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Citations for Millipore Sigma :
7301 - 7350 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Briefly, one day before transfection, effector cells (i.e., S-expressing cells) were seeded on the poly-L-lysine (Sigma, Cat# P4832) coated coverslips put in a 12-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... samples were incubated in L-Cry antibodies 5E3-3E6-E8 (1:100) and 4D4-3E12-E7 (1:50) in 5% sheep serum (Sigma-Aldrich). Secondary antibody ...
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bioRxiv - Genomics 2022Quote: ... GFP positive cells were plated at an average density of 0.5 cells per well in a 96-well plate (previously treated with poly-L-Lysine (Sigma #P4707-50ml) to improve cell adhesion ...
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bioRxiv - Cell Biology 2022Quote: ... and the resulting F2 plants were selected on MS plates containing 50 mg/L of kanamycin (Sigma-Aldrich, catalogue number K1377). Because the G2/M-marker CYCB1;1 is strongly expressed during DNA damage46 ...
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bioRxiv - Physiology 2022Quote: ... 117 NaCl, 10 KCl, 1 MgCl2, 10 creatine, 20 taurine, 5 adenosine, 2 L-carnitine, 10 glucose, 10 HEPES [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2021Quote: ... A 12 mm round glass coverslip was brushed with a solution composed of poly-L-lysine hydrobromide (0.078% weight/volume; Sigma Aldrich: P1524) and Kodak Photo-Flo 200 (0.2% volume/volume ...
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bioRxiv - Neuroscience 2021Quote: ... After 24 hours, media was changed to differentiation media (Advanced DMEM/F12 with N2, 2mM L-glutamine, 1mM DbcAMP (Sigma D0627), 1 µg /ml tetracycline (Sigma T7660) ...
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bioRxiv - Neuroscience 2021Quote: ... We use a combination of antibodies (rabbit anti-parvalbumin, 1:5000, PV235, Swant + biotin-conjugated lectin from WFA, 1:250, L-1516, Sigma-Aldrich). On the day after ...
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bioRxiv - Neuroscience 2021Quote: ... Each well of 6-well cell culture treated plates coated with 0.1% Poly-L-ornithine (3 ug/mL; Sigma Aldrich, P4957) were seeded with ∼750K cells and maintained with sterile-filtered neurobasal medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were maintained on cell culture treated multiwell dishes supplemented by coating with 20μg/mL Poly-L-Lysine (Sigma-Aldrich, #9155). Neurobasal Plus + B-27 supplement was used for all experiments (Thermo-Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... An aliquot (20 μL) of the suspension was spread on to a coverslip that had been coated with poly-L-lysine (Sigma, P8920). Cells were fixed with 2% formaldehyde (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Neuronal cultures were maintained in neuronal medium (Neurobasal/B27 medium supplemented with 20 ng/ml BDNF, 10 ng/ml GDNF, both from PreproTech, and 20 ng/ml L-ascorbic acid, Sigma Aldrich).
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bioRxiv - Microbiology 2020Quote: ... measuring the decrease in absorbance at 340 nm 30 min after addition of 5 U of L-lactic dehydrogenase (from rabbit muscle, Sigma-Aldrich) and 4 U of pyruvate kinase (from rabbit muscle ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were incubated in a modified reaction cocktail (209 µL PBS, 5 µL CuSO4, 25 µL 1 M L-ascorbic acid (Sigma, A2174), 1.25 µL AlexaFluor 488 dye ...
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bioRxiv - Biophysics 2020Quote: ... they were mechanically dissociated and plated onto coverslips previously coated with 0.01% poly-L-lisine (Sigma-Aldrich, St. Louis, MO, USA). Five different cell conditions were used ...
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bioRxiv - Genetics 2019Quote: Total RNA was isolated from healthy wandering third instar larvae of wild type and delayed third instar larvae from homozygous l(3)tb mutant using TRIzol reagent following the manufacturer’s recommended protocol (Sigma-Aldrich, India). The samples were incubated with 2U of RNase-free DNaseI (MBI fermentas ...
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bioRxiv - Plant Biology 2019Quote: ... and 0.5% (w/v) bovine serum albumin (Bovogen biologicals BSAS1.0) with addition of 20 mM cysteine-L (Sigma Aldrich A-9165) prior to grinding and pH adjusted to 7.5 ...
