Labshake search
Citations for Millipore Sigma :
7401 - 7450 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... LdBob promastigotes were routinely maintained at 26 °C in 5% CO2 and cultured in Dulbecco’s Modified Eagle-Leishmania (DME-L) medium supplemented with 5% SerumPlus™ (SAFC BioSciences/Sigma Aldrich, St ...
-
bioRxiv - Biophysics 2020Quote: ... Neurons were plated at a density of 100,000 cell/mL in glass bottom dishes coated with 1 mg/mL poly-L-lysine (Sigma-Aldrich P2636) and 10 μg/mL mouse protein laminin (Thermo Fisher Scientific 23017-015) ...
-
bioRxiv - Cell Biology 2020Quote: ... B16-F1 cells were plated onto glass coverslips pre-coated for 1 hour at room temperature with 25 μg/ml laminin (L-2020; Sigma-Aldrich) in 50 mM Tris ...
-
bioRxiv - Microbiology 2020Quote: ... A volume of 100 μL of inoculum was then added to each well containing 100 μL of two-fold serial dilutions of PZA (Sigma, P7136) ranging from 4 to 512 mg/L in complete Middlebrook 7H9 broth ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were stained for DAPI diluted in water for 2 min and washed 3 times with water before drying cells on poly-L-lysine (Sigma, P8920) coated coverslips at room temperature protected from light ...
-
bioRxiv - Genomics 2020Quote: ... added immediately before use) supplemented with 300ug/mL of Lysolethicin (L-α-Lysophosphatidylcholine from bovine brain, Sigma, stock at 5mg/mL) and incubated at 4°C overnight ...
-
bioRxiv - Neuroscience 2020Quote: ... mixed together in a ratio of 10,000 GABAergic neurons:10,000 Glutamatergic neurons:5000 astrocytes directly out of thaw onto 96 well imaging plates coated with Poly-L-ornithine (Sigma-Aldrich) at 2 μg/cm2 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane (Sigma-Aldrich ...
-
bioRxiv - Physiology 2020Quote: ... Culture flasks were previously coated with a 2 mg/l porcine gelatin solution (90–110 g, Bloom; Sigma-Aldrich, Dorset, UK) to support cell adhesion.
-
bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were cultured in serum-free and phenol red-free media containing 4.5 g/L glucose (Sigma-Aldrich, Dorset, UK), 1% penicillin/streptomycin ...
-
bioRxiv - Immunology 2020Quote: ... PBMCs were thawed at 37°C in complete RPMI-1640 media (supplemented with 10% FBS, L-glutamine, and Penicillin-Streptomycin-Amphotericin; RP10) containing benzonase (EMD Millipore). NK cells were purified by magnetic bead isolation via negative selection according to the manufacturer’s specifications (Miltenyi ...
-
bioRxiv - Neuroscience 2021Quote: The channels within the chambers of the microfluidic device were then coated with 0.1 mg/mL poly-L-lysine (Sigma Aldrich, USA) and incubated for 24 hours at 37 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 µL of culture for each strain and dilution were plated to LB or LB supplemented with L-Rhamnose (Sigma-Aldrich) to a final concentration of 1% ...
-
bioRxiv - Bioengineering 2021Quote: ... The plating channels and the deposition chambers of the microfluidic devices were then coated using 0.1 mg/mL poly-L-lysine (Sigma Aldrich, USA) and placed in an incubator ...
-
bioRxiv - Genomics 2022Quote: Drosophila S2 cells were obtained from Invitrogen (10831-014) and were cultured in HyClone SFX-Insect cell culture media (HyClone) supplemented with 18 mM L-Glutamine (Sigma-Aldrich). S2 cells were maintained at the confluency of 2-10 million cells per mL at 25°C ...
-
bioRxiv - Microbiology 2022Quote: ... 5 μL was used to inoculate 200 μL of LB broth containing 5 μg/mL of tobramycin in a well of a 96-well plate (Corning, Sigma-Aldrich). An additional 5 μL was added to a corresponding well of another 96-well plate containing 200 μL of LB broth ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... We recovered transformants in a molten SDCAA agarose gel (1.71 g/L YNB without amino acids and ammonium sulfate (Sigma-Aldrich #Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2022Quote: ... or Forskolin as described above then cells were trypsinized and resuspended in drug containing media at 30,000 cells per well in a XF cell plate coated with 0.05% poly-L-lysine then incubated overnight (Sigma-Aldrich, 4707).
-
bioRxiv - Genetics 2022Quote: 750k low-passage 293FT cells were seeded into each well of a 6-well plate coated with poly-L-ornithine (Sigma, P2533). One day after seeding ...
