using IgG beads (Sigma A0919). 1% of pre-cleared chromatin was kept as input ...
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bioRxiv - Cell Biology 2021Quote: ... or immunoprecipitating mAbs (mouse anti–cyclin B1 clone GNS3 or mouse anti–cyclin A clone E67.1; SC53230; Santa Cruz Biotechnology, Inc., or mouse IgG; Sigma-Aldrich) were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration ...
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bioRxiv - Biochemistry 2022Quote: ... in PBS for 1 hour at room temperature before probing with anti-Hs haemoglobin (rabbit IgG, 5 µg/mL, Sigma Aldrich), anti-HA (mouse monoclonal Clone HA-7 ...
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bioRxiv - Immunology 2022Quote: ... The virus:serum/IgG/mAb mix was added to TZM-bl cells which were supplemented prior with DEAE-dextran and Saquinavir (Sigma-Aldrich), as described above ...