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Citations for Millipore Sigma :
7751 - 7800 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and immediately digested in Cystein-activated Papain solution (17U/ml Papain from Worthington, 20µg/ml DNase I from Sigma, 2mg/ml of L-Cysteine Hydrochloride from Sigma in Dissociation media) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The brain was excised and rapidly immersed in oxygenated ice-cold aCSF containing (in mmol/L): 118 NaCl (#S9888; Sigma, Poole, UK), 3 KCL (#P3911 ...
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bioRxiv - Microbiology 2021Quote: ... Each water sample collected (~ 10 L) was immediately filtered through a 0.22 μm polycarbonate membrane (diameter 45 mm; Millipore, Bedford, MA, USA) using a peristaltic pump ...
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bioRxiv - Microbiology 2020Quote: ... and susceptible (all MIC≤ 1mg/L) strains (S1 to S4) were incubated in cation adjusted Mueller Hinton II Broth II (MHB2, Sigma-Aldrich, USA) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and adhered to 12 mm diameter round glass coverslips (Paul Marienfeld GmbH & Co. KG, Germany) previously coated with 0.01% poly-L-lysine (Sigma-Aldrich, Darmstadt, Germany) for 20 minutes ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Molecular Biology 2021Quote: Leishmania donovani Bob strain (LdBob strain/MHOM/SD/62/1SCL2D) (WT-L. donovani) promastigotes were cultured at 26°C in M199 medium (Sigma-Aldrich, USA), supplemented with 100 units/ml penicillin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... The GIFT were maintained in well-aerated water and treated with 200 mg/L tricaine methane sulfonate (Sigma, St Louis, MO, USA) for rapid deep anaesthesia ...
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bioRxiv - Immunology 2020Quote: Tyrosine hydroxylase activity was inhibited by treating B cells with different concentrations of 3-Iodo-L-tyrosine (Sigma-Aldrich, catalogue number: 18250), 30 min before activation with anti-IgM/CpG.
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bioRxiv - Immunology 2020Quote: ... Frozen tissue sections (4 μM) were cut at −27°C and mounted on poly-L-lysine (Sigma Chemical Co., Saint Louis, USA) coated slides ...
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bioRxiv - Cell Biology 2020Quote: YFP+ cells were plated at a minimum seeding density of 1 × 105 cells/well on fibronectin-coated (2% w/v in 0.1mol l−1 PBS, Sigma-Aldrich, Gillingham, UK) 6-well dishes ...
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bioRxiv - Microbiology 2021Quote: ... Positive protein-protein interactions were subjected to another round of stringent selection by testing the yeast transformant’s ability to express α-galactosidase from the MEL1 reporter gene (blue/white screening) when selected on SD/-T/-L/-H agar plates supplemented with X-α-Gal (Sigma, USA) at 30°C.
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bioRxiv - Neuroscience 2021Quote: ... and perfused with 40 ml of 0.1 M/L phosphate-buffered saline and 40 ml of 4% (w/v) PFA (Sigma, 30525-89-4) 24h after social interaction ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were spin-infected at a 0.3 multiplicity of infection in the presence of DMEM (10% FBS, 1% L-Glutamine) and hexadimethrine bromide (8ug/ml) (Sigma-Aldrich, 107689) at 2000 rpm ...
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bioRxiv - Biophysics 2021Quote: Mica was freshly cleaved and then pre-treated by depositing 20 μl 0.01% (w/v) 1-5 kDa poly-L-lysine (Sigma-Aldrich, MO, USA), left for 5 mins before washing with 1000 μl filtered milliQ H2O and finally vacuum drying ...
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bioRxiv - Neuroscience 2022Quote: ... The 24- or 96-well plates used for neuronal differentiation were coated with poly-L-ornithine (Sigma-Aldrich, P3655; 20 μg/mL) overnight at room temperature (∼26°C) ...
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bioRxiv - Cancer Biology 2022Quote: ... FFPE samples were sectioned at 5 μm and mounted onto 22 mm x 22 mm coverslips coated with poly-L-lysine (Sigma-Aldrich, P8920) coated according to Akoya’s CODEX user manual ...
