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Citations for Millipore Sigma :
7001 - 7050 of 8078 citations for L Asparagine H2O 13C4 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The collected cells were put on a microscope slide with a coverslip that had been coated in0.1% poly-l-lysine (Sigma-Aldrich). Cells were imaged using phase contrast microscopy and beads were imaged using epifluorescence microscopy with a filter for green fluorescent protein ...
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bioRxiv - Bioengineering 2019Quote: ... scaffolds were transferred to new wells containing 1.5 ml of basic media (BM) (CM supplemented with 200 μM L-Ascorbic acid 2-phosphate (Sigma-Aldrich)) ...
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bioRxiv - Synthetic Biology 2020Quote: ... All culture media were supplemented with either 20 g L−1 of D-glucose or maltose (both from Merck Millipore).
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bioRxiv - Cell Biology 2020Quote: ... One of the two dried metabolite pellets of each sample was re-suspended in 20 µl of the LC-MS-grade waters (Milli-Q 7000 equipped with an LC-Pak and a Millipak filter, Millipore). The re-suspended sample was mixed with 10 µl of 100 mM sodium carbonate (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... stochastic photoswitching of immunostained samples was initiated with a buffer containing 50 mmol L-1 2-mercaptoethylamine (M9768, Sigma-Aldrich), 40 µg mL-1 catalase (C3155 ...
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bioRxiv - Bioengineering 2019Quote: ... Solutions involving click chemistry also had an additional 25 mM of L-ascorbic acid and a 2.5 mM Cu2SO4 (Sigma Aldrich). Solutions were left over night to allow for conjugation ...
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bioRxiv - Bioengineering 2020Quote: ... stained with phalloidin/SYTOX green prepared as described previously were carefully placed on top of a 0.01% poly-L-lysine (Sigma Aldrich) coated 5 mm round coverslip ...
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bioRxiv - Pathology 2020Quote: ... a COX2 inhibitor that reduces inflammation was administered in the drinking water in a dose of 0.3 g/L of aspirin (Sigma, USA) from birth to 4 weeks(3).
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bioRxiv - Plant Biology 2021Quote: ... the pellets were resuspended with 100 μl of blocking solution (1X PBS, 1% BSA and 0.1% saponin) containing 50 μg/ml of TRITC-phalloidin (Sigma-Aldrich) and 2.5 μg/ml of 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Synthetic Biology 2020Quote: ... glass coverslips placed in 6-well plates were coated in a 0.1 mg/mL solution of poly-L-lysine hydrobromide (Sigma #P6282) for 5 min and left to dry overnight before plating 6×105 cells per well in 2 mL DMEM ...
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bioRxiv - Neuroscience 2019Quote: ... We plated the cells in 24-well plates on glass coverslips 13-mm in diameter coated using poly-L-lysine (0.01 mg/ml, Sigma-Aldrich) in Neurobasal medium (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... and lysed in 300 μl of lysis buffer (20mM Tris pH=8.0, 150mM NaCl, 1% Triton) containing 100 μM of N-Ethylmaleimide (Millipore-Sigma) and 1Xprotease inhibitor cocktail (Biotool ...
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bioRxiv - Pathology 2021Quote: ... the 5th injection of ketamine or SAL included a co-injection of L-DOPA (7 mg/kg, i.p., Sigma-Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in 167μL PBS with appropriate supplement and pipetted onto glass coverslips pre-treated with poly-L-lysine (Sigma; P4707) for 1 hr at 22 °C ...
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bioRxiv - Microbiology 2019Quote: ... Then 1 × 107 cells were washed with sterile water and transferred to nitrogen starvation conditions [6.7 g/L YNB without amino acids and without ammonium sulfate (Sigma-Aldrich) and 20 g/l glucose] and grown at 30 °C and 200 rpm ...
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bioRxiv - Microbiology 2021Quote: ... were added to a 5 L culture of BW25113(ΔFepA) cells over expressing FepA from a pBAD/Myc-HisB (Novagen) plasmid ...
