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Citations for Millipore Sigma :
7501 - 7550 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... difficile spores were maintained on brain heart infusion (BHI) medium supplemented with 100 mg/liter L-cysteine and 0.1% taurocholate (Sigma-Aldrich, T4009). Cultures were started by inoculating a single colony from the plate into BHI liquid medium supplemented with 100 mg/liter L-cysteine and grown anaerobically ...
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bioRxiv - Microbiology 2022Quote: ... The culture plates were prepared by coating with 0.001% poly-L-lysine solution (Millipore Sigma, P4707, diluted in water 1:10) for plastic surfaces and 100 µg/ml poly-L-lysine hydrobromide (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... lidocaine (2 μg/L) was used as an analgesic and added to the embryo medium (together with 200 μg/ml tricaine (Sigma-Aldrich)) prior to injection ...
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bioRxiv - Microbiology 2022Quote: ... 100 μL of overnight cultures of Pseudomonas putida KT2440 and Staphylococcus epidermidis ATCC 12228 grown in LB and BHI broth (Sigma # 53286) respectively were washed twice in PBS + 1% Pluronic F68 then resuspended in 100 μL of PBS + 1% Pluronic F68 ...
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bioRxiv - Microbiology 2022Quote: ... A 500 μL aliquot was collected every 10−50 s (depending on the virus) and mixed with 5 μL of 5,000 mg L−1 sodium thiosulfate (Sigma-Aldrich, Germany) to instantly quench the residual free chlorine ...
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bioRxiv - Plant Biology 2022Quote: ... They were cultivated for 3 weeks on MS-derived shoot induction medium containing 0.5 mg L-1 IAA and 2.5 mg L-1 BAP and supplemented with sodium salicylate (0.0, 0.1, 1.0, and 10.0 μM; Sigma–Aldrich, St. Louis, MO, USA). Callus frequency (CF ...
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bioRxiv - Neuroscience 2022Quote: ... Neurons were purified on a 10% bovin serum albumin (sigma Aldrich) cushion and plated on Poly-L-lysine (10mg/mL Sigma Aldrich) and Laminin (0,5mg/mL-Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated either on 13 mm glass coverslips (40,000 cells) or three-compartments MFCs (100,000 cells; Figure 1A) coated with 1 mg/ml poly-L-lysine (Sigma-Aldrich, #P2636) in borate buffer (0.15 M ...
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bioRxiv - Neuroscience 2022Quote: ... that was precoated with poly-L-ornithine for 1 hour at 37°C (PLO; 10 μg/mL plastic, 50 μg/mL glass, Sigma P3655) and laminin for 3 hours at 37°C (5 μg/mL ...
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bioRxiv - Plant Biology 2022Quote: ... 20 μL of apoplastic fluid was combined with 30 μL of substrate solution (4-methylumbelliferyl-β-D-N,N′,N″-triacetylchitotrioside, Sigma) to a final substrate concentration of 18 μM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were initially diluted to 0.5×106 cells/mL in 1.2 L EX-CELL Advanced CHO Fed-batch Medium (Sigma, Cat. No. 14366C). Starting from day 3 ...
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bioRxiv - Plant Biology 2023Quote: ... 5 μL lysate was mixed with 45 μL MUG (methylumbelliferyl b-D-glucuronide) solution (10 mM Tris/HCl pH 8, 2 mM MgCl2, 1 mM MUG (Sigma Aldrich)) in a 96-well plate ...
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bioRxiv - Immunology 2022Quote: ... neutrophils were plated in 24-well plates containing glass coverslips covered with 0.01% poly-L-lysine solution (Sigma-Aldrich; cat. P8920), fixed with PFA 4% at RT for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... then 50 μl of each suspension were administered per fish by intramuscular injection at a point midway between the insertion of the dorsal fin and the lateral line following anesthesia (50 mg/L, MS222, Sigma-Aldrich). For F ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Selection experiments in the presence of antibiotic(s) were performed in Müller-Hinton broth (MH broth, 23 g/l, Sigma-Aldrich, UK). MH is a preferred medium for use with antibiotics 99 ...
