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Citations for Millipore Sigma :
7601 - 7650 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... bacterial culture obtained from overnight culture in LB broth (Luria Bertani broth, HIMEDIA, Cat# M1245) supplemented with 0.2 % L-Arabinose (Sigma, Cat# A81906) and 100μg/ml Ampicillin (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... A total of 400 mL of autoclaved water with Hoagland’s solution (1.6g/L, Catalog No. H2395-10L, Sigma, San Luis, Misuri) was added into the calcined clay and mixed thoroughly ...
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bioRxiv - Zoology 2023Quote: ... Healthy fish with a body weight of 100-500 g were euthanized by immersion in a tank containing 150 mg/L MS-222 (Sigma, USA) for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... was changed daily and animals over 4 days post fertilisation were euthanized by overdose of MS-222 (300 mg/l) or 15% 2-Phenoyethanol (77699 SIGMA-ALDRICH) at the end of experiments.
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bioRxiv - Cancer Biology 2023Quote: The following is a list of chemical compounds used in cell culture experiments: 1 mM N-acetyl-L-cysteine (A9615, Sigma-Aldrich), 100 μM Trolox (238813 ...
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bioRxiv - Neuroscience 2023Quote: ... hippocampi from postnatal mice (p0-p3) were surgically isolated and dissociated into single cells by trypsin (5 g/L, Sigma-Aldrich) digestion ...
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bioRxiv - Plant Biology 2023Quote: ... a 20 µL aliquot of AWF was lyophilized to dryness and resuspended in 10 µL of 30 mg/mL methoxyamine hydrochloride (Sigma-Aldrich) in pyridine (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: Cells were seeded the day before into 24-well plates containing sterile glass coverslips coated with poly-L-Lysine (Sigma-Aldrich). The seeding density per well was 150,000 cells for HEK293T and 50,000 cells for U2OS and Huh7 cells ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were cultured in Poly-L-Ornitine pre-coated flat bottom 12-well plate in a high glucose DMEM (Sigma, D7777) medium supplemented with 44 mM NaHCO3 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... 24-well glass-bottomed plates coated with Poly-L-ornithine/laminin (15 µg/mL Sigma Aldrich 10 µg/mL Sigma Aldrich), respectively ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of the diluted DNA was used as template in a qPCR reaction with SYBR Green JumpStart Taq ReadyMix (Sigma-Aldrich). Primer sequences targeting Cryptosporidium GAPDH were as follows ...
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bioRxiv - Microbiology 2023Quote: ... 50µL of NaCl were then added to the strip before being centrifuged (19,000 x g for 20 minutes, Sigma 3-16PK) to extract the tears ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cultures for genome editing were grown in yeast extract-peptone-dextrose (YPD) medium (50 g/L YPD Broth (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was gently disaggregated and neurons plated for 90 minutes with DMEM 10% HS medium in P6 well plates previously treated with 1 mg/ml poly-L-lysine (Sigma Aldrich) at a density of 300,000 cells/cm2 ...
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bioRxiv - Cancer Biology 2023Quote: Exponentially growing cells were seeded onto 10 mm glass coverslips previously treated with poly-L-lysine (Sigma-Aldrich, Catalog No. F8775). After 48 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... washed twice in 150 µL ice-cold PBSA (1X PBS with 1 g/L bovine serum albumin (Sigma-Aldrich, Cat.#A4503)) ...
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bioRxiv - Neuroscience 2023Quote: ... and single cells seeded and grown as adherent cultures in flasks coated with 100 μg/ml Poly-L-ornithine (Sigma-Aldrich) and 5 μg/ml mouse laminin in media composed of advanced DMEM/F12 ...
