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Citations for Millipore Sigma :
1701 - 1750 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... or 10μg of p53 antibody (Santa Cruz, DO1) (rabbit or mouse IgG was used as a negative control (Sigma, M7023)) for 4 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific) FITC (Sigma, F9512) and Anti-human IgG (Fc specific ...
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bioRxiv - Immunology 2021Quote: ... the membrane was incubated with anti-rabbit IgG antibody conjugated to horseradish peroxidase (Cat # A9044, Sigma Aldrich; 1:10,000 dilution) for 1 hr at RT and proteins visualized using Clarity ECL western blotting substrate (BioRad ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies were detected by incubation for 1 h with FITC-conjugated goat anti-mouse IgG (1:40, Sigma F8264) or Alexa Fluor 568-conjugated goat anti-mouse IgG (1:300 ...
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bioRxiv - Developmental Biology 2021Quote: Newborn pups were injected at P2 via the temporal vein with 0.0025mg/g of DLL4-neutralizing (blocking antibody (YW152F, Genentech (31)) or IgG from human serum (cat #I4506, Sigma Aldrich). The pups were sacrificed 48 h after injection at P4 ...
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bioRxiv - Molecular Biology 2021Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was incubated with an affinity-purified specific antibody (1:10,000) and goat peroxidase-conjugated antirabbit IgG (1:20,000, Sigma-Aldrich, USA), for 2 h at 25 ºC ...
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bioRxiv - Immunology 2020Quote: ... Foci were detected after the cells were incubated with a 1:5000 dilution of horseradish peroxidase-conjugated goat anti-guinea pig IgG (Sigma) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: Immunocytochemistry was carried out on fixed unpermeabilized cells with polyclonal antibodies anti-Xlenpp4 used at 1/200 or anti-mEnpp4 (CR65; see31) used at 1/400 and anti-rabbit IgG FITC (Sigma) at 1/80 ...
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bioRxiv - Immunology 2022Quote: ... we used the appropriate corresponding isotype control antibodies (mouse IgG1 K and mouse IgG2 B, both from Biolegend, goat IgG from SIGMA) and a drug carrier control.
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bioRxiv - Immunology 2022Quote: ... Plates were washed thrice with PBS-T followed by addition of peroxidase conjugated goat anti-mouse IgG diluted 15,000 fold and 20,000 fold (Sigma Aldrich, A4416) to RBD and N protein coated plates respectively ...
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bioRxiv - Genomics 2020Quote: ... Membranes were incubated with primary antibody in 5% milk/TBS-T (5% BSA/TBS-T for anti-RUNX1) overnight at 4°C and subsequently incubated with anti-Rabbit IgG peroxidase antibody (1/10,000, Sigma A6667) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2019Quote: ... and subsequently incubated overnight with the Notch ligand Delta-1 (courtesy of the Bernstein Lab, FHCRC) or human IgG (Sigma) to achieve oriented immobilization ...
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bioRxiv - Plant Biology 2020Quote: ... AtMPK4 and AtMPK6 was performed using anti-MAP kinase-antibody (1:20000 dilutions) as primary antibody and anti-mouse IgG (Sigma) protein (1:15000 dilutions ...
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bioRxiv - Biochemistry 2019Quote: ... anti-actin (A4700) and anti-tubulin antibody (T6199) and goat anti-human IgG–HRP antibodies were obtained from Sigma-Aldrich, Dorset ...
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bioRxiv - Biophysics 2019Quote: ... fab-fragment solution consisting of 100 μg/ml F(ab’)2 fragments (anti-mouse IgG [Fc specific] antibody developed in goat; Sigma) in PBS that bind unspecifically to the hydrophobic surface (incubation time 5 min) ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... the NC membranes were incubated with goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP; Sigma-Aldrich; dilution, 1:8000) and the color reaction was examined using ECL Kit (Amersham Pharmacia Biotech ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Biochemistry 2020Quote: ... membranes were incubated for 1 hour at room temperature with 1:10,000 diluted peroxidase-conjugated goat anti-rabbit IgG (Sigma, A6154). After 4 washes in PBS with 0.1% Tween 20 ...
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bioRxiv - Bioengineering 2020Quote: ... the plates were incubated with the mouse anti-HA antibody followed by goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich). The absorbance at 405 nm was read by the microplate reader (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... from 1:50 to 1:51200 in PBS containing 2% skimmed milk were added followed by ALP-conjugated anti-human IgG (A9544; Sigma) at 1:10,000 dilution ...
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bioRxiv - Physiology 2020Quote: The membrane was washed in TBST and incubated with a peroxidase-conjugated secondary antibody (goat, anti-mouse IgG, Sigma-Aldrich) diluted 1 ...
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bioRxiv - Plant Biology 2021Quote: ... LM19, LM2, JIM8 and JIM13 (PlantProbes, Leeds, UK) were used as primary antibodies and FITC–conjugated anti-rat IgG (Sigma) as secondary antibody in all cases ...
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bioRxiv - Biochemistry 2021Quote: ... or 680LT-conjugated anti-rabbit IgG (1:5000, Li-COR) and IRDye 800CW (Li-COR) labeled HPA (Helix pomatia lectin, Sigma) or PNA (peanut agglutinin ...
