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Citations for Millipore Sigma :
7451 - 7500 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... were passaged 2-3 times weekly and grown at 37° C with 5% CO2 in DMEM containing 10 mM L-glutamine (Sigma, M8537), supplemented with 10% heat-inactivated FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... Macrophage-like THP1 cells were pre-incubated with L-glutamic acid (5mM) or Sodium Pyruvate solution (2mM, Sigma catalog# S8636-100ml) for 2 hours before stimulating with Escherichia coli serotype O127:B8 LPS (50ng/ml ...
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bioRxiv - Synthetic Biology 2020Quote: ... and started with a batch phase using 400 mL synthetic medium containing 20 g/L of glucose and 5 mL Antifoam 204 (Sigma A6426). The temperature was maintained at 30°C and the pH was kept constant at 5.0 by dropwise addition of a 10M KOH solution ...
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bioRxiv - Bioengineering 2021Quote: ... 1 mg L-1 of the suspended SWNCTs were added to the wells of a 96 well plate to which insulin (Sigma-Aldrich) was added at a final concentration of 33 µg ml-1 ...
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bioRxiv - Neuroscience 2019Quote: ... samples were incubated at 37°C overnight and inhibition of γ-secretase mediated ICD formation was performed using 1 µM L-685,458 (Sigma, L1790). In the last step ...
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bioRxiv - Immunology 2020Quote: ... 1×105 neutrophils were seeded into a white 96-well plate (Costar 3917) in the presence of the luminol reagent (1 mmol/L; Sigma-Aldrich). Conidia (MOI 1:2 ...
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bioRxiv - Immunology 2020Quote: ... TLR-2KO and TLR-4KO (1.5×105) mice were seeded onto round coverslips previously treated with poly-L-lysine (Sigma-Aldrich®) and placed at the bottom of 24 well plates ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Recovering Drosophila were fed with standard lab fly food (control) or standard lab fly food mixed with 5 mM L-Leucine (Sigma L8000), 5 mM L-Glutamine (Sigma G3126) ...
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bioRxiv - Immunology 2021Quote: ... with sections being cut 7µM thick and mounted onto poly-L-lysine-coated microscope slides (Sigma-Aldrich, Catalogue number: P0425-72EA). The cryosections were numbered in order of cutting to allow for the “position” within the structure to be inferred and picked for specific staining ...
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bioRxiv - Immunology 2020Quote: ... Cryptococcus was grown for 18 hours overnight in 2 ml yeast peptone dextrose (YPD 50 g/L Fisher, 2% agar Sigma-Aldrich) medium ...
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bioRxiv - Biochemistry 2020Quote: ... the lysate (1 mg of protein in 500 μl) was aliquoted per sample and then the following were added: 10 μl of 5 mM biotin picolylazide (900912 Sigma-Aldrich) and 50 μl of click reaction mix (three parts TBTA 5 mM TBTA in butanol:DMSO (4:1 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... inserted into a falcon tube (50 mL) and fed with either 10 mM L-phenyl-D5-alanine (Sigma-Aldrich, Cat# 615870) or water for 24 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were maintained in Roswell Park Memorial Institute 1640 (RPMI-1640) medium with L-glutamine and sodium bicarbonate (R8758, Sigma-Aldrich), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2019Quote: ... NSCs used in positive control experiments were seeded on conventional polystyrene culture plates coated with poly-L-ornithine and fibronectin from bovine plasma (P+F) (Sigma-Aldrich) to which uncoated polystyrene dishes served as negative controls ...
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bioRxiv - Plant Biology 2020Quote: ... For pipecolic acid treatment five-week-old plants were either watered with 10 ml water or 1 mM D/L-pipecolic acid (Pip; Sigma-Aldrich) solution as described before (Návarová et al. ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of cell supernatant was transferred to a 96-well plate and incubated with an equal volume of sulphanilamide (Sigma, S9251) and N-alpha-naphthyl-ethylenediamine (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... washed K-562 cells were resuspended in a small volume of PBS at a density of 1 million cells per ml and cell suspension was applied to poly-L-lysine (Sigma P1399) coated glass coverslips (1.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary hippocampal neurons were plated at density of 120 cells/mm2 on 3.5-cm diameter Petri dishes (Falcon® 35 mm, 353001) treated for 24 h with poly-L-lysine (0.1 mg/ml; Sigma-Aldrich) in borate buffer (0.1 M) ...
