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Citations for Millipore Sigma :
7851 - 7900 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 10 µm sections were obtained in a Leica CM1850 cryostat and mounted in poly-L-lysine-coated slides (300 μg/mL, Sigma Aldrich, P2636).
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bioRxiv - Neuroscience 2024Quote: ... and concentrated to approximately 150 µL by using an Amicon Ultra-15 centrifugal filter unit at 5000g for 30-60 min (NMWL 100 kDa, Sigma #Z740210-24EA). For large-scale gradient preps for intraparenchymal injection into mouse brain ...
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bioRxiv - Neuroscience 2024Quote: Human brain pericytes were cultured on a glass coverslip coated with 10 mg/mL of poly-L-lysine (Millipore Sigma, Cat# P8920). Cells were fixed and permeabilized with 4% paraformaldehyde for 15 min ...
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bioRxiv - Microbiology 2024Quote: ... 130 µl of a phage-bacteria mixture was combined with 130 µl of medium and approximately 15 µl of chloroform (Sigma Aldrich, 372978) in each well of a 96-well deep well plate ...
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bioRxiv - Plant Biology 2024Quote: ... and combined with 0.2 mL of a 3 mg L-1 TAG C15:0 solution (Tripentadecanoin, reference T4257, Sigma-Aldrich™, USA) in anhydrous hexane 95 % (296090 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 25 nM or 50 nM siRNAs targeting specific genes: CASP3 (Qiagen, 1027417, SI02654603; Horizon Discovery, L-004307-00-0005; Sigma-Aldrich, SASI_Hs01_00139105), CASP7 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were captured on pre-labeled 1×3 mm glass slides coated with 10% poly-L-lysine (Sigma Aldrich, St. Louis, MO, USA) and maintained at 37° during sectioning ...
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bioRxiv - Cell Biology 2020Quote: ... 10 µm sections were transferred to high precision 1.5H coverslips (Marienfeld, Germany) previously incubated with Poly-L-lysine solution (P-4832, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Developmental Biology 2021Quote: ... 2mM L-Glutamate, Gibco; 1% v/v BSA, Sigma; ITS liquid media supplement, Sigma; 0.9% v/v glucose, Sigma; 0.1mg/ml Primocin, InvivoGen) as previously described (Baptista et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... The FahPM/PM mice were kept on water supplemented with 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. #: PHR1731-1G). Mice with more than 20% weight loss were humanely euthanized according to IACUC guidelines ...
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bioRxiv - Microbiology 2020Quote: ... 13C3-L-lactate (or 25 μl each when 13Cu-glucose and 13C3-D/L-lactate were combined) for a further 24 hours (all sourced from Sigma Aldrich, Gillingham, UK). Six separate incubation were performed for each species ...
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bioRxiv - Microbiology 2020Quote: ... The springs and the jar lids were cleaned (60°C, 1 h) in a 100 g/L sodium persulfate solution (Na2S2O8, Sigma-Aldrich, Buchs, Switzerland), then rinsed with nanopure water ...
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bioRxiv - Microbiology 2019Quote: ... The clear supernatant was adjusted to pH 2-3 and spiked with 2-ethylbutyric acid as the internal standard (IS) at a final concentration of 1 mmol/L (Sigma Aldrich, Lyon, France). The solution was centrifuged again before injection ...
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bioRxiv - Microbiology 2019Quote: ... 300μl of lysis buffer with protease, 100μl of Zirconium beads (Stratech, Ely, UK) and 300μl of Phenol (Sigma-Aldrich, St Louis, MO, USA) was added to the pellet and the sample was disrupted twice for 3 minutes in a tissue homogenizer (Bertin Technologies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and incubated for 30 min with 10 µM 6-chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) and 1mg/L Hoechst 33342 dye (Sigma Aldrich, United States) diluted in media ...
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bioRxiv - Genomics 2020Quote: ... microglia differentiation media was exchanged with HEK293T media (DMEM + 10% heat-inactivated FBS + final 2mM L-Glutamine) and supplemented with 100 hg/ml lipopolysaccharide (LPS, Sigma Aldrich L4391-1MG) or PBS ...
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bioRxiv - Bioengineering 2021Quote: ... 18 mm coverslips were coated with poly-d-lysine (PDL; 10 g/L, minimum 2 hours, P6407, Sigma-Aldrich, St. Louis, Missouri, USA), and HEK293 cells were seeded at 150K ...
