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Citations for Millipore Sigma :
7201 - 7250 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Plants were grown on solid half-strength MS medium (MS/2) supplemented with 0.8g/L agar (Sigma-Aldrich, A1296 #BCBL6182V) and 1% sucrose ...
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bioRxiv - Physiology 2023Quote: HEK293T cells were seeded on day one into 35 mm glass-bottom dishes (Matek) pre-coated with Poly-L-lysine (Sigma). On day three ...
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bioRxiv - Developmental Biology 2023Quote: ... mTeSR1 media was supplemented with 100 nM SiR-actin (to maintain strong actin fluorescence) and 2.5 mM N-acetyl-L-cysteine (NAC, Sigma-Aldrich A9165 ...
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bioRxiv - Microbiology 2023Quote: ... the hydrolysate was suspended in 50 μL and a volume of 0.4 μL of this suspension was loaded onto the MALDI target plate overlaid with 1.2 μL of Norharmane matrix (Sigma-Aldrich) solubilised in chloroform/methanol (90:10 v/v ...
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bioRxiv - Cell Biology 2023Quote: Mettl3flox/flox and Mettl3VilCreERΔ/Δ mice were moved to new cages at 7 weeks of age and given sterile DI water supplemented with 0.5g/L Ampicillin (Sigma A9518), 0.5g/L Neomycin (MP Biomedicals 180610) ...
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bioRxiv - Cell Biology 2023Quote: ... and rotated at 4°C for 45 minutes in Hank’s Buffered Saline Solution with 10 mM EDTA and 1 mM N-Acetyl-L-cysteine (Sigma A9165). Crypts were isolated by scraping with a glass coverslip followed by vortexing and filtering through a 70 uM (small intestine ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was then concentrated to 100-200 μL using centrifugal filter units with a 3-kDa molecular mass cutoff (Amicon Ultra Centrifugal Filters, 4 mL, Merck Millipore) by centrifugation at 5000 g at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The PAO1 ΔoprF+oprF strain containing oprF under an arabinose-inducible promoter was grown in media containing 0.5% L-arabinose (Sigma Aldrich). Unless otherwise noted ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Each sample was lysed in 500 μL of lysis buffer (50 mM HEPES pH 8.0, 2% SDS, 1 mM PMSF, 1x protease inhibitor cocktail (Sigma-Aldrich)) for 20 mins at RT before heating to 99 °C for 5 min ...
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bioRxiv - Microbiology 2023Quote: Fixed isolated biofilms and/or whole C91-PL cells (electroporated with Gag-YFP) plated on 0.01% poly-L-lysine (Sigma-Aldrich) coated fluorodishes were incubated in 50mM NH4Cl for 5min at room temperature (RT ...
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bioRxiv - Microbiology 2023Quote: ... The 6C and 3C mixes were also tested with mucin from porcine stomach type II (0.3 g/L final concentration; Sigma-Aldrich). Heat-stable carbohydrates were also supplemented to basal bYCFA for cultivating pure bacterial strains ...
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bioRxiv - Neuroscience 2023Quote: ... Debris was removed by 2 successive centrifugations (6 minutes at 600 rpm) and cells were plated onto coverslips coated with 0.01% poly-L-ornithine (Sigma, P4957) and incubated overnight at 37 °C with 5% CO2 in serum-free DMEM.
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bioRxiv - Plant Biology 2023Quote: ... The root fresh weight was used to resuspend the samples at a 200 µl / 4.2 g ratio in LC–MS grade methanol with 15 μM internal standard (SI Table 1) (767964; Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: Human Neural Presursor Cells (NPCs, CTL1 S11, Nagy et al. 2017) were plated in Poly-L-Ornithine (PLO) (0.01%, Sigma, #P4957) and Laminin (3μg/cm2 ...
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bioRxiv - Neuroscience 2023Quote: ... Induced neurons were replated onto 6-well tissue culture plates at 450k cells/well on pre-coated with poly-L-orthnithine (Sigma), 4 μg/ml Laminin (Roche Basel ...
