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Citations for Millipore Sigma :
6701 - 6750 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were suspended and incubated for 3 min in 350 µl PBS mixed with 150 µl 0.4% trypan blue solution (Millipore Sigma). For determining viability ...
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bioRxiv - Plant Biology 2024Quote: All dried phosphopeptide samples were dissolved in a 3% ACN (v/v) / 0.1% (v/v) TFA solution and desalted using ZipTip C18 pipette tips (Millipore; ZTC18S960) as previously described (29) ...
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bioRxiv - Plant Biology 2024Quote: ... The stationary phase was comprised of a Discovery HS F5 (150 x 2.1 mm, 3 μm particle size, Sigma-Aldrich) pentafluorophenyl (PFP ...
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bioRxiv - Pathology 2024Quote: ... the AI algorithm was validated against a standard low-resolution image-based caspase-3/7 assay in a proof-of-concept toxicity study where BBB organoids were treated with staurosporine (S5921, Sigma) to induce apoptosis27 ...
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bioRxiv - Developmental Biology 2024Quote: ... These eggs were then fertilized and injected in the presence of 1mM 3-amino triazole (Sigma, St. Louis, MO, USA) to prevent crosslinking of fertilization envelopes ...
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bioRxiv - Physiology 2024Quote: ... They were then challenged for 3 hours on tubes containing ND supplemented with 0.5% Erioglaucine disodium salt (Sigma Aldrich, #861146). Flies were frozen as group of 10/1.5ml Eppendorf tube until OD measurements at 629nm as described previously 59.
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bioRxiv - Neuroscience 2024Quote: ... Anatomical images were acquired from fish anesthetized with 0.2 mg/ml ethyl-3-aminobenzoic acid ethyl ester (MESAB, catalog # E10521, Sigma-Aldrich). To activate TRPV1-expressing Purkinje cells ...
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bioRxiv - Neuroscience 2024Quote: Adult mosaic zebrafish (F0) were anesthetized in pH-buffered ethyl 3-aminobenzoate methanesulfonate solution (0.2 mg/ml; MS-222, Sigma-Aldrich). A section of the caudal fin was removed using dissecting scissors (WPI Inc. ...
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bioRxiv - Neuroscience 2024Quote: ... Growing cells were trypsinized and seeded at 3 × 103 cells per 9.5-mm multi-well glass bottom dish (D141400, Matsunami) coated with fibronectin (F0895, Sigma-Aldrich). Aliquots (1 μL ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was stirred under N2 for 5 minutes before 0.71 mg (1.05 eq) of 3-morpholinopropylamine (Sigma-Aldrich Inc) was added ...
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bioRxiv - Neuroscience 2024Quote: ... cells were harvested in PBS with protease inhibitors (1mM PMSF, Phosphatase Inhibitor Cocktail 3 [Sigma-Aldrich, P004] and Complete [Roche]) and centrifuged for 3 min at 500g at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... overnight at 4°C and then incubated for 3 hrs to overnight in 5% non-fat milk or 5% bovine serum albumin (Sigma) in TBS-T with primary antibodies against MPC1 (Cell Signaling ...
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bioRxiv - Biophysics 2024Quote: ... 100 µL of a 10 mg/mL solution of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipids (Sigma-Aldrich, P6354) and 0.05–0.2 mol% biotinylated lipids (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(biotinyl ...
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bioRxiv - Biophysics 2024Quote: The microfluidic chips were incubated with ∼3 mg of photoactivatable silane polyethylene glycol (PEG) (68) in 1.5 mL of anhydrous acetonitrile (Sigma-Aldrich) for ∼30 min ...
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bioRxiv - Biophysics 2024Quote: ... fluorescent labelled tRNAGln and tRNATrp were isolated instead using 5’ - CAA AAA CCG GTG CCT TAC CGC TTG GCG - 3’/3AmMO/ and 5’ - TTT GGA GTC GAA AGC TCT ACC ATT - 3’/3AmMO/ DNA oligos, respectively, with 3’ amino-modifications (3AmMO, IDT) conjugated to N-Hydroxysuccinimidyl (NHS)-Sepharose 4 Fast Flow beads (H8280, SIGMA).
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bioRxiv - Bioengineering 2024Quote: ... 5 µL of cell suspension was mixed with 5 µL of fibrinogen solution (6 mg/mL, final concentration 3 mg/mL, 8630, Sigma