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Citations for Millipore Sigma :
8001 - 8050 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... We spread the 50 μl of the sample with a delta cell spreader on square agar plates with 10% TSB supplemented with filter-sterilized cycloheximide and filter-sterilized MBOA (200 mg/L, Sigma-Aldrich, St. Louis, USA). For 10 days we incubated the plates at room temperature (21 - 25 °C) ...
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bioRxiv - Microbiology 2023Quote: Aliquots of 100 mg per human stool sample were suspended by gentle mixing in 1 ml of reduced PBS supplemented with 0.1% L-cysteine and 15% glycerol (Sigma‒Aldrich, St. Louis, MO, USA) in an anaerobic chamber (Whitley DG250 Anaerobic Workstation) ...
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bioRxiv - Physiology 2024Quote: ... Biotinylated proteins were eluted by incubation with 2x SDS-PAGE sample buffer containing β-mercaptoethanol (100 µmol/L, Cat. # M6250; Sigma-Aldrich, Darmstadt, Germany) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: The synthetic media used in this study contains 6.7 g/L yeast nitrogen base with ammonium sulfate (Conda Pronadisa #1545) and 2% glucose (SD) or 0.2% oleic acid (Sigma-Aldrich, St. Louis, MO, USA) + 0.1% Tween 80 (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: DNA indexes with different overhang sequences were validated by performing in-situ ligation assays on 4T1 mouse cells grown on 25mm round coverslips coated in poly-L-lysine overnight (Sigma P4707, 0.01% w/v). Cells were grown for 24-48hrs to ∼90% confluence.
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bioRxiv - Genomics 2024Quote: ... Pre-differentiated cells were subsequently counted and plated at 400,000-450,000 cells per well of a 12-well plate coated with Poly-L-Ornithine in 1 mL of Maturation medium with 20 uM trimethoprim (TMP) (Sigma-Aldrich, Cat No. 92131) to activate the CRISPRa machinery in these cells (TMP stabilizes the degron-tagged CRISPRa machinery) ...
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bioRxiv - Biochemistry 2024Quote: ... The loading reaction (480 µL) was spun in a pre-rinsed Amicon Ultra 0.5 mL Centrifugal Filter with 100 kDa MWCO (Merck Millipore, 6 000 g, 6 min). After two washes with water (480 µL ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant – less 300- 500 μL to avoid the pelleted material – was transferred using a multi-channel pipette into a 96- well filter plate (Millipore Sigma, cat. no. MVHVN4525). A 96-vacuum manifold (Millipore Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... and adapted to 2i condition by growing them in gelatin-coated flasks in N2B27 medium (50% neurobasal medium [Gibco], 50% DMEM/F12 [Gibco], 2 mM L-glutamine [Gibco], 0.1 mM β-mercaptoethanol, NDiff Neuro-2 supplement [Millipore], B27 serum-free supplement [Gibco]) supplemented with 1,000 units/mL LIF ...
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bioRxiv - Microbiology 2019Quote: ... Precipitated cells were resuspended in 200 µL of TE-buffer (10 mM Tris HCl, 1 mM EDTA, pH 8) containing lysozyme from chicken egg white (Sigma Aldrich, St. Louis, Missouri, USA) at a concentration of 15 mg mL-1 and incubated for 30 min at ambient temperature with regular mixing ...
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bioRxiv - Physiology 2020Quote: ... Silanized glass microelectrodes were then back-filled with 100 mmol L-1 KCl and front-filled with K+ ionophore (K+ ionophore I, cocktail B, Sigma Aldrich, St. Louis, MO, USA). A thinly pulled glass electrode (IB200F-4 ...
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bioRxiv - Microbiology 2019Quote: ... the temperature was reduced to 20 °C and gene expression induced by addition of 200 μg/l anhydrotetracycline (AHT, Sigma-Aldrich, St. Louis, MO, USA) for pASK-IBA vectors and/or 0.5 mM IPTG for pET and pCDFDuet plasmids ...