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bioRxiv - Neuroscience 2022Quote: ... we provided a buprenorphine solution (0.1 g/L, Temgesic®) mixed in drinking water containing 5% (w/v) D-Glucose (G8769-100ml, Sigma Aldrich) to mask the drug taste ...
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bioRxiv - Bioengineering 2022Quote: ... dry weights of scaffolds were collected and samples were digested overnight in papain digestion buffer (containing 100 mmol phosphate buffer, 5 mmol L-cystein, 5 mmol EDTA and 140 µg/ml papain (P4762, Sigma-Aldrich)) at 60 °C ...
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bioRxiv - Bioengineering 2022Quote: ... cell-laden microgels were demulsified and resuspended by an excessive amount of LB media spiked with 10−6 mol/L 3-Oxo-C12-HSL (Sigma-Aldrich) before incubation at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... DMEM/F12 + HEPES (15mM) + Sodium Bicarbonate (3.6mM) + L-glutamine (4mM)+ Insulin (10ug/mL) +Tranferrin (5ug/mL) (or ITS,1x, Sigma Aldrich #I3146) + Cholera Toxin (0.1ug/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Semi-thin sections on poly-L-lysine coated glass slides were circled with a liquid blocker Dako-Pen (Sigma Aldrich, USA). The samples were washed and pre-incubated for 30 minutes at RT with 2% BSA in PBS to block non-specific binding sites ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Plant Biology 2024Quote: ... 6 ml of the yeast culture was inoculated in 300 ml of YPGE containing adenine {10 g/L yeast extract (Sigma 92144), 10 g/L peptone (Melford P20P240) ...
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bioRxiv - Neuroscience 2023Quote: ... 12 mm (Marienfeld Superior; Cat Number: 633029) coated with 1 mg/mL poly-L-lysine (Sigma Chemical Co; Cat Number: P2636) at a density of 2000 cells/cm2 in minimum essential medium (MEM ...
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bioRxiv - Microbiology 2024Quote: ... aeruginosa strains with the arabinose-inducible vector and its derivatives were grown in LB with 133 mM L-arabinose (2% w/v) and 300 μg/ml carbenicillin (both from Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... were affixed to the glass and 50 µL of poly-L-lysine (70k-150k molecular weight, Sigma-Aldrich, 25988-63-0) were added to the inside of the chamber on the glass coverslip and incubated for at least 30 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... grown antral follicles were free from alginate encapsulation by digestion in an L-15-based lysis solution containing 1% FBS and 10 IU/mL alginate lyase (Sigma-Aldrich) for 20 minutes at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... transformed with pET-28a-6His-SUMO-HeVN was inoculated in a 1/500 ratio into 2xYT medium with kanamycin (16 gr/l Tryptone Plus (Sigma Aldrich), 10 gr/l Yeast Extract (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... were grown in complete growth medium (CGM) that consisted of 12g/L of Dulbecco’s minimum essential medium (DMEM)/F12 (Sigma, USA D0547) supplemented with 5% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2024Quote: ... A 1:1 mixture of MEM non-essential amino acids (100x) and MEM amino acids (50x) without L-glutamine (Sigma-Aldrich) was applied to the surface of 3.5 cm NGM plates ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Microbiology 2023Quote: ... 0.070 g/l ZnSO4·7H2O (0.243 mM) (Riedel-de Haen, Seelze, Germany) and 0.143 g/l CoCl2·6H2O (0.603 mM) (Sigma-Aldrich, St. Louis, USA) and the pH was adjusted to 2.0 with concentrated sulfuric acid ...