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bioRxiv - Neuroscience 2023Quote: ... The cortex or thalamus was dissected and treated with 0.05 % trypsin (Fujifilm-Wako) at 37℃ following mechanical dissociation to yield single cells and then plated onto poly-L-lysine (SIGMA, #P2336) coated tissue-culture plates (IWAKI ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was immediately removed and rapidly immersed in oxygenated (95% O2 – 5% CO2) ice-cold “cutting” solution containing (in mmol/L; all from Sigma, US): 87 NaCl (#S9888) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were seeded on coated plates (Poly-L-ornithine [0.001%; A-004-C; Sigma-Aldrich] and laminin [300ng/mL; L2020; Sigma-Aldrich]) in 2i+LIF culture medium (N2B27 Media ...
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bioRxiv - Immunology 2023Quote: ... were co-cultured in round-bottomed 96-well plates in DC medium (RPMI-1640, 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) the presence of indicated concentrations of OVA323-339 peptide (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 µg/mL, Sigma-Aldrich, St. Louis, MO) and cultivated in DMEM medium containing 10% FCS and 2 mM L-glutamine at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at a density of 1 million cells per 6-well plate (or 375k cells per 12-well plate) in poly-L-ornithine (Sigma P3655) -coated wells in Neuron Differentiation Media with 10 uM ROCK inhibitor ...
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bioRxiv - Genomics 2023Quote: ... 2-3 month-old adult individuals were anesthetized using tricaine at 225 mg/l (PharmaQ, tricaine) diluted in water with 450 mg/ml of sodium bicarbonate (Sigma, S5761) and a small piece of the caudal fin was biopsied for genomic DNA extraction ...
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bioRxiv - Genomics 2023Quote: ... Fish were euthanized using an overdose of tricaine at 300 mg/L (PharmaQ, tricaine) diluted in water with 600 mg/ml of sodium bicarbonate (Sigma, S5761). The body cavity was opened and ovaries were fixed in situ to preserve their structure ...
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bioRxiv - Microbiology 2023Quote: ... A 500 μL aliquot was collected every 30 s or 45 s and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Immunology 2023Quote: ... and 10-12 were treated with 100 µL of embryo water plus 1 µg/mL H2DCFDA and 400 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). The plates are covered with aluminum foil and incubated at 28°C for 2.5 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 24-well glass-bottomed plates coated with Poly-L-ornithine/laminin (15 µg/mL Sigma Aldrich 10 µg/mL Sigma Aldrich), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... Biopsies were subsequently microtome sectioned to a thickness of 3 µm and mounted onto poly-L-lysine coated Poly-Prep glass slides (Cat. No. P0425-72EA, Sigma Aldrich). After incubating the slides at 37 °C over night deparaffination was performed ...
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bioRxiv - Developmental Biology 2023Quote: ... Media culture was a mixture of 75% freshly prepared rat serum (filtered through a 0.2-mm filter) and 25% DMEM (containing 1 mg/ml D-glucose and pyruvate, without phenol red and L-glutamine-D5921 Sigma-Aldrich), supplemented with 1× glutamax ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... the medium was replaced with 2 mL of prewarmed full growth medium containing 2 µg/mL of puromycin and 25 mM of sodium L-ascorbate (Sigma-Aldrich). Puromycin selection was performed for 2 days ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Greiner Bio-One CELLSTAR Polystyrene 6-well Cell Culture Multiwell Plates coated first with Poly-L-ornithine hydrobromide (6 μg/mL in 1xPBS (Sigma-Aldrich), 1 h at 37℃ ...
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bioRxiv - Biochemistry 2023Quote: ... 15N isotopically labelled protein sample was made from a 1 L culture containing M9 minimal media salts supplemented with 3 g of 13C-glucose (Sigma-Aldrich), 1 g of 15N ammonium chloride (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... was stored at −30 °C and used at 1 g/ L in 10 mM of calcium chloride (CaCl2) solution (Sigma-Aldrich). For the crosslinker ...
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bioRxiv - Bioengineering 2023Quote: ... substrates were placed in the 6-well plates and they were coated with poly-l-lysine (PLL, Sigma-Aldrich, MO, USA) solution prepared in ddH2O ...