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bioRxiv - Molecular Biology 2019Quote: ... 36 were kept in neoteny and the other 36 were induced to metamorphosis using L-thyroxine (Sigma-Aldrich, St Louis, MO, USA, Cat. No. T2376) as described in (Demircan et al ...
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bioRxiv - Developmental Biology 2020Quote: ... Oocytes were collected by perforating the ampulla using a needle and cultured in a drop of KSOM + 1mM reduced L-glutathione (Millipore MR-020P-5F, Sigma G6013) under mineral oil (Sigma M8410 ...
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bioRxiv - Neuroscience 2020Quote: ... SND75 devices and Aclar sheets were coated separately using 100 μg/ml poly L-ornithine (PLO) (Sigma, St. Louis, MO USA, Cat N° P3655-100MG) in DPBS (Cat N° 14190250 ...
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bioRxiv - Neuroscience 2021Quote: ... differentiation was initiated by changing the medium to LaSR medium (Advanced DMEM/F-12 [Life Technologies], 2.5 mM GlutaMAX [Life Technologies], and 60 µg/ml L-ascorbic acid 2-phosphate magnesium [Sigma-Aldrich, St. Louis, MO]) supplemented with 7–8 µM CHIR 99021 (Tocris) ...
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bioRxiv - Plant Biology 2021Quote: ... (0.5x Murashige and Skoog salts [Caisson Labs], 1% sucrose, 0.5 g/L MES and 0.4% Phytagel [Merck Sigma-Aldrich], adjusted to pH 5.7 with KOH) or nitrogen limiting (-N ...
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bioRxiv - Plant Biology 2021Quote: ... (+N medium diluted 1/25 in 0.5X Murashige and Skoog medium without nitrogen [Caisson Labs], 0.5 g/L MES and 0.4% Phytagel [Merck Sigma-Aldrich], adjusted to pH 5.7 with KOH) growth medium (for detailed medium composition see Table S6) ...
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bioRxiv - Microbiology 2021Quote: ... the MDCK monolayers were washed twice to remove FCS using PBS containing 1 μg/mL of trypsin treated with N-tosyl-L-phenylalanyl chloromethyl ketone (TPCK) (Sigma-Aldrich, St. Louis, Mo, USA), inoculated with each sample ...
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bioRxiv - Plant Biology 2020Quote: ... Selected experiments were done with a high, but realistic (Blume et al., 2010) concentration of background electrolytes with following concentrations (Sigma Aldrich, DE, mmol L−1): NO3− 2.96 ...
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bioRxiv - Cell Biology 2021Quote: ... One of the samples was treated immediately for 25 minutes at 37°C with 300 nmol/L TMRM (Sigma-Aldrich Inc., St Louis, MO, USA) and was considered as our (control ...
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bioRxiv - Neuroscience 2022Quote: ... we used donkey anti-goat Igg H&L (Alexa Fluor® 647) Preadsorbed (Ab150135, 1:50) and sheep anti-rabbit Cy3 (Sigma–Aldrich C2306, 1:100).
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bioRxiv - Neuroscience 2022Quote: ... 4 well chamber slides (177437, Thermo Fischer Scientific) were coated by incubating for 12 h with filter sterilized poly-L-lysine (P8920, Sigma-Aldrich; 100 μ g/ml) in borate buffer (boic acid 3.1g/l ...
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bioRxiv - Neuroscience 2020Quote: ... were intracardially perfused with PBS (phosphate buffer solution with heparin 5000 IU/l, LEO-Pharma, Lysaker, Norway) and 4 % paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) in PBS at 4 °C for tissue fixation ...
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bioRxiv - Microbiology 2020Quote: ... The optimization of the analytical parameters in MS was performed by infusing the internal standard N-heptanoyl-L-homoserine lactone (C7-HSL) (Sigma-Aldrich, Saint Louis, MO, USA).
