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Citations for Millipore Sigma :
7051 - 7100 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 8-12 weeks old mice were used and water was replaced with filtered tap water containing 16 g/L paromomycin sulfate salt (Sigma). During the course of infection ...
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bioRxiv - Microbiology 2020Quote: ... Water and biofilms from the outflow were collected in a sterile 1 L bottle and filtered through a 0.22 μm nitrocellulose membrane (Millipore, USA). The membrane was transported to the laboratory on ice and stored at 4 °C for bacterial enrichment into 10 mL of Nutrient Broth (Biolife ...
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bioRxiv - Microbiology 2019Quote: ... Ambient air was injected in the system at a flowrate of 3 L/min using an aquarium air pump equipped with a 0.2 µm air filter (Millipore, USA). Prior to the start of the experiment ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X cells (2.5×104/well) were seeded in 24-well microplates onto 12-mm cover glasses previously treated with L-polylysine (Sigma) and transiently transfected with pGAE-UFO.664 ...
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bioRxiv - Immunology 2021Quote: ... OAS1 KO 293T cells were seeded in 12 well plates coated with 10 μg/mL poly-L-ornithine hydrobromide (Sigma) and allowed to adhere overnight ...
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bioRxiv - Microbiology 2021Quote: ... Phage 80α stock was diluted in phage buffer (50 mM Tris-HCl pH 7.8, 1 mM MgSO4, 4 mM CaCl2 and 1 g/L gelatin; Sigma–Aldrich), and added to the master mix to reach the desired starting concentration in plaque forming units per mL (pfu/mL) ...
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bioRxiv - Immunology 2021Quote: ... 0.5 µl of an aqueous solution of 15–50 kDa poly(L-glutamic acid) (PGA, Sigma-Aldrich, 100 µg/µl) was mixed with 0.48 µl of synthetic modified sgRNA (2’-O-Methyl at 3 first and last bases ...
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bioRxiv - Microbiology 2021Quote: ... 200 μL of lysis buffer (8M urea in 50mM Tris-Cl pH 7.5, supplemented with phosphatase inhibitor cocktail 3 [Millipore-Sigma]) was added to each of the pellets and pipetted up and down several time to lyse a portion of the pellet ...
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bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
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bioRxiv - Genetics 2020Quote: ... the URA3 gene was cured out by streaking out the selected colonies on SD medium containing (1g/l) 5-Fluoroorotic acid hydrate (Sigma) plates as described previously (Boeke et al. ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Microbiology 2021Quote: Plants cultured with bacteria were washed in PBS and mounted on a microscope slide and covered with a poly-L-Lysine 31 (Sigma)-treated coverslip ...
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bioRxiv - Cancer Biology 2020Quote: ... metabolites were resuspended in 100 μL LC-MS grade water and run on a SeQuant ZIC-pHILIC column (2.1×150 mm, 5 μm, Millipore-Sigma) on a Dionex UltiMate 3000 UPLC using a 0 to 80% gradient of 20 mM ammonium carbonate and 0.1% ammonium hydroxide ...
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bioRxiv - Neuroscience 2020Quote: ... Ganglia were minced using surgical micro scissors and incubated in a papain solution (32 μL papain (27.3 U/mg, no. 3126; Worthington Biochemical, Lakewood, NJ, US and 1 mg l-cysteine, Sigma Aldrich) in 1.5 mL 1X HBSS in a 37°C rocking water bath for 10 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... and the organ was cultured in a well on a cover glass coated with 1:1 poly-L-ornithine (0.01%; Sigma-Millipore, USA) and laminin (50 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... and cultured at 37°C in RPMI-based culture media (10% FBS, L-glutamine, Penicillin/streptomycin, 1X MEM NEAA (Sigma), 1mM sodium pyruvate (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: 100,000 glioma cells were plated onto circular glass coverslips (Electron Microscopy Services) pre-treated for 1 h at 37 °C with poly-L-lysine (Sigma) and then 1 h at 37 °C with 10 µg/ml natural mouse laminin (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... and cultured on glass coverslips (4×104 cells per 18-mm diameter coverslip) (Fisherbrand) coated with poly-L-lysine 10mg/ml (Sigma). Dissociated motor cortex was cultured in SFM [0.034 mg/l bovine serum albumin ...
