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Citations for Millipore Sigma :
7651 - 7700 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Slices were blocked and permeabilized in blocking buffer (10% donkey serum [Jackson ImmunoResearch 017-000-121] in PBS with 0.3% Triton [Sigma T9284] and 100mM L-lysine [Sigma L5501]) for one hour at room temperature ...
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Deletion of adipocyte Sine Oculis Homeobox Homolog 1 prevents lipolysis and attenuates skin fibrosisbioRxiv - Molecular Biology 2024Quote: ... were thawed and to 70% confluency in 10ml of complete pre-adipocyte growth media composed of high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4mM L-glutamine (Sigma Aldrich, Cat# D5796) supplemented with 10% calf serum (Colorado Serum Company ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded at a density of 0.5 × 106 on 6-well plates precoated with 50 μg/mL poly-L-ornithine (Sigma #P-3655) and 1 µg/mL fibronectin in DMEM F12 with 1 % N-2 supplement (Invitrogen #17502048) ...
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bioRxiv - Systems Biology 2024Quote: ... pre-coated with Poly-L-ornithine hydrobromide (6 µg/mL in 1xPBS, Sigma-Aldrich, for 1 hour at 37°C, SigmaAldrich) followed by Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (1.2 mg/mL in 1xPBS for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... CGNs were seeded in 12-well plates with cover glass (C018001, Matsunami Glass, Osaka, Japan) previously coated with 15 μg/ml poly-L-ornithine (P2533, Sigma-Aldrich) at a density ranging from 0.5 to 1 × 106 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were plated at a density around 500,000 cells per 60-mm dish on poly-L-lysine and laminin (1 mg.mL-1, Sigma-Aldrich, #P2636, #11243217001) precoated glass coverslips (18 mm 1.5H ...
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bioRxiv - Neuroscience 2024Quote: ... Cell culture plates (Denville Scientific, Inc., Plainfield, NJ, USA) were coated overnight with poly-L-lysine (50 µg/mL; Sigma-Aldrich), supplemented with 7.5 µg/mL laminin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2024Quote: ... 5.1 and 5.2 were first pressurized at 500 mbar for chip priming with water infused with 0.085 g/l of Pluronic F108 (Sigma-Aldrich, USA) and was decreased to 0 mbar ...
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bioRxiv - Biochemistry 2024Quote: ... 20 μM ELAC2 and 20 μM mt-tRNA precursor in buffer D with 200 μM S-(5’-Adenosyl)-L-methionine (SAM) (Sigma-Aldrich). The RNA and ELAC2 contained a higher NaCl concentration ...
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bioRxiv - Neuroscience 2024Quote: ... These cells were then transferred to cell-culture plastic plates that had been previously treated with poly-L-lysine 10 μg/ml (Sigma-Merck) to enhance cellular adhesion and promote monolayer cultures ...
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bioRxiv - Developmental Biology 2024Quote: ... and pCMV-Sport6.ccdb-marcksl1.L were linearized with EcoRV followed by synthesis of anti-sense RNA probes using the digoxigenin RNA Labeling Kit (Sigma Aldrich) and SP6 polymerase ...
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bioRxiv - Systems Biology 2024Quote: The filtrate was lyophilized and suspended in 50 μL of water containing the reference compounds [200 μM each of trimesate (206-03641, Wako) and 3-aminopyrrolidine (404624, Sigma-Aldrich)] ...
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bioRxiv - Microbiology 2024Quote: ... The existing microbiota was depleted by broad-spectrum antibiotics in drinking water at concentrations of 1 g/l (ampicillin and metronidazole, Sigma-Aldrich) and 0.5 g/l (neomycin and vancomycin ...
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bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
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A non-invasive method to sample immune cells in the lower female genital tract using menstrual discsbioRxiv - Immunology 2024Quote: ... the cell pellet was resuspended in 1 mL freezing medium (50% FBS, 40% RPMI with L-glutamine, 10% DMSO (Millipore Sigma)) ...