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bioRxiv - Neuroscience 2019Quote: 5 day post fertilization larvae were anesthetized in a 200 ng/µ L solution of MS-222 and embedded in 1.2% low melting-point agarose (Sigma-Aldrich, A0701-100G). Agarose was removed from around the eyes to allow for undamped eye movements ...
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bioRxiv - Cancer Biology 2021Quote: ... were grown in Dulbecco’s 90% Dulbecco’s MEM (4.5 g/L glucose) with penicillin– streptomycin (100 U/ml) and 10% fetal bovine serum (Sigma-Aldrich, St Louis, MO, USA) in 5% CO2 cell culture incubator ...
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bioRxiv - Cell Biology 2021Quote: ... Eggs obtained by natural spawning were bleached and raised at 28°C in Volvic source water supplemented with 280 μg/L of methylene blue (Sigma Aldrich, Cat#: M4159). N-Phenylthiourea (PTU ...
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bioRxiv - Biochemistry 2021Quote: ... were combined and concentrated to approximately 150 µL in centrifugal filter units with a 3 kDa molecular mass cutoff (Amicon ultra centrifugal filters, 0.5 ml, Millipore, centrifugation at 14,000 g).
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bioRxiv - Bioengineering 2021Quote: ... Five grams of silk cocoons were degummed in 2 L of boiling 0.02 M sodium carbonate (Catalog No. 451614, Sigma Aldrich, St. Louis, MO) for 30 minutes in order to isolate pure silk fibroin protein ...
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bioRxiv - Microbiology 2020Quote: ... bonded channels were cooled to room temperature and then treated with a 1:10 dilution of poly-L-lysine (Sigma catalog no. P8920) in milliQ water ...
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bioRxiv - Systems Biology 2021Quote: ... 250 µl of lysis buffer was added to the tissue along with 5 µl of Protease inhibitor cocktail (50X stock, Sigma-Catalogue no. 11873580001) and incubated on ice for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 mg/mL of lyophilized powder or controls in Millipore water were added to 100 mg/L indigo carmine (Sigma-Aldrich catalog 131164) and incubated at RT ...
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bioRxiv - Plant Biology 2020Quote: ... 20 μL of (GlcNAc)6 was added in a final concentration of 10 μM to trigger ROS production in the absence or presence of 100 μL of effector protein in a final concentration of 50 μM in measuring solution containing 100 μM L-012 substrate (FUJIFILM, Neuss, Germany) and 40 μg/mL horseradish peroxidase (Sigma-Aldrich, Missouri, USA). Chemiluminescence measurements were taken every minute over 30 min in a CLARIOstar microplate reader (BMG LABTECH ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Systems Biology 2020Quote: ... on day 0 with a 50 µl volume of Escherichia coli O111:B4 LPS purified by ion exchange and with <1% protein and <1% RNA (Sigma Aldrich; catalog L3024), which was diluted in sterile ...
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bioRxiv - Neuroscience 2021Quote: On day 3 pre-neuron cells were replated onto dishes coated with freshly made poly-L-ornithine (PLO; 0.1 mg/ml; Sigma, Cat. No. P3655-10MG), either 96-well plates (50,000 per well) ...
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bioRxiv - Genomics 2021Quote: ... The female was anaesthetized for 5 min in a solution of MS 222 (tricaine methane sulfonate 0.18 g L-1; Sigma - Aldrich Co, Missouri, USA). Blood was then sampled from the caudal artery using a 23-gauge needle in a 1ml TB syringe (Becton ...
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bioRxiv - Bioengineering 2021Quote: ... Coverslips attached to 8 well Labtek chambers were incubated in Poly-L-Lysine (PLL) at 0.01% W/V (Sigma Aldrich, St. Louis, MO) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... The inoculum was prepared at 1 × 108 cells/mL in water supplemented with 2.5 g/L sand (Cat: S5631; Sigma-Aldrich Canada, Oakville, ON). Inoculation was conducted with a spray bottle 10 cm away from the plants until run-off ...
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bioRxiv - Molecular Biology 2021Quote: ... the antibody – lysate mixture was incubated at 4°C with rotation for 1 hr prior to the addition of protein A/G agarose beads (Millipore, 50 L/IP) and continued overnight incubation ...
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bioRxiv - Biochemistry 2022Quote: ... cells in a 55 - cm2 dish were washed in PBS and then lysed for 15 min at 4 C in 500 L of Cel Lytic M buffer (Sigma, St. Louis, MO). After centrifugation ...