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bioRxiv - Microbiology 2021Quote: ... Viruses were amplified on MDCK cells cultured in serum-free DMEM containing 0.5 μg/mL L-1-p-Tosylamino-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma–Aldrich). Stocks were titrated by plaque assays on MDCK cells.
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... or HMVEC-L were seeded at 4 × 104 cells per well onto EMD Millipore Millicell EZ Slides (PEZGS0416; Sigma Millipore), grown in complete medium for 48 hours ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... or HMVEC-L were seeded at 4 × 104 cells per well onto EMD Millipore Millicell EZ Slides (PEZGS0416; Sigma Millipore), grown in complete medium for 48 hours ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cells were lysed in 600 μl of SDS Lysis buffer (50 mM Tris-HCl pH 7.5, 1% SDS) containing a protease inhibitor cocktail (Sigma-Aldrich) and boiled at 90°C for 15 min ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... containing 10 μL droplets of KSOM (Zenith biotech) supplemented with 1 mg/mL BSA overlaid with embryo-suitable light mineral oil (Millipore) in 5% CO2 and 37°C ...
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bioRxiv - Pathology 2021Quote: ... rats in the high-dose 7-d L-DOPA priming group (n=6) were treated daily with L-DOPA (12 mg/kg) + benserazide (15 mg/kg, s.c., Sigma-Aldrich) for 7 d (i.e. ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were incubated with 10μM 1,2-dioleoyl-sn-glycero-3-phospho-L-serine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (18:1 NBD-PS) (1:300, Sigma-Aldrich) for 15 minutes at 25°C ...
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bioRxiv - Developmental Biology 2019Quote: ... anesthetized embryos at stage 35/36 (or stage 32 for EM) and cultured on 10µg/ml poly-L-lysine-(Sigma-Aldrich) and 10µg/ml laminin-(Sigma-Aldrich ...
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bioRxiv - Immunology 2019Quote: ... cultured in R10 for several days and activated with 5 μg/ml phytohaemagglutinin (PHA-L, Calbiochem, Millipore Sigma, Burlington, Massachusetts) overnight before addition of 500 IU/ml recombinant human IL-2 (R&D Systems).
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bioRxiv - Molecular Biology 2020Quote: ... or heat stress (42°C in L-15 Leibovitz medium in CO2-free environment) and co-treated with DMSO (0.1%, Sigma-Aldrich), MG132 (10 μM in DMSO ...
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bioRxiv - Physiology 2019Quote: ... the lead acetate/lead sulfide assay is setup by initially preparing the reaction mixture of 10mM L-cysteine (Sigma #168149) and 1mM pyridoxal phosphate (PLP ...
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bioRxiv - Physiology 2019Quote: ... all mice were treated with doxycycline in the drinking water around 10-14 weeks of age (1g/L; Sigma Aldrich) for 3 weeks followed by a wash out period of 3 weeks.
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bioRxiv - Physiology 2021Quote: Cells were grown in Dulbecco’s Minimal Eagles Medium (DMEM) with glutamine and 4.5 g/L glucose (Mediatech) supplemented with fetal bovine serum (10%; Sigma-Aldrich) and penicillin-streptomycin (0.1 U/L ...
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bioRxiv - Immunology 2020Quote: ... 2mM Glutamax; 10mM HEPES; 1x Antibiotic-Antimycotic; 1x N2 supplement; 1x B27 supplement; all ThermoFisher, and 1mM Acetyl-L-cycteine, Sigma) supplemented with EGF (50ng/ml ...
