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Citations for Millipore Sigma :
7801 - 7850 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The top ∼900 µL of cell suspension was drawn up into a 1 mL syringe and pushed through a reusable syringe filter (Millipore, Burlington, MA) containing 7 µm mesh (Component Supply ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (used for drug susceptibility assays) were cultured in plating medium (FreeStyle F17 supplemented with 10% FBS, 4 mM L-glutamine, 1x PenStrep (Sigma, Cat#P4458)) ...
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bioRxiv - Cell Biology 2023Quote: pHLARE expressing astrocytes were seeded at a density of 100,000 cells perwell onto poly-L-Lysine (Sigma catalog MILL-A-005-C) coated coverslips in individual wells of a 12 chamber plate ...
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bioRxiv - Microbiology 2023Quote: ... at 600 nm and 50μl of the culture was added to 5ml of LB alone or with different concentration of either bile salts (Sigma Aldrich, USA) (1% ...
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bioRxiv - Zoology 2023Quote: ... the animals were transferred to new vials in which the food was soaked with 50 µL of a 100 mmol L-1 all-trans-Retinal solution (R2500; Sigma-Aldrich, RRID:SCR_008988) and were kept in the dark for at least three days.
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bioRxiv - Cancer Biology 2023Quote: ... The treated cells were then transferred to cover slips pre-coated with a 0.1% Poly-L-lysine solution (Cat# P8920, Millipore Sigma, MO, USA) and incubated at 37°C with 5% CO2 for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS in fibronectin-coated cell culture flasks and seeded into plates coated with 20 μg/mL Poly-L-Lysine (Sigma-Aldrich, #9155) before experiments ...
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bioRxiv - Plant Biology 2023Quote: ... 600 μl aliquots of diluted chromatin were incubated with 7 μl of the rabbit anti-trimethyl-Histone H3 (Lys27) antibody (Millipore, 07-449) in a rotator at 4°C overnight ...
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bioRxiv - Genetics 2023Quote: ... the NPC differentiation media (RHBA with EGF, bFGF and ROCKi) was supplemented with 25 mg/ml L-ascorbic acid (Sigma Aldrich, A7506) and 1 mg/ml doxycycline (Tocris ...
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bioRxiv - Neuroscience 2023Quote: ... 500 μl papain inhibitor solution containing 5 mg/ml BSA (Carl Roth, 8076.4) and 5 mg/ml Trypsin inhibitor (Sigma-Aldrich/Roche, 10109878001) in PBS was added ...
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bioRxiv - Neuroscience 2023Quote: Expanded hydrogels were mounted on 2 x 3 x 0.15 mm glass coverslips (Claritex, prior to iSPIM imaging. Each coverslip was briefly dipped in Poly-L-lysine (Millipore Sigma, Rockville MD), air dried ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μl anti-FLAG M2-HRP was added and incubated for 90 min (prepared in 1% BSA, 1:10,000) (Sigma, Cat. no. A8592). Post antibody incubation ...
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bioRxiv - Bioengineering 2023Quote: ... with 10% (v/v) Fetal Calf Serum (FCS, First Link, UK) and 1% (v/v) 10 mM L-Glutamine (Sigma-Aldrich, UK). Trypsin with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Bioengineering 2023Quote: ... 90 μL of the remaining solution after ALP assay was mixed with 90 μL of digestion buffer (500 mM phosphate buffer, 5 mM L-cystein (Sigma-Aldrich, C1276), 5 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... and vitamin C at a concentration of 50 μg/mL [16] (L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate, Sigma Aldrich, A8960). For the study of the effect of the tissue 3D geometry ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was introduced by electroporation at stages 26-28 and RGC explants were taken at stages 35-36 and cultured for 19-24 h in 60% L-15 (Sigma-Aldrich, L1518) in water ...
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bioRxiv - Cancer Biology 2023Quote: ... and transcardially perfused with 10 mL PBS containing 1.5 mg D-luciferin potassium salt and 10 mg/L heparin sodium salt (Sigma Aldrich, Cat: H3393). Whole brains were immediately isolated ...
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bioRxiv - Biophysics 2022Quote: ... bovine α-lactalbumin (BLA, reference L-6010) and apo-myoglobin (apo-Mb, reference A8673-5X1VL) were purchased as powders from Sigma-Aldrich, USA ...
