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Citations for Millipore Sigma :
7551 - 7600 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... Yeast cultures for plasmid transformation and genome editing were grown in yeast extract-peptone-dextrose (YPD) medium (50 g/L YPD Broth (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2023Quote: ... cut into small explants as above using a tungsten needle and either cultured on poly-L-ornithine/laminin coated wells (both from Sigma-Aldrich) for growth cone morphology analysis or embedded in rat tail collagen (BD Bioscience ...
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bioRxiv - Neuroscience 2023Quote: ... for co-immunoprecipitation analysis and into 96-well cell culture plates (3×104 cells/well) coated with 0.01% poly-L-lysine (Sigma-Aldrich, #P9404) for ELISA ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then delipidated with clear unobstructed brain imaging cocktail and computational analysis (CUBIC)-L clearing solution (10 wt% N-buthyldiethanolamine [[#471240, Sigma-Aldrich, Germany] and 10 wt% Triton X-100 [Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature sensory neurons were dissociated and seeded as single cells onto plates coated with poly-L-ornithine hydrobromide (20 µg/mL, P3655, Sigma-Aldrich), laminin (10 µg/mL ...
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bioRxiv - Bioengineering 2022Quote: ... and cultured in adult brain slice culture medium containing 840 mg MEM Eagle medium with Hanks salts and 2mM L-glutamine (Sigma, M4642), 18 mg ascorbic acid (Sigma ...
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bioRxiv - Immunology 2023Quote: PBMCs were thawed at 37°C in complete RPMI-1640 media (supplemented with 10% FBS, L-glutamine, and Penicillin-Streptomycin-Amphotericin; RP10) containing benzonase (EMD Millipore). Cells were then counted and diluted in RP10 to a concentration of 75,000 live cells/mL ...
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bioRxiv - Developmental Biology 2023Quote: The individual effect of lactate on CAPA-IVM COC metabolism was studied trough supplementing the basal culture media with Calcium L-lactate hydrate (Sigma-Aldrich). An initial dose finding study was performed ...
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bioRxiv - Bioengineering 2023Quote: ... a solution of 1 mg/ml anionic crosslinker of poly-L-glutamic acid sodium salt (Sigma Aldrich, St. Louis, MO, USA) was prepared ...
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bioRxiv - Neuroscience 2023Quote: ... the SVZ of the medial-dorsal striatal ventricle wall was dissected out and mechanically dissociated in DMEM-high glucose (glucose 4500 mg/L, D6546, Sigma‒Aldrich) with 10% FBS (F7524 ...
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bioRxiv - Microbiology 2023Quote: ... l50,000 HEK293T cells were seeded on l3 mm diameter glass coverslips coated with poly-L-lysine (Sigma-Aldrich, Cat# P6282-5mg) in 24-well plates ...
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bioRxiv - Genetics 2023Quote: ... Osteogenic differentiation media consisted of BM-MSC complete growth media supplemented with 0.05 mg/mL L-ascorbic acid (Sigma-Aldrich A4403), 10 mM β-glycerophosphoric acid (ThermoFisher Scientific 410991000) ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... each well was coated overnight at 37°C with 1 mL of 100 μg/mL poly-L-ornithine hydrobromide (PLO, Sigma, P3655) prepared in filter sterilized 0.1 M borate buffer (Fisher Chemical ...
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bioRxiv - Cancer Biology 2023Quote: ... and 100 μl of 24 mM sodium arsenite (III) with 205 mM sodium chloride (NaCl) and 50 mM sodium hydroxide (NaOH, Sigma-Aldrich) were added to each well ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 µL of this solution was diluted in 220 µL of water and 25 µL Lugol’s iodine solution (L6146, Sigma, St. Louis). The reaction was incubated at room temperature for 10 minutes prior to absorbance measurements at 535 nm and 620 nm ...
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bioRxiv - Neuroscience 2023Quote: ... and neurons plated in NPM onto 24-well plates (5 x 104 cells/well) precoated with poly-L-lysine hydrobromide (Sigma P2636) (1 mg/mL for glass coverslips or 0.1 mg/mL for plasticware ...
