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Citations for Millipore Sigma :
1851 - 1900 of 2031 citations for Fab IgG SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with horseradish peroxidase (HRP)-conjugated goat polyclonal anti-mouse IgG (1:5,000 dilution, EMD Millipore Etobicoke, Ontario, Canada). Supersignal West Femto enhanced chemiluminescence substrate (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 4 times for 10 min at RT and then incubated with the secondary antibodies directed against goat IgG coupled to horseradish peroxidase (1:100,000 – Sigma, Munich, Germany) dissolved in the blocking solution ...
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bioRxiv - Microbiology 2020Quote: ... the bound NoV capsid proteins were detected with mouse anti norovirus GII (1:1000; NativeAntigen) and by adding horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma Aldrich). Horseradish peroxidase activity was detected with a TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Immunology 2021Quote: ... antibody diluted 1:1000 in blocking solution or rabbit Anti-Monkey IgG (whole molecule)-Peroxidase antibody (Sigma-Aldrich Cat# A2054, RRID:AB_257967) were added to each well ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG (Jackson Immuno Research 109-036-088) or anti-human IgA (Sigma A0295) secondary antibody conjugated to horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2020Quote: ... the primary antibodies were discarded and the sections probed with secondary antibody (anti-rabbit IgG conjugated to FITC) (Sigma-Aldrich, Germany) prepared at a dilution of 1:10000 in PBST ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The following day plates were washed three times with TBS-T and incubated for 2 hours at room temperature with secondary antibody horseradish peroxidase-conjugated rabbit anti-horse IgG (Sigma A6917), diluted to 1 in 1,000 in PBS ...
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bioRxiv - Molecular Biology 2019Quote: ... the membranes were washed with TBST buffer for three times and subsequently incubated in 1:10,000 dilution of secondary antibody (anti-mouse IgG whole molecule conjugated with alkaline phosphatase) (A3562, Sigma-Aldrich, USA). The membranes were then washed with TBST buffer for three times and detected using AP detection kit (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... PVDF membranes were incubated with peroxidase-conjugated goat anti-rabbit IgG or goat anti-mouse IgG (1:5000, ZSGB-BIO, China) for 1 hours at room temperature and were visualized by chemiluminescence reagent (Millipore, USA). GAPDH served as the reference gene ...
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bioRxiv - Microbiology 2019Quote: ... The TopA protein was subsequently detected using primary rabbit polyclonal anti-TopA antibodies and mouse secondary anti-rabbit IgG antibodies conjugated with alkaline phosphatase (Sigma Aldrich), according to the procedure described previously (Szafran et al. ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with the secondary antibody (anti-Mouse-HRP IgG, 1:10,000, Sigma-Aldrich, Saint Louis, MO, USA) diluted in TBS-T for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Microbiology 2020Quote: ... The secondary solution was prepared with 1% non-fat milk in PBS-T using anti-mouse IgG (whole molecule)–alkaline phosphatase (AP) antibody produced in goat (Sigma-Aldrich) at a dilution of 1:3,000 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Immunology 2019Quote: ... Each plate contained a standard curve with 2-fold dilutions of purified mouse IgG or IgA (Sigma Aldrich, St. Louis, MO) starting at 2,000 ng/mL ...
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bioRxiv - Immunology 2019Quote: ... the plates were washed with PBS four times and horseradish peroxidase-conjugated anti-mouse IgG or horseradish peroxidase-conjugated anti-mouse IgA (Sigma Aldrich) diluted 1 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The titre is described as the dilution at which absorbance was greater than of the negative control (IgG from non-immunised horses; Sigma, UK) plus two standard deviations.
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bioRxiv - Microbiology 2019Quote: ... alkaline phosphatase-conjugated (1:10,000) or goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated (1:10,000) secondary antibody (Sigma, St. Louis, MO). Immunoreactive bands were detected by the addition of NBT/BCIP (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... An NS (nanostructured) or FT (flat) film device was placed in contact with cells on apical side along with FITC-IgG (Sigma #F9636) supplemented to the cell imaging medium at 10 μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 90 min with either horse-radish peroxidase (HRP) conjugated goat anti-rabbit IgG (1:10000; EMD Millipore) or HRP conjugated goat anti-mouse IgG (1:10000 ...
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bioRxiv - Cell Biology 2020Quote: ... OMP-18R5 (OncoMed Pharmaceuticals, Redwood City, CA) at 10μg/mL with antihuman IgG (Fc) secondary conjugated to FITC (Millipore Sigma AP113F).
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bioRxiv - Microbiology 2020Quote: ... grids were incubated for 1 hour 37 °C with a 1:2000 dilution of a 10 nm gold-labelled goat anti-rabbit IgG (G7277; Sigma Aldrich), washed with 1x PBS ...
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bioRxiv - Immunology 2021Quote: ... was used at a 1:5000 dilution (T84) or horseradish peroxidase-conjugated goat anti-rabbit IgG secondary antibody (A0545, Sigma-Aldrich) at 1:2000 dilution ...
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bioRxiv - Immunology 2021Quote: ... SARS2-16 (hybridoma supernatant diluted 1:6,000 to a final concentration of ∼20 ng/mL) and then HRP-conjugated goat anti-human IgG (Sigma-Aldrich, A6029) in PBS supplemented with 0.1% (w/v ...