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bioRxiv - Neuroscience 2022Quote: ... 1-2 mg protein lysate in 750 µl SLB buffer with protease and phosphatase inhibitors was incubated with 2.5-5 µg of anti-Flag antibody (Sigma, # F3165) for 2 hours at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... respectively as described previously except SHH was not used for the maintenance or differentiation of OPCs.25 All OPC and immature oligodendrocyte cultures were maintained on plates or glass coverslips coated with 100 μg/mL poly(L-ornithine) (P3655, Sigma), followed by 10 μg/ml laminin (L2020 ...
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bioRxiv - Neuroscience 2022Quote: ... meninges were removed and individual cortices were transferred to HBSS containing 8 µl Trypsin (25g/L in 0.9 % NaCl) (Sigma, T4549), incubated for 20 min at 37 °C with agitation ...
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bioRxiv - Biochemistry 2022Quote: ... rinsed with isopropanol and water and dried with clean nitrogen gas (Young and Lin, 2018) and coated with 0.01 % Poly-L-lysine solution (Sigma, P4832). Mass calibration was performed using BSA ...
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bioRxiv - Immunology 2022Quote: ... and the secondary antibody goat anti-mouse IgG (H+L) conjugated to Alexa Fluor 488 were obtained from Millipore Sigma. Monoclonal Rac1 antibodies (Cat ...
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bioRxiv - Biophysics 2022Quote: ... Terrific Broth (48.2 g per liter of TB supplemented with glycerol at 8 ml per liter) was augmented with Kanamycin (50 μg/mL) and antifoaming agent (approximately 15 drops in 1.5 L media; Sigma Aldrich). The media in 2 L bottles were kept at 37°C with bubbling for approx ...
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bioRxiv - Biophysics 2022Quote: ... the glass coverslips were coated with 50 μg ml-1 of poly-L-lysine (Sigma-Aldrich, St. Louis, MO, USA) for 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626), 0.4 mg/L biotin and 0.5% methanol ...
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bioRxiv - Developmental Biology 2022Quote: ... and then transfected into a suspension of Sf9 cells at 0.8×106 cells/mL grown in serum-free EX-CELL® 420 medium containing L-glutamine (Sigma) and incubated at 26°C with shaking for virus production ...
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bioRxiv - Cell Biology 2022Quote: ... human astrocytes are seeded (35,000/cm2) on the underside of the transwell insert precoated with Poly-L-lysine (2 µg/mL) (Sigma-Aldrich). Then ...
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bioRxiv - Cell Biology 2022Quote: ... 0.1-0.2×106 cells were seeded on sterile 16 mm borosilicate glass circle coverslips coated with poly-L-lysine solution (0.01%, sterile-filtered, P4832, Sigma-Aldrich) until 70-80% confluence ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 4 mM L-glutamine and 5% FBS and plated into poly-D-lysine-coated 96-well white polystyrene Nunc microplates (Sigma) at a density of 1×105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-CAMSAP2 was eluted in 50 μl TEV cleavage buffer containing 0.5 μg of glutathione S-transferase-6x-histidine Tobacco etch virus protease site (Sigma-Aldrich) for 2 hr at 4 °C.
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bioRxiv - Developmental Biology 2022Quote: ... replacing normal Lys and 0.09 mg/ml Arg-10 HCl L-Arginine HCl 13C,15N (Silantes; 201603902) replacing normal Arg or in media containing 0.15 mg/ml L-Lysine monohydrochloride (Sigma; L5626) as a Lys replacement and 0.09 mg/ml L-arginine monohydrochloride (Sigma ...
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bioRxiv - Immunology 2022Quote: The crown of the skull was aseptically removed and meninges were gently peeled from the underside and collected in 1.5 Eppendorf containing 400μL digest buffer (RPMI supplemented with 1mg/ml collagenase [Fisher scientific], 1mg/mL dispase [Sigma] and 40μg/mL DNAse [Sigma]). Meninges were digested in a 37 °C water bath for 30 minutes with intermittent shaking every 5-10 minutes ...
