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Citations for Millipore Sigma :
1501 - 1550 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... we used a 1:50,000 dilution of the anti-rabbit IgG-Peroxidase polyclonal antibody (Sigma-Aldrich, A0545). Signal detection was done using SuperSignal West FemtoMaximum Sensitivity Substrate (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... and SARS2-71 62 anti-S antibodies and HRP-conjugated goat anti-mouse IgG (Sigma, 12-349) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Microbiology 2021Quote: ... The following secondary antibodies were used in this study: goat anti-rabbit IgG-AP conjugate (Sigma Aldrich) (dilution 1:6,000 in 5% w/v skimmed milk/TBS-T) ...
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bioRxiv - Bioengineering 2019Quote: ... and then incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG antibodies (A0545, 1/2000; Sigma-Aldrich) for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... which in turn was detected using 1:5000 goat anti-mouse IgG alkaline phosphatase conjugate (Sigma, A3562).
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected by the following secondaries-1:10,000 goat anti-rabbit IgG HRP (Sigma Aldrich); 1:5000 donkey anti-chicken IgG HRP (Sigma Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... The plate was further washed three times and 50µL of anti-mouse IgG-HRP (A3673, Sigma-Aldrich) or anti-mouse IgM-HRP (A8786 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with a secondary Ab against IgG conjugated to fluorescein isothiocyanate (FITC) (Sigma-Aldrich) in PBST buffer for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The membranes were subsequently incubated with peroxidase-conjugated secondary antibodies (anti-mouse IgG, HRP conjugate, EMD Millipore, AP160P ...
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bioRxiv - Microbiology 2022Quote: ... Goat Anti-Rabbit IgG (whole molecule) Gold antibody was obtained from Sigma Aldrich (St. Louis, MO, USA).
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bioRxiv - Cell Biology 2022Quote: ... They were then overlaid with 10-15 μl of 1 mg/ml Human IgG (Millipore Sigma, I4506) mixed with either 20 μg/ml Goat anti-Mouse IgG Alexa Fluor 405 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... coupled to Xenopus TRAIP antibodies raised in rabbit and affinity purified or nonspecific rabbit IgG (I5006, Sigma). The TRAIP antibodies were coupled at 600 μg per 1 ml of beads ...
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bioRxiv - Immunology 2020Quote: ... Membranes were then probed with horse radish peroxidase (HRP) conjugated goat anti-rabbit IgG (Sigma-Aldrich, Germany) (diluted 1:10000 in 0.5% w/v skimmed milk in 1x PBS containing 0.05% BSA and Tween 20 (0.1% w/v ...
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bioRxiv - Microbiology 2020Quote: IgG from rabbit sera was affinity-purified with equal parts of Protein G Sepharose (Sigma-Aldrich, P3296) and 150 ml Protein A Sepharose (GE ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG (rabbit, A9169, 1:10000, Sigma) or anti-mouse IgG (mouse ...
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bioRxiv - Microbiology 2020Quote: ... and binding detected using 1 in 25,000 dilution of HRP-conjugated goat anti-human IgG (Sigma-Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Immunology 2022Quote: ... An anti-hamster IgG (H+L)-Alkaline Phosphatase antibody produced in goat (Sigma-Aldrich, St Louis, MO) diluted to 1:1000 in 3% BAS-PBS was added at 60 µl/well for 1 hour at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The diluted IgGs were then mixed with complement-preserved guinea pig serum (Sigma-Aldrich; final concentration 5%). Note that this concentration of guinea pig sera serum has been verified to not kill spirochetes in the absence of CspZ-YA antibodies (data not shown) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... anti-OmpF and anti-RNAP antibodies was anti-rabbit IgG coupled to HRP (A1949, Sigma-Aldrich, Germany). Bands were visualized with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were incubated in anti-GFP mouse IgG monoclonal primary antibody (Sigma, 11814460001; 1:1000-1:2000), anti-GST mouse IgG monoclonal primary antibody (Santa Cruz ...
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bioRxiv - Cancer Biology 2020Quote: ... The following HRP-linked secondary antibodies were used: anti-mouse IgG (NA931-1ML, Sigma-Aldrich, 1:10,000) and anti-rabbit IgG (NA934-1ML ...
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bioRxiv - Plant Biology 2021Quote: ... the proteins underwent binding with secondary antibodies diluted 5000-fold in PBS (anti-rabbit IgG; Sigma-Aldrich). Protein bands were visualized by staining with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... and SARS2-71 anti-S antibodies (33) and HRP-conjugated goat anti-mouse IgG (Sigma 12-349) in PBS supplemented with 0.1% saponin and 0.1% bovine serum albumin ...