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bioRxiv - Molecular Biology 2023Quote: ... Approximately 90% confluent NPCs were dissociated into a single-cell suspension using Accutase and plated on 40 μg/ml Poly-L-ornithine (Sigma) and 20 μg/ml laminin-coated (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... OPCs were plated at a density of 3,000 cells per well in 96-well plates (Falcon) previously coated with poly-L-lysine (10 µg/mL; Sigma-Aldrich) and laminin (10 µg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... tumor tissue was dissociated using digestion buffer (1x PBS with 10 U/ml papain, 200 μg/ml L-cysteine, 250 U/ml DNAse, 1% penicillin-streptomycin, all from Sigma). Cells were triturated and passed through a 100 μm cell strainer to obtain a single-cell suspension ...
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bioRxiv - Plant Biology 2024Quote: ... A single yeast colony was inoculated in 25 ml of selective liquid media {6.7 g/L yeast nitrogen base without aminoacids (Sigma Y0626), 0.64 g/L complete supplement mixture minus tryptophan (CMS-Trp) ...
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bioRxiv - Cell Biology 2024Quote: ... leaf pieces were transferred onto solid (0.8 % phyto agar [Duchefa]) MGI medium (full-strength MS medium containing 1.6 % glucose [Roth], 1 mg/l BA [Sigma-Aldrich] ...
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bioRxiv - Synthetic Biology 2024Quote: ... The natural transformation was started by adding 3.5 µL of the precultures (OD600 ∼ 9 – 11) to 350 µL sea salt medium (28 g/L (Sigma, S9883)) with 100 µM IPTG and 100 ng of tDNA in a 1.5 mL reaction tube ...
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bioRxiv - Neuroscience 2023Quote: ... The recording area of Accura HD-MEA cartridges (3Brain) were coated ON with 100 μg/ml poly-l-lysine (Sigma), washed x 3 with dPBS (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: Each sample was then tagmented by mixing 2 μL of 0.5 ng/μL cDNA, 4 μL of 2X TD buffer (20 mM Tris pH 8.0, 10 mM MgCl2, 20% dimethylformamide (Sigma, D4551)) ...
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bioRxiv - Neuroscience 2024Quote: 4 µL of the SP3 bead-slurry was added to 25 µL of each sample (if samples had less volume, volume was adapted to 25µL by the addition of Millipore water). Subsequently 29 µL acetonitrile were added ...
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bioRxiv - Molecular Biology 2024Quote: ... The small cultures were transferred to 2 L Terrific Broth (TB) medium supplied with 0.05% antifoam SE-15 (Sigma Aldrich) and 50 μg/ml kanamycin ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were grown on Nunc multidishes or in T25 flasks pre-coated with Poly L-Ornithine (15 μg/ml, Sigma) and Laminin (2 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... containing solid (7 g L−1 agar) full-strength Hoagland (2.75 mL well−1; pH adjusted to 6.5) (Sigma-Aldrich, Merck), sealed with parafilm and placed under the growth chamber ...
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bioRxiv - Microbiology 2023Quote: ... Human neuroblastoma cell line SK-N-SH (ATCC HTB-11) was cultured in Dulbecco’s Modified Eagle’s Medium with 1g/L glucose (Sigma-Aldrich, FIN) supplied with 10 % FBS ...
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bioRxiv - Microbiology 2023Quote: ... human lung epithelial cell line A549 and A549 cells expressing ACE2 and TMPRSS2 (AAT) were cultured in Dulbecco’s Modified Eagle’s Medium with 4500 mg/L glucose (Sigma-Aldrich, FIN) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2023Quote: Alcian Blue staining was performed using a staining solution containing 400 mg L-1 of Alcian Blue (8GX, Sigma-Aldrich). The solution was prepared in acidified ultrapure water ...
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bioRxiv - Neuroscience 2023Quote: ... Germany) with a rounded tip coated with poly-L-Lysine (0.1% wt/vol, in deionized water; Sigma, St Louis MO). The intraluminal suture was secured with a ligature and remained in place until sacrifice 2 ...
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Volumetric Compression Shifts Rho GTPase Balance and Induces Mechanobiological Cell State TransitionbioRxiv - Biophysics 2023Quote: ... 15 μL of the final mix was dispensed on the silanized glass surface and rapidly covered by a dichlorodymethyl silane (Sigma)-treated square coverslip (12mm×12mm) ...