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bioRxiv - Plant Biology 2019Quote: ... Washed cells were transferred to heavy media (15N) containing two nitrogen sources for optimal growth (Li et al., 2012) including 1.65 g/L 15NH415NO3 (Sigma Aldrich 299278) and 1.9 g/L K15NO3 (98% 15N Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... The tissue was dissociated by repeated pipetting in Leibovitz’s L-15 medium containing 100U/mL DNase I (Sigma Aldrich, D5025-15KU). Dissociated cells were concentrated via centrifugation (800 rcf for 4 minutes) ...
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bioRxiv - Biophysics 2019Quote: ... 10 µl of lipid mixture was deposited on the conductive face of two Indium Tin Oxide (ITO) slides (purchased from Sigma Aldrich) and left under vacuum for more than 2 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... in standard stroma culture medium (RPMI1640 medium with HEPES modification containing 10% foetal calf serum, 2mM L-glutamine and 1% penicillin and streptomycin (Sigma-Aldrich). On day 2 ...
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bioRxiv - Neuroscience 2019Quote: ... cells at 14 DIV were treated with culture medium containing 100 µg/ml L-ascorbic acid 2-phosphate (vitamin C, Sigma, 49752) for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... and cultured in adult brain slice culture medium containing 840 mg MEM Eagle medium with Hanks salts and 2mM L-glutamine (Sigma, M4642), 18 mg ascorbic acid (Sigma ...
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bioRxiv - Biophysics 2019Quote: ... The other was buffer exchanged into SEC buffer using a PD-10 column (GE Healthcare The protein was then treated with 0.4 units of bovine kidney α-L-fucosidase (Sigma) at 18 °C ...
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bioRxiv - Cell Biology 2019Quote: ... L-lysine-13C6, 15N2 (Lys-8, #608041)) and L-arginine-13C6, 15N4 (Arg-10, #608033) stable isotope labelled amino acids (Sigma-Aldrich). Media was also supplemented with 10% dialysed FCS (Sigma) ...
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bioRxiv - Biochemistry 2019Quote: ... was centrifuged at 3500 rpm for 30 seconds and the pellet resuspended in 30 µl of ookinete medium (RPMI-1640 medium containing L-glutamine (SIGMA, R6504), 0.024 mg/ml gentamycin (HyClone ...
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bioRxiv - Physiology 2019Quote: The thyroid hormone solution (TH solution) was composed of a mix of T4 (L-thyroxine, ≥ 98% HPCL, CAS number 51-48-9, Sigma-Aldrich) and T3 (3,3’,5-triiodo-L-thyronine ...
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bioRxiv - Developmental Biology 2019Quote: ... on gelatin-coated plates and grown for 5 days in the presence/absence of L-Pro (250–500 μM) (Sigma-Aldrich), with a medium change at day 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 μL of DNA plasmids (1 μg/μL per construct) was prepared with 0.5 μL of 2.5% FastGreen (Millipore Sigma cat. #F7252). P0 – P1 mouse pups were anesthetized by cryoanesthesia on ice ...
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bioRxiv - Bioengineering 2021Quote: ... a 95/5 L-lactide/DL-lactide copolymer (Corbion) 2% m v−1 solution in dry 1,4-dioxane (Sigma-Aldrich, 296309) was spin-coated at 3000 rpm for 30 s on 1.25 × 1.25 cm Corning® glass microslides (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... mice were anaesthetised using inhaled isoflurane (4% (v/v) isoflurane and 2 l O2/min) and 30 μl of LPS (250 μg/mL, Sigma-Aldrich) or vehicle PBS were administered intranasally ...
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bioRxiv - Developmental Biology 2020Quote: ... Oocytes were collected by perforating the ampulla using a needle and cultured in a drop of KSOM + 1mM reduced L-glutathione (Millipore MR-020P-5F, Sigma G6013) under mineral oil (Sigma M8410 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10 % Foetal Calf Serum (FCS, LabTech International Ltd, Heathfield, East Sussex, UK) and 4 mM L-glutamine (Sigma-Aldrich).
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bioRxiv - Biophysics 2020Quote: ... Seeds were surface sterilised with 70% ethanol and grown on vertically oriented plates containing 2.2 g L-1 Murashige and Skoog growth medium (Sigma-Aldrich, pH5.7) supplemented with 1 % sucrose and 0.8 % Bacto agar (BD Biosciences) ...