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bioRxiv - Immunology 2021Quote: ... bound antibodies were detected by addition of alkaline phosphatase (AP)-conjugated goat anti-mouse IgG (Sigma-Aldrich) or anti-mouse IgM (Abcam ...
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... then incubated for 2 hours with secondary antibody - horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma, UK) diluted 1:1,500 with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... coupled to Xenopus Faf1 antibodies raised in rabbit and affinity purified or nonspecific rabbit IgG (15006, Sigma). The Faf1 antibodies were coupled at 600 μg per 1 ml of beads ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with primary mouse IgG anti-coronavirus group antibody MAB9013 (Millipore Sigma; diluted 1:500) for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Detection was performed with peroxidase-conjugated anti-mouse or anti-rabbit IgG (Sigma, St. Louis, MO, USA), and signal was developed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000, Sigma-Aldrich, cat.no. A2554).
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bioRxiv - Immunology 2020Quote: ... This was followed by detection with a goat polyclonal anti-mouse IgG HRP-conjugate (1/1000; Millipore). For all ELISAs ...
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bioRxiv - Immunology 2020Quote: ... IgGs were buffer exchanged into PBS by centrifugation with 30 kDa MWCO membrane centrifugal filter units (Millipore). Fabs were generated by papain digestion using crystallized papain (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... An HRP-conjugated goat anti-mouse IgG or an HRP-conjugated goat anti-mouse IgA (Sigma, USA) was used as the secondary antibody.
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bioRxiv - Immunology 2020Quote: ... intracellular staining was performed by 5 min pre-incubation with rat IgG in 0.5% saponin (Sigma-Aldrich) and addition of anti-CD154 antibody and anti-cytokine antibodies for another 30 min in 0.5% saponin at RT.
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bioRxiv - Microbiology 2021Quote: ... Glenn Bjork) at a dilution of 1:10,000 and a secondary anti-rabbit IgG antibody (Sigma-Aldrich) at a dilution of 1:160,000 ...
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bioRxiv - Microbiology 2020Quote: Secondary antibodies used for western blot included Goat anti–rabbit IgG-HRP (Sigma Aldrich A6154, 1:2000), Goat anti–mouse IgG-HRP (Sigma Aldrich A4416 ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were washed with TBS-T and incubated with goat anti-mouse IgG-peroxidase conjugate (Sigma DC02L) for 1 h at room temperature and washed again ...
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bioRxiv - Genetics 2022Quote: ... washed and then incubated for 1h with peroxidase conjugated anti-mouse IgG (Sigma Aldrich, A0168, 1:10,000) or anti-rabbit IgG ...
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bioRxiv - Microbiology 2022Quote: ... were used at dilution 1:20000 in blocking solution and anti-mouse IgG-HRP (A-9044, Sigma) at dilution 1:80000 were used for secondary antibodies ...
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bioRxiv - Pathology 2023Quote: ... or 2 mg of human IgG isotype (n = 4) (I4506-100MG, Millipore-Sigma, Saint Louis, MO,USA) were injected subconjunctivally in the upper eyelid of eyes immediately after the post-alkali exposure irrigation ...
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bioRxiv - Pathology 2023Quote: ... or 2 mg of human IgG isotype (n = 4) (I4506-100MG, Millipore-Sigma, Saint Louis, MO,USA) were injected subconjunctivally in the upper eyelid of eyes immediately after the post-alkali exposure irrigation ...
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bioRxiv - Molecular Biology 2023Quote: ... Then 0.2 mL of washed IgG Sepharose 6 Fast Flow beads (Millipore Sigma, Cat#: GE17-0969-01) were added to each sample and incubated on a rotisserie mixer for 0.5 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... and 9–11 nm colloidal gold conjugated to goat anti mouse IgG secondary antibodies (Sigma-Aldrich, #G7652). Following immunogold labeling ...
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bioRxiv - Microbiology 2023Quote: ... The following secondary antibodies were used in this study: goat anti-mouse IgG-AP conjugate (Sigma Aldrich) (dilution 1:6,000 in 5% w/v skimmed milk/TBS-T ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were detected with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:8,000, Merck Millipore) and visualised with the LiteAblot Plus substrate (Euroclone) ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated with a secondary antibody (anti-rabbit IgG; A7539, Sigma-Aldrich, St. Louis, MO) conjugated with alkaline phosphatase for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... Blots were incubated with ECL anti-rabbit IgG secondary antibody (Sigma, cat# GENA934-1 mL; 1:5000) at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: The following HRP-linked secondary antibodies were used: α-mouse IgG (NA931-1ML; Sigma-Aldrich, 1:5,000) and α-rabbit IgG (NA934-1ML ...