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Citations for Millipore Sigma :
7251 - 7300 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Neuroscience 2024Quote: Mice at 12 weeks of age were euthanized with CO2 gas in accordance with AVMA guidelines and trans-cardially perfused with ice-cold PBS containing heparin at 10,000 units/L (Sigma #3149) for 2 minutes at 6 ml/minute ...
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bioRxiv - Biophysics 2024Quote: ... 0.2Xconcentrated BHI media was obtained by dissolving 7.4 g while a third 1X solution was supplemented by 8 g/L of D-Glucose (Sigma Aldrich).
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bioRxiv - Cancer Biology 2024Quote: ... The cells were further cultured in RPMI-1640 supplemented with 25 mM [U13C]-glucose in the absence or presence of 11 mM L-lactate (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... the cell medium was replaced with a 0.1 mg/mL Poly-L-Ornithine solution (PLO) (Sigma-Aldrich, A-004-C), and the chips were incubated overnight in a fridge at 4 ◦C ...
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bioRxiv - Neuroscience 2024Quote: ... 10,000 cells were plated in NMM on acid-washed coverslips (15 mm; Carolina Biological, Burlington, NC) pre-coated with 100 µg/mL poly-L-lysine (PLL; Sigma) and 10 µg/mL laminin (Invitrogen/Thermo-Fisher).
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bioRxiv - Bioengineering 2024Quote: ... Nutrient agar plate cultures consisting of 28 g L-1 nutrient agar powder (Oxoid) supplemented with 25 µg mL-1 chloramphenicol (Sigma) were incubated at 30 °C for two weeks ...
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bioRxiv - Cell Biology 2024Quote: ... within a monolayer over the course of 21-30 days on 10 μg/mL poly-L-ornithine (P3655 Millipore Sigma) in phosphate buffered saline (PBS ...
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bioRxiv - Biochemistry 2024Quote: ... The cultures were harvested and resuspended in phosphate buffer (PBS) supplemented with 2 mM of Nα-tosyl-l-lysine chloromethyl ketone hydrochloride (Sigma) and protease inhibitor cocktail (Calbiochem ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mL of MSC supplement and 6 mL of LAlanyl-L-Glutamine (ATCC, PCS-500-040) and 0.2 mg/mL G418 (Sigma, G8168) were included as medium supplements ...
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bioRxiv - Biochemistry 2024Quote: ... for 3 hours at 15° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Biochemistry 2024Quote: ... for 30 minutes at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were plated in in 24-or 6-well plates previously coated with Poly-L-ornithin (0,1 mg/ml, Sigma-Aldrich) at a density of 250,000 cells/mL ...
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bioRxiv - Neuroscience 2024Quote: Cells were seeded on glass-coverslips pretreated with poly-L-lysine 50 μg/ml plus laminin 3.3 μg/ml (Sigma-Merck) and fixed with 4% paraformaldehyde before immunolabeling ...
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bioRxiv - Microbiology 2024Quote: ... Cell suspensions were centrifuged at 4415 g for 5 min and washed in physiological water (9 g/L of sodium chloride, Sigma), and suspended in 5 mL of synthetic must for inoculation (either S200 or S280).
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bioRxiv - Immunology 2024Quote: ... with 2% FBS and 20 mM HEPES (Hyclone) and plated onto poly-L-Lysine (PLL; 20 µg/ml, Sigma-Aldrich)-coated 35-mm-diameter culture dishes (MatTek ...
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bioRxiv - Immunology 2024Quote: ... coli at MOI of 100 were added for 2 h to 1×105 neutrophils seeded onto 0.01% poly-l-lysine (Sigma-Aldrich) treated 96-well black cell culture plates with the optical bottom ...
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bioRxiv - Neuroscience 2024Quote: ... and resuspension in re-expressing medium (NeuroBrew-21, 1:50, Cat. No. 130-093-566 and 200 mM L-glutamine, 1:100, Sigma; in MACs Neuro Medium ...
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bioRxiv - Molecular Biology 2024Quote: ... 900 μL of supernatant was then diluted with 8.5 mL of particle-free Dulbecco’s phosphate-buffered saline (DPBS, Sigma-Aldrich, USA) and carefully layered over 2 mL of 20% sucrose (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... The cell medium was then replaced with 0.1 mg/mL Poly-L-Ornithine (PLO) solution (Sigma-Aldrich, A-004-C), and the chips were incubated in a refrigerator at 4 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: HeLa or SH-SY5Y cells were cultured onto 12mm glass coverslips (Thermo-Scientific) coated with Poly-L-Lysine (Sigma-Aldrich). Cells were washed in PBS and fixed with 4% w/v Paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2024Quote: ... 100 µL of overnight cultures grown in M63CA+G was centrifuged and resuspended in 100 µL of PBST (PBS + 0.05% v/v Tween20) containing a 1:100 dilution of mouse monoclonal α-FLAG antibodies (Sigma) and incubated overnight at 4 °C with end-over-end rotation in a microcentrifuge tube ...
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bioRxiv - Molecular Biology 2024Quote: KMS-18 and KMS-18-T21A cell lines were seeded at 1x105 in 200µl medium (RPMI 1640 supplemented with 10% Fetal Bovine Serum, without antibiotics) and mixed with polybrene (16µg/ml, Sigma-Aldrich). Cells were then placed in a 48-well plate (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 hour at 30° C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Plant Biology 2024Quote: ... the calli were moved to callus regeneration media (CRM; per L: 4.43 g of LS, 30 g maltose (Sigma/Merck), 600 μl CuSO4 (1 mg/ml) ...