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bioRxiv - Immunology 2021Quote: ... and then monoclonal anti-6x(HIS)-tag antibody was probed followed by detection with anti-mouse IgG conjugated with alkaline phosphatase (A3562: 1ml Sigma, USA) was used for developing the blots.
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bioRxiv - Immunology 2020Quote: ... Mouse antibodies were detected by incubation with polyclonal goat anti-mouse IgG-horseradish peroxidase conjugate (Sigma-Aldrich, St. Louis, MO, USA). The plate was developed with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Spore suspensions were then incubated for 1□h with 1:20 donkey anti-mouse IgG antibody coupled to 12-nm gold particles (Sigma G7777) in PBS–1% BSA for 1□hr at RT ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Biochemistry 2022Quote: ... The following secondary antibodies diluted to 1:6000 in 3% milk and applied for two hours at room temperature: goat anti-Rabbit IgG peroxidase (Sigma #A0545) and goat anti-mouse IgG peroxidase (Sigma #A0168) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed 6 times with washing buffer and then incubated with anti-human IgG secondary antibody conjugated to horseradish peroxidase (HRP) (Jackson Immuno Research 109-036-088 109-035-129 and Sigma A0295) in blocking buffer at a 1:5,000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... mice were intraperitoneally injected every 48 h with 200 µg monoclonal anti-Ly6G antibody (clone 1A8; Leinco) or 200 µg polyclonal rat serum IgG (Sigma-Aldrich) diluted in sterile PBS beginning at 8 dpi and ending at 28 dpi.
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bioRxiv - Immunology 2023Quote: ... ASCs obtained through magnetic selection (on average >2 × 106 cells per spleen and >1.5 × 106 cells for bone marrow) were washed, resuspended in encapsulation buffer (PBS, 10% ultra-low IgG FBS, 18% OptiPrep (Sigma Aldrich), 2 mM EDTA ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were blocked with PBS 1x-1% non-fat milk overnight at 4°C and incubated in primary antibody anti-Orco (1:500) and the secondary antibody Goat anti-rabbit IgG HRP conjugate (1:5000 Sigma-Aldrich) for 1 hour each ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Microbiology 2023Quote: ... Goat anti-IgG rabbit alkaline phosphatase conjugated secondary antibody (A9919) and goat anti-IgG mouse alkaline phosphatase conjugated secondary antibody (A2429) were purchased from Sigma Aldrich.
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bioRxiv - Bioengineering 2023Quote: ... Antibody binding was detected using HRP-conjugated anti-rabbit IgG and visualized with Immobilon Western Chemiluminescent HRP Substrate (Millipore, Burlington, MA) and an ImageQuant LAS4000 digital imaging system (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Mock RNA coIP controls were prepared in an identical manner using naïve polyclonal mouse IgG (control for αORF1p; Millipore Sigma, #I5381) or naïve polyclonal rabbit IgG (control for αORF2p ...
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bioRxiv - Biochemistry 2022Quote: ... The produced PSGL-1/mIgG2b were purified form the cell culture supernatants using goat anti-mouse IgG agarose beads (Sigma–Aldrich). Each enzyme (2 µM ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Microbiology 2022Quote: ... the blots were incubated at room temperature on a rocker for 1 hr with secondary anti-mouse IgG-HRP (Sigma, A4416) or anti-rat IgG-HRP (Thermo ...
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bioRxiv - Microbiology 2023Quote: ... whole cell lysate (acquired as described above) from transfections was incubated overnight at 4°C with mouse IgG agarose (Sigma A0919). The agarose was then removed by centrifugation and the lysate recovered and incubated overnight at 4°C with either anti-G/CFP monoclonal antibody agarose (MBL International D153-8 ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were probed with a 1:10,000 dilution of alkaline-phosphatase-conjugated goat anti-mouse IgG secondary antibody (Sigma-Aldrich (A2179)) in PBST ...
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bioRxiv - Cancer Biology 2023Quote: ... washed with complete T cell medium supplemented with 1% (v/v) FBS and then TCR cross-linked with goat α-hamster IgG (20 μg /ml, Sigma) for the indicated times at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated in primary antibody solution containing Anti-Neurofilament 200 primary antibody produced in rabbit IgG (N4142, Sigma Aldrich) and biotin-conjugated Anti-NeuN primary antibody produced in mouse IgG (MAB377B ...
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bioRxiv - Microbiology 2023Quote: ... wells were incubated with anti-mouse IgG peroxidase-conjugated (1:1000 in PBS + 1% BSA, Sigma Aldrich, St. Louis, MO, USA) at RT for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was the anti-mouse IgG (whole molecule)–peroxidase antibody produced in rabbit (Sigma; cat# A9044; 1:10000 dilution).
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bioRxiv - Microbiology 2023Quote: ... anti-p-PKC-δ-T505 (AP0776) followed by the respective secondary antibodies (horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG; Sigma). Mouse-monoclonal anti-β-actin-peroxidase antibody (A3854 ...
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bioRxiv - Biochemistry 2023Quote: ... and detected by standard western blotting procedures using rabbit anti-sera against IbpA (Eurofin) as primary antibody and HRP-conjugated anti-rabbit IgG as secondary antibody (Sigma-Aldrich). For IbpACn and IbpAVh detection ...