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Citations for Millipore Sigma :
7351 - 7400 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the uniform neuroepithelial sheet was broken into clumps of approximately 500 cells/clump and re-plated onto 1X poly-L-ornithine/laminin (Sigma Aldrich) coated dishes in N2B27 medium ...
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bioRxiv - Microbiology 2021Quote: Brugia malayi adults (NIH-FR3) extracted from the Meriones unguiculatus infection system were maintained in daily changes of RPMI 1640 with L-glutamine (Sigma-Aldrich) supplemented with FBS (10 % v/v ...
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bioRxiv - Cell Biology 2021Quote: ... cells were plated on cell culture plates (48-well or 24-well) coated with 0.01 % Poly-L-Ornithine (Sigma-Aldrich, USA) for 1 h at RT and 20 μg/mL Laminin (Roche ...
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bioRxiv - Biophysics 2021Quote: ... The glass surface was blocked prior to GUV addition by incubating the wells with 200 μL 2g/L BSA (Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: HBP inhibition in vivo was achieved by treatment of tumor bearing mice with 3 doses (10 mg/kg) of DON (6-Diazo-5-oxo-L-norleucine, Sigma-Aldrich) every 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... the medium was substituted with myotube differentiation medium consisting of DMEM (high-glucose, 1,500 mg/L NaHCO3, Kohjin Bio, Japan) supplemented with 5% horse serum (HS; Sigma-Aldrich, USA), 10 ng/mL recombinant human insulin-like growth factor 1 (IGF-1 ...
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bioRxiv - Physiology 2021Quote: ... female C57BL/6J mice (age 10-11 weeks) were given 2mg/ml IDO1 inhibitor (1-methyl-L-tryptopham, L1MT; Sigma-Aldrich) in drinking water supplemented with Splenda sweetener (2 sachets/liter ...
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bioRxiv - Biochemistry 2021Quote: ... 0.01 mg aprotinin and 0.1 mM trans-epoxysuccinyl-L-leucyl amido (4-guanidino) butane (E-64) as a protease inhibitors (Sigma Aldrich®) and lysed by sonication (5 pulses for 1 min each ...
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bioRxiv - Bioengineering 2021Quote: Unlabeled poly-L-lysine (molecular weight 15-30 kDa) and fluorescently labelled FITC-poly-L-lysine (molecular weight 15-30 kDa) was purchased from Sigma-Aldrich. Individual nucleotides (ATP and GTP ...
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bioRxiv - Plant Biology 2020Quote: ... tissues were then moved to embryo maturation media (EMM) with 0.05 mg/L imazapyr (IMZ; Sigma Aldrich Chemicals, St. Louis MO) until shoots formed (Supplementary Figures 2A,B) ...
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bioRxiv - Plant Biology 2021Quote: ... (6.7 g/L yeast nitrogen base without amino acids, 20 g/L glucose, appropriate concentration of relevant Yeast Synthetic Drop-out Medium; Sigma-Aldrich) and incubated three days at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then plated at a density of 125 cells/mm2 on coverglasses (#41001112, Glaswarenfabrik Karl Hecht, Germany) coated with poly-L-lysine (# P2636, Sigma, USA) and laminin (#354232 ...
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bioRxiv - Neuroscience 2020Quote: ... and the organ was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore, USA) and laminin (50 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... The middle region of the cochlea was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore #P4957, USA) and laminin (50 μg/mL ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl cells were spread on a 1 mm glass slide pre-treated with 5 μl poly-L-lysine (Sigma Aldrich). A Nikon 9Oi eclipse microscope was used to capture differential interference contrast (DIC ...
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bioRxiv - Microbiology 2021Quote: ... the alumina membranes were washed in sterile dH2O and incubated in 0.1 mg/ml poly-L-lysine (Sigma-Aldrich, Stockholm, Sweden) in dH2O for 5 minutes to prepare the surface for Matrigel coating ...
