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Citations for Millipore Sigma :
7701 - 7750 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... which was un-exposed European pooled serum.23 Secondary detection was with Human IgG (H&L) Antibody Peroxidase Conjugated (Sigma – Aldrich, Germany), developed with Pierce™ TMB Substrate Kit (Thermo Fischer ...
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bioRxiv - Neuroscience 2020Quote: ... The middle region of the cochlea was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore #P4957, USA) and laminin (50 μg/mL ...
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bioRxiv - Neuroscience 2020Quote: Primary hippocampal neurons were isolated from P1 C57BL/6 mice by dissociating with 0.25% trypsin and plated onto poly-L-lysine (Sigma-Aldrich, MO, USA)-coated culture dish ...
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bioRxiv - Microbiology 2021Quote: ... we transferred 1/100 volume of overnight culture to fresh LB-Amp media that additionally contained 1% L-arabinose (w/v) (Sigma-Aldrich #A3256) to induce the expression of Lambda Red recombinase ...
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bioRxiv - Immunology 2020Quote: BS-C-1 (ATCC® CCL-26™) cells were cultured in Eagle’s minimum essential medium (EMEM) supplemented with 2 mM L-glutamine (Sigma Aldrich), antibiotics (penicillin ...
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bioRxiv - Microbiology 2021Quote: ... phosphocholine chloride calcium salt tetrahydrate and L-α-Phosphatidylinositol (∼50% TLC) from Glycine max (soybean) ∼50% (TLC) were also purchased from Sigma-Aldrich, Merck ...
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bioRxiv - Microbiology 2021Quote: ... intestinal growth medium (DMEM F12 [Gibco, Gaithersburg, MD], 1× B27 supplement [Thermo Fisher Scientific], 2-mM L-glutamine, 1% penicillin-streptomycin, 40 mM HEPES [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2019Quote: ... DT40 cells (immortalised chicken B cell line [16]) were maintained in Roswell Park Memorial Institute (RPMI) media supplemented with L-glutamine (Sigma-Aldrich, Merck), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... media was changed to serum-free media (DMEM, 2mM L-glutamine) overnight followed by insulin stimulation (100nM human insulin, Sigma-Aldrich, I9278), with 30-minute vehicle ...
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bioRxiv - Cell Biology 2021Quote: HC-11 cells were routinely cultured in complete growth medium: RPMI 1640 (50-020-PC, Mediatech Inc.) supplemented with 2 g/L sodium bicarbonate (S5761-500G, Sigma Life Science), 100 U/mL penicillin ...
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bioRxiv - Biochemistry 2022Quote: ... cells were detached and re-plated at 60,000 cells per well into a poly-L-ornithine-coated (Sigma-Aldrich; cat. no. P3655) white 96-well plate (Thermo scientific ...
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bioRxiv - Biophysics 2022Quote: 20 μL of a solution containing AcmJRL (20 μM) and SyproOrange (final concentration 10x of a 5000x stock in DMSO, Sigma-Aldrich, Germany) in TBS (150 mM NaCl ...
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MEK inhibitor resistance in lung cancer cells associated with addiction to sustained ERK suppressionbioRxiv - Cancer Biology 2022Quote: ... MK-2206 (Selleckchem, S1078), NSC 23766 (Selleckchem, S8031), dabrafenib (Selleckchem, S8031), infigratinib (Selleckchem, S2183) and N-acetyl-L-cysteine (Sigma-Aldrich, A7250) were added to cells when indicated ...
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bioRxiv - Cancer Biology 2022Quote: ... centrifuged and re-suspended in a 250 µl aliquot of the pLVX-EF1α-turboGFP-WPRE-Neo lentivirus with 10 µg/ml polybrene infection/transfection reagent (Millipore-Sigma, TR-1003), transferred into a single well of a low attachment 48-well plate and centrifuged for 1 h at room temperature then incubated at 37°C 5% CO2 for 6 hours ...
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bioRxiv - Plant Biology 2021Quote: ... 15 µl CaCl2 (to a final concentration of 0.5 M) and 7.5 µl of 20 mg/ml Protamine (Sigma-Aldrich, St. Louis, USA) were added and the solution was incubated at RT for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Heparin (20 μl of 50 mg mL−1) was reacted with 20 μl hydroxylamine-biotin in 40 μl 300 mM aniline (Sigma-Aldrich, UK) and 40 μl 200 mM acetate pH 6 for 48 h at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... A soil subsample of 75 g was stirred into 1 L of 2.5 mM MES monohydrate (Sigma Aldrich, St. Louis, MO, USA) in sterile distilled water (diH2O ...