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bioRxiv - Bioengineering 2022Quote: ... the content of the well was transferred into an Eppendorf tube and incubated with 500 μL digestion buffer (500 mM phosphate buffer, 5 mM L-cystein (Sigma-Aldrich, C1276), 5 mM EDTA (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... 0.5 mL of papain digestion buffer (100 mm sodium phosphate buffer containing 10 mm L-cysteine [Sigma-Aldrich], 125 μg mL−1 papain [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids were transformed into Escherichia coli BL21 (DE3) and were plated onto a petri dish containing lysogeny broth (LB) media treated with 100 mg/L kanamycin (Sigma-Aldrich, USA). The seed culture was prepared by picking a single colony from the petri dish ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections of the paraffin-embedded embryos (3-5 µm) were attached to poly-L-lysine-coated glass slides (Sigma, St. Louis, Mo) dried at 55 °C for 1 h and de-waxed twice in xylene (10 min each) ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were generated by culturing marrow cells in poly-L-lysine coated culture plate for 7 days with 50 ng/ml M-CSF in RPMI 1640 (Sigma-Aldrich, R8758) supplemented with 20% FBS ...
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bioRxiv - Plant Biology 2022Quote: ... The seeds then were placed on 0.75% phytoagar Petri dishes supplemented with half-strength (0.5 g/L, pH 5.8) Murashige-Skoog salt (Sigma-Aldrich, USA; Cat. No. M5524). The dishes were placed in a growth chamber (Panasonic ...
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bioRxiv - Microbiology 2022Quote: ... the white embryo was placed in Murashige and Skoog (MS) agar medium (2. 25 g/L MS (Sigma-Aldrich; Castle Hill, Australia), 10 g/L agar ...
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bioRxiv - Systems Biology 2022Quote: ... Yeast were transformed as described previously52 with the pHLUM plasmid53 and selected for growth on SD media with inositol (5 g/L ammonium sulfate (Sigma-Aldrich: A4418), 1.7 g/L Yeast Nitrogen base (BD Biosciences ...
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bioRxiv - Systems Biology 2022Quote: The indicated yeast mutants were cultivated in a 96-well format in SD media (5 g/L ammonium sulfate (Sigma-Aldrich: A4418), 1.7 g/L Yeast Nitrogen base (BD Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... 500 to 1000 μl L-15 full medium were added to the suspension and the solution was seeded on 1 - 2 peanut lectin (Sigma-Aldrich, USA) coated glass bottom petri dishes (ibidi ...
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bioRxiv - Immunology 2022Quote: ... A total of 1×106 isolated neutrophils were attached to coverslips coated with poly-L-lysine solution 0.1% (Sigma-Aldrich; cat. P8920) incubated for 4 h at 37°C for NET immunostaining ...
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bioRxiv - Molecular Biology 2022Quote: ... pombe cells expressing Luc7-HTP was grown independently in 800 mL of 1X EMM media (EMM Broth, Formedium, Cat #PMD0210) supplemented with 2% glucose and 125 mg/L each of adenine (Sigma, Cat #A2786), L-histidine (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... CD14+ monocytes were seeded at 106 cells/ml and cultured in R10 media (RPMI supplemented with FBS, HEPES, L-glutamine, and pen/strep) with 50 ng/ml of GM-CSF (Sigma Aldrich G5035) in a 37°C incubator ...
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bioRxiv - Physiology 2022Quote: ... 10 μL of the lower hydrophobic phase was extracted with gel loading pipet tips and spotted onto a silica plate (EMD Millipore #M116487001) for thin layer chromatography ...
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bioRxiv - Physiology 2022Quote: ... 20 μL of the lower hydrophobic phase was extracted with gel loading pipet tips and spotted onto a silica plate (EMD Millipore, M116487001) for thin layer chromatography ...
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bioRxiv - Biophysics 2024Quote: ... The stock solution was diluted 1:100 with distilled water and placed on a glass-bottom 35mm dish coated by Poly-L-lysine solution (Sigma Aldrich, P8920).
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bioRxiv - Biochemistry 2024Quote: ... 200 μl anti-FLAG M2-HRP was added and incubated for 90 min (prepared in 1% BSA, 1:10,000) (Sigma, Cat. no. A8592). Post blocking ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... the animals were kept in the dark for at least three days in fresh vials in which the food was soaked with 50 µL of a 100 mmol L−1 all-trans-Retinal solution (R2500; Sigma-Aldrich, RRID:SCR_008988).