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bioRxiv - Neuroscience 2020Quote: ... DRG tissues were digested and incubated for 21 minutes at 37°C in a solution containing 1 mg L-cysteine (Sigma-Aldrich), 60U Papain (Worthington Biochemical Corporation) ...
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bioRxiv - Microbiology 2021Quote: Metabolites were extracted from cell pellets through addition of 50 μl chloroform and vigorous vortexing, followed by 200 μl methanol/ultrapure water (3:1, containing 5 μM scyllo-inositol (Sigma I8132) as internal standard ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were washed six times for 15 minutes each in PBS and subsequently immersed in Poly-L-Lysin (Sigma-Aldrich, P4707) for one minute ...
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bioRxiv - Physiology 2020Quote: ... all samples were homogenized in ice-cold lysis buffer (20mM Tris, 150mM NaCl, 0.05% Tween) and 1% each of 1 mmol/l PMSF (Sigma-Aldrich, P7626), 200mM Na3VO4 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Two-fold serial dilutions of an initial concentration of 5 μg/ml of the mAbs were incubated with NDV LaSota or rNDV-H5 for 3h at 37°C in complete medium supplemented with 50 ng/ml L-1-tosylamido-2-phenylethylchloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 50 µL of extract were incubated with 5 µL of β-Glucosidase from almonds (0.5 U/µl in acetate buffer, Sigma-Aldrich) during 90 min at 37°C to release SA from SA-glucoside (SAG) ...
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bioRxiv - Molecular Biology 2020Quote: ... Mice were kept on 10 mg/L 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC; Sigma-Aldrich, Cat. No. PHR1731-1G) in drinking water when indicated ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were recovered by treating with 300 μl (400 µl wounds) or 150 µl (200 µl wounds) of 0.5 mg·ml-1 collagenase type 1 (EMD Millipore Corp, USA) for 1 hour at 37°C to break down the matrix ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with a filter-sterilized solution of dextrose to a final concentration of 1 g/L (where all materials except water were purchased from Sigma-Aldrich). DMM is autoclaved and allowed to cool before use.
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bioRxiv - Molecular Biology 2021Quote: ... VICs were seeded at 1×105 cells/well into 6-well culture plates and stimulated with FeSO4 (10 μmol/L) concentrated with iron(II) sulfate heptahydrate (Sigma, USA) in PBS ...
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bioRxiv - Cancer Biology 2021Quote: Human MDA-MB-231 breast adenocarcinoma cells obtained from ATCC (ATCC HTB-26) were grown in L-15 medium (Sigma-Aldrich) supplemented with 15% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: ... Cellular contractility was modulated by incubating tissues with Oleoyl-L-α-lysophosphatidic acid (LPA) at a final concentration of 20µM (L7260, Sigma-Aldrich), or (−)-Blebbistatin at a final concentration of 50µM (B0560 ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cell suspension was plated onto 22 mm glass coverslips previously coated with poly-L-ornithine (Sigma, mw 100,000 – 200,000), in a plating media consisting of ...
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bioRxiv - Biophysics 2021Quote: ... RBCs were allowed to settle onto poly-L-lysine coated coverslips (squeaky clean coverslips were coated with a 0.1% (w/v) solution of poly-L-lysine (Sigma-Aldrich P8920) 1-2 h room temp with poly-L-Lysine then dried and kept at 4°C until ready for use) ...
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bioRxiv - Cell Biology 2021Quote: ... and cell pellets were lysed for 30 min on ice with 600 µl of Cell Extraction Buffer (Invitrogen, supplemented with 1mM of PMSF and 50 µl/ml of Protease Inhibitor Cocktail, Sigma Aldrich), with vortexing at 10 min intervals ...
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bioRxiv - Cancer Biology 2022Quote: ... The human mesothelial cell line MeT-5A was purchased from ATCC (#CRL-9444) and cultured in Medium 199 containing 1.5 g/L sodium bicarbonate (Sigma-Aldrich #M4530), 10% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... Buffers were made by adjusting Milli-Q water using 5 M NaOH and 80% L-(+)-Lactic acid (27715, Sigma-Aldrich, Germany). Gelatin was prepared by hydrating porcine gelatin (G1890 ...