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bioRxiv - Microbiology 2019Quote: ... 25 μl of the supernatant were hydrolyzed and processed as described in the MAK016 assay kit instructions for colorimetric assays (Sigma-Aldrich). For the replenishment experiment ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... half of the EtOH-exposed dams were exposed to either THC (100 mg/mL at 2 L/min airflow) or the Vehicle (propylene glycol; Sigma-Aldrich) for 30 min via an e-cigarette tank ...
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bioRxiv - Plant Biology 2021Quote: ... Sterilized seeds were germinated on Murashige & Skoog (MS) medium [1xMS: 4.4 g/L Murashige and Skoog medium (Sigma Aldrich; Missouri, USA), pH 5.7 w/KOH ...
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 2-5 × 104 cells /well on coverslips in a 24-well plate pre-coated with poly-L-lysine (Sigma, P9155) and cultured in a Neurobasal medium supplemented with 2% B27 supplement ...
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bioRxiv - Synthetic Biology 2021Quote: ... all chemicals were used as by Sun et al.33 except for amino acids (L-amino acids set, Sigma #LAA21-1KT).
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were maintained in high amino acid medium (DMEM high glucose, 4 mM L-Glutamine, no sodium pyruvate (Sigma Aldrich), 1x MEM non-essential amino acids (Sigma Aldrich) ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: ... All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich). Hemin (H9039 ...
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bioRxiv - Cell Biology 2019Quote: ... The solutions were vortexed and the click reactions initiated by addition of (+)-sodium L-ascorbate (4.2 mM, Sigma-Aldrich, Cat#: A7631). The reactions incubated for 6 hours at room temperature and subsequently purified by reverse phase HPLC as described above ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 520-550nm emission) and 470 nm for chlorophyll autofluorescence (filter set 50, 665-715nm emission) with cells in poly-L-lysine (Sigma Aldrich) coated 8-well μ-slides (Ibidi) ...
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bioRxiv - Microbiology 2020Quote: ... was maintained in MEM (Minimal Essential Medium) containing 10% (v/v) foetal bovine serum,10,000 U penicillin/streptomycin and 2% (v/v) L-glutamine (Sigma Aldrich, Germany). Both SARS-CoV-2/FFM1 and SARS-CoV-2/FFM7 were isolated as previously described (Hoehl et al. ...
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bioRxiv - Microbiology 2020Quote: ... 100 μl was pipetted onto Sabouraud dextrose agar (SDA) in 100-mm Petri dishes supplemented with 50 μg/ml chloramphenicol (Sigma Aldrich) and 5 μg/ml gentamicin (Research Products International) ...
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bioRxiv - Immunology 2021Quote: ... aeruginosa strains with the arabinose-inducible vector and its derivatives were grown in LB with 133 mM L-arabinose (2% w/v) and 300 μg/ml carbenicillin (both from Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... 6000 OVCAR3 cells were seeded on the wells of a flat-bottom 96-well plate in RPMI containing L-glutamine (R8758, Sigma-Aldrich), supplemented with penicillin/streptomycin (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... frozen patient tumor fragments were slowly thawed at 37°C and extensively washed in PBS and warm RPMI medium containing L-glutamine (R8758, Sigma-Aldrich), supplemented with penicillin/streptomycin (100 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... The evaluation of the effect of reactive oxygen species was done by treating the cells for 6h with 10 mM of N-Acetyl-L-Cysteine (Millipore, 1009005).
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bioRxiv - Bioengineering 2021Quote: Day-21 TSC2+/+ organoids were incubated in Advanced RPMI + 1X L-GlutaMAX with 50ng/ml human IL1-β (Sigma-Aldrich # H6291) for 96hs ...
