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Citations for Millipore Sigma :
7151 - 7200 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: OPCs were seeded at a density of 40,000 cells per well in 96-well plates coated with in poly-L-ornithine (Sigma, P3655) and laminin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... animals were injected with N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT) (Sigma, Cat no. D5942) (10 mg/kg/day ...
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bioRxiv - Cancer Biology 2023Quote: ... fixed cells were pelleted and washed twice with PBS and resuspended in 300 μL hypotonic buffer (1 g/L sodium citrate buffer, 0.1% Triton-X 100) supplemented with 40 μg/mL propidium iodide (Sigma-Aldrich) and 100 μg/mL of RNase A (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich), 1 g/L monosodium glutamate (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μg/ml bovine insulin, 5 μg/ml transferrin, 0.03 nM sodium selenite, 30 nM L-thyroxine; all from Sigma-Aldrich), with one medium change 48 hours after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... adult individuals were sprayed with 1 ul of 1000 mg/L PBO solution (PBO 90% technical grade, Sigma-Aldrich, 291102) on the dorsal thorax ...
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bioRxiv - Bioengineering 2023Quote: ... The cocoons were then degummed by boiling in 1.5 L ultra-pure water (UPW) containing 0.02 M Na2CO3 (Sigma-Aldrich, S7795) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... in 300μl of Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, GibcoTM) + 5 % goat bovine serum (GBS, Sigma Aldrich) + 1% penicillin-streptomycin (10 000 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... L (+) lactic acid production in the lung tissue and BAL was also measured using a Lactate Colorimetric Assay (MAK064, Sigma).
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bioRxiv - Microbiology 2022Quote: ... Biological ammonia oxidation was suppressed by overnight incubation at 50 °C with 0.05 g penicillin g L-1 (Sigma Aldrich) (H ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μL of concentrated virus were added in 2 mL of medium in presence of 8 μg/mL of polybrene (Sigma) for a 60-mm plate ...
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bioRxiv - Microbiology 2023Quote: ... Approximately 1 mL nitrate stock solution (10 g N L-1) containing ∼1% 15N-labelled sodium nitrate (98 atom % 15N, Sigma) was added to achieve a concentration of ∼20 mg N L-1 ...
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bioRxiv - Microbiology 2023Quote: ... followed by an introduction of 0.12 mL nitrate stock solution (40 g N L-1) which contained ∼1% 15N-labelled NO3- (98 atom % 15N, Sigma). The butane culture was flushed with argon gas (99.99% ...
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bioRxiv - Microbiology 2023Quote: ... Next, 1ml of cell maintenance media (1X MEM [GIBCO], supplemented with earl salt, 2% of 200Mm L-glutamine (Sigma-Aldrich), and 2% penicillin-streptomycin solution containing 10000unit/ml penicillin and 10000ug/ml streptomycin (GIBCO ...
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bioRxiv - Synthetic Biology 2023Quote: ... were pipetted into a clean 1.5 mL glass vial and mixed with 25 µL of the Inside-solution (I-solution, 3.2 µg mL−1 ciprofloxacin and 100 µM dextran (50 kDa, Sigma-Aldrich) in PBM9 (glucose replaced with 0.333% w/v sucrose) ...
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The Single-Cell Transcriptome Program of Nodule Development Cellular Lineages in Medicago truncatulabioRxiv - Plant Biology 2023Quote: ... Root segments were moved to a glass plate with 200 μL of modified Nuclei Isolation Buffer (NIB; Conde et al., 2021) containing 0.5 U/mL Protector RNase Inhibitor (Sigma Aldrich). After samples were fragmented with a sterile razor blade ...
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bioRxiv - Pathology 2023Quote: The on-chip FFPE samples were manually processed as follows (see Supplementary Information S5): the photonic chips were first coated with 0.1 % poly-L-lysine (P8920, Sigma-Aldrich) and then glued to conventional glass slides for easier handling using picodent twinsil dental glue (1300-1000 ...
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bioRxiv - Neuroscience 2023Quote: ... that has been pre-treated with 5 M hydrochloric acid and double coated with both 0.01% poly-L-ornithine hydrobromide (Sigma-Aldrich) and GeltrexTM ...
