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Citations for Millipore Sigma :
1551 - 1600 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... blots were incubated for 1 h in 1:20,000 HRP-conjugated rabbit α-goat IgG (Sigma-Aldrich)/1% BSA/TBS at room temperature.
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bioRxiv - Molecular Biology 2023Quote: Background precipitation levels were determined by performing mock precipitations with a nonspecific IgG antiserum (Sigma-Aldrich C2288), and experiments were validated by qPCR on diagnostics regions before sequencing ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Microbiology 2024Quote: ... and 12.5 μl of the neat eluate or negative control commercially available purified human IgG (Sigma, I2511) in PBS at 200 μg/ml was used to resuspend the cells ...
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bioRxiv - Immunology 2024Quote: ... followed by addition of 50 μL of 1:750 anti-mouse IgG-HRP (Sigma-Aldrich, Cat. #A5278) in blocking buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Secondary antibodies were HRP-coupled anti-rabbit IgG (Sigma-Aldrich catalog number A 0545; dilution 1:5000) or HRP-coupled anti-mouse IgG (Sigma-Aldrich catalog number A0168 ...
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bioRxiv - Microbiology 2024Quote: ... and incubated 1 h with anti-rabbit-HRP or anti-rabbit-IgG-FITC (1:10,000; Sigma, France) in protein-free TBS blocking buffer ...
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bioRxiv - Microbiology 2024Quote: ... Binding was detected by incubating the plates sequentially with HRP-linked rabbit anti-human IgG (A8792, Sigma). After washing ...
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bioRxiv - Plant Biology 2024Quote: ... The following secondary antibody was used: anti-rabbit IgG (whole molecule)–HRP (A0545, Sigma, dilution 1:10,000).
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bioRxiv - Cancer Biology 2024Quote: ... from Cell Signaling Technology and anti-mouse IgG, (cat# A9044, dilution 1:2000) from Sigma Aldrich (1 hour at 37 °C). The membrane was analyzed using specific antibodies and visualized using an enhanced chemiluminescence 50 kit (Amersham Biosciences ...
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bioRxiv - Immunology 2024Quote: ... the serum IgG respone was detected using horseradish peroxidase (HRP) conjugated goat-anti mouse serum (A0186, Sigma) (1:42000 diluted in 2% MPBS-T ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized with 1:20,000 anti-rabbit IgG (whole molecule)-peroxidase secondary antibody (Sigma; A0545, lot 022M4811) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Secondary antibodies (1:2500 dilution of goat anti- rabbit IgG conjugated to HRP; Sigma; catalogue number A0545-1ML) were then added to membrane in TBS with 3% BSA and incubated at room temperature on a rocking platform for 2-4 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Detection was done with donkey anti-mouse IgG horseradish peroxidase at 1:5000 (1:5000, Millipore Cat#AP308P) and Luminata Crescendo Western HRP substrate (Millipore Cat#WBLUR0100) ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were incubated with HRP-linked secondary antibodies anti-mouse IgG (1:5000, A6782, Sigma-Aldrich, MO, USA) and anti-rabbit IgG (1:5000 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-GAPDH (1:2000, Protein Tech, #10494-1-AP) and anti-rabbit IgG-AP (1:20000, Sigma, #A3687)
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bioRxiv - Molecular Biology 2021Quote: ... followed by incubation with goat anti-mouse IgG conjugated to horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA) diluted 1 ...
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bioRxiv - Genetics 2019Quote: ... The bound anti-BrdU antibody was subsequently detected by anti-mouse IgG-FITC conjugate (Sigma, dilution 1:64) under a fluorescence microscope after mounting the ganglia in antifade (Sigma).
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... Bovine Serum Albumin and Goat Anti-Mouse IgG HRP conjugate were purchased from Millipore Sigma (St. Louis, MO). The mouse monoclonal antibody against P ...
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bioRxiv - Molecular Biology 2019Quote: ... transferred to PVDF, labeled with anti-HA (1:10,000, Pierce) and anti-mouse IgG-HRP (1:50,000, Sigma) and developed using ECL (GE Healthcare) ...
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A tyrosine kinase protein interaction map reveals targetable EGFR network oncogenesis in lung cancerbioRxiv - Cancer Biology 2020Quote: ... The supernatant was incubated with 60 μl preclearing beads (mouse IgG agarose, SIGMA or Sepharose 4FF, GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2019Quote: ... anti-rabbit IgG IR800CW (Odyssey 926-32211, 1:20,000), or anti-mouse IgG IR800CW (Odyssey 827-08364, 1:20,000) in 50% BLOK (Millipore), 0.1% Tween 20 in PBS ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... goat anti-rabbit IgG-HRP was added to the wells (100 μL/well; Sigma-Aldrich; dilution, 1:5000) and the plates were incubated at 37°C for 1 h ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... goat anti-rabbit IgG-HRP was added to the wells (100 μL/well; Sigma-Aldrich; dilution, 1:5000), and the plates were incubated at 37°C for 1 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Mouse monoclonal antibodies against human LMα3 (clone CL3112) and mouse IgG (both Sigma-Aldrich, St. Louis, Missouri, USA) were used at 0.5 μg mL−1.