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bioRxiv - Microbiology 2021Quote: ... The cells were resuspended in 1 mL base SmOx media with 100 μM of reduced L-glutathione (Sigma-Aldrich, St. Louis, MO). A 125 μL volume of cells containing 1 x106 were plated in Lab-Tek II chambered coverglass #1.5 Borosilicate (Nunc International ...
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bioRxiv - Molecular Biology 2020Quote: ... at 200 V using 1 X NuPAGE Running Buffer [50 mL of 20 × 1 L Stock [209.2 g MOPS (Sigma-Aldrich, M1254-1KG), 121.1 g Bis-Tris (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The gels were then transferred to Polyvinylidene fluoride membranes at 85V for 1 hour using 1 X NuPAGE Transfer Buffer [50 mL of 20 × 1 L Stock [81.6 g Bicine (Sigma-Aldrich, B3876-250G), 104.8 g Bis-Tris (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... cells were again dissociated with Accutase and re-plated into a 96-well plate coated with poly-L-ornithine hydrobromide (PLO, 0.1 mg/mL, Sigma-Aldrich Cat. #P3655), with 30,000 cells per well ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were then treated overnight in 1 volume of 100 % methanol and l/3 volumes of 100 % dichloromethane (DCM; Sigma-Aldrich; 270997). On the next day ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were typically seeded at a density of 1-3 × 105 cells/cm2 on dishes coated with poly-L-lysine (Sigma-Aldrich, P1524). Neuronal cultures were treated overnight in 1 μM tetrodotoxin (TTX ...
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bioRxiv - Molecular Biology 2022Quote: ... were washed in 1X PBS and co-cultured at a ratio of 5 parasites per macrophage (MOI 5:1) in RPMI 1640 medium without L-arginine (R1780, Sigma-Aldrich, USA) supplemented with 2% of inactivated FBS (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 5°6.7’ E) in September and October 2017 by filtering 2 L of seawater through PVDF membrane filters (Merck Millipore, Burlington, Massachusetts,USA), pore size ...
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bioRxiv - Neuroscience 2022Quote: ... mouse hippocampal neurons of both sexes were harvested at day 17 of embryonic development (E17) and maintained in dishes coated with poly-L-lysine (Sigma-Aldrich, Canada) for 7 days in Neurobasal medium (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... Groundtissue was mixed with 500 µL extraction buffer (50mM Tris, pH 7.5, 150 mM NaCl, 5mM EGTA, 5mM EDTA 0.3% β-mercaptoethanol, 1% Sigma Plant Protease Inhibitor) and homogenize immediately by Omnitip homogenizer for 10s on ice ...
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bioRxiv - Synthetic Biology 2020Quote: ... The strains were precultured on Yeast Nitrogen Base (YNB) medium without amino acids and with 5 g/L ammonium sulfate (Sigma-Aldrich Y0626) and 10 g/L glucose ...
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bioRxiv - Neuroscience 2019Quote: ... A 10 µL microsyringe with stainless steel 33G beveled needle was filled with a 20 mM solution of kainic acid (KA; Sigma-Aldrich, France) in 0.9% sterile NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... or SD minimal medium or SD-Complete medium (60) containing leucine at 170 mg/l and all other amino acids (Sigma Aldrich, USA) at 85 mg/l.
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bioRxiv - Microbiology 2019Quote: ... The next morning medium from each flask was replaced with one of the test media [modified Leibovitz’s L-15 (Sigma Aldrich, NSW, Australia), Schneider’s (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2019Quote: To measure sensitivity to perturbation of dopamine signaling we employed the startle response assay after pharmacological treatment with cocaine (Cocaine, COC-156-FB, Lipomed AG, Austria) or 3IY (3-Iodo-L-tyrosine, I8250, Sigma Aldrich, USA). Flies were reared for two generations in the high-temperature regime common garden experiment (Supplementary Figure S1) ...
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bioRxiv - Immunology 2020Quote: ... collected tissues were minced and incubated for 1 hour at 37°C in an agitated incubator (60 rpm) in HEPES buffer (pH 7.4) containing 0.5 g/L collagenase type I from Clostridium histolyticum (Sigma-Aldrich, Zwijndrecht, The Netherlands) and 2% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... The non-adherent cardiomyocytes were seeded in complete Cardiomyocyte plating medium supplemented with 10% fetal calf serum and antibiotics at a density of 4-5 × 105 cells/well on Poly-L-Lysine (Sigma P-7890) and Gelatin (Sigma G9891)-coated coverslips in 6-well plates ...