-
bioRxiv - Genetics 2022Quote: ... coated slides containing 12 wells (Carlson Scientific cat# 101206) were treated by pipetting 30 μl 0.01% poly-L-lysine (Sigma Cat# p4707) into each well and incubating at RT for 15 min ...
-
bioRxiv - Immunology 2022Quote: CD34+ cells from each donor was supplemented with CD34-cells from the same donor upto total 15 × 1e6 cells in total 100µL with cold FACS benzonase buffer (FBB, FACS buffer [PBS supplemented with 5% FBS and 20U/mL sodium heparin(Sigma-Aldrich)] with 0.025U/mL benzonase (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2022Quote: ... PBMCs were thawed at 37°C in complete RPMI-1640 media (supplemented with 10% FBS, L-glutamine, and Penicillin-Streptomycin-Amphotericin; RP10) containing benzonase (EMD Millipore). NK cells and B cells were purified from thawed PBMCs by magnetic bead isolation via negative selection according to the manufacturer’s specifications (Miltenyi ...
-
bioRxiv - Bioengineering 2022Quote: ... the 11-mercaptoundecanoic acid (CAS no: 71310-21-9) and L-Tryptophan (CAS no: 73-22-3) were obtained from Sigma Aldrich. The RBD ...
-
bioRxiv - Cell Biology 2019Quote: ... were fully digested for 24 h at 60 °C in papain digestion solution containing 125 μg/ml of papain with 5 mM L-cysteine-HCl and 5 mM EDTA in 100 mM Na2HPO4 (all from Sigma, USA). The DNA content was measured using the Picogreen assay ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... 50 μl of XTT:PMS (at 1 mg/ml and 1.25 mM, respectively, and combined at 50:1 ratio, Sigma-Aldrich, MO, USA) was added into each well and incubated 4 hours for the measurement of cell viability ...
-
bioRxiv - Microbiology 2019Quote: ... All 78 spiked and control soil microcosms were enriched for 24 h at 30 °C in 45 ml of LB supplemented with ampicillin (10 mg/l, Sigma Aldrich). Five hundred µl aliquots of enrichment broth were sampled before and after the 24-h enrichment step ...
-
bioRxiv - Microbiology 2019Quote: ... The cells were washed and re-suspended in 65 µl of 1x PBS and spread onto a pre-treated poly-L-lysine (Sigma Aldrich) slide and left to settle for 20 min ...
-
bioRxiv - Synthetic Biology 2019Quote: ... At 1 h post inoculation (time 0 in Figure 3) each flask was added with the stated amount of L-arabinose (Millipore Sigma): 0% ...
-
bioRxiv - Cancer Biology 2019Quote: ... KMp48 mice were randomized to receive either normal drinking water or water supplemented with 1 mg/L warfarin (Sigma-Aldrich, A2250) starting one day prior to Myc activation ...
-
bioRxiv - Neuroscience 2019Quote: ... triturated using a 1 ml pipette tip and plated in a droplet of growth medium on a glass coverslip precoated with poly-L-lysin (20 µg/cm²; Sigma-Aldrich) and laminin (4 µg/cm² ...
-
bioRxiv - Cell Biology 2020Quote: ... respectively and 10 ml/l of a antibiotic solution with Penicillin (100 U/ml) and Streptomycin (10 mg/ml) (Sigma-Aldrich). P19 cells were directly obtained from ATCC (Teddington ...
-
bioRxiv - Genetics 2020Quote: ... 15 μ l samples were loaded on a 4-20% SDS-PAGE and blotted onto Immobilon P PVDF membrane (Millipore, IPVH00010). Next blocking with 0.5% blocking reagent (Roche ...
-
bioRxiv - Developmental Biology 2020Quote: ... Each well was filled with medium containing 50% phenol red-free CMRL (PAN-Biotech, Germany) supplemented with 10 mM L/glutamine (Sigma-Aldrich) and 50% Knockout Serum Replacement (Life Technologies ...
-
bioRxiv - Synthetic Biology 2020Quote: ... according to manufacturer’s instructions and supplemented with 20 g/mL histidine and 80mg/L adenine hemisulfate (Sigma-Aldrich, St. Louis, USA)
-
bioRxiv - Cell Biology 2020Quote: ... HEK293T cells were plated overnight at 1 × 106 cells/well in six-well plates coated with poly-L-lysine (Sigma-Aldrich) and transfected the following day using PEI reagent according to the published optimized procedures69 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Pellets were suspended with 37°C PBS supplemented with 1% fetal calf serum at a concentration ranging from 104 to 105 cells/ml and distributed on Poly-L-Lysine (cat# P4707, Sigma-Aldrich) precoated coverslips for 3 hours at 37°C in 5% CO2 incubator to allow cells to adhere ...