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bioRxiv - Bioengineering 2023Quote: ... sulfo-succinimidyl-4-(N-maleimidomethyl)cyclohexane-l-carboxylate (sulfo-SMCC, Sigma, at a concentration of 2 mg/mL in PBS) were added to the emulsion and kept to react for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... All the cell lines used were maintained in fibroblast medium: DMEM (Dulbecco’s Modified Eagle Medium) with high concentration of glucose (4500 mg/L) (Sigma-Aldrich), 10% FBS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... for immature culture conditions or to maturation culture conditions (DMEM containing low glucose (2 g/L) with Palmitic acid (200 uM, Sigma), Dexamethasone (100 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... and the cells were plated at a density of 75,000 per well of 13 mm diameter onto glass coverslips previously coated with poly-L-lysine (10 µg/mL, Sigma). Cultures were maintained in neurobasal medium supplemented with B-27 ...
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bioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in Borate buffer (1.24 g boric acid (Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... and plated in 24-well plates containing 12-mm glass coverslips pretreated with 50 µg/ml poly-L-lysine (Sigma) in Borate buffer (1.24 g boric acid [Fisher] ...
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bioRxiv - Systems Biology 2024Quote: ... Sorted single and ten cells were directly collected in 2 µL of lysis buffer (50 mM TEAB, pH 8.5 + 0.025% (w/v) n-dodecyl-β-D-maltoside (DDM) (Sigma-Aldrich)) per well ...
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bioRxiv - Cell Biology 2024Quote: ... the neurons were cultured for 48 hours on plastic dishes coated with either poly-L-lysine (Sigma Aldrich, P2636-25MG) for hippocampal neurons or poly-D-lysine for SCG neurons before undergoing replating ...
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bioRxiv - Biochemistry 2023Quote: ... Lactate production was enzymatically quantified as NADH fluorescence (360 nm/460 nm) by the reverse reaction of L-lactate dehydrogenase (Rabbit muscle, L25005KU, Sigma) in a reaction containing 20 µL cell media ...
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bioRxiv - Plant Biology 2024Quote: ... Plants were subcultured in modified Murashige and Skoog (MS) salts medium (pH 5.6, 0.43% (g/L) MS base salt (Sigma Aldrich M5519), 3% (g/L ...
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bioRxiv - Cell Biology 2024Quote: ... the dye was removed and replaced with Tyrode’s Salt Solution containing 1 g/L sodium bicarbonate (TSS, Sigma Aldrich T2145). The cells were allowed to equilibrate in TSS for 30 minutes at 37°C and were subsequently subjected to imaging on the TxRed channel using a 30ms exposure and a gain of 1.0 at 10X magnification on a fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Plant Biology 2024Quote: ... the calli were moved to callus regeneration media (CRM; per L: 4.43 g of LS, 30 g maltose (Sigma/Merck), 600 μl CuSO4 (1 mg/ml) ...
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bioRxiv - Genetics 2024Quote: ... and incubated for 1 hour at 30°C following addition of 10 µM 9:1 S-adenosyl-L-methionine (SAM) p-toluenesulfonate salt (Sigma) to S-adenosyl-L-[methyl-3H]-methionine ([methyl-3H]-SAM ...
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bioRxiv - Neuroscience 2024Quote: ... trituration was done with a fire-polished Pasteur pipette to obtain a single-cell suspension which was plated on poly-L-lysine coated 60mm dishes (1 mg/mL, Sigma) at a concentration of 200-250 cells/mm2 ...
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bioRxiv - Plant Biology 2024Quote: Seedlings were grown in vitro on half strength Murashige and Skoog medium (1/2MS10) (Murashige & Skoog, 1962) containing 2.2 g/l half strength MS medium (Sigma M5519) with 10g/l of sucrose ...
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bioRxiv - Microbiology 2024Quote: ... The λ -red recombination genes on the pKD46 vector were induced through the addition of L-arabinose (final concentration 10 mM, Sigma) to CFT073/pKD46 cultures for 1.5 hours at 30°C ...
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bioRxiv - Neuroscience 2024Quote: ... The cell suspension was then plated onto a 12-well tissue culture plate (0.5×106 cells/well) coated with Poly-L-lysine (PLL) (0.1mg/ ml Sigma Aldrich, # 2636P) and incubated at 37°C in a humidified atmosphere of 95% oxygen and 5% CO2.