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bioRxiv - Neuroscience 2023Quote: ... and seeded at approximately 1 million cells/cm2 onto assay plates coated with poly-L-ornithine 44 µg/mL (Sigma P3655) and fibronectin 1 µg/mL (Sigma F1141 ...
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Dual inhibition of coronavirus Mpro and PLpro enzymes by phenothiazines and their antiviral activitybioRxiv - Microbiology 2023Quote: To visualize replication centers, 293T cells were seeded on #1.5 coverslips (Zeiss, 411025-0003-000) coated with poly-L-lysine (Sigma Aldrich, P2658) and infected the following day with HCoV-OC43 at an MOI of 1 ...
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bioRxiv - Microbiology 2023Quote: ... and murepavadin (from 0.06 to 128 mg/L) were determined by the standard microdilution method in MHB using titrated powders of colistin sulfate (Sigma-Aldrich) and murepavadin (ProbeChem ...
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bioRxiv - Neuroscience 2023Quote: ... the neurons were plated in 4-well plates coated with poly-L-lysine (1 mg/ml) (Cat. #: P2636-500MG, Sigma-Aldrich) and homemade laminin (Chauvet et al. ...
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bioRxiv - Neuroscience 2024Quote: ... 12μm-thick cryosections were converted onto RNAse-free polyethylene terephthalate membrane (PET) slides (Zeiss 415190-9051-000) after these had been coated with poly-L-Lysine (Sigma Aldrich).
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bioRxiv - Bioengineering 2024Quote: The sunitinib solution was prepared at a concentration of 20 mg/mL by dissolving 200 mg of sunitinib L-malate (Sigma Aldrich) in 1 mL of dimethyl sulfoxide and 9 mL of corn oil ...
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bioRxiv - Neuroscience 2024Quote: ... cells were dissociated using Accutase and plated in dishes coated with poly-L-ornithine (PLO; 0.1 mg/ml; Sigma, P3655-10MG). Cells were plated in neuronal maturation media (BrainPhys medium (STEMCELL Technologies ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 μL of these turbid cultures was used as starter cultures into 150 μL BHICR with three concentrations of βHB (Sigma 166898) - 0 mM ...
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bioRxiv - Cell Biology 2024Quote: ... Isolated islets were transferred to non-adherent dishes containing RPMI-1640 medium with 2 mM L-glutamine and 11 mM D-glucose supplemented with 10% FBS and 1% penicillin/streptomycin (p/s) (P4458, Sigma Aldrich) in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were seeded at a density of 30000 cells/well for regular experiments or 125,000 cells/well for transfection experiments in 4-well chamber slides (Lab-Tek II, 155382) coated with poly-L-lysine (20 µg/mL, Sigma, P1399) and laminin (2 µg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... 20 μM ELAC2 and 20 μM mt-tRNA precursor in buffer D with 200 μM S-(5’-Adenosyl)-L-methionine (SAM) (Sigma-Aldrich). The RNA and ELAC2 contained a higher NaCl concentration ...
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bioRxiv - Genomics 2024Quote: ... which is Dulbecco’s Modified Eagle Medium (DMEM) with high glucose and stabilized L-glutamine (Caisson DML23) supplemented with 10% fetal bovine serum (Sigma 14009C). HAP1 cells were cultured in I10 medium ...
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bioRxiv - Neuroscience 2024Quote: ... CGNs were seeded in 12-well plates with cover glass (C018001, Matsunami Glass, Osaka, Japan) previously coated with 15 μg/ml poly-L-ornithine (P2533, Sigma-Aldrich) at a density ranging from 0.5 to 1 × 106 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... Dissociated neurons were plated at a density around 500,000 cells per 60-mm dish on poly-L-lysine and laminin (1 mg.mL-1, Sigma-Aldrich, #P2636, #11243217001) precoated glass coverslips (18 mm 1.5H ...
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bioRxiv - Bioengineering 2024Quote: ... 5.1 and 5.2 were first pressurized at 500 mbar for chip priming with water infused with 0.085 g/l of Pluronic F108 (Sigma-Aldrich, USA) and was decreased to 0 mbar ...