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Citations for Millipore Sigma :
7901 - 7950 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... technique was used to transiently co-transfect the cells in a 15.6 mm well on a 24-well plate coated with poly-L-lysine and collagen (Sigma-Aldrich, Prague, Czech Republic) with 200 ng of GFP plasmid (TaKaRa ...
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bioRxiv - Neuroscience 2020Quote: ... Tadpoles were exposed to 4’-OH-PPF ± 5 nM T3 (3,3′,5-triiodo-L-thyronine sodium salt (T3) (Sigma - CAS 55-06-1)).
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bioRxiv - Neuroscience 2020Quote: ... for the dopaminergic-neuron plating, we used poly-L-ornithine (PLO, 20 μL, 0.05 mg/mL) solution (A-004-C, Sigma-Aldrich, St. Louis, USA) for 2 hours in a 5% CO2 cell culture incubator at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... bonded channels were cooled to room temperature and then treated with a 1:10 dilution of poly-L-lysine (Sigma catalog no. P8920) in Milli-Q water ...
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bioRxiv - Developmental Biology 2021Quote: ... and immersed in Leibovitz L-15 media supplemented with 0.4 mg/ml of the anesthetic MS-222 (Sigma Aldrich, cat no. A5040-25g). All Atomic Force Microscopy (AFM ...
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bioRxiv - Neuroscience 2021Quote: ... attached to a 40x objective giving a 2 mm diameter illumination was positioned 1.5 mm lateral from Bregma and 0.2 mL of Rose Bengal solution (Sigma; 10g/L in saline, i.p.) was administered ...
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bioRxiv - Bioengineering 2020Quote: Coverslips attached to 8 well Labtek chambers were incubated in Poly-L-Lysine (PLL) at 0.01% W/V (Sigma Aldrich, St. Louis, MO) for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... and plated out onto uracil-free (Ura-) selection medium (1◻g◻l−1 Yeast Synthetic Drop-out Medium Supplements without uracil (Sigma-Aldrich), 6.7◻g◻l−1 Yeast Nitrogen Base with Amino Acids (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 150 mM NaCl, 1 mM MgCl2, 1mM EDTA, 1% Triton X-100 (Boehringer Mannheim, 789704), 1mM DTT (L-Dithiothreitol, C4H10O2S2) (Sigma Aldrich, D0632-5G), protease inhibitor ...
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bioRxiv - Neuroscience 2022Quote: ... 150mM NaCl,1 mM MgCl2, 1mM EDTA, 1% triton X-100 (Boehringer Mannheim, 789704), 1mM L-Dithiothreitol (DTT, C4H10O2S2) (Sigma Aldrich, D0632-5G), protease inhibitor (Halt Protease inhibitor Cocktail EDTA-free 100x (Roche ...
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bioRxiv - Genetics 2022Quote: ... supplemented with 10 mM (low glucose) or 30 mM (high glucose) from a D-glucose stock solution (Sigma # G8644, 100 g/L stock). DMEM base medium contains more total nutrients than MEM—approximately 2-fold higher levels of the 13 essential amino acids ...
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bioRxiv - Physiology 2022Quote: ... bats were fed ad libitum with mango nectar (> 95% of the composition in the form of simple sugars - Serigy®) supplemented with 4 mg/l of hydrolyzed casein (Sigma Aldrich®). A known amount of nectar was placed in a feeding device at 06:00 pm and was weighed at 08:00 am to measure food intake.
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bioRxiv - Plant Biology 2022Quote: ... 0.5 mM luminol probe 8-amino-5-chloro-7-phenyl-pyrido[3,4-d] pyridazine-1,4(2H,3H) dione (L-012) (Wako Chemicals, Richmond, Virginia, USA) and 20 μg/ml horseradish peroxidase (Sigma, Saint Louis, Missouri, USA). ROS production was measured by a luminol-based assay 74 over 60 min with integration at 750 ms.
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bioRxiv - Microbiology 2022Quote: ... Of this 200 μL and left to form a biofilm in 48 wells plates (Corning® Costar® TC-Treated Multiple Well Plates from Sigma-Aldrich) or chamber slides (Lab-Tek® Chamber SlideTM System ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated on glass coverslips (Carolina Biological Supply, Burlington, NC) coated with poly-l-lysine (0.2 mg/mL: Sigma-Aldrich, St. Louis, MO) in 12-well plates (Greiner Bio-One ...
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bioRxiv - Developmental Biology 2024Quote: ... Two drops (5μl and 0.3μl) of premixed 3x Tribolium saline + Schneider’s solution (1:1) supplemented with 100 µmol/l of amaranth (Sigma-Aldrich, St Louis, MO, USA) were dropped under paraffin oil and their diameter measured ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 μg ml-1, Sigma-Aldrich, St. Louis, MO, USA) and cultivated in DMEM medium (PAA Laboratories GmbH ...