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bioRxiv - Biophysics 2023Quote: ... 0.16–0.19 mm) were coated with 0.01% (w/v) poly-L-lysine (molecular weight [MW] 150,000–300,000 Da, Sigma-Aldrich, Munich, Germany) for four hours at 37°C and rinsed three times with Dulbecco’s phosphate-buffered saline containing Mg2+/Ca2+ (DPBS+/+ ...
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bioRxiv - Cancer Biology 2023Quote: Primary MEFs were derived from wild-type and Casp2−/− embryos at day 13.5 as previously described (10, 27, 39) and maintained in high-glucose DMEM with 0.2 mM L-glutamine (Sigma-Aldrich), 10% FBS (JRH Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a 50% mix of L-WRN fibroblast cell line conditioned media (CM) and primary culture medium (Advanced DMEM/F-12; Sigma) both supplemented with 20% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The edges of the ovarian lobes were collected from anesthetized Xenopus females (30 minutes bath in 1 g/l MS222 (Sigma). To obtain defolliculated fully-grown oocytes ...
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bioRxiv - Neuroscience 2023Quote: ... dissociated hippocampal neurons were seeded onto 18-mm or 25-mm coverslips (Carolina Biologicals) coated with poly-L-lysine (1 mg/ml, Sigma) at a density of 25-40 × 103 cells/cm2 in Neurobasal media (Gibco ...
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bioRxiv - Neuroscience 2023Quote: Mice at 12 weeks of age were euthanized with CO2 gas in accordance with AVMA guidelines and trans-cardially perfused with ice-cold PBS containing heparin at 10,000 units/L (Sigma #3149) for 2 minutes at 6 ml/minute ...
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bioRxiv - Molecular Biology 2023Quote: ... The 30 - 40% interface band was extracted using a needle and syringe (B.Braun, Melsungen, Germany) and dialyzed twice 1:1000 against aqueous sea salt solution (40 g L-1 Sigma Aldrich) in dialysis sleeves (15 kDa MWCO ...
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bioRxiv - Microbiology 2023Quote: ... one portion of the sample was dosed with 10 mg/L of benzalkonium chloride (BAC, ≥95.0% Fluka 12060, Sigma Aldrich), divided into three replicas of 0.15 L ...
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bioRxiv - Microbiology 2023Quote: ... parasites were cultured within O+ human erythrocytes at 5% haematocrit in RPMI 1640 complete medium containing L-glutamine and 25 mM HEPES (Sigma), supplemented with 0.2% sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... Agonist or vehicle was added to cells grown in 12-well plates coated with 0.001% (w/v) poly-L-lysine (Millipore Sigma) and incubated at 37 °C and 5% CO2 as indicated in figure legends ...
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bioRxiv - Bioengineering 2023Quote: ... and 0 µl of concentrated lentiviral particles were reverse transduced into 5×104 HEK293T cells with 8μg/mL polybrene (Millipore-Sigma, TR1003G) in 24 well format with media exchanged after 14 hours of transduction ...
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bioRxiv - Systems Biology 2023Quote: ... Media was prepared as described by Verduyn et al.34 and supplemented with 10 mM glucose as carbon source and additional 0.2 g/L of antifoam C (Sigma-Aldrich). Dissolved oxygen concentration was maintained above 40% throughout the cultivation ...
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bioRxiv - Bioengineering 2022Quote: ... and tumor target (T) cells were co-cultured in triplicates at the indicated E/T ratio using flat bottom poly-L-ornithine (Sigma) coated 96-well plates with 1 × 104 target cells in a total volume of 200 µL per well in NALM-6 medium ...
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bioRxiv - Cancer Biology 2023Quote: ... 300,000 neurons were plated onto circular glass coverslips (Electron Microscopy Services) pre-treated for 20min at 37°C with poly-L-lysine (Sigma) and then 3 hours at 37°C with 5 μg/mL mouse laminin (Thermo Fisher) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with pre-warmed medium three times and cultured in fresh medium supplemented with 2 μg/ml doxycycline and 10 μM S-Trityl-L-cysteine (STLC; Sigma) for 14-16 hours to enrich mitotic cells30 ...
