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Citations for Millipore Sigma :
6651 - 6700 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Pelleted cells were then suspended in 3 ml of freezing medium per T75 culture flask (culture medium + 10% Dimethyl sulfoxide (DMSO, D5879, Sigma)) and progressively cooled in a cryocooler overnight at - 80° C ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were washed in 1x PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Microbiology 2024Quote: ... Indole-3-carboxaldehyde (I3A, catalog # 129445) and 6-Formylindolo[3,2-b]carbazole (FICZ, catalog # SML1489) were purchased from Millipore Sigma and reconstituted in DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma], 60 mM sucrose [S7903, Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... A portion of the LV and septum was minced in a beaker on ice for 3 minutes and subsequently was homogenized using a glass dounce homogenizer at 4°C for 3 minutes in 10 mL of isolation buffer (200 mM Mannitol [M9647, Sigma] ...
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bioRxiv - Cell Biology 2024Quote: ... Arteries were then stimulated with increasing concentrations of serotonin (5-HT, from 10–8 to 3·10–5 M, Sigma), endothelium-dependent vasodilator acetylcholine (ACh ...
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bioRxiv - Genetics 2024Quote: ... and 3-5 µl plasmid solution (Plasmid concentration 100-200 ng/µl) containing Phenol Red for visualization (0.1% Sigma #P0290) was injected intramuscularly using a Nanofil syringe (WPI ...
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bioRxiv - Genomics 2024Quote: ... followed by TBST washes (3 x 5 min) and then counterstaining with DAPI (20µg/ml in TBST, D9542, Sigma Aldrich). Slides were then mounted using an antifade reagent composed of 10% v/v Mowiol 4 to 88 ...
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bioRxiv - Biophysics 2024Quote: ... The PDMS scaffold surface was activated by oxygen plasma and then treated with a 5% (3-aminopropyl) triethoxysilane (Sigma-Aldrich) solution in methanol for 45 min ...
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bioRxiv - Pathology 2024Quote: ... 10 and 14 wounds were sectioned through the wound beyond the midpoint and wound centres identified by staining with Haematoxylin (Gills No.3) and Eosin (Sigma). All CWs were bisected.
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bioRxiv - Cell Biology 2024Quote: ... the coverslips were washed 3 times for 5 min with PBST and mounted on microscope slides using Mowiol (EMD Millipore) as the mounting agent ...
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bioRxiv - Immunology 2024Quote: ... The migrated neutrophils in the apical compartment were lysed with 0.5% Triton X-100 and neutrophil MPO activity was quantified using a colorimetric assay using citrate buffered 2,2’-azino-bis(3-ethylbenxothiazoline-6-sulfonic acid) diammonium salt (ABTS) solution (Sigma-Aldrich). After 10 min of incubation ...
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bioRxiv - Microbiology 2024Quote: ... mice were intranasally administered with 3 µg of the RSP protein and 5 μg of cholera toxin (CT; Sigma-Aldrich) as immunological adjuvant ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 minutes and washed 3-4 times in PBS before being mounted in Mowiol mounting media (Millipore, 475904-M).
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bioRxiv - Bioengineering 2024Quote: ... or 3-5×105 pelleted cells were lysed in TRI Reagent (“LS” for fluid EV samples, conventional for cells, Merck Millipore). Liquid chloroform was added to the lysates at a 1:5 v/v ratio and vigorously mixed for 30 s ...
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bioRxiv - Cell Biology 2024Quote: ... 3 days post-fertilization (dpf) larvae were anesthetized in E3 medium containing 0.2 mg/mL Tricaine (ethyl 3-aminobenzoate; Sigma-Aldrich) and maintained at 28.5 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... We washed the slides for 5 min in 2× SSC/0.1% IGEPAL at RT followed by a 3-minutes wash at 60 °C in 0.4× SSC/0.3% IGEPAL (Sigma-Aldrich Inc.), and an additional 3-minutes wash in 2× SSC/0.1% IGEPAL at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
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bioRxiv - Cell Biology 2024Quote: ... Moreover, a non-targeting control siRNA (NT siRNA, or siCON) with the sequence 5’-CGUACUGCUUGCGAUACGGUU-3’ was used (Sigma-Aldrich). Cells were transfected with 22 pmol of siRNA oligonucleotides/well using Lipofectamine RNAiMAX (ThermoFisher Scientifc ...
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bioRxiv - Neuroscience 2024Quote: ... Two length-matched siblings were anesthetized in 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich) simultaneously and mounted in 2% low-melting temperature agar ...
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bioRxiv - Immunology 2024Quote: ... approximately 30 g of spleen tissue was placed in R10 supplemented with 10U/mL DNase I and 3 mg/ml collagenase D (Sigma), the capsule was removed ...
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bioRxiv - Neuroscience 2024Quote: ... The preparation was bathed in standard locust saline (in mM: 147 NaCl, 10 KCl, 4 CaCl2, 3 NaOH, 10 HEPES buffer; pH adjusted to 7.2; chemicals from Sigma-Aldrich) and grounded with a chlorided silver wire in the abdomen ...