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bioRxiv - Microbiology 2024Quote: ... The λ -red recombination genes on the pKD46 vector were induced through the addition of L-arabinose (final concentration 10 mM, Sigma) to CFT073/pKD46 cultures for 1.5 hours at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... the dye was removed and replaced with Tyrode’s Salt Solution containing 1 g/L sodium bicarbonate (TSS, Sigma Aldrich T2145). The cells were allowed to equilibrate in TSS for 30 minutes at 37°C and were subsequently subjected to imaging on the TxRed channel using a 30ms exposure and a gain of 1.0 at 10X magnification on a fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Genetics 2024Quote: ... and incubated for 1 hour at 30°C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Plant Biology 2024Quote: ... Plants were subcultured in modified Murashige and Skoog (MS) salts medium (pH 5.6, 0.43% (g/L) MS base salt (Sigma Aldrich M5519), 3% (g/L ...
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bioRxiv - Plant Biology 2024Quote: ... Charcoal-purified and humidified air was pushed at 1 L min-1 and pulled at 0.6 L min-1 through trapping filters (25 mg of 80/100 mesh Hayesep-Q adsorbent, Sigma, Switzerland) for two hours ...
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bioRxiv - Neuroscience 2024Quote: ... The cell suspension was then plated onto a 12-well tissue culture plate (0.5×106 cells/well) coated with Poly-L-lysine (PLL) (0.1mg/ ml Sigma Aldrich, # 2636P) and incubated at 37°C in a humidified atmosphere of 95% oxygen and 5% CO2.
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bioRxiv - Neuroscience 2024Quote: ... the spinal cord was exposed between two vertebrae of the thoracic column and demyelination was induced by injecting 1uL of 1% lysolecithin (L-lysophosphatidylcholine, Sigma) into the ventral funiculus at a rate of approximately 0.5 µl/min−1 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed with 500 µl ice-cold KHB to terminate the uptake and subsequently lysed by adding 200 µl Ultima Gold Scintillation Cocktail (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... and plated in NSM medium w/o bFGF onto plated double-coated with 0.1 mg/ml poly-l-ornithine hydrobromide (Sigma, P3655) in Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Plant Biology 2024Quote: Seedlings were grown in vitro on half strength Murashige and Skoog medium (1/2MS10) (Murashige & Skoog, 1962) containing 2.2 g/l half strength MS medium (Sigma M5519) with 10g/l of sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... trituration was done with a fire-polished Pasteur pipette to obtain a single-cell suspension which was plated on poly-L-lysine coated 60mm dishes (1 mg/mL, Sigma) at a concentration of 200-250 cells/mm2 ...
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bioRxiv - Molecular Biology 2024Quote: ... Shoots were in vitro propagated in MS basal medium with vitamins (M519) supplemented with 30 g/L sucrose (Sigma, S5391), 4.5 µM (1 mg/L ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μL of extract were incubated with 5 μL of β-Glucosidase from almonds (0.5 U/μl in acetate buffer, Sigma-Aldrich) during 90 min at 37°C to release SA from SA-glucoside (SAG) ...
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bioRxiv - Plant Biology 2020Quote: ... M82 and yft1 tomato seeds were sown separately on MS0 solid medium containing 4.41 g/L MS powder (Sigma, CA, USA), 30.00 g/L sucrose ...
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bioRxiv - Biophysics 2021Quote: ... for 15 minutes and then incubated in phosphate-buffered saline (PBS) with 0.1 mg/mL poly-L-lysine (Sigma-Aldrich, P5899) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were washed with RPMI 1640 and then incubated with 20 μg/ml rabbit anti-hamster IgG (H+L) (Sigma-Aldrich) in RPMI 1640 for the indicated times at 37°C ...
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bioRxiv - Genomics 2021Quote: ... in Minimum Essential Medium Eagle culture medium (MEM) with 10% fetal bovine serum (FBS) and 2mM L-glutamine and antibiotic-antimycotic solution (all obtained from Sigma-Aldrich). Vero cells were incubated at 37°C in a humidified 5% CO2 atmosphere until confluency (~8 × 106 cells ...
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bioRxiv - Cancer Biology 2021Quote: 40 μL of each lysate (concentrations adjusted to 1 μg/μL) was treated with 2.4 μL of premixed click chemistry mixture (final concentrations of 100 μM TAMRA-N3 (Sigma-Aldrich, 760757), 1 mM CuSO4 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were grown for 3 days on coverslips until they reached confluence and then 50 ug/ml L-ascorbic acid-2-phosphate (Sigma Aldrich) was added to cultures ...
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bioRxiv - Microbiology 2020Quote: ... without L-glutamine and without phenol red (Biochrom) supplemented with 30 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) (Sigma-Aldrich) with a pH of 7.4 ...
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bioRxiv - Immunology 2020Quote: ... Mice were intranasally inoculated with 50 μl X31 (0.04 haemagglutination units (HAU)) diluted in viral growth medium (VGM; DMEM with 1% bovine serum albumin (Sigma-Aldrich A0336), 10 mM HEPES buffer ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and two 9 cm Petri dishes containing KNOPS medium (Reski and Abel, 1985) supplemented with 0.5 g/l ammonium tartrate dibasic (Sigma-Aldrich Co). Petri dishes were kept at 25°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... following up with a screen in SD-Trp (1.71 g/L Yeast Nitrogen Base Without Amino Acids and Ammonium Sulphate (Sigma-Aldrich, Y1251), 5 g/L ammonium sulfate (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... 100% ethanol was replaced by Alcian Blue solution and samples incubated ove-night (150 mg/L Alcian Blue 8GX (Sigma-Aldrich) in 100% Ethanol and Acetic Acid Glacial) ...