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Citations for Millipore Sigma :
7101 - 7150 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The cellular suspension was spun at 1800 rpm for 9 min at room temperature and the pellet was resuspended and plated on poly-l-lysine (0.3mg/mL; Sigma Aldrich) coated flasks with DMEM/F12 mixed cell culture medium (F12 media ...
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bioRxiv - Neuroscience 2022Quote: For differentiation of OPC to OL: OPC medium was supplemented with 40 ng ml−1 3,3ʹ,5-Triiodo-L-thyronine (T3) (Sigma; T6397)52 ...
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bioRxiv - Plant Biology 2023Quote: ... 0.5 mM luminol probe 8-amino-5-chloro-7-phenyl-pyrido[3,4-d]pyridazine-1,4(2H,3H)dione (L-012) (Wako Chemicals, Richmond, Virginia, USA) and 20 μg/mL horseradish peroxidase Type VI-A (Sigma, Saint Louis ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Immunology 2023Quote: ... Spores were collected into sterile water with 0.01% Tween by scraping using a L spreader and were filtered through sterile miracloth (Sigma-Aldrich) into a 50 mL centrifuge tube ...
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bioRxiv - Neuroscience 2022Quote: ... Cleaned and UV sterilized MEAs (120MEA100/30iR-ITO arrays; Multi Channel Systems) were coated with 0.1 mg/ml poly-L-lysine (Sigma-Aldrich) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Dissociated spheroids were plated onto culture plates pre-coated with 15 µg/mL Poly-L-Ornithine (PLO, Sigma-Aldrich, P4538), 2 µg/mL fibronectin (FB ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×105 cells were seeded into 12-well plates that had been coated with 1 mg/mL poly-L-ornithine (Sigma) for 60 minutes ...
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bioRxiv - Neuroscience 2022Quote: The mDA neurons from three independent hiPSC lines were at 3 and 4 weeks of differentiation were incubated in phenol-free “N2B27” medium for 24 hours with and without the presence of 80μM L-Dopa (Sigma D9628). For extracellular metabolites ...
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bioRxiv - Plant Biology 2022Quote: ... Sections of 7 to 8 mm were then transferred to slides treated with 100 mg/mL poly-L-Lys (Sigma) and dried on a warm plate at 40 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... Dried samples were then resuspended in 150 µL methanol containing internal standards (15 µM U-13C, 15N amino acid mix, 5 µg/mL 13C2, 15N3 cytosine (Sigma), 10 µg/mL U-13C mannitol and trehalose (Omicron) ...
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bioRxiv - Neuroscience 2022Quote: ... The L-15 medium in the bath was supplemented with 100 μM of FFA or 1 mM Octanol (Sigma-Aldrich). Voltage ramps from −90 mV to +90 mV over 300 ms were applied to study the current-voltage relationships before ...
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bioRxiv - Immunology 2022Quote: ... several colonies were picked and grown anaerobically in standing cultures of brain-heart infusion supplemented media (BHIS: 37 g/L BHI (Sigma); 1 g/L-cysteine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: ... Expanded samples were immobilized on 25 mm (No. 1.5H) coverslips covered with 0.01 % (w/v) poly-L-lysin (Sigma-Aldrich, P8920) and imaged.
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bioRxiv - Cell Biology 2024Quote: Slides for the squashing technique: the slides are washed in a 1:1 ethanol-acetone solution and covered with poly-L-lysine (Sigma) to promote cell adhesion.
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bioRxiv - Bioengineering 2024Quote: ... the wells were next filled with 1 mL of a 0.5 mg/mL poly-l-lysine (MW = 150000 kDa - 300000 kDa, Sigma Aldrich) solution in MilliQ water ...
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bioRxiv - Neuroscience 2024Quote: ... the distilled (DI) water in the devices was replaced by Poly-L-Ornithine solution (PLO) (Sigma-Aldrich, A-004-C) at a concentration of 0.1 mg/mL and incubated at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were then treated with 50 nM TMR-conjugated Halo ligand in growth media growth media (DMEM with 10% FBS, 4.5 g/l Glucose (Sigma, G8769), 1x GlutaMAXTM (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... and PI(4,5)P2 (L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine) ammonium salt) (catalog no. 840046P-1MG) were purchased from Sigma Aldrich/Merck (manufactured by Avanti Polar Lipids ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μL cDNA template at a concentration of 10 ng μL-1 and 3.5 μL of nuclease-free water (Sigma-Aldrich). No template controls were included for all reactions ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 mM/L β-mercaptoethanol and 10 mM nicotinamide and 10 nM exendin-4 (Cat no: E7144 Sigma-Aldrich, USA) until day 28 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 9-15c cell lines were cultured in RPMI-1640 with 2 mM L-glutamine (Sigma-Aldrich; St. Louis, MO), supplemented with 100 U/mL penicillin/100 μg/mL streptomycin (P/S ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse NPCs from E13.5 WT and PlyA-KI forebrain were dissociated and cultured in serum-free N-2/B-27/DMEM medium with 10 ng/ml FGF2 (Life Tech) in poly-L-lysine (PLL, Sigma)-coated 6 well culture plates (Costar ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were incubated for 30 minutes with 3 µmol/L 4,5-diaminofluorescein diacetate (DAF-2 DA; Sigma-Aldrich, D225). After that ...
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bioRxiv - Neuroscience 2024Quote: ... were seeded at 1x105 cells/well in a flat-bottom 96-well plate coated with poly-L-lysine (0.01%, Sigma-Aldrich). Cells were grown in microglia media (10% fetal bovine serum ...