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bioRxiv - Neuroscience 2024Quote: ... In-vivo imaging was performed on larvae embedded in 1.5 % low melting point agarose in E3 medium supplemented with 4.2 g/l MS-222 (Sigma-Aldrich, St. Louis, USA). Confocal imaging was done with a Zeiss LSM 780 microscope using Plan-Apochromat 10x/0.3 Air or Plan-Apochromat 20x/0.8 Air objectives ...
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bioRxiv - Neuroscience 2023Quote: ... horizontal shaker) in digestion medium (RPMI-1640 supplemented with L-glutamine, Pen/Strep, β-ME, HEPES, Sodium Pyruvate, Liberase TLTM (Sigma Aldritch) and DNase I) ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 adherent cell lines with selected genetic background were seeded on 10-cm dishes previously coated with poly-L-lysine (Sigma, 0.1mg/mL). 10µg of plasmid were transfected on each 10-cm dish using 30µg polyethyleneimine (PEI ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were adhered onto 5 mm glass coverslips coated with poly-L-lysine and mounted onto the LLSM sample holder using Dow Corning high-vacuum silicone grease (Sigma-Aldrich, #Z273554). The imaging chamber was maintained at a temperature of 34°C and continuously perfused with aCSF (in mM ...
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bioRxiv - Molecular Biology 2024Quote: ... Exponentially proliferating cells were grown to confluence and then left at confluence for 48 hours before incubation in differentiation media (DMEM supplemented with 10% FBS, 4 mM L-Glutamine, 100 U/ml penicillin/streptomycin, 1 μM dexamethasone (Sigma, D-2915), 1 μg/ml insulin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Adjustment of lactic acid concentration in cell-free supernatants was performed by dilution of a 2M L-lactate stock (Sigma-Aldrich, Belgium) to 140 mM in GASSN.
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bioRxiv - Microbiology 2024Quote: ... were grown in BHI-S liquid media composed of Brain Heart infusion (BHI) broth (Oxoid, #CM1135B) (37g/l) supplemented with 10% FBS (Sigma Aldrich, #F7524) and vitamin K3 at 1 μg/ml ...
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bioRxiv - Microbiology 2024Quote: ... was grown (~25°C) in TB medium with 2% DMSO with or without 150 mg/L acyclic naphthenic acids (Sigma Aldrich 70340,15), or 150 mg/L of custom mix of 9X naphthenic acids (Table S1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Then cells were washed with PBS and treated for 20 hours with a medium containing a combination of glucagon and corticosterone together with L-Glutamine (20 mM, Sigma, cat# 59202C): After 20 hours ...
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bioRxiv - Immunology 2023Quote: ... Surface display of Omicron RBD was induced by passageing the cells into SG-CAA medium [20 g/L galactose (Sigma-Aldrich, G0625), 8.56 g/L NaH2PO4·H2O ...
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bioRxiv - Immunology 2023Quote: ... The cells were recovered for 1 h in in recovery medium (YPD:1 M Sorbitol solution mixed in a 1:1 ratio) before passageing the cells into selective SD-CAA medium [20 g/L glucose (Sigma-Aldrich, G8270), 8.56 g/L NaH2PO4·H2O (Roth ...
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bioRxiv - Molecular Biology 2022Quote: ... and HEK293T LLP-Int-Blast (the parental cell line acting as negative control, NEG) were seeded in poly-L-ornithine (Sigma, Cat#P3655) coated 96-well plates at 40,000 cells/well (100 uL volume ...
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bioRxiv - Microbiology 2023Quote: ... 2021) in trypticase soy agar (TSA) (Oxoid®, United States) enriched to 25 mg/L BAC (≥95.0% Fluka 12060 Sigma, United States). The 16S rRNA gene was sequenced to identify the bacterial species ...
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bioRxiv - Microbiology 2022Quote: All PCR products were analysed by electrophoresis in a 1.5% agarose gel stained with 1µ L fluorescent stain for dsDNA (Nancy 520®, Sigma-Aldrich, Brazil), visualized under UV light ...
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bioRxiv - Microbiology 2023Quote: ... Mf extracted from the jird peritoneal cavity were filtered and cultured in RPMI-1640 with L-glutamine (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...