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Citations for Millipore Sigma :
1951 - 2000 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
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bioRxiv - Neuroscience 2022Quote: ... We combined this immunohistochemistry with the one for serotonin (5-HT) using the rabbit IgG anti-5-HT (1/500, Sigma-Aldrich, 49545) and the goat anti-rabbit Fluor Alexa 568 (1/500 ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... then three times for 5 minutes each then placed in Donkey Anti-Guinea Pig IgG Antibody-HRP Conjugate (Millipore-Sigma Burlington, MA) diluted 1:10,000 in Bløk-CH Noise Cancelling Reagent for 1h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... the treated cells were performed using anti-chicken IgY (IgG) (whole molecule)-FITC antibody produced in rabbit (Sigma–Aldrich, dilution 1:640) as the secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... followed by incubations with corresponding horseradish peroxidase (HRP)-conjugated goat polyclonal anti-mouse IgG (1:5,000 dilution, EMD Millipore Etobicoke, Ontario, Canada). Blots were developed with Supersignal West Femto enhanced chemiluminescence substrate (Thermo Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Plant Biology 2023Quote: ... The pollen was then washed 3 times in 1x PBS and incubated in a 1:1000 dilution of rabbit anti-rat IgG FITC (Sigma, F1763-.5ML) in 1x PBS+MP for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHH-Fcs or conventional human monoclonal antibodies was detected by HRP-conjugated rabbit anti-human IgG (Sigma, A8792, 1/2000). After washing 50 μL of TMB substrate (Tetramethylbenzidine ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue morphology was visualized using fluorescent antibodies specific to lymphocyte and neuron specific molecular markers (anti-CD45 [Novus], anti-CD3 [Primary, Bio-Rad], Secondary anti-Rat IgG, [ThermoFisher], and anti-NeuN [Millipore Sigma] ...
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bioRxiv - Systems Biology 2023Quote: ... Mock RNA co-IP controls were prepared in an identical manner using either naïve polyclonal mouse IgG (control for α-ORF1p; Millipore Sigma, #I5381) or naïve polyclonal rabbit IgG (control for α-ORF2p ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with primary antibody (15 µg/mL in 3% BSA in PBS): rabbit anti-KCNN4 antibody (IgG, clone HPA053841, Sigma Aldrich®) or control rabbit antibody (IgG ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in RPMI-1640 complemented with 10% IgG-free Fetal Calf Serum (Sigma-Aldrich, #F1283) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... 137 mM NaCl2) before blocking for 1 hour at RT with blocking solution (2% BSA IgG free and protease free (Sigma-Aldrich, A3059); 0.1 % Tween 20 (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated for 2 h at RT with secondary antibodies Anti-Rat-IgG conjugated with alkaline phosphatase (AP) (Sigma-Aldrich, USA) at a dilution of 1:1000 in 2.5% BSA in PBS ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with CR3022 anti-S antibody (1 ug/mL)128 overnight followed by anti-human IgG-HRP-conjugated goat secondary antibody (Sigma, 1:500) for 2 h ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... the slide was washed three times with the wash buffer and stained sequentially with the Immunol Fluorence Staining Kit with Alexa Fluor 647-Labeled Goat Anti-Rabbit IgG (P0180, Beyotime, China) and 4’,6-diamidino-2-phenylindole (DAPI, MBD0015, Sigma-Aldrich, USA) as per the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Yhb1-TAP and α-tubulin proteins were detected using the polyclonal anti-mouse IgG antibody (ICN Biomedicals) and monoclonal anti-α-tubulin antibody (clone B-5-1-2; Sigma-Aldrich), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysate was incubated with RIP binding buffer containing magnetic beads coated with human Ago2 antibody or mouse IgG antibody (control) (Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation of chromatin preparations was performed using rabbit anti-CLAMP antibody (Rieder et al., 2017) and a polyclonal rabbit IgG (Millipore-Sigma, 12-370). qPCR was performed as described in (Urban et al. ...
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bioRxiv - Genomics 2020Quote: ... Primary antibodies were detected using anti-rabbit secondary antibodies (1:5000 dilution, Anti-Rabbit IgG HRP Linked Whole Ab Sigma Cat# GENA934-1ML). The band intensities were quantified with Image J (Schneider et al ...
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bioRxiv - Microbiology 2022Quote: ... The control Mbl protein was visualized using primary rabbit anti-Mbl antibodies (dilution 1:10,000) and the secondary goat peroxidase-coupled anti-rabbit IgG antibodies A0545 (Sigma-Aldrich; dilution 1:10,000). Three independent experiments were performed ...
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bioRxiv - Genetics 2019Quote: ... FER was detected using anti-FER (rabbit polyclonal, 1:1000; Xiao, Stegmann et al., under revision) and anti-Rabbit IgG (whole molecule)–HRP (Sigma A0545, 1:5000).
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bioRxiv - Plant Biology 2019Quote: ... raised against gel-purified ATLP1 using alkaline phosphatase(AP)-conjugated goat anti-rabbit IgG (1:10000 dilution) as the secondary antibody and 5-bromo,4-chloro,3-indolylphosphate(BCIP)/nitroblue tetrazolium (NBT)(Sigma Chemical Co. MS) as the substrates (Safadi et al. ...