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bioRxiv - Bioengineering 2024Quote: ... silicone plates were plasma treated for 5 minutes followed by incubation with 10% (3-aminopropyl)triethoxysilane (APTES; #440140; Sigma-Aldrich) in ethanol at 60°C for 60 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in 3%milk for 1hr at room temperature and then incubated with the primary anti-a-tubulin antibody T6199 (Sigma) 1:100 000 in I-Block overnight at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... IPs were then washed 4x and eluted 3x at 30°C with 0.5 mg/ml of 3×FLAG peptide (Sigma, F4799) buffered in 1×PBS plus 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... The reaction mix was incubated at 37°C for 20 min and then filtered using a 3-kDa cutoff filter (Millipore) at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM EDTA) with 0.5 mg/mL lysozyme and 3 mg/mL proteinase K (Sigma-Aldrich Corp., St. Louis, MO). DNA isolation was performed with PureLink Genomic DNA Mini Kit (Thermo Fisher Corp. ...
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bioRxiv - Microbiology 2024Quote: ... embryos were mechanically dechorionated if necessary and anaesthetised in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The zebrafish embryos were individually infected by microinjection with 1 nl of S ...
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bioRxiv - Neuroscience 2024Quote: ... The rest of the sample was divided into three tubes and incubated overnight rotating at 4 °C with 3 μl of mouse anti-VE-cadherin antibody (MABT134, Millipore) or donkey anti-mouse IgG (A16013 ...
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bioRxiv - Immunology 2024Quote: VSV-G pseudotyped HIVNL4-3 XCS and HIVNL4-3ΔNef were produced by transfection of 293T cells using CAPHOS kit (Sigma-Aldrich) as in 63 ...
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bioRxiv - Biophysics 2024Quote: ... labelled BGZP-C were prepared in 25 mM 2H-glycine buffer at pH 11.0 or 25 mM phosphate buffer at pH 6.0 in either straight 3 mm tubes (Wilmad, Vineland, NJ) or 5 mm susceptibility-matched microcells (Shigemi, Sigma-Aldrich). Separate samples ...
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bioRxiv - Immunology 2024Quote: ... bEnd.3 or ImmCPE cell lines were grown on transwell collagen-coated membrane 0.4um pore size inserts (CLS3495-24EA; Sigma-Aldrich) in a 24-well plate ...
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bioRxiv - Cancer Biology 2024Quote: ... each coverslip was washed with PBS 3 times each for 5 minutes and nuclei were stained with Hoechst (Merck Sigma, Burlington ...
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bioRxiv - Neuroscience 2024Quote: Larvae were anesthetized in 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) prior to imaging ...
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bioRxiv - Neuroscience 2024Quote: ... Retinas were then washed in 1x PBS for 3×30 minutes at RT and mounted using Fluoromount aqueous mounting medium (Sigma).
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bioRxiv - Neuroscience 2024Quote: ... in primary neuronal lysate after Aβ or gAβ treatment from mouse or human brain were quantified by the Glycerol-3-Phosphate Assay Kit (Sigma), High Sensitivity DHAP Fluorometric Assay Kit (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... Total RNA was extracted from the central body cells of 3-week-old WT and Col5a1+/− menisci by homogenizing freshly dissected tissues in TRI-reagent (T9424, Sigma) and phase-separated in 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were then washed x3 in 0.24M phosphate buffer solution (PB)(Table 3) and left overnight in 0.12PB with 15% Sucrose (Sigma, 84100-1KG) at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then collected by centrifugation and washed with 3% FBS in PBS followed by PI (Millipore Sigma, catalog P4170) staining (25 μg/mL PI ...
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bioRxiv - Biochemistry 2024Quote: ... the column was washed using 50 mL of 10 mM Tris buffer (pH 7.4) containing 100 mM NaCl and 3 % Empigen BB (Sigma-Aldrich) to replace urea and SDS with Empigen ...
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bioRxiv - Biochemistry 2024Quote: ... the enzyme was incubated with 100 μM KCN or 500 μM 3-amino-1,2,4-triazole (ATZ, Sigma, Cat# A-8056) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... 10 min) and resuspended in 3-5 ml lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5xCellLytic B [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2024Quote: ... bacterial cultures in the exponential phase were collected and then thoroughly mixed with 3 wt% (w/v) of sodium alginate (Sigma) solution to a final concentration of 2.5% alginate ...
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bioRxiv - Immunology 2024Quote: ... Non-specific binding sites were blocked using 300 µl/well of 3% (w/v) skim milk powder (Sigma-Aldrich, USA) in PBS-T ...
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bioRxiv - Developmental Biology 2024Quote: Cardiac cells were fixed with cold 4% paraformaldehyde (PFA) solution for 20 min at RT and then washed three times in PBS and once in 3% BSA (Sigma) in PBS for 5 min ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were cultivated under humidified conditions with 5% CO2 at 37°C and passaged every 3-4 days using 0.05% trypsin/EDTA (Sigma-Aldrich). Cells were harvested in ice-cold PBS buffer using a cell scraper ...
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bioRxiv - Immunology 2024Quote: ... infected cells at different time-points were washed 3 times in 1xPBS and lysed with 0.1% Triton X-100 (#X100 Sigma-Aldrich) in H2O ...
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bioRxiv - Immunology 2024Quote: ... cells were stimulated for 3 hours with 50 ng/ml phorbol myristic acid (PMA; Sigma-Aldrich, St Louis, MO, USA), 1 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... followed by activation with 50 mM 3-Dimethylamino-propyl)-ethyl-carbodiimide Hydrochloride (EDC)/100 mM N-Hydroxysuccinimide (NHS) (Sigma-Aldrich) for 12 min at 10 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then washed 3 times in 0.01% PBS-Tx (1xPBS with 0.01% Triton X-100 (Sigma-Aldrich, Cat#X100)) for 10 mins at RT ...