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bioRxiv - Microbiology 2022Quote: ... Studies evaluating flow of amino nitrogen from Stickland-fermented leucine to [U-13C]pyruvate generated in glycolysis used L-[15N]leucine (Sigma-Aldrich, St. Louis, MO, USA).
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bioRxiv - Neuroscience 2019Quote: ... that was fragmented and purified as described above was supplemented with 3 μl of formamide (biochemistry grade; Wako Pure Chemical Industries, Osaka, Japan) and overlaid with 80 μl of mineral oil (Sigma-Aldrich, St. Louis, MO, USA). DNA was completely denatured by incubating the tube at 99°C for 10 min ...
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bioRxiv - Biochemistry 2020Quote: ... serial 10 µm cryosections were collected starting at the superior edge of the optic nerve head on pre-labeled 1×3 mm glass slides coated with 10% poly-L-lysine (Sigma Aldrich, St. Louis, MO, USA) and maintained at 37° during sectioning ...
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bioRxiv - Neuroscience 2019Quote: ... transferred into 2-ml Eppendorf cups containing 500 μl of 1 M L-ascorbic acid (Sigma Aldrich, cat. no.: A7506, CAS no.: 50-81-7) and bead-based homogenized for 2 min using a Qiagen TissueLyser LT at a frequency of 50/s ...
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bioRxiv - Immunology 2021Quote: AM were thawed and rested at 37°C (5% CO2) for 18 hours in complete RPMI-1640 supplemented with 1% L-glutamine (Sigma-Aldrich, St Louis, Missouri, USA), 10% FBS and an antimycotic antibiotic containing 10,000U/ml penicillin ...
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bioRxiv - Immunology 2021Quote: ... Cytospins of 2×105 BALC were then created for both the treated and untreated control cells on poly-L-lysine-coated microscope slides (Sigma-Aldrich, Catalogue number: P0425-72EA), to be used for immunofluorescent staining.
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bioRxiv - Immunology 2021Quote: Cryopreserved cells were thawed in a waterbath (37°C) and transferred to 10ml of warmed complete RPMI-1640 media (supplemented with 1% L-glutamine (Sigma-Aldrich, St Louis, Missouri, USA) and 10% FBS) ...
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bioRxiv - Microbiology 2022Quote: The AHL signaling molecules used in this study are N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL, Sigma-Aldrich CAS# 168982-69-2), N-butyryl-homoserine lactone (C4-HSL ...
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bioRxiv - Bioengineering 2022Quote: ... Casein (C5890) in powder, Phosphate-buffered saline (PBS, P4417) in tablets and Poly-L-Lysine (PLL, P2636) were purchased from Sigma-Aldrich (Burlington, MA, United States). Anti-CD9 antibody conjugated with VioBlue (#130-118-809 ...
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bioRxiv - Plant Biology 2022Quote: ... Pellets were resuspended in 620µl of nuclei lysis buffer (50mM Tris-HCl, 10mM EDTA, 1% SDS, 2mM PMSF, 2µg/ml protease inhibitor (Sigma-Aldrich, catalog No. 200-664-3). The suspensions were sonicated for 20min with the Bioruptor® Sonication System (Diagenode ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were infected 12.5 µL of concentrated lentivirus pTRE-SadCas9-2xKRAB-dtTomato and 12.5 µL of concentrated lentivirus pCMV-rtTA in 400 µL of StemFlex medium supplemented with 6 ug/mL of polybrene (Millipore, Cat. No. TR-1003-G). After 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... the electrode area was covered with 20 μL of 0.05 mg/mL poly-L-ornithine (PLO) solution (cat. A-004-C, Sigma-Aldrich, Saint Louis, MO, United States) and incubated at 37°C for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: ... All injections were administered at a 0.1 μL/min infusion rate using a 28-gauge needle attached to a 2 μL or 5 μL Hamilton microsyringe (Sigma-Aldrich, St. Louis, MO, USA). The injection cannula was left in place for 15 min after the infusions before retracting slowly ...