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bioRxiv - Neuroscience 2022Quote: Fish treated with dexamethasone to inhibit inflammation were exposed in system water to 15 mg/L dexamethasone (D1756; Sigma-Aldrich, Burlington, MA, USA) diluted in 0.1% MetOH [21] ...
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bioRxiv - Bioengineering 2022Quote: ... 5 g of silk cocoons were degummed in 2 L of boiling 0.02 M sodium carbonate (Catalog No. 451614, Sigma Aldrich, St. Louis, MO) in water for 30 minutes ...
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bioRxiv - Plant Biology 2022Quote: ... The cell walls of cleared samples were stained with 1 g L-1 calcofluor white (fluorescent brightener 28, Sigma, St. Louis, MO, USA) in ClearSee for 1 h and washed with ClearSee for 1 h ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then centrifuged and decanted again using the same conditions as the initial round and titrated to a pH of 4.50 using a 1 mol*L−1 solution of NaOH (Sigma Aldrich, St. Louis, MO) with a pH meter (Corning Pinnacle 530 ...
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bioRxiv - Microbiology 2020Quote: ... Meningococci were routinely grown on plates with GC agar Base (GCB) (Difco) containing 5 g/l agar (all chemicals were from Sigma unless stated otherwise) and Kellogg’s supplements (4 g/l glucose ...
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bioRxiv - Microbiology 2022Quote: ... 40 μL of 50 mM citrate buffer - pH 4.8 and 50 μL of 0.50% xylan solution (Megazyme, Ireland; Sigma-Aldrich, St. Louis, MO, USA). The reactions were incubated at 50 ° C for 10 minutes ...
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bioRxiv - Genomics 2022Quote: ... 1 ml of direct cardiac lineage differentiation medium (differentiation medium supplemented with 0.1 mg/ml L-Ascorbic acid (Sigma Aldrich, Cat. No. A7506) – DCLDM ...
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bioRxiv - Immunology 2019Quote: ... 8 fish per diet (4 fish per tank) were randomly selected and anaesthetized by immersion in 60 mg/l of tricaine methanesulfonate (MS-222, Sigma-Aldrich, MO, USA) and subsequently euthanized by a sharp blow to the head prior to dissection at the following sampling points ...
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bioRxiv - Neuroscience 2019Quote: ... the iDANs were split into single cells and 1-2x 104/cm2 cells were replated on a poly-L-ornithine (Sigma, 50 μg/ml) and murine Laminin (Sigma ...
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bioRxiv - Systems Biology 2019Quote: ... Inhibitors were added at the following concentrations: 40 μM 5,6-dichloro-l-β-D-ribofuranosyl benzimidazole (DRB) (D1916, Sigma-Aldrich, St. Louis, MO); 0.25 μM Flavopiridol (CS-0018 ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293 cells were seeded in 6-well plates (3.0 × 105 cells/well) coated with poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) and incubated for 24 h ...
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bioRxiv - Microbiology 2020Quote: ... HOCl treatment was done by mixing increasing molar ratios of HOCl (0, 5, 10, and 20 X) with concentrated RclA or L - Lactate Dehydrogenase (LDH, Sigma LLDH-RO 10127230001) (35 µM ...
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bioRxiv - Cell Biology 2020Quote: ... were cultured at 37°C with 5% CO2 in Minimum Essential Medium Eagle (with Earle’s salts, L-glutamine, and sodium bicarbonate) (Sigma-Aldrich, St. Louis, MO), supplemented with 10% heat inactivated Fetal Bovine Serum (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... nitrogen (N) and deuterium (D) isotopes were added: (i) light (L: R0K0) isotopes (12C14N on both Arg and Lys; Sigma-Aldrich: A6969, L8662), (ii ...
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bioRxiv - Microbiology 2021Quote: ... Water was collected within a depth of 0.5 m in 2 L plastic bottles and filtered within 4 h in the lab through 0.22 μm Sterivex® filters (EMD Millipore, Darmstadt, Germany) connected to a peristaltic pump (EMD Millipore ...
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bioRxiv - Evolutionary Biology 2021Quote: ... wAlbB Au (generation 5 post-microinjection) adults were fed 2 mg/mL tetracycline hydrochloride (≥95%, Sigma-Aldrich P/L, Castle Hill NSW, Australia) in a 10% sucrose solution for 10 days before blood feeding ...