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bioRxiv - Immunology 2021Quote: ... Foci were detected with an anti-mouse IgG conjugated to horseradish peroxidase at a 1:5000 dilution (Sigma), followed by True Blue substrate (KPL) ...
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bioRxiv - Bioengineering 2020Quote: ... The mouse anti-HA antibody (Covance, Princeton, NJ, USA) and goat anti-mouse IgG-alkaline phosphatase (Sigma-Aldrich) were added to the wells for incubation successively ...
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bioRxiv - Neuroscience 2020Quote: ... tissue was exposed to donkey anti-mouse IgG conjugated to Cy-3 in PB (1:80; EMD Millipore) for 1 h for visualization of Hu ...
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bioRxiv - Microbiology 2021Quote: ... Bound antibodies were probed using 50 μl/well of 1:1,000 of anti-mouse IgG HRP (Sigma–Aldrich) and 75 μl/well of tetramethylbenzidine substrate (Sigma–Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... using the mouse sera at dilution 1:1000 and anti-mouse IgG antibody conjugated to peroxidase (Sigma Aldrich), the antibody response specific to the EV-associated proteins was examined ...
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with a secondary Ab against immunoglobulin G (IgG) conjugated to horseradish peroxidase (Sigma-Aldrich) in PBST buffer (137 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies coupled to horseradish peroxidase used for immunoblotting were obtained from Sigma (A4914 Goat anti-Rabbit IgG).
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bioRxiv - Cell Biology 2020Quote: ... followed by Cy3-conjugated goat anti-rabbit IgG as the secondary antibody (Cat # AP132C, Millipore Sigma, Burlington, MA). The irradiation effect on tendon cells was detected with a live/dead cell viability assay kit (Cat # L-7013 ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-mouse or anti-rabbit IgG coupled to peroxidase were used as secondary antibodies (Millipore, 1:5000 dilution). Western bright ECL substrate or western bright Sirius-femtogram HRP (Advansta ...
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bioRxiv - Neuroscience 2020Quote: ... were incubated with secondary antibodies for 2 h: Alexa Fluor 488 goat anti-mouse IgG (1:400, Millipore), or Fluor 594 goat anti-rabbit IgG (1:400 ...
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bioRxiv - Bioengineering 2019Quote: ... and 10 nm colloidal gold-conjugated goat anti-mouse IgG (1:40; G7652; Sigma Aldrich; St. Louis, MO). These methods were partially adapted from a protocol by Rojas and colleagues (15).
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bioRxiv - Cancer Biology 2019Quote: ... F9 and WEHI-164 tumors was studied on ice-cold acetone-fixed 8-10μm cryostat sections stained with FITC labeled IgG(L19) (FITC_F7250 Sigma) (final concentration 2μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG antibodies and the Chemiluminescent Peroxidase Substrate (Sigma-Aldrich, 34578) were used ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were then washed and incubated with the goat anti-rabbit IgG/HRP conjugate (1:5000, Sigma-Aldrich). The signal was detected with a Pierce™ ECL detection kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... the grids were rinsed with blocking buffer and incubated with 10 nm gold-conjugated anti-rabbit IgG (Sigma), diluted 1:20 in blocking buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... either 1 μl of rabbit α-Bru1 (gift from A. Ephrussi) or 2 μg of rabbit IgG (Millipore) were incubated with ovarian lysate over night at 4°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... UBC (42)) diluted 1:5000 in TBST followed by Goat Anti-Rabbit IgG antibodies (HRP-conjugate, Sigma-Aldrich) diluted 1:5000 in TBST.
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bioRxiv - Immunology 2021Quote: ... the plate was washed four times and incubated with HRP conjugated anti-horse whole IgG secondary antibody (Sigma) for 1 hr at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... Background precipitation levels were determined by performing mock precipitations with a non-specific IgG antiserum (Sigma C-2288) and were only a fraction of the precipitation levels obtained with the specific antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... After the reaction with alkaline phosphatase-labelled goat anti-rabbit IgG (Sigma; 1:1,000 dilution in dilution buffer), immunoreactive products were obtained in a substrate solution of p-nitrophenylphosphate (SIGMA FAST™ p-nitrophenyl phosphate tablet set ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated with anti-mouse IgG conjugated with alkaline phosphatase (whole molecule) (Sigma-Aldrich A-3688) diluted 1:2000 ...
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bioRxiv - Immunology 2021Quote: ... 50 µl/well of the alkaline phosphatase-conjugated anti-mouse IgG was added (Sigma-Aldrich, St Louis, MO), it was diluted at 1:1000 in a PBS/BSA 2% solution ...