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bioRxiv - Microbiology 2020Quote: ... The anti-Psa antibodies (see below) were used at 1:5,000 dilution and the Anti-Rabbit IgG-Peroxidase antibody (Sigma-Aldrich, St Louis, MO) was used at 1:10,000 dilution ...
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bioRxiv - Biochemistry 2022Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich, Cat No A2179) in PBST ...
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bioRxiv - Immunology 2020Quote: ... a pooled rhesus serum collected day 14 after infection with SARS CoV-2 was used as standard after depletion of IgG using GE Healthcare Protein G Sepharose (Sigma, St. Louis, MO) as described (52) ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed three times in 1x TBST and then incubated for 1 hour at 22°C in anti-mouse IgG HRP conjugate secondary antibody (Sigma, 2834819; 1:1000). Membranes were washed three times in 1x TBST before adding enhanced chemiluminescence detection reagent (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... anti-rabbit IgG (goat, coupled to Alexa 488, Thermo Fisher, A32731, 1:500 and donkey, coupled to Cy3, Merck Millipore, AP182C, 1:1000), anti-goat IgG (donkey ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were subsequently incubated for 3 h at room temperature with a biotinylated anti-rabbit IgG antibody (1:1000, EMD Millipore, #AP132B, RRID:AB_11212148) followed by 1 h with avidin-biotin complex (ABC Vector Laboratories ...
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bioRxiv - Biophysics 2019Quote: ... Permeabilization of the membrane and blocking of unspecific binding was achieved by adding 5 % (w/v) bovine serum albumin (IgG-free, Carl Roth, Karlsruhe, Germany) and 0.3 % (w/v) Triton-X 100 (Sigma-Aldrich, St. Louis, USA) in PBS for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Permeabilized platelets were incubated with 1:500 dilution of mouse monoclonal anti-α-Tubulin IgG (Clone DM1A, Cat.No. T9026, Sigma Aldrich GmbH, Germany) primary antibody diluted in 0.5% saponin with 0.2% BSA in PBS for 16 hours at 4°C followed by three washing steps in PBS for 5 min each ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2022Quote: ... Coverslips were incubated with primary antibodies diluted in 5 % BSA in 1x PBS: Tau1 clone 46 (1:500) mouse monoclonal IgG (Sigma-Aldrich, T-9450), GFP (H-140 ...
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bioRxiv - Plant Biology 2022Quote: ... a 1:5000 dilution of anti-rabbit IgG secondary antibodies was employed and detected by chemiluminescence with the Luminata pack (Millipore, Billerica MA, USA).
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bioRxiv - Genomics 2022Quote: ... were added in addition to 5 μg of anti-N6-methyladenosine antibody (see above for catalog numbers) or Normal Rabbit IgG (Sigma Millipore, NI01-100UG). Samples were incubated for 12 hrs on a rotator at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... IgG assay samples were incubated with 100 μL of pre-washed 50/50 Protein Sepharose A slurry (Sigma-Aldrich, diluted in lysis buffer) for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... plates were washed again as above and 100 μl of a goat anti-rabbit IgG (H + L) alkaline phosphatase conjugate (Sigma, St. Louis, MO) diluted 1:2,000 in PBS/1% NFM were added to each well and incubated for 1.5h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in serum IgG free RPMI-1640 (Sigma-Aldrich, MO, USA) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Pathology 2024Quote: ... The membranes were incubated after washing with an anti-rabbit IgG alkaline phosphatase secondary antibody (1:5000, Sigma– Aldrich, St Louis, MO, USA) for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were then washed three times with TBST and incubated with peroxidase-conjugated anti-mouse IgG secondary antibody (1:4,000 dilution) (Sigma-Aldrich; catalog no. A4416) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2024Quote: The sample was incubated overnight (12Lhours) at 4 °C with a primary mouse anti alpha- and beta-tubulin IgG antibodies (Sigma-Aldrich (REF: T5168) and Sigma-Aldrich (REF ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation in either sheep anti-mouse or donkey anti-rabbit IgG-conjugated with horseradish peroxidase (HRP) (Millipore Sigma, GENA931, GENA934, 1:4,000) in TBST at room temperature for two hours ...
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bioRxiv - Biochemistry 2020Quote: ... Ribosomal protein L3 was detected using anti-L3 primary antibodies (a gift from Fujio Kawamura; 1:20,000 dilution) combined with goat anti-rabbit IgG-HRP secondary antibodies (Sigma-Aldrich, A0545; 1:10,000 dilution). ECL detection was performed using WesternBright™ Quantum (K-12042-D10 ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were rinsed with the blocking solution and incubated with secondary antibodies for 2 h at room temperature (FITC-conjugated anti-rabbit IgG, AP132F, Millipore, Darmstadt, Germany; 1:200). The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories ...
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bioRxiv - Physiology 2022Quote: ... membranes were sequentially incubated with primary antibody (a 1:1000 dilution) for 16 hours at 4 °C and then biotin-conjugated goat anti-rabbit IgG (Millipore-Sigma/Chemicon, Oakville, ON, Canada) secondary antibody (1:10,000 dilution ...