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bioRxiv - Genetics 2022Quote: ... were coated with 300uL per chamber of a 1:4 dilution of Poly-L-Ornithine Solution (0.01%, Sigma-Aldrich P4957) in Ultrapure water and incubated at 37C for 4 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... the precultures were inoculated to the initial OD600 of 0.05 in 15 mL minimal medium or minimal medium containing 2 g/L sodium malonate dibasic (Sigma-Aldrich) in 100 mL unbaffled shake-flasks ...
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bioRxiv - Synthetic Biology 2022Quote: ... cerevisiae strains for preparation of competent cells were cultivated in YPD medium consisting of 10 g/L yeast extract (Merck Millipore), 20 g/L peptone (Difco ...
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bioRxiv - Plant Biology 2022Quote: ... For the L-kyn split plate assay the following four treatment combinations were prepared: MM (modified Knop medium and 0.02% DMSO), KK (modified Knop medium, 10 µM L-kynurenine (Sigma-Aldrich) and 0.02% DMSO) ...
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bioRxiv - Microbiology 2022Quote: ... 200μL of either viral supernatant (VP4 mutants) or cell lysates (NSP3 mutants) diluted in 0.1M Carbonate-Bicarbonate Buffer (Sigma-Aldrich) was immobilized on clear 96-well ELISA plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2022Quote: ... TREx BCBL1-Rta cells expressing GFP-ORF11 were grown on sterilised glass coverslips treated with Poly-L-Lysine (Sigma-Aldrich). After 24 hours cells were washed in PBS and fixed in PBS containing 4% (v/v ...
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bioRxiv - Physiology 2022Quote: ... and 100 U/mL penicillin-streptomycin [Thermo Fisher Scientific Inc.]) and seeded on a glass-bottom dish (Iwaki Co. Ltd., Tokyo, Japan) with 0.01% poly-L-lysine (Millipore, Merck) coating ...
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bioRxiv - Bioengineering 2022Quote: ... 7 kDa:17 kDa) and Poly(D,L-lactide-glycolide) 50:50-b-PEG (10kDa PLGA, 2 kDa PEG) were purchased from Sigma.
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bioRxiv - Microbiology 2022Quote: ... Yeast were grown on 2% (w/v) agar plates containing yeast nitrogen base (YNB) without amino acids (6.8 g/l, Sigma-Aldrich) supplemented with yeast synthetic drop out (YSDO ...
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bioRxiv - Immunology 2022Quote: The excipients D-Sorbitol and L-glutamic acid monosodium salt monohydrate (MSG) were purchased from Sigma Aldrich (St. Louis, MO), and magnesium chloride hexahydrate (MgCl2.6H2O ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 13 mm cell culture coverslips coated with 0.01 mg/ml poly-L-lysine hydrobromide (Sigma-Aldrich) at a density of 50,000 cells/cm2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells transformed with the sRNA plasmids were cultured in 1.3 L of LB media (Roth, X968.4) supplemented with 1:100 ratio of 100 mg/mL ampicillin (Sigma-Aldrich, A9393) at 37° C until OD600 = 0.5 was reached ...
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bioRxiv - Physiology 2022Quote: 24-well tissue culture plates were coated overnight at room temperature with 0.5mg/mL Poly-L-lysine (Sigma Cat# P2636) in 0.5M borate buffer then washed twice with sterile PBS ...
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bioRxiv - Plant Biology 2022Quote: ... the water was removed and replaced with 100 μL of an aqueous solution containing 7.8 μg/mL L-012 (Sigma Aldrich) and 20 μg/mL horseradish peroxidase (Alfa Aesar ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A total of 1 × 105 cells (treated with vehicle or 6.4 µM THZ-P1-2 for 24h) were seeded per well in poly-L-lysine (Sigma-Aldrich) coated Seahorse XF96 plates in 200 μL XF Assay Medium (Modified DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... The surfaces of glass coverslips and plates were pre-coated with 1 μg/μl poly-L-lysine (Sigma-Aldrich, USA).
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bioRxiv - Neuroscience 2022Quote: ... Midbrain neurons were then seeded within a cloning cylinder on the #1.5 cover glasses pretreated with Poly-L-ornithine (Sigma, P3655). Cells were plated at a density of 199,000 cells / cm2 and grown in the Neurobasal-A based medium supplemented with GDNF (10 ng/mL ...