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bioRxiv - Synthetic Biology 2024Quote: ... PXB mice were given irradiated PROLAB ISOPRO RMH 3000 feed and drinking water supplemented with L-Ascorbic acid (Cat # 25564, Sigma-Aldrich) at 0.3 mg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... diluted in DMEM without FBS and red phenol supplemented with 2 mM L-glutamine and 25 µM verapamil (V4629, CAS 152-11-4, Sigma Aldrich) for 1 h at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... The embryos were fixed in 100% EtOH at room temperature overnight and then stained in Alcian Blue (150 mg/l Alcian Blue 8GX (Sigma-Aldrich) ON at room temperature ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Eggs used for RNA extractions and HCR in situ hybridisation experiments were collected from mouthbrooding females immediately after fertilisation and then reared individually in 1 mg/L of methylene blue (Sigma Aldrich) in water in 6-well plates (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Acetaldehyde content was further quantified using the supernatant of WT and ΔmftG grown in HdB-Tyl supplemented with 10 g L−1 ethanol alone and 10 g L−1 glucose combined with 10 g L−1 ethanol for 48 h as described in the acetaldehyde Assay Kit (Sigma-Aldrich) by the manufacturers.
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bioRxiv - Bioengineering 2023Quote: ... The composite polymer was prepared using poly(L-lactide) (average Mn 40000) (from Aldrich Chemistry) and polycaprolactone (average Mn 80000) (from Sigma-Aldrich), with a ratio of 4:1 (w/w) ...
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bioRxiv - Cell Biology 2024Quote: ... Purified populations were cultured (at 37°C, 5% CO2) in T cell medium: RPMI-1640 containing HEPES and l-glutamine (R5158, Sigma-Aldrich) supplemented with 10% filtered fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
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Formation of a giant unilocular vacuole via macropinocytosis-like process confers anoikis resistancebioRxiv - Cell Biology 2024Quote: ... suspended cells collected at the indicated time points were isolated by centrifugation and rapidly reseeded on poly-L-lysine (Sigma, P4832)–coated coverslips for 3 min before fixation.
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bioRxiv - Cancer Biology 2024Quote: ... lysates from two cortices were plated onto a T75 flask pre-coated with poly-L-lysine (5 mg/ml, Sigma #P1274) in DMEM supplemented with 10% FBS (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... then were incubated with the secondary antibody (Alexa-Fluor®-647-conjugated goat anti-mouse IgG H+L,1:2000 dilution) for 60 min at room temperature and counterstained with DAPI (Sigma-Aldrich) for another 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, 840035), and β-casein from bovine milk (>98% pure, C6905) were purchased from Sigma. BODIPY-TR-C5-ceramide ...
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bioRxiv - Cell Biology 2024Quote: ... ATCC CCL-81) cells were maintained in Dulbecco’s modified Eagle medium (DMEM) containing 4.5 g/L D-glucose (Sigma Aldrich, Bio&Sell) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... demyelination was induced in the ventral white matter funiculus of the thoracic spinal cord (between vertebrae T11-12 or T12-13) by the injection of 1.2 µL of 1% (w/v) L-α-Lysophosphatidylcholine (Lysolecithin; Sigma-Aldrich) under general anaesthesia ...
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bioRxiv - Genomics 2022Quote: ... was determined in Mueller-Hinton agar (for daptomycin, Ca2+ was added to a final of concentration 50 mg/L) (Sigma-Aldrich) in accordance with EUCAST guidelines ...
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bioRxiv - Neuroscience 2023Quote: ... The spinal column was removed and 4-6 lumbar level DRG were dissected and placed onto coverslips coated with Poly-L-Lysine (Sigma, P8920). DRG were allowed to recover for 8 minutes (P4 ...
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bioRxiv - Neuroscience 2023Quote: ... thoracic and lumbar dorsal root ganglia neurons were extracted from E17.5-E18.5 rat embryos and plated on glass coverslip coated with poly-L-ornithine (Sigma-Aldrich, MO, USA) for 4-6 hours before experiments.
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bioRxiv - Neuroscience 2023Quote: ... Patient-derived and previously established NES cells from a male neurotypical donor (Uhlin et al., 2017a) were seeded on 20 µg/ml poly-L-ornithine (Sigma Aldrich), and 1 µg/ml laminin (Sigma Aldrich ...