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bioRxiv - Bioengineering 2022Quote: ... were digested overnight in papain digestion buffer (containing 100 mmol phosphate buffer, 5 mmol L-cystein, 5 mmol EDTA and 140 μg/ml papain (P4762, Sigma-Aldrich)) at 60 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... All cells were cultured in RPMI 1640 medium with L-glutamine and 25 mM Hepes (Sigma Aldrich, St. Louis, MO, USA) and 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... The residual proteins bound to exDNA were digested by 2-h incubation with 0.85 g L−1 proteinase K (Sigma-Aldrich, UK) at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... were cleaned for 30 minutes with argon plasma (PDC-002, Harrick Plasma, Ithaca, NY) and then coated with poly-L-lysine (PLL, 150-300 kDa; P4832; Sigma-Aldrich) for 15 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 150 mg/L sodium 4-methyl-2-oxovalerate (Sigma K0629) ...
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bioRxiv - Biochemistry 2022Quote: ... Dimethyl LV labeling for geminal pair determination used 150 mg/L 2-Keto-(3-methyl-13C)-butyric-4-13C,3-d acid sodium salt (Sigma 589063). Valine only labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887 ...
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bioRxiv - Biochemistry 2022Quote: ... 13C-ILV labeling used 150 mg/L 2-Keto-3-(methyl-d3)-butyric acid-4-13C,3-d sodium salt (Sigma 691887) and 70 mg/L 2-Ketobutryic acid-4-13C,3,3,d2 sodium salt hydrate (Sigma 589276 ...
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bioRxiv - Neuroscience 2022Quote: ... and three old (5.5 months old) Nothobranchius furzeri (GRZ strain) male fish were sedated in 200mg/L of Tricain (Sigma-Aldrich, A5040), and then euthanized in 500mg/L of Tricain in system water ...
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bioRxiv - Neuroscience 2022Quote: ... cells were re-plated onto glass coverslips pre-coated with poly-L-lysine (cat. #P4832, Sigma-Aldrich, St. Louis, MO, USA). After 14-24h ...
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bioRxiv - Neuroscience 2022Quote: 90,000 human NPCs were plated in a well of Chambered Coverglass wells that had been coated with Poly-L-Ornithine (Sigma Aldrich) and Laminin (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... Roots were sliced and placed in a petri dish (Falcon 351008) holding one 70 μm strainer (Falcon 352350) with 7 ml buffer B (cellulase 15 g l−1, (Sigma #C1794) and pectolyase 1 g l−1 ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were then counted and plated at a density of 40000 cells/cm2 on poly-L-lysine (P2636, Sigma-Aldrich) (0.2 mg/ml in borate buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... 1×106 cells were fixed overnight in a solution containing 4% formaldehyde and 4% acrylamide in PBS and adhered to a poly-L-lysine (Sigma, P4707) coated coverslip ...
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bioRxiv - Bioengineering 2022Quote: ... The two-post array supporting the newly formed hydrogels was then lifted out of the casting plate and transferred to a fresh 24-well plate containing Lonza growth medium supplemented with 2 g/L aminocaproic acid (Sigma-Aldrich) to inhibit fibrinolysis ...
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bioRxiv - Developmental Biology 2022Quote: ... Egg chambers were dissected out in the media and moved into cover-glass chambers (Lab-Tek,155411) coated with 0.1 mg/ml poly-L-Lysine (Sigma-Aldrich, P7405) using truncated pipette tips ...
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bioRxiv - Neuroscience 2021Quote: ... for 5 days at 37°C in PTwH buffer (PBS; 0.5% Tween-20, Fisher, BP337; 10mg/L Heparin, Sigma-Aldrich, H3393). After 2 days of washing with PTwH ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington], DNase [Worthington], L-cysteine [Sigma-Aldrich]) for 30 min at 37 °C ...