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bioRxiv - Bioengineering 2023Quote: ... or 90 min in 2 L of 0.02 M aqueous sodium carbonate (Na2CO3) solution (Catalog No. 451614, Sigma Aldrich, St. Louis, MO). Once degumming is complete ...
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bioRxiv - Immunology 2023Quote: ... Phoenix were grown to 60-80% confluence in DMEM in 10 cm culture dishes pre-coated 2 mL 0.01% poly-L-lysine (Sigma A-005-M) in 8 mL 0.1% gelatine (Sigma 1040700500 ...
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bioRxiv - Cell Biology 2023Quote: ... Pellets of cells expressing GFP-I3-01 oligomers were prepared analogously except the mitotic cells were induced by adding 10 μM S-trityl-L-cysteine (STLC; Sigma-Aldrich, 164739) for 14 hrs.
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bioRxiv - Cell Biology 2023Quote: Zoospores were adhered for 5 min to plasma cleaned 96-well plates (MatriPlate, Cat. No. MGB096-1-2-LG-L) coated with 0.5 mg/mL Concanavalin A (Sigma, Cat. No. C2010). We fixed the cells in 4% PFA and 50 mM sodium cacodylate ...
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bioRxiv - Neuroscience 2023Quote: ... Chemically defined medium (CDM) editing plates supplemented with 10 ng/ml BDNF and 10 ng/ml GDNF on poly-L-ornithine (Sigma-Aldrich, #P4957) and laminin (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... containing 12.5µL of assay buffer (125mM KCl, 20mM HEPES, 2mM MgCl2, 2mM KH2PO4, pH =7.4) supplemented with 40mM succinate (Sigma-Aldrich # S3674-100G), 40mM malate (Sigma-Aldrich #240176-50G) ...
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bioRxiv - Cancer Biology 2024Quote: ... For some experiments the PBS was adjusted to a final concentration of 6 mg/ml of poly-L-glutamate (Sigma- Aldrich, P4761). Plasmids were stored at 4°C for short-term and -20°C for long-term storage ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The organoids were then re-suspended in growth factor conditioned media and plated onto 0.01% Poly-L-Lysine (Sigma-Aldrich, cat # P4707) coated 96-well plates (Corning cat # 3603) ...
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bioRxiv - Molecular Biology 2023Quote: ... the pellets were resuspended in 200 μl of digestion buffer (1 % SDS, 100 mM NaCl, 50 mM Tris-HCl, 10 mM EDTA and 50U Lyticase (Sigma-Aldrich, L4025)) and incubated at 37ºC ...
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Understanding Campylobacter coli isolates from the Vietnamese meat production network; a pilot studybioRxiv - Microbiology 2023Quote: ... supplemented with 2.5% defibrinated horse blood (Oxoid, UK) along with 20 mg/L β- Nicotinamide adenine dinucleotide sodium salt (Sigma Aldrich, UK), at 120 revolutions per minute (RPM) ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL was spotted onto a clean glass slide and fixed with 4% paraformaldehyde (PFA) and Fluoromount™ Aqueous Mounting Medium (Sigma, F4680) before a cover slip was added ...
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bioRxiv - Neuroscience 2023Quote: ... Inserts were then placed into culture plate wells containing Neurobasal-A medium supplemented with 25 µM L-Glutamic acid (Sigma-Aldrich, G8415), 2 mM Glutamax (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... and were used at the indicated final concentrations in M2 medium: STLC 1 µM or 4 µM (S-Trityl-L-cystein, Sigma-Aldrich, 164739), Nocodazole 0,4 µM (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... supplemented with 42 g/L of carbohydrates in the drinking water (55% fructose, 45% sucrose; sourced from Sigma-Aldrich, St. Louis, MO)53 ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL of freshly dissolved histones was added to a premixed solution containing 3 μL of 10 mM Cy5-azide (Sigma-Aldrich, 777323), 10 μL of a 3:7 mixture of 50 mM CuSO4 and 100 mM THPTA ...
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bioRxiv - Immunology 2023Quote: ... bone marrow from C57BL/6J mice was treated with ACK and cultured in DC medium (RPMI-1640 [Thermo Fisher Scientific], 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) with GM-CSF (20 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... Corresponding water samples were taken by filtering 2-L of tank water through 0.22-μm pore-size cellulose acetate membranes (Millipore Sigma, Burlington, MA). Samples were stored at −80°C until processing.