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bioRxiv - Molecular Biology 2019Quote: Immunoprecipitations were set up with 100 µl solubilised chromatin (roughly equivalent to 4 × 106 cells) and 3 µg of an anti-acetylated histone H3 antibody (Millipore, 06-599) in a total of 1 ml Native ChIP Incubation Buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: Secondary antibodies used are: Goat anti-mouse IgG (H+L) highly cross-adsorbed Alexa fluor plus 647 secondary antibody (Sigma-Aldrich, A32728), Goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... adding a drop of water to prevent the gel from drying (ibidi chambers and coverslips were poly-lysine coated by incubation with poly-l-lysine solution (0.1% w/v in water (P8920, Sigma-Aldrich, lot: 050M4339) for at least 45 min at RT shaking and dried with pressured air) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 10 μM L-ascorbic acid 2-phosphate (Wako Chemicals; Richmond, VA) and 10 mM β-glycerol phosphate (Millipore Sigma (St. Louis, MO). Triplicate wells were exposed to differentiation media without any agent (appropriate vehicle only-control) ...
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bioRxiv - Microbiology 2020Quote: ... N-acetyl-L-cysteine (NAC) supplemented medium were prepared at the final concentration of 1 mM (A9165, Sigma (Shaposhnikov et al. 2018)).
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 2 mM L-glutamine and 1% Antibiotic -Antimycotic (Life Tech, UK) with 20 ng/mL human epidermal growth factor (hEGF, Sigma-Aldrich, UK), 20 ng/mL human basic fibroblast growth factor (hbFGF ...
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bioRxiv - Pathology 2021Quote: ... submerged in serum-free culture medium consisting of low-glucose (1 g/l) Dulbecco’s Modified Eagle’s medium (DMEM; Sigma-Aldrich, MilliporeSigma, Burlington, MA) supplemented with 1% PSA ...
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bioRxiv - Cell Biology 2019Quote: ... obtained with cryomicrotome (Microm HM-560, Walldof, Germany) [17] have been collected on slides coated with poly-L-lysine (Sigma, Milan, Italy) and stored until use ...
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bioRxiv - Neuroscience 2021Quote: ... The passaged NSCs were used at P2 for differentiation into cortical neurons by plated them on poly-L-ornithine (20ug/ml) (Sigma, Cat # P4957) and laminin (5ug/ml ...
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bioRxiv - Biochemistry 2019Quote: ... 3T3-DCSIGN and 3T3-L-SIGN cells were incubated with 200 μg/mL of yeast mannan (Sigma-Aldrich, St. Louis, MO, USA) or 0.5 mM EDTA at 37 °C for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... All pipelines are connected to an oil bottle at one end that supplies the carrier oil (mineral oil with 10 g/l Span 80, Sigma Aldrich, Germany) for the entire system ...
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bioRxiv - Biochemistry 2020Quote: ... Heparin (20 μl of 50 mg mL−1) was reacted with 20 μl hydroxylamine-biotin in 40 μl 300 mM aniline (Sigma-Aldrich, UK) and 40 μl 200 mM acetate pH 6 for 48 h at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... AS were obtained from VMB and STR brain areas from mice at postnatal days P2-P4 and cultivated in DMEM (1 g/L glucose, Sigma Aldrich, D6046) supplemented with 10% FBS (Biowest ...
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bioRxiv - Neuroscience 2020Quote: ... Animals were treated daily in a volume of 10 mL/kg of body weight with a combination of L-Dopa (5-25 mg/kg; Sigma-Aldrich D1507) and the peripheral L-amino acid decarboxylase antagonist ...
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Physiological activation of the nephron central command drives endogenous kidney tissue regenerationbioRxiv - Physiology 2021Quote: ... WI) with or without ACE inhibitor treatment using enalapril (150 mg/L via drinking water, E6888 purchased from Sigma Aldrich Solutions, MO) with or without selective COX-2 inhibitor (SC58236 ...
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bioRxiv - Neuroscience 2021Quote: REPL-NG was synthesized by a two-step chemical reaction: the thiolation of ε-poly(L-lysine) (EPL, Zhengzhou Bainafo Bioengineering, China) by 2-iminothiolane (IT, Sigma. USA) and then formation of multiple disulfide bonds among thiols in thiolated EPL ...