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Citations for Millipore Sigma :
8051 - 8078 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: 50 mkL of cell suspension from section 2.1 was pelleted and suspended in 50 mkL of 10 mM or 50 mM L-NAME solution in water (Sigma-Aldrich, St. Louis, MO, USA). The samples were incubated in a ThermoMixer for 1 h at 30° ...
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bioRxiv - Biochemistry 2021Quote: ... 0.072 M K2HPO4) in a 2 L baffled flask supplemented with required antibiotics and 200 µL of Antifoam 204 (Sigma-Aldrich Inc., St. Louis, Missouri, USA). Large-scale cultures were incubated at 37°C (BL21(DE3) ...
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bioRxiv - Systems Biology 2020Quote: ... Crypts were then cultured in a Matrigel culture system (described below) in small intestinal crypt medium (50X B27 supplement, Life Technologies, 1 mM N-acetyl-L-cysteine, Sigma-Aldrich, in basal culture medium) supplemented with differentiation factors at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2019Quote: ... 300 µl of 10% methanol (LC-MS grade) spiked with stable isotope-labelled amino acids (L-amino acid mix, Sigma-Aldrich, Co., St. Louis, MO, USA) was added to each sample ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were plated at a density of 40,000/cm2 on dishes precoated with poly-l-ornithine and fibronectin (both from Sigma-Aldrich, St. Louis, MO, USA; Stockholm, Sweden). Cells were kept in enriched N-2 medium with 10 ng/ml of basic fibroblast growth factor (R&D systems ...
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bioRxiv - Microbiology 2020Quote: ... Aqueous solutions of reduced glutathione (Fisher, 10 mg/ml) L-ascorbic acid sodium salt (Acros; 10 mg/ml) or uric acid potassium salt (Sigma; 10 mg/ml in 1M NaOH) were prepared and added to 1.5% w/v agar-containing DDM at final concentrations of 0.1 mg/ml ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was suspended in 1 mL SP4 liquid medium and 300 μL were plated onto selective SP4 solid medium supplemented with 50 µg/mL gentamicin or 5 μg/mL puromycin (Sigma-Aldrich, Saint-Louis, MO, United States) and incubated at 37°C for 3 to 5 days ...
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bioRxiv - Microbiology 2021Quote: ... Bacillus cultures were adjusted to 2*10−2 or 8*10−4 at OD600 and 5-fold dilution series were prepared with 6 steps using peptone saline as diluent ([Millipore]; Maximum Recovery Diluent 9.5 g/L). 15 μl of each bacterial dilution was inoculated in consecutive wells by spotting the solution in the center of the wells ...
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bioRxiv - Immunology 2020Quote: ... Creatine (Sangon Biotech cat. A600326, 10 mg/ml), L-Citrulline (Sangon Biotech cat. A604057, 5 mg/ml) and Spermidine (Sigma-Aldrich cat. S0266, 2 mg/ml) were added to the drinking water 7 days prior to the IMQ application and the treatment lasted until the end of model establishment ...
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bioRxiv - Neuroscience 2020Quote: ... were cut on a cryostat and every sixth section was counterstained with toluidine blue pH 4.0 (1 g/l Toluidine Blue, Sigma, 198161 in 0.8 M glacial acetic acid). Digital images were captured at a 2.5x magnification (Canon EOS 450D digital camera ...
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bioRxiv - Cell Biology 2024Quote: ... the media contained 0.17 g/L yeast nitrogen base without ammonium sulfate (#1553 Conda Pronadisa, Madrid, Spain) and 1 g/L of monosodium glutamic acid (Sigma-Aldrich, St. Louis, MO, USA #G1626). The strains were selected using a dropout mix (same composition as “SD” above ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were grown in DMEM/Ham’s F-12 (1:1 mixture) (BIOCHROME, Germany) supplemented with 10 % fetal calf serum (FCS) and 4 mM L-Glutamine (Sigma Aldrich GmbH St Louis, MO, USA) at 7% CO2 and 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... HEK293 cells were cultivated in DMEM with Na-pyruvate (BIOCHROME, Germany) supplemented with 10 % fetal calf serum (FCS) and 4 mM L-Glutamine (Sigma Aldrich GmbH St Louis, MO, USA) at 7% CO2 and 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... pH 7.5; 34 mM lithium citrate; 50 mM l-cysteine; 15% [wt/vol] PEG 8000; and 1 μM papain [Millipore Sigma, St. Louis, MO; Cat. No. P3125-100MG]). After incubating cells for 30-40 min ...