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bioRxiv - Synthetic Biology 2023Quote: ... incubated at 42 °C for 15 mins then plated on uracil dropout (6.8 g/L Yeast Nitrogen Base Without Amino acids (Sigma, Catalog number: Y0626), 2% glucose ...
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bioRxiv - Immunology 2023Quote: ... Raji cells were mixed at a 1:1 ratio with JK cells and cells were spun down together to promote conjugate formation and incubated for up to 15 minutes on poly-L-lysine-coated coverslips (Sigma Aldrich, USA).
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bioRxiv - Systems Biology 2023Quote: ... and resuspended in 40 µl kinase buffer containing 300 µM ATP with 6 µCi γ-32P-ATP (Hartmann analytics) and 10 U bovine PKA (Sigma, 539576). For no PKA control ...
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bioRxiv - Cell Biology 2024Quote: ... and immediately digested in Cysteine-activated Papain solution (17U/ml Papain from Worthington, 20µg/ml DNase I from Sigma, 2mg/ml of L-Cysteine Hydrochloride from Sigma in Dissociation media) for 30 min at 37◦C ...
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bioRxiv - Plant Biology 2024Quote: ... Each final inoculum consisted of 10 mL of the resuspended pellet in 1 L 0.5X Murashige and Skoog basal salt mixture (MS; Sigma-Aldrich, Darmstadt, Germany). A control treatment was made by adding 10 mL sterile 1x PBS into 1 L 0.5X MS.
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bioRxiv - Bioengineering 2024Quote: Cells were incubated in poly-L-lysine–coated 96-well plates (2 × 104 cells/well) in Stemline II Hematopoietic Stem Cell Expansion medium (Sigma Aldrich; #50192) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: ... and seeded at a density of 2.0 x 104 cells/cm2 on glass coverslips coated with Laminin/Poly-L-Ornithine (Sigma and Merck-Millipore), or at a density of 5.0 x 104 cells/cm2 on Laminin-/Poly-L-Ornithine coated plates in StemDiff Forebrain Neuron Maturation medium (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: Human brain pericytes were cultured on a glass coverslip coated with 10 mg/mL of poly-L-lysine (Millipore Sigma, Cat# P8920). Cells were fixed and permeabilized with 4% paraformaldehyde for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... The OVSAHO cell line was maintained in RPMI 1640 medium containing L-glutamine and sodium bicarbonate (Sigma-Aldrich, St. Louis, MO, USA), complemented with the same supplements used in the previously described DMEM medium ...
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bioRxiv - Biochemistry 2024Quote: The activity of the enzyme was estimated by measuring the rate of hydrolysis of the fluorescent substrate L-leucine-7-amido-4-methyl coumarin (Leu-AMC, Sigma–Aldrich, L2145) followed at 460 nm (excitation at 380 nm ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% FBS in fibronectin-coated cell culture flasks and seeded into plates coated with 20 μg/mL Poly-L-Lysine (Sigma-Aldrich, #9155) before experiments.
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bioRxiv - Cell Biology 2024Quote: ... Carbohydrates were extracted by adding 44 µL of phenol and 2.5 mL of sulfuric acid to each sample or L-(-)-Glucose standards (Sigma-Aldrich cat. G5500) and allowed to incubate for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... and concentrated to approximately 150 µL by using an Amicon Ultra-15 centrifugal filter unit at 5000g for 30-60 min (NMWL 100 kDa, Sigma #Z740210-24EA). For large-scale gradient preps for intraparenchymal injection into mouse brain ...
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bioRxiv - Microbiology 2024Quote: ... cells were briefly pelleted and resuspended in R10 medium (RPMI supplemented with FBS, HEPES, L-glutamine, and penicillin-streptomycin) with 50 ng/mL of GMSF (Sigma Aldrich G5035) and were seeded into a 24-well plate at a density of 1E+06 cells/mL and incubated at 34°C ...
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bioRxiv - Microbiology 2024Quote: ... cells were briefly pelleted and resuspended in R10 medium (RPMI supplemented with FBS, HEPES, L-glutamine, and penicillin-streptomycin) with 50 ng/mL of GMSF (Sigma Aldrich G5035) and were seeded into a 24-well plate at a density of 1E+06 cells/mL and incubated at 34°C ...