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bioRxiv - Neuroscience 2022Quote: ... the culture medium was completely replaced with fresh basic media and a 48-hour puromycin selection (1 mg/l, catalog#P8833-10MG, Sigma-Aldrich, St. Louis, MO, USA) period was started ...
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bioRxiv - Genetics 2024Quote: ... We found that only using hydrophilic Nunclon® Δ surface treated flask allowed for appropriate cell adherence (Sigma, Cat. No. F7552-1CS. We also modified the additional coating of poly-L-ornithine (Sigma, Cat. No. P-3655-10MG) and fibronectin (MilliporeSigma ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension was plated at a density of 5 × 105 cells/mL on cell culture plates pre-coated with poly-L-Ornithine (Sigma-Aldrich, St. Louis, MO, USA) in Neurobasal medium [Neurobasal-A medium (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... with the exception of Akkermansia muciniphila DSMZ 22959 that was pre-cultured in M2-based medium supplemented with 3 g/L of type II mucin (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland), and Veillonella parvula DSMZ 2008 that was pre-cultured in M2-based medium supplemented with 60 mM of DL-lactic acid 90% (Sigma-Aldrich Chemie GmbH ...
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bioRxiv - Molecular Biology 2023Quote: ... 300 ng of F(ab’)2-Rabbit anti-Rabbit IgG (H+L) (CST, 4412S, Lane Danvers, MA, USA) was diluted in 0.1% Triton X-100 (Sigma-Aldrich, T8787, St. Louis, MO, USA) and incubated at 95°C for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... Both cell lines were monthly tested for mycoplasm contamination and maintained in culture in RPMI 1640 medium with L-glutamine and 125 μM HEPES (Sigma Aldrich, St. Louis, MO, USA) and 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Tomato plants were placed into 121 L transparent methacrylate chambers containing hydrophilic cotton swabs soaked with 5 μM of (Z)-3-hexenyl butyrate (HB; Sigma-Aldrich, Saint Louis, MO, USA) or distilled water in the case of control plants ...
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bioRxiv - Neuroscience 2023Quote: ... the DRGs have been stripped of their nerve roots which branch off from the body and placed on glass coverslips previously coated with 500 μg ml-1 poly-L-lysine (PLL, Sigma-Aldrich, Burlington, Massachusetts, US, #P4707) and 10 μg ml-1 laminin (Gibco ...
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bioRxiv - Genomics 2023Quote: ... neoformans parental strains or segregants was set up by picking a fungal colony from YPD agar plates (50g/L YPD broth powder [Sigma-Aldrich], 2% Agar [MP Biomedical]) and resuspending it in 3mL liquid YPD broth (50 g/L YPD broth powder [Sigma-Aldrich]) ...
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bioRxiv - Biochemistry 2023Quote: 50 mkL of cell suspension from section 2.1 was pelleted and suspended in 50 mkL of 10 mM or 50 mM L-NAME solution in water (Sigma-Aldrich, St. Louis, MO, USA). The samples were incubated in a ThermoMixer for 1 h at 30° ...
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bioRxiv - Cancer Biology 2023Quote: ... and BIT (bovine serum albumin 4 g/L, insulin 5 µg/mL, and transferrin 60 µg/mL, all from Sigma-Aldrich, Saint Louis, MO, USA). To facilitate optimal cell growth and maintenance ...
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bioRxiv - Bioengineering 2023Quote: ... and (GT)15 single-stranded DNA (10 g/L, Integrated DNA Technologies, Coralvile, IA, USA) suspended in 1X PBS (phosphate-buffered saline, Sigma-Aldrich, St. Louis, MO, USA) were added to a final oligonucleotide:SWCNT mass ratio of 2:1 ...