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bioRxiv - Microbiology 2023Quote: ... or Luria-Bertani medium (LB, Carl Roth, Karlsruhe, Germany) liquid or solid medium amended with 15 g/l agar (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... 15N labelled protein was made from a 1 L culture containing M9 minimal media salts supplemented with 3 g of 13C-glucose (Sigma-Aldrich), 1 g of 15N ammonium chloride (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... were seeded according to FUJIFILM Cellular Dynamics user protocol onto the 96-well plates coated with poly-L-ornithine (P3655, Sigma-Aldrich) and laminin (L2020 ...
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bioRxiv - Neuroscience 2023Quote: ... the SCG neurons of postnatal day 1–2 NMRI strain mice or DRG neurons from E16 embryos were grown 6 DIV on poly-L-ornithine-laminin (P3655 and CC095, Sigma-Aldrich) coated dishes with 30 ng/ml of mouse NGF (G5141 ...
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bioRxiv - Neuroscience 2023Quote: ... 30µl of sample was transferred into a 96-well plate and added 100µl of Hexokinase enzyme reagent (HK) (Sigma-Aldrich, UK), and then mixed and incubated at room temperature for 15mins (Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 μL cell suspension was mixed with 150 μL DB+ containing 8 mM caffeine (Wako) and 20 μM latrunculin A (Sigma-Aldrich) and placed on a 35-mm glass bottom dish (12-mm glass in diameter ...
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bioRxiv - Neuroscience 2023Quote: ... hCOs were then transferred to individual wells of a 96 well plate and incubated with antibodies for 1 day for day 14 hCOs or 3 days for day 84 hCOs at 37° C in PTwH buffer (PBS; 0.5% Tween-20, Fisher, BP337; 10 mg/L Heparin, Sigma-Aldrich, H3393) while on an orbital shaker at 90 r.p.m ...
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bioRxiv - Microbiology 2023Quote: ... Carbohydrate sources and other components added to the medium for murine cultures were apple pectin (2 g/L, Sigma-Aldrich, USA), chicory inulin HP (1 g/L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... five individuals from each parent were sampled and euthanized with an overdose of Tricaine mesylate (MS -222; 500 mg/l; Sigma-Aldrich), measured for total body length and weight ...
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bioRxiv - Biochemistry 2023Quote: ... N-(3-Oxoocatnoyl)-L-HSL (>97%) (3-Oxo-C8) and N-(3-Oxododecanoyl)-L-HSL (>98%) (3-oxo-C12) were used as received from Sigma-Aldrich. N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95% ...
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bioRxiv - Cell Biology 2023Quote: ... in buffer Carbonate (7,13g/L NaHCO3 + 1,59 g/L Na2CO3 pH 9,5) was used to immobilize 96 well plaques (Nunc Immuno™ MicroWell™ 96 well solid plates, Sigma), o.n ...
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bioRxiv - Biochemistry 2022Quote: ... Around 10-20 µL of each sample were added to a cover slide (previously washed with distilled water and 70% ETOH and later treated with 10 µL of Poly-L-Lysine (#P4832, Sigma-Aldrich)) and air-dried ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of cells were sonicated for 5 s before being loaded into a CellASIC® ONIX2 Y04C microfluidic plate (Y04C, Millipore) connected to the ONIX2 (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 104 cells/well were seeded on a PLL coated (25 μg/ml Poly-L-lysine hydrobromide (Sigma-Aldrich: P1274)) 96 well plate ...
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bioRxiv - Cell Biology 2023Quote: Gold beads with a volume of 500 μl of stock solution (Gold nanoparticles, 80 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, 753661 Sigma-Aldrich®) were pelleted (RT ...
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bioRxiv - Cell Biology 2023Quote: S2naive and S2Xpress cells were seeded in an 8-chamber Nunc LabTek II that had previously been coated with Poly-L-Lysine (P4832, Sigma-Aldrich). Cells were then incubated for 24 h ...
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bioRxiv - Cell Biology 2023Quote: Aurora B-GFP expressing cells were grown in 40% confluency and synchronized in interphase by double Thymidine Block and in mitosis by using S-trityl-L-cysteine (STC; Sigma-Aldrich) (Uretmen Kagiali et al ...