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bioRxiv - Genomics 2020Quote: Zebrafish embryos/larvae were euthanized by prolonged immersion in 200-300 mg/L tricaine (MS222; ethyl-3-aminobenzoate methanesulfonate, #E10521-10G, Sigma-Aldrich). Whenever necessary the chorion was removed ...
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bioRxiv - Microbiology 2021Quote: Streptomyces scabiei 87-22 and its ΔcebR mutant (22) were routinely cultured in Tryptic Soy broth (TSB, 30 g/l, Sigma-Aldrich) or ISP2 (for 1l ...
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bioRxiv - Cell Biology 2020Quote: ... Osteogenic induction was performed in low-glucose DMEM medium supplemented with 50 μg mL−1 L-ascorbic acid 2-phosphate (Sigma-Aldrich), 10 mM glycerol 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... For fixation in solution the cells were directly re-suspended in the fixation solution and transferred to a poly-L-lysine (Sigma, P4707) coated coverslip in a 24-well plate ...
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bioRxiv - Bioengineering 2021Quote: Media for 24-well WormCamp plates is prepared identically to standard NGM plates (Stiernagle 2006) with agar replaced by 25 g/L gellan gum (Gelrite, Sigma Aldrich), which creates a gel with greater optical transparency than agar does ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded on coverslips coated with poly-L-ornithine (0.1 mg/mL in borate buffer) (PLO; Sigma-Aldrich, MO, USA) or culture plates (Corning ...
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bioRxiv - Biophysics 2021Quote: ... spin labels were added to 200 µM final concentration into the same buffer containing 1- or 5 mM L-ascorbic acid (Sigma Aldrich).
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bioRxiv - Bioengineering 2020Quote: ... the chip was taken out of the live-imaging setup and 170 μl of the total volume of the treated chamber was replaced by a solution of Penicillin G sodium salt (Sigma, 13752) with a concentration of 100 mg/ml (equivalent to 2,8 × 104 IU) ...
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bioRxiv - Biochemistry 2020Quote: ... Heparin (20 μl of 50 mg.ml−1) was reacted with 20 μl hydroxylamine-biotin in 40 μl 300 mM aniline (Sigma-Aldrich, UK) and 40 μl 200 mM acetate pH 6 for 48 h at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... and plated on MatTek-brand glass bottom dishes (P35-G.1) prepared appropriately (washes with increasing concentrations of ethanol, followed by coating with poly-L-Lysine (Sigma P4707) for 5 minutes and subsequent washes with water and airdrying for 2h. ...
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bioRxiv - Biochemistry 2020Quote: SV40-immortalized mouse embryonic fibroblasts (MEF, a kind gift of K. L. Rudolph) were grown in high glucose DMEM medium (Sigma, D6429) until they reached 70 % confluency ...
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bioRxiv - Neuroscience 2021Quote: ... in a mixture of two monoclonal antibodies against L-Cry, 5E3-3E6-E8 and 4D4-3E12-E7 (1:100 and 1:50, correspondingly, in 5% sheep serum (Sigma-Aldrich)) (see accompanying manuscript for further details) ...
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bioRxiv - Neuroscience 2021Quote: The plating channels within the chambers of the microfluidic device were then coated with 0.1 mg/mL poly-L-lysine (Sigma Aldrich, USA) for 24 hours in an incubator ...
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bioRxiv - Neuroscience 2020Quote: ... transfected cells were detached by phosphate-buffered saline (PBS) containing 5 mM EDTA and seeded on 12-mm coverslip coated with poly-L-lysine (PLL; Sigma-Aldrich) at 2 x 104 cells/well ...
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bioRxiv - Neuroscience 2021Quote: ... The contribution of L-type voltage gated Ca2+ channel activation to t-LTP was evaluated with bath applied Nifedipine (25 μM, Sigma, Germany). To interfere with group I metabotropic glutamate receptor (mGluR ...
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bioRxiv - Neuroscience 2021Quote: ... Neuronal cultures were maintained in neuronal medium (Neurobasal/ B27 medium supplemented with 20 ng/ml BDNF and 10 ng/ml GDNF, both from PreproTech; and 20 ng/ml L-ascorbic acid, Sigma-Aldrich). MFCs were made with Sylgard 184 silicone elastomer kit (Dow Corning ...