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bioRxiv - Cell Biology 2023Quote: ... composed of Egg yolk L-α-Phosphatidylcholine (EyPC, Avanti Polar), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS, Avanti Polar) and Cholesterol (Sigma) (50:20:30 ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 x 103 cells were seeded on a PLL coated (25 μg/ml Poly-L-lysine hydrobromide (Sigma-Aldrich: P1274)) Seahorse plate ...
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bioRxiv - Cell Biology 2023Quote: ... cells were grown in complete medium and for days 8-14 this medium was supplemented with 50μM L-ascorbic acid (Sigma-Aldrich). Medium changes were performed every 3 days ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl,1 mM EDTA and NP40 1% v/v) supplemented with protease inhibitor cocktail l% v/v (Sigma) and quantified by Bradford assay ...
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bioRxiv - Developmental Biology 2023Quote: ... During live imaging embryos and larvae were immobilized in 0.8% low melting agarose and anesthetized with Tricaine (164 mg/L; MS-222, Sigma-Aldrich) dissolved in E3/PTU.
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bioRxiv - Developmental Biology 2023Quote: ESCs were differentiated to EPL cells by culturing 20,000 cells/cm2 in EPL cell medium (90% DMEM, 10% FBS, Pen/Strep, 0.1 mM β-Me, 330 U/mL LIF, 400 μM L-proline, Sigma) for 6 days ...
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bioRxiv - Immunology 2023Quote: ... Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin) and 50 nmol/L of rapamycin (Sigma- Aldrich), whereas Tconv cultures contained 100 U/mL of IL-2 ...
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bioRxiv - Microbiology 2023Quote: ... Hemin (0.5 mL/L of a 10 mg/mL stock which was prepared in 0.1 N NaOH; Sigma, Cat. # 51280). All solutions were made in Milli-Q-water unless otherwise noted ...
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bioRxiv - Biochemistry 2023Quote: ... Twenty μl of each sample were diluted 14-fold in HDX-MS buffer made with 99.9% D2O (151882-100G, Sigma-Aldrich) to mimic the exposure conditions for HDX-MS experiments ...
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bioRxiv - Biochemistry 2022Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... TCs and platelets suspensions were placed on p24 well plates containing glass coverslips previously treated with poly-L-lysine solution (Sigma). After TC-platelet suspension sedimentation (30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: Cells were seeded onto an XF96 cell culture microplate coated with 0.001% (w/v) poly-L-lysin (Sigma-Aldrich P4707) at 30,000 cells/well and incubated for 48 hrs in glucose-based media supplemented with 0.25 μg/ml doxycycline ...
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bioRxiv - Plant Biology 2023Quote: ... Plants were grown on solid half-strength MS medium (MS/2) supplemented with 0.8g/L agar (Sigma-Aldrich, A1296 #BCBL6182V) and 1% sucrose ...
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bioRxiv - Physiology 2023Quote: HEK293T cells were seeded on day one into 35 mm glass-bottom dishes (Matek) pre-coated with Poly-L-lysine (Sigma). On day three ...
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bioRxiv - Developmental Biology 2023Quote: ... mTeSR1 media was supplemented with 100 nM SiR-actin (to maintain strong actin fluorescence) and 2.5 mM N-acetyl-L-cysteine (NAC, Sigma-Aldrich A9165 ...
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bioRxiv - Microbiology 2023Quote: ... the hydrolysate was suspended in 50 μL and a volume of 0.4 μL of this suspension was loaded onto the MALDI target plate overlaid with 1.2 μL of Norharmane matrix (Sigma-Aldrich) solubilised in chloroform/methanol (90:10 v/v ...
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bioRxiv - Cell Biology 2023Quote: Mettl3flox/flox and Mettl3VilCreERΔ/Δ mice were moved to new cages at 7 weeks of age and given sterile DI water supplemented with 0.5g/L Ampicillin (Sigma A9518), 0.5g/L Neomycin (MP Biomedicals 180610) ...