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bioRxiv - Cell Biology 2021Quote: 5 mL of yeast cells expressing the indicated constructs (see main text) were grown at 30°C in liquid YNB media (6.8 g/L) (Sigma-Aldrich) supplemented with 5 g/L glucose and 1.92 g/L synthetic drop-out medium without uracil (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... hPSCs were cultured in RPMI B27-medium (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Mesoderm was induced with RPMI B27- (RPMI 1640 GlutaMAX+ 2% B27 supplement without insulin, 200 μM L-ascorbic acid 2-phosphate sesquimagnesium salt hydrate (Sigma) and 1% Penicillin/Streptomycin (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded on coverslips coated with poly-L-ornithine (0.1mg/mL in borate buffer; PLO) (Sigma-Aldrich, MO, USA) or culture plates (Corning ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated for 16 hours with N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-phenylglycin T-butyl ester (DAPT) (Sigma Aldrich), an inhibitor of γ-secretase ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ND7/23 cells were plated on cover glass chips treated with 0.01% Poly-L-lysine (Sigma, St. Louis, MO, USA). Plated cells were transfected with plasmids encoding α- ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were plated on gold-nanorod-embedded coverslips (hestzig.com, part no. 600-400AuF) coated with poly-L-lysine (Sigma P4832) for 20 min prior to use ...
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bioRxiv - Cell Biology 2020Quote: ... and cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, 1 g/L glucose; Nacalai Tesque) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich). Apoptosis of the cells was initiated by adding anti-FAS antibody (125 ng/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of stationary phase cultures of the complemented strains were spotted in BHI agar plates supplemented with Congo Red (Sigma) 25 µg.mL-1 and IPTG 1 mM ...
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bioRxiv - Cell Biology 2021Quote: ... by digestion in 10mL/1g tissue of Enzyme Digest Solution (1mg/mL Dispase II, 0.5mg/mL Collagenase A and 1.5mg/mL DNAse l (Sigma-Aldrich, USA)) in advanced-DMEM/F12 (Thermofisher ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The diet was made with a fixed total amount of nutrients of 170 g/L (protein + carbohydrates) and 0.5% of vitamin mixture for insects (Sigma, Germany). Carbohydrates were supplied as sucrose (Euromedex ...
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bioRxiv - Cell Biology 2020Quote: ... Myoblasts were transfected with 20μl of concentrated lentivirus for 24h using growth medium supplemented with 4μg/ml polybrene transfection reagent (TR1003-G, Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... 10-15 days old astrocytic cultures were subject to a sublethal dose of H2O2 (0.7-1.5 mM) for 45 minutes and subsequently incubated in absence or presence of 500 uM L-carnitine (Sigma-Tau) for 2 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 20 mM taurine and 0.1% BSA) in Joklik-modified minimum essential medium (MEM) (one ampoule/l Joklik-MEM; M0518, Sigma-Aldrich) at a constant 6.4 ml/min flow of equilibrated 5% CO2/95% O2 ...
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bioRxiv - Cancer Biology 2020Quote: Primary lung fibroblasts were isolated from disaggregated tissue samples as described previously14 and cultured in DMEM supplemented with L-glutamine (1% v/v; Sigma), penicillin-streptomycin (1% v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... buffered to pH 8.3 with 1 M bicine) supplemented with 1 g/l of and Antibiotic antimycotic solution (1:100, Sigma, A5955). To dissociate into single cells ...
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bioRxiv - Cancer Biology 2021Quote: Primary glioblastoma cultures were grown in adherent culture on Corning Primaria dishes coated with poly-L-ornithine (Sigma-Aldrich, P4957) and laminin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and then VSMCs were treated in micro-volume by a pipette with CD (10 μmol/L; Sigma, St. Louis, MO), with ML7 (10 μmol/L ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tagged or direct knockout parasites lines from single T25 flasks were harvested and lysed in 50 μl lysis buffer (1% v/v Triton-X 100, 1 mM MgCl2, 1X cOmplete EDTA-free protease inhibitor cocktail (Sigma), 0.1 % v/v Benzonase (Merck) ...
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bioRxiv - Microbiology 2021Quote: ... was grown in BM2 supplemented with 20 μM MgSO4 (Fernandez et al., 2012) and 0.2% L-rhamnose monohydrate (Sigma-Aldrich) in the absence or presence of 0.5 μM TAT-RasGAP317-326 ...