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bioRxiv - Neuroscience 2024Quote: ... and reseeded at lower density in 12 mm diameter glass coverslips previously treated with poly-L lysine (0.01%, Sigma-Aldrich).
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bioRxiv - Neuroscience 2024Quote: ... cells were detached from the Matrigel coated plate using ReLeSR™ and transferred onto poly-L-ornithine (PLO; Sigma; P3655)/Biolaminin coated 12-well plates and cultured in NIM supplemented with RevitaCell™ ...
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bioRxiv - Neuroscience 2023Quote: ... and plated into 12.5 cm2 culture flasks pre-coated with 0.05mg/ml poly-L-lysine (PLL, Sigma, St. Louis, MO). Cells were maintained at 37°C for 5-8 days and D/F-FBS-P/S medium was changed every 2 days.
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bioRxiv - Microbiology 2024Quote: ... GS1783 bacteria were grown in LB broth (Lennox) (Carl Roth, Karlsruhe, Germany) containing 5 g/L of NaCl (Sigma-Aldrich). Lentiviral vector plasmids encoding DSP1–7 or DSP8–11 and their helper plasmids pMD-G and pCMVR8.91 were kindly provided by Dalan Bailey (The Pirbright Institute ...
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bioRxiv - Cell Biology 2024Quote: ... 1x Insulin-Transferrin-Selenium-A and 2 mM L-glutamine] supplemented with 1 μg/mL doxycycline (DOX; Sigma-Aldrich, # D9891), 10 μM SB431542 and 0.1 μM LDN193189 to promote a cortical fate ...
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bioRxiv - Cell Biology 2024Quote: Age and sex matched 9-month-old zebrafish were sacrificed in 1g/l of MS-222 (Sigma Aldrich, MO, USA), fixed in 4% paraformaldehyde in PBS for 72 hour at 4°C ...
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bioRxiv - Immunology 2024Quote: Whole intact tumours were harvested on days 10-13 post engraftment and immediately immersed in fixative solution (1% PFA, 75 mM L-lysine [Sigma, Cat ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were activated as earlier described and adhered to glass slides coated with poly- L-lysine (Sigma Aldrich). The cells were fixed with 4% paraformaldehyde for 15 min at room temperature ...
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FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteriabioRxiv - Microbiology 2024Quote: ... Custom made flow-cell chambers were constructed using slides and cover slips treated with 0.1% poly-L-Lysine53 (Sigma-Aldrich). The slide and cover slip were assembled in the presence of a double layered parafilm as a spacer ...
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bioRxiv - Microbiology 2023Quote: ... Cyst walls were stained with a 1/300 dilution of a biotin-labelled Dolichos biflorus lectin (L-6533, Sigma-Aldrich) for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (SNN1008 ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged L protein mutants were detected using horseradish peroxidase-conjugated anti-FLAG M2 antibody (1:9,000) (A8592; Sigma-Aldrich) and bands were visualised by chemiluminescence using Clarity Max Western ECL Substrate (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and genetically modified MDA-MB 231 cells were cultured at 37 °C and 0 % CO2 in Leibovitz’s L-15 medium (Sigma-Aldrich) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2023Quote: ... The bacmid DNA was isolated from the positive transformants by the alkaline lysis method and transfected into SF9 cells with Escort IV (#L-3287, Sigma) according to the manufacturer’s protocols ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were grown at 30 °C in minimal synthetic media or agar plates containing 6.8 g/L yeast nitrogen base without amino acids (Sigma-Aldrich), 2 g/L dextrose (Formedium) ...
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bioRxiv - Cell Biology 2023Quote: ... An overnight 2xTY starter culture was inoculated into 1L flasks of NH4Cl free M9 medium containing 1.7 g/L of yeast nitrogen base (Sigma Y1251), 1 g/L of 15NH4Cl (Sigma 299251) ...
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bioRxiv - Genomics 2023Quote: ... HaCat cells were cultured in complete HaCat media (DMEM high glucose with L-Glutamine, sodium pyruvate and sodium bicarbonate (Sigma) plus 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 7.4) at 4 L for 15 min and then stained with 20 μg/mL of propidium iodide (Sigma-Aldrich) containing 10 μg/mL of ribonuclease A at room temperature for 30 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich), 1 g/L monosodium glutamate (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 μg/ml bovine insulin, 5 μg/ml transferrin, 0.03 nM sodium selenite, 30 nM L-thyroxine; all from Sigma-Aldrich), with one medium change 48 hours after transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... adult individuals were sprayed with 1 ul of 1000 mg/L PBO solution (PBO 90% technical grade, Sigma-Aldrich, 291102) on the dorsal thorax ...
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bioRxiv - Bioengineering 2023Quote: ... The cocoons were then degummed by boiling in 1.5 L ultra-pure water (UPW) containing 0.02 M Na2CO3 (Sigma-Aldrich, S7795) for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... in 300μl of Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, GibcoTM) + 5 % goat bovine serum (GBS, Sigma Aldrich) + 1% penicillin-streptomycin (10 000 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... L (+) lactic acid production in the lung tissue and BAL was also measured using a Lactate Colorimetric Assay (MAK064, Sigma).
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bioRxiv - Microbiology 2022Quote: ... Biological ammonia oxidation was suppressed by overnight incubation at 50 °C with 0.05 g penicillin g L-1 (Sigma Aldrich) (H ...
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bioRxiv - Developmental Biology 2023Quote: ... 5% CO2 incubator on Corning Primeria or Falcon tissue culture vessels coated with Poly-L-Ornithine (Sigma, cat# P4957-50ML) and Laminin (Sigma ...