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bioRxiv - Developmental Biology 2022Quote: ... counted and seeded at a density of 55000 cells/cm2 onto Poly-L-Lysine/Laminin/Fibronectin (Sigma-Aldrich, 100 µg/ml, 2 µg/ml, 2 µg/ml) coated plates or coverslip in neuronal maturation medium supplemented with ROCK inhibitor Y27632 (10uM ...
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bioRxiv - Plant Biology 2022Quote: ... 5 μL of supernatant was analyzed by SDS-PAGE and subjected to immunoblot analysis with mouse anti-Myc (clone 4a6, Millipore, cat. no. 05-724, dilution 1:1000) and goat anti-mouse IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then delipidated with clear unobstructed brain imaging cocktail and computational analysis (CUBIC)-L clearing solution (10 wt% N-buthyldiethanolamine [[#471240, Sigma-Aldrich, Germany] and 10 wt% Triton X-100 [Sigma-Aldrich, Germany] in Milli-Q water)49,50 ...
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bioRxiv - Bioengineering 2023Quote: ... the basal medium was changed to LaSR medium (Advanced DMEM/F-12, Life Technologies, cat# 12634, GlutaMAX, Thermo Fisher, cat# 35050-061, 2.5 mM, and L-ascorbic acid, Sigma-Aldrich, cat# A8960, 60 μg/mL) supplemented with CHIR99021 (Selleck ...
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bioRxiv - Biochemistry 2024Quote: ... Approximately 75,000-100,000 cells were plated on 5 mm diameter coverslips (Word Precision Instruments cat# 502040; coated overnight with 0.1 mg/mL poly-L-lysine hydrobromide [Millipore Sigma cat# P9155], and washed 3x with HBSS) and placed in 96-well plates containing 200 μl of complete neuro DMEM for 4 h ...
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bioRxiv - Neuroscience 2023Quote: ... Nunc glass bottom dishes with an inner diameter of 27 mm were coated with Poly-L-Ornithine (Sigma-Aldrich P4957, 0.01% stock diluted 1:3 in PBS) overnight at 37° C ...
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bioRxiv - Neuroscience 2024Quote: ... microglia were harvested by mild shaking and collected (10 minutes at 800g) and replated into culture plates previously coated with poly-L-ornithine (Sigma-Aldrich, 0.1 mg/mL in borate buffer). Microglia were allowed to attach to the new flasks for 16 hours and treated with 100nM aSyn species for 24 hours.
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bioRxiv - Neuroscience 2024Quote: ... the eggs were moved to a hatching tank with a small volume of ice-cold hatching solution (Humic acid, Sigma-Aldrich, 53680, 1g/L in aquarium water) with a continuous flow of oxygen (Polačik et al. ...
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bioRxiv - Neuroscience 2020Quote: ... mice were food deprived for the 6 hr during the day before receiving a bilateral parenchymal injection of L-leucine (Sigma, 2.1 mM, 50 nl/side, 50 nl/min) or aCSF (R and D ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5 mL of papain digestion buffer (100 mm sodium phosphate buffer containing 10 mm L-cysteine [Sigma-Aldrich], 125 μg mL−1 papain [Sigma-Aldrich], and 5mmNa2EDTA [Sigma-Aldrich] in ddH2O at pH 6.5) was added to the spheroids that were placed on a rotator in an oven at 60 °C overnight ...
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bioRxiv - Neuroscience 2022Quote: ... and Mannitol (3 g of Bacto Peptone [Becton Dickinson], 5 g of Yeast Extract [Sigma-Aldrich], 25 g of D-Mannitol [Sigma-Aldrich], 1 L of Milli-Q water). Liver Infusion Broth (Becton Dickinson) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: HEK cells used for electrophysiological study were adhered to the poly-L-lysine-coated (MW 30,000-70,000, Sigma-Aldrich; 0.1 mg/mL for 2 hr at 37 ºC) coverslips (CS-5R ...
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bioRxiv - Developmental Biology 2022Quote: ... counted and seeded at a density of 55000 cells/cm2 onto Poly-L-Lysine/Laminin/Fibronectin (all from Sigma-Aldrich, 100 µg/ml, 2 µg/ml, 2 µg/ml) coated plates or coverslip in neuronal maturation medium supplemented with ROCK inhibitor Y27632 (10uM ...
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bioRxiv - Neuroscience 2019Quote: ... we used 10 μl amyl acetate (AM, Sigma Aldrich cat. no.: 109584; CAS No.: 628-63-7; diluted 1:250 in paraffin oil, Sigma Aldrich cat. no.: 18512, CAS no.: 8012-95-1) and benzaldehyde (BA ...