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bioRxiv - Plant Biology 2022Quote: ... we stirred 75 g of each field soil into 1 L of sterile MES monohydrate (2.5 mM; Sigma Aldrich, St. Louis, MO, USA). We allowed the suspensions to settle for 30 minutes and then vacuum-filtered them through sterile filter paper with 11-µm pores ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 200μl media including 10 μL of MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide, 5mg/mL) (Sigma-Aldrich, U.S., Cat. No. M2272) per well for 4h at 37°C (Ayazoglu Demir et al. ...
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bioRxiv - Microbiology 2024Quote: ... and influenza A/California/7/2009 H1N1pdm09 virus were amplified on MDCK α2-6 in MEM supplemented with 1% HEPES and 1 μg/ml trypsin treated with N-tosyl-L-phenylalanine chloromethyl ketone (Sigma, Oakville, ON, Canada). Viral titers were determined by plaque assays ...
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bioRxiv - Bioengineering 2024Quote: Confocal microscopy images were taken for endometrial cancer cell lines seeded on 0.01% poly(L-lysine)-coated cover slips (Sigma Aldrich, St. Louis, MO). Cells were incubated for 24 hours prior to fixation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with secondary antibodies at 4°C for 2 days in CF™ 633 Anti-Rabbit IgG H+L 1:1000 (Sigma-Aldrich; SAB4600132), Alexa Fluor® 488 AffiniPure Donkey Anti-Goat IgG H+L 1:800 (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were washed twice in PBST and once in PBS and mounted on glass slides coated twice with poly-L-lysine (Sigma-Aldrich, #P8920-100ML). The mounted tissue was imaged using a Zeiss LSM 900 confocal microscope using a 20x objective (NA 0.8) ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) containing 4500 mg/L glucose (Sigma-Aldrich, St. Louis, MO, USA, Cat# D5796) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... by dual-SMAD signaling inhibition and ventral neural patterning as previously described.129 Banked MNPCs were thawed onto dishes coated with 10 µg/ml Poly-L-ornithine (PLO) (Sigma-Aldrich; Cat#P3655) and 5 µg/ml laminin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... purified CAPS-GST protein was mixed with increasing concentrations of microtubules in Ca2+ Binding-Buffer (80 mM PIPEs, 2 mM MgCl2, 50 uM CaCl2, 1 mg/ml BSA, 10 uM leupeptin (Sigma Aldrich L-2884), 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;) ...
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bioRxiv - Neuroscience 2024Quote: ... astrocytes were thawed and plated at 20,000 cells/well in MEM with 10% Horse Serum and 6.7% glucose onto 24 well plates (Nest Scientific, 70201) containing 25mm coverslips pre-coated with 1mg/ml of Poly-L-ornithine (Sigma-Aldrich, P4957-50ML) for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... 100 μl of serum was mixed with 100 μl of acetate buffer (0.2 mol/L, pH 5.0) containing 100 units of ß-glucuronidase/aryl sulfatase (Cat. No. 1041140002, Sigma-Aldrich, St. Louis, MO) and 50 μM of the internal standard 4-HBP (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were then resuspended in 50 µl of Nuclei Dilution Buffer (1X 10x Genomics Single Cell Multiome Nuclei Buffer, 1 mM DTT, 1 U/µl Sigma Protector RNase Inhibitor) and filtered with a 40 µm Flowmi cell strainer ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 μM DAPT (N-[N-(3, 5-difluorophenacetyl)-l-alanyl]-s-phenylglycinet-butyl ester) and 4 μM TAT-cre (Millipore, cat. no. SCR508) overnight.
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bioRxiv - Microbiology 2023Quote: ... plates were washed again as above and 100 μl of a goat anti-rabbit IgG (H + L) alkaline phosphatase conjugate (Sigma, St. Louis, MO) diluted 1:2,000 in PBS/1% NFM were added to each well and incubated for 1.5h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cells were cultured in osteogenic medium (α-MEM-complete, supplemented with 2 mM β- glycerophosphate (BGP) and 50 µg/ml L-ascorbic acid (both Sigma-Aldrich)) ...
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bioRxiv - Cell Biology 2023Quote: ... and GalNAc-T2-GFP expressing Hela cells were maintained in DMEM with 10% FBS plus or minus 0.4 g/L G418 (Sigma-Aldrich, St. Louis, MO). GalNAc-T2-GFP Hela cells were a generous gift from Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... were deparaffinized and then subjected to antigen retrieval for 10 minutes at 121 uC at 15 PSI in 2.94 g/L sodium citrate (pH 6; Sigma Aldrich, 61332-04-3). Sections were permeabilized in PBS with 0.4% Triton X-100 (PBT ...