-
bioRxiv - Biochemistry 2021Quote: ... 30 KDa for T-protein and L-protein and 100 KDa for P-protein) were purchased from Millipore (Billerica, MA, USA). Mut Express II Fast Mutagenesis kit V2 was purchased from Vazyme (Jiangsu ...
-
bioRxiv - Neuroscience 2020Quote: GeneSwitch experimental flies (han5304;;elav-GS-GAL4/UAS-Pdfr) were housed after eclosion on normal yeast food containing 0.2g/L RU486 (Sigma, Cat#8046) diluted into 100% ethanol ...
-
bioRxiv - Microbiology 2021Quote: TREx-BCBL-1-RTA cells were seeded onto poly-L-lysine coated coverslips and 2 µg/mL doxycycline hyclate (Sigma-Aldrich) was added 3 hours later ...
-
bioRxiv - Microbiology 2021Quote: ... Saturated seed cultures were diluted into fresh fermentation media: SV2, ISP2 (For 1 L, add 10 g malt extract (Sigma-Aldrich), 4 g yeast extract (BD Biosciences) ...
-
bioRxiv - Biochemistry 2020Quote: ... 200 μL of the NaOH/DMSO slurry was added to the lyophilized N-glycans in addition to 100μL iodomethane (Sigma-Aldrich, #289566) and placed in on a vortex shaker for 20 minutes at room temperature with a microtube cap to prevent the lid from opening due to increased gas pressure ...
-
bioRxiv - Immunology 2020Quote: ... 5 × 106 activated B cells were washed with PBS and re-cultured in glucose-free medium RPMI medium containing 10% dialyzed FBS and uniformly labeled [13C]-glucose (2 g/L; Sigma-Aldrich) for 24 and 48 h ...
-
bioRxiv - Synthetic Biology 2021Quote: ... viral transductions were performed overnight in a 6-well dish using 800 µl of standard ESC medium with 200 µl virus-containing supernatant supplemented with 8 µg/ml polybrene (TR-1003; Sigma-Aldrich). The selection of positive clones was performed by supplementing the culture medium with 1µg/ml puromycin for 3 days ...
-
bioRxiv - Pathology 2020Quote: ... were coated with 50 μL of 0.05% w/v poly – L– lysine in carbonate – bicarbonate buffer (0.05 M carbonate-bicarbonate pH 7.4, Sigma-Aldrich, St. Louis, UK) and incubated for 60 min at room temperature (RT) ...
-
bioRxiv - Genetics 2019Quote: ... Crosslinked cells were lysed for 10 min on ice in 50 μl of lysis buffer (50mM Tris-HCl pH8, 150mM NaCl, 1mM EDTA, 1% SDS) freshly supplemented with proteinase inhibitor (Sigma-Aldrich). IP dilution buffer (20 mM Tris-HCl pH8 ...
-
bioRxiv - Physiology 2020Quote: ... and seeded onto 6-well plates (105 cells/well) in osteogenic medium (50μg/mL L-ascorbic acid-2-phosphate and 5mM β-glycerophosphate) (Sigma, UK) for 24 days ...
-
bioRxiv - Microbiology 2019Quote: ... The animals were euthanized by immersion in overdose 500 mg/ L of MS-222 buffered to pH 7.4 (Sigma– Aldrich, USA).
-
bioRxiv - Microbiology 2020Quote: ... The Human Respiratory syncytial virus (HRSV-A Long strain ATCC-VR26) was produced in LLCMK2 cells (ATCC CCL7) in EMEM supplemented with 2 mM L-glutamine (Sigma Aldrich), penicillin (100 U/mL) ...
-
bioRxiv - Microbiology 2020Quote: ... Strains were grown at 37°C under aeration in Miller Difco Luria-Bertani liquid broth (LB; Becton, Dickinson and Co.) or on LB agar (LBA) containing additional Select agar (15 g L−1, Sigma-Aldrich). For selection of plasmid-carrying strains ...
-
bioRxiv - Microbiology 2021Quote: 10 000 HEK293T and HEK293T-ACE2 plated on plated on glass coverslips pretreated with polylysin poly-L-Lysine solution 0.01% (SIGMA ref P4832) 5 minutes at room temperature ...
-
bioRxiv - Microbiology 2021Quote: For cell imaging 20 000 HEK293T and HEK293T-ACE2 cells were plated on glass coverslips pretreated with poly-L-Lysine solution 0.01% (SIGMA ref P4832) 5 minutes at room temperature ...