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bioRxiv - Cell Biology 2021Quote: Human foreskin fibroblasts HFF-1 (ATCC) were cultured with DMEM (High Glucose) including l-glutamine and phenol red (Wako) supplemented with 15% FBS (Sigma-Aldrich) and 1% penicillin-streptomycin solution (Wako ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 0.5 % v/v StemSure 10 mmol/l 2-mercaptoethanol solution (Wako) and 10% v/v fetal bovine serum (#F7524; Sigma-Aldrich), as the cell medium ...
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bioRxiv - Microbiology 2020Quote: ... All Strain were maintained in 40 % glycerol, 60 % Lysogeny Broth (LB, 1 /L, 15 g Agar, 10 g Tryptone (Sigma-Aldrich), 5 g Yeast Extract(Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... we treated 1 or 14 days primary cultures of hippocampal neurons O/N with 15 mM L-glutamic acid (LGA; Sigma-Aldrich). Total RNA concentration and purity (260/280 and 260/230 ratios ...
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bioRxiv - Cancer Biology 2022Quote: ... GSCs were made adherent by withdrawing EGF/FGF from the growth medium and culturing on glass slips coated with poly-L-ornithine (1:100; Sigma, P4957), fibronectin (1:60 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in high glucose Dulbecco’s Modified Eagle’s Medium (DMEM) containing 4.5 g/l D-glucose (Sigma Aldrich, St. Louis, MO) supplemented with 10% fetal calf serum (FCS) ...
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bioRxiv - Molecular Biology 2022Quote: Brugia malayi and Brugia pahangi adults extracted from the Meriones unguiculatus infection system (NIH/NIAID Filariasis Research Reagent Resource Center) [102] were maintained in daily changes of RPMI 1640 with L-glutamine (Sigma-Aldrich) supplemented with FBS (10% v/v ...
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bioRxiv - Cell Biology 2022Quote: ... medium was exchanged with 900 μl of fresh growth medium before cells were transfected with 40 nM of esiRNA against human FXR (MISSION esiRNA, Sigma, EHU133161). EsiRNA was diluted in Opti-MEM I Reduced Serum Medium (Thermo Fisher) ...
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bioRxiv - Bioengineering 2022Quote: ... were weighted and digested overnight in papain digestion buffer (containing 100 mmol phosphate buffer, 5 mmol L-cystein, 5 mmol EDTA and 140 μg/ml papain (P4762, Sigma-Aldrich)) at 60 °C ...
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bioRxiv - Bioengineering 2022Quote: ... 0.2 μL of reverse primer (both at 10 μmol L- 1) and 7.6 μL of molecular grade water (Sigma Aldrich, USA). Three qPCR technical replicates were prepared for each sample ...
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bioRxiv - Developmental Biology 2022Quote: ... fish were incubated in freshly prepared tank water containing 30 mg/l of the halogenated thymidine analog 5-bromo-2’-deoxyuridine (BrdU; Sigma-Aldrich) for periods between 12 h and 24 h at various time points following experimental intervention and kept in the dark at 28°C ...
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bioRxiv - Cell Biology 2022Quote: ... The medium was changed to phenol-free Seahorse XF DMEM (10 mM glucose, 1mM pyruvate and 2 mM L-glutamine (all from Sigma Aldrich). The OCR was measured in a Seahorse XFe96 Flux Analyser with Seahorse Wave 2.4 software (Agilent) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were cultured in 4 L of Luria Bertani broth (LB) medium with 50 mg/mL carbenicillin (Sigma-Aldrich; C1389) and induced with 0.5 mM isopropyl β-d-thiogalactopyranoside (IPTG) ...
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bioRxiv - Genomics 2022Quote: ... culture medium was replaced with S1-media (Final 11,6 g/L MCDB131, Sigma Aldrich, M8537-1L; 2 mM D-+-Glucose, Sigma Aldrich, G7528-250G ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the yeast library was inoculated into liquid SDCAA (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... The fused embryos were treated with 5 μmol/L ionomycin for 4 min and then incubated in mSOF medium (Sigma, USA) containing 2 mmol/L 6-Dimethylaminopurine(6-DMAP ...