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bioRxiv - Cell Biology 2021Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... Serial dilutions of mouse sera were tested for binding to the biotinylated proteins and the bound antibodies detected using HRP-conjugated anti-mouse IgG A4416 (Sigma) and TMB substrate (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Plates were washed two times and horseradish peroxidase (HRP)-conjugated goat anti-human IgG antibody (Sigma Aldrich, St. Louis, MO) was used at a 1:5,000 dilution and incubated at room temperature for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... Immunoblot of phosphorylated MAPKs was performed using anti-phospho p44/42 MAPK antibody as the primary antibody and peroxidase-conjugated goat anti-rabbit IgG (Sigma) as the secondary antibody.
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bioRxiv - Microbiology 2021Quote: ... We just changed the secondary antibody and for this step horseradish peroxidase (HRP)-conjugated rabbit anti-bovine IgG antibody (Sigma) was used ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Systems Biology 2020Quote: ... TMT labeled samples were incubated in IP buffer consisting of 1% Nonidet P-40 with protein G agarose beads conjugated to 24 μg of 4G10 V312 IgG and 6 μg of PT-66 (Sigma) overnight at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... the plate was incubated with 50 µl of goat anti-rabbit IgG conjugated to alkaline phosphatase diluted 1:2000 (A3687, Sigma) in PBS for 1 h at RT ...
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bioRxiv - Microbiology 2019Quote: ... the slides were incubated for 2 hours at room temperature with 1 µg/mL Cy3-conjugated goat anti-human IgG (H+L) (Sigma) and washed again ...
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bioRxiv - Biophysics 2020Quote: ... these were washed three times with PBS and incubated for 2h with the anti-mouse IgG (whole molecule)-FITC secondary antibody (Sigma). Myonemes ...
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bioRxiv - Microbiology 2022Quote: ... These wells were washed five times using 1× PBST and followed by addition 50 μl/well of anti-human IgG horseradish peroxidase (HRP) (Sigma) diluted in Stabilzyme Noble (Surmodics) ...
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bioRxiv - Microbiology 2022Quote: ... cells were then incubated in a 1:5000 dilution of a horseradish peroxidase (HRP) conjugated goat anti-mouse IgG (Sigma). Titration of RUBV was performed similarly but with a polyclonal anti-RUBV goat IgG at 1:4000 (LifeSpan BioSciences ...
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bioRxiv - Microbiology 2022Quote: ... Membrane was washed three times with 1 X TBST and incubated for 1 h with 1:10,000 diluted secondary antibody (Anti-Rabbit IgG peroxidase, Sigma-Aldrich) at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The immunoreactivity was detected using the avidin-biotin detection method (biotinylated donkey-anti rabbit IgG (Sigma-Aldrich, Søborg, Denmark, #SAB3700966); avidin-biotin-peroxidase complex (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was washed with PBS-Tween 20 0.05% and incubated with peroxidase-conjugated anti-human IgG antibody (Sigma, USA) at a dilution of 1 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were probed with 1 in 10,000 dilution of human anti-SIC followed by 1 in 50,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich). To directly compare detection of SIC fragments by purified human and rabbit anti-SIC antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... up to 100 μg of carrier-free IgG antibody was subjected to buffer exchange by washing with R-buffer using a 50 kDa Amicon filter (Millipore) that was pre-soaked with R-buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then transferred to fresh 0.3% PBS-Triton and incubated overnight at 4°C with appropriate primary antibodies (1:200-500 dilution of goat anti-ChAT IgG, Millipore, AB114P ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in the dark in Alexa 555-conjugated or Alexa 488-conjugated goat anti-rabbit-IgG (1:300; Millipore Sigma) for 2 h following primary antibody incubation ...
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Immunology 2022Quote: ... and spike protein-bound antibodies were detected using an Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich, #A2429) at a 1:4000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: Immunoprecipitation was performed using SRF or MKL1 antibody or control rabbit IgG with Magnetic Protein A/G beads (Magna ChIP A/G kit, Millipore), as described previously (Chatterjee et al. ...
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bioRxiv - Neuroscience 2019Quote: ... and or Alexa Flour 488 anti-mouse IgG (1:800, A11001, Life) at room temperature for 1h and mounted with DAPI (F6057, Sigma). The sections were observed using fluorescence microscope (Olympus BX35 ...
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bioRxiv - Cancer Biology 2019Quote: ... Slides were then incubated with secondary antibodies for 30 minutes at a dilution of 1:15,000 (Anti-Rabbit IgG 488 (Sigma SAB4600234) and Anti-Mouse IgG 555 (SAB4600068)) ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were immunoprecipitated with 2 μg of IgG (negative control) or Smad3 antibody in coimmunoprecipitation reagents (17-195, Millipore). Semi-quantitative and quantitative PCR were performed to amplify α-SMA or Col1A promoter regions containing SBE.
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bioRxiv - Molecular Biology 2020Quote: ... The secondary antibodies used in this study were as follows: anti-mouse IgG whole molecule-Peroxidase (1:80,000; catalog no. A9044, Sigma-Aldrich) and anti-rabbit IgG whole molecule-Peroxidase (1:80,000 ...