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bioRxiv - Biochemistry 2023Quote: ... cells were labeled and maintained in SILAC DMEM medium that was supplemented with 1mM 13C6 N2-L-lysine (Cat#608041, Sigma- Aldrich, Shanghai, China), 10% dialyzed fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... cells were dissociated using Accutase and plated in dishes coated with poly-L-ornithine (PLO; 0.1 mg/ml; Sigma, cat. no. P3655-10MG). Cells were plated in neuronal maturation media (BrainPhys medium (STEMCELL Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... resuspended in 30 μL chloroform and 15 μL of this resuspension was loaded for separation on silica gel on TLC aluminum foils (Sigma- Aldrich, 60805-25EA) using a solvent system containing 80:20:1 petroleum ether/diethyl ether/acetic acid for separation of neutral lipids ...
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bioRxiv - Biophysics 2023Quote: ... They were then resuspended in 500 ml of M9 media supplemented with 100 mg of a desired 15N-labeled or 13C-labeled L-amino acid (Cambridge Isotope Labs, Inc. or Sigma-Aldrich, Inc.) as well as the remaining 19 unlabeled amino acids ...
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bioRxiv - Cell Biology 2023Quote: ... 1 ml of differentiation medium (10% FBS (Millipore, Cat.No. ES-009-B, 1X L-Glutamine (Thermo Fisher, Cat.No.25030081, 1X Non-Essential AA, Millipore, Cat.No. TMS-001-C), 0.15 mM 2-Mercaptoethanol (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: 250 μL of the suspension (2 × 10 5 cells/mL) was applied to the upper layer of a Transwell chamber (Millipore, Billerica, MA, USA), which was placed in a 24-well plate containing 800 μL of 10% FBS at the bottom ...
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bioRxiv - Physiology 2023Quote: ... the heart was removed and placed into a dish containing a Tyrode’s solution supplemented with 0.2 mmol/L CaCl2 and 5 mg/ml BSA (Sigma-Aldrich, St. Louis, Missouri, USA). Atria were discarded and ventricles were cut into small pieces ...
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bioRxiv - Pathology 2023Quote: ... The solvent (n=5) or an FXIa inhibitor (ONO-1600586, 3 µmol/L n=5) and mepacrine (Sigma-Aldrich; final concentration 5 µM) were added to the blood before perfusion ...
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bioRxiv - Neuroscience 2023Quote: ... cells were dissociated using Accutase and plated in dishes coated with poly-L-ornithine (PLO; 0.1 mg/ml; Sigma, cat. no. P3655-10MG). Cells were plated in neuronal maturation media (BrainPhys medium (STEMCELL Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Each suspension was pipetted (20 μl) and spread using a sterile spreader on agar plate media (LB) using single-use L-shape spreaders (Sigma, cat no. Z723193) and cultured at 30°C for 48 h ...
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bioRxiv - Plant Biology 2023Quote: ... 200 μL of the fungal suspensions was aliquoted in clear 96-well flat bottom polystyrene tissue-culture plates (Greiner CELLSTAR®, Sigma, Burlington, U.S.A). Plates were incubated at 28°C ...
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bioRxiv - Physiology 2023Quote: ... bats were fed with mango nectar (> 95% of the composition in the form of simple sugars - Serigy®) supplemented with 4 mg L−1 of hydrolyzed casein (Sigma Aldrich®). We used the approach adopted by Bozinovic et al ...
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bioRxiv - Cell Biology 2024Quote: ... S% C02 in DMEM supplemented with 40 μM BrdU/BrdC (ratio 3:l, BrdU: BS002, Sigma-Aldrich, BrdC: sc-284SSS, Santa Cruz Biotech). Degradation of EGFP-AID-STAG2 was induced by the addition of S00 µM Inole-3-acetic acid (IAA ...
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bioRxiv - Genomics 2024Quote: ... iPSC-derived hNSCs were detached from hESC-qualified Geltrex using Accutase (STEMCELL catalog # 07920) and transferred to plates coated with Poly-L-ornithine (PLO)/laminin (Sigma Aldrich #P4957, #L2020) at low seeding density ...
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bioRxiv - Cell Biology 2024Quote: ... cells were dissociated using StemPro Accutase and reseeded in 8 well µ-Slides (IBDI #80826) pre-coated with 15 µg/mL poly-L-ornithine (Sigma #P-3655), 1 µg/mL laminin (Sigma #L4544) ...