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bioRxiv - Genomics 2023Quote: ... total cellular ERAP2 protein was enriched by immunoprecipitation and incubated with L-Arginine-7-amido-4-methylcoumarin hydrochloride (R-AMC, A2027, Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Vitamin K3 (menadione) (1 mL/L of a 1 mg/mL stock which was prepared in 100% ethanol; Sigma, Cat. # M5625), TYG Salts (40 mL/L ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM EGTA, 5 mM glucose, 10 mM MES, 5 mM MgCl2, 150 mM NaCl, 1 g/L streptomycin; Sigma Aldrich) at RT and fixed with 4% paraformaldehyde (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... spheroids were dissociated using Accutase and replated on PO/LM/FN-coated plates at 1×105/cm2 density in Neurobasal medium+B27+L-Glutamine+25 ng/ml GDNF+25 ng/ml BDNF+25 ng/ml NGF+200 μM ascorbic acid (Sigma, A8960)+0.2 mM dbcAMP (Sigma ...
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bioRxiv - Plant Biology 2023Quote: ... Cell density was adjusted to OD600 = 0.4 and 4 μL of the bacterial suspensions were spotted on R2A agar plates either containing 0.5% sodium carboxymethyl cellulose (CMC; Sigma-Aldrich, C5678), 0.05% Remazol Brilliant Blue-Xylan (RBB-Xylan ...
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bioRxiv - Biochemistry 2023Quote: ... Coverslips were then immersed in a clean petri dish with 50 μg/mL poly-L-Lysine (PLL; Cat. No. P1274; Sigma-Aldrich) and incubated overnight ...
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bioRxiv - Cancer Biology 2023Quote: Frozen vials of epithelial-enriched or stromal-enriched fractions were defrosted by gently diluting the material in 50 mL of cold Tissue Preparation Medium (TPM, RPMI 1640 + 25 mM HEPES and 2 mM L-glutamine (Sigma R5886), 5% foetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... then it was placed in complete medium (DMEM supplemented with 10% FBS, 1% penicillin plus streptomycin, 1% L-glutamine) with 1mM DTT (Sigma, # 10197777001) for 10 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: Precultures were grown in mineral media (Figure 3) or buffered SD media supplemented with 2000 µg/L thiamine (thiamine HCL, Sigma-Aldrich) (Figure 5 ...
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bioRxiv - Cell Biology 2023Quote: ... the liquid culture phase was collected as a starter culture and diluted 1:100 in fresh BSFB (final volume of 2 L) supplemented with 5 µg/mL vancomycin (Sigma-Aldrich), and incubated on a shaker platform in a humidified ...
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bioRxiv - Genomics 2023Quote: ... centrifuged and resuspended in 170 µl of magnetic-activated cell sorting (MACS) buffer (Phosphate-Buffered Saline solution (PBS) containing 5% Bovine Serum Albumin (BSA, Sigma #A9647), 1 mol/l of Ethylenediaminetetraacetic Acid Disodium (EDTA ...
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bioRxiv - Cell Biology 2023Quote: ... For imaging, cells were plated on 15 mm coverslips (Assistent/ Glaswarenfabrik, 41001115) coated with 1X Poly-L-Lysine (Sigma Aldrich, P2636), and the same treatment protocol was followed ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary neurospheres were dissociated into a single-cell suspension using Accutase (Nacalai Tesque) and seeded in poly-L-lysine (Sigma-Aldrich)-and Matrigel Matrix (Corning)-coated dishes and subsequently passaged every 3 days with fresh NPC medium ...
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bioRxiv - Neuroscience 2023Quote: ... and it was mixed with Evan’s blue dye (0.0551 g per 1 L of artificial plasma, #E2129-10G, Sigma Life Science, India). It was warmed to 37°C and oxygenated by 95% O2/5%CO2 gas bubbled through the solution ...
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bioRxiv - Molecular Biology 2023Quote: ... Gold fiducials (6 nm; BSA tracer; Aurion, Wageningen, Netherlands) were buffer exchanged into 40 g L-1 sea salts (Sigma Aldrich) using centrifugal ultrafiltration unit (100 kDa MWCO ...
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bioRxiv - Molecular Biology 2023Quote: ... Excess stain was removed by dialysis at a 1:1000 ratio against aqueous sea salt solution (40 g L-1, Sigma Aldrich) using dialysis sleeves (14 kDa MWCO ...
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bioRxiv - Neuroscience 2023Quote: ... Equal volumes of cell suspension were plated onto the centre of the 96-well plates coated with poly-L-ornithine (P- 8638, Sigma-Aldrich). The cells were grown in DMEM/F12 medium (21331–020 ...