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bioRxiv - Cell Biology 2020Quote: ... media was aspirated from confluent cells and replaced with a minimal volume of serum free DMEM-F12 supplemented with 50 μg/ul L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902). Cells were left overnight at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2020Quote: ... confluent cells were serum starved for 24 h in the presence of 50 μg/ul L-ascorbic acid-2-phosphate (Sigma Aldrich, A92902). At the time of assay ...
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bioRxiv - Microbiology 2021Quote: ... An aliquot of each dilution (100 ul) was plated onto Brain Heart Infusion (BHI) (Oxoid, UK) agar supplemented with mupirocin (50mg/l) (Sigma-Aldrich, UK), L-cysteine hydrochloride monohydrate (50mg/l ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The different concentrations of spinetoram were obtained by the dilution of the formulated insecticide (Exalt® 120g a.i./l) in distilled water with the addition of 0.1% (v/v) of the surfactant Triton X-100 (Sigma Aldrich Brasil Ltda). In each well ...
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bioRxiv - Developmental Biology 2021Quote: ... vehicle control and treated (n=3 per group) were incubated in the sepeate fish tank containing dissolved BrdU (25 μg/L) (Sigma-Aldrich, USA) for 24 hours before tissue collection ...
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bioRxiv - Molecular Biology 2022Quote: ... was induced in male C57BL/6 mice at 11–12 wk of age by a single injection of folic acid (240 mg/kg in vehicle, 0.15 mol/l NaHCO3, pH 7.4, Sigma-Aldrich, St. Louis, MO) (9 ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Neuroscience 2021Quote: ... The body weight of the stimulated animals was monitored to determine the appropriate volume of LPS from Escherichia coli (E.coli O127:B8, Sigma Aldrich L 3129). Animals were either injected with 2x 0.5 mg/kg LPS or 0.9% sodium chloride (NaCl ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... Colonies were picked and grown in 5 ml liquid SD-L-W medium and the prey vectors were isolated using the GenElute Plasmid Miniprep Kit (Sigma-Aldrich, USA) after cell wall disruption ...
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bioRxiv - Genomics 2022Quote: ... HEK293FT and NIH/3T3 cells were maintained at 37°C with 5% CO2 in DMEM with high glucose and stabilized L-glutamine (Caisson DML23) supplemented with 10% fetal bovine serum (Serum Plus II Sigma-Aldrich 14009C) and no antibiotics ...
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bioRxiv - Genomics 2019Quote: ... DMEM with high glucose and stabilized L-glutamine (Caisson DML23) supplemented with 10% fetal bovine serum (Serum Plus II Sigma-Aldrich 14009C) and no antibiotics.
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bioRxiv - Microbiology 2019Quote: ... Infant faces were isolated on RCM (Oxoid, Hampshire, UK) supplemented with mupirocin and L-cysteine (0.05 mg/mL each, Sigma-Aldrich, Dorset, UK). All Bifidobacterium and Lactobacillus strains were grown at 37°C in either RCM ...
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bioRxiv - Neuroscience 2019Quote: ... RtTA/Ngn2-positive iPS cells were plated as single cells at DIV0 onto nitric-acid treated coverslips coated with 50μg/mL poly-L-ornithine hydrobromide (PLO; Sigma-Aldrich #P3655-10MG) and 5 μg/mL human recombinant laminin 521 (BioLamina #LN521-02 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were cultured in phenol red-free DMEM supplemented with 2mM L-glutamine and 10% charcoal-dextran stripped fetal calf serum (Sigma-Aldrich, F6765), hereafter termed steroid-free media (SFM) ...
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bioRxiv - Microbiology 2019Quote: A single bacterial colony was inoculated in pre-reduced BHI broth (Oxoid, UK) supplemented with 1 g/L cysteine (Sigma-Aldrich, UK) and 5 g/L yeast extract and incubated at 37°C overnight ...
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bioRxiv - Microbiology 2019Quote: ... Serial sections were mounted on one slide coated with egg-white glycerin and on two slides with poly-L-lysine solution (Sigma-Aldrich, USA) for HE staining and fluorescence in situ hybridization (FISH) ...
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bioRxiv - Cancer Biology 2019Quote: 10 µL of cell suspensions that were retrieved from the microfluidic chip device as aforementioned were fixed with equal volume of 4% paraformaldehyde (PFA) and then dispensed on a poly-L-lysine coated glass slide (Sigma-Aldrich, USA). The cells were incubated at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... Netherlands) containing 200 mL three-fold diluted industrial wort supplemented with 0.4 mL L−1 Pluronic antifoam (Sigma-Aldrich, St. Louis, MO). Bottle caps were equipped with a 0.5 mm x 16 mm Microlance needle (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... the superficial glia directly adjacent to l-LNvs somas was locally digested with protease XIV solution (10 mg/ml, SIGMA-ALDRICH P5147) dissolved in external recording solution ...
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bioRxiv - Neuroscience 2021Quote: ... The arrays were coated with poly-L-lysine overnight at 4°C (500µg/mL in borate buffer; PLL) (Sigma-Aldrich, MO, USA) and the next day with laminin for 1h at RT (5µg/mL in distilled water ...
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bioRxiv - Neuroscience 2020Quote: ... to dimensions 2mm Ø x 0.5 mm depth and loaded with either PBS (control) or 10mg/mL L-α-Lysophosphatidylcholine (LPC, L4129, Sigma-Aldrich) and placed onto the exposed cortical surface ...
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bioRxiv - Biophysics 2021Quote: Wild type and p75NTR KO CGNs were trypsinized and plated at a density of 40 000 (for cell death assay) or 200 000 (for protein collection) cells per coverslip coated with poly-L-lysine (Sigma, Cat: P7280) in a 24-well (Starlab ...
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bioRxiv - Bioengineering 2021Quote: ... The yeasts were grown in yeast extract (1%, w/v) peptone (2%, w/v) glucose (2%, w/v) (YPD) media supplemented with 50mg/L ampicillin (Sigma-Aldrich, 69534). The strains were grown in 250 mL Erlenmeyer flasks containing 100 mL of YPD ...
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bioRxiv - Bioengineering 2021Quote: ... we added ProxUp and ECGM2 media mixed at 1:1 ratio and containing an additional 5.5 mmol/L of D-Glucose (Sigma Aldrich, Cat# G5767) to all the wells on days 5 ...
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bioRxiv - Bioengineering 2021Quote: ... DMEM with high glucose and stabilized L-glutamine (Caisson DML23) supplemented with 10% Serum Plus II fetal bovine serum (Sigma-Aldrich 14009C) and no antibiotics ...
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bioRxiv - Bioengineering 2020Quote: ... and 800 mg L-1 humic acids (CAS No. 1415-93-6) were prepared by adding in ultrapure water (Sigma Aldrich, UK).
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bioRxiv - Neuroscience 2021Quote: ... The medium was replaced every 2-3 days until day 10 when each NP was individually plated for allowing neurite outgrowth on surfaces previously coated with 100 μg/mL poly-L-ornithine (Sigma Aldrich, P3655) and 20 μg/mL laminin (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC-derived neuron progenitors were expanded using 6-well plates coated with 15 μg/ml Poly-L-Ornithine (Millipore Sigma Cat# P4957) and 10 μg/ml laminin (Invitrogen Cat# 230171015 ...
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bioRxiv - Neuroscience 2021Quote: ... In short: animals were initially perfused with 30 ml of phosphate-buffered saline (PBS) with heparin (100 mg/l, Sigma, Cat#H0878), followed by 30 ml of 4% (w/v ...
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bioRxiv - Plant Biology 2020Quote: ... Acid immersion was followed by a neutralisation treatment in a 8.4 g L-1 sodium bicarbonate (NaHCO3, Sigma-Aldrich, St Louis, USA) solution for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... 5×106 cells/ ml) in N-free medium were seeded on cover slips coated with poly-L-lysine (Sigma, Cat. No. P8920) for 5 minutes followed by fixation with 4% paraformaldehyde solution (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... and cultured in Complete media (base media + 50% WRN-conditioned media prepared from L cells expressing Wnt/R-spondin/Noggin as described (48)) with 10 μM Y27632 (ROCK inhibitor, Sigma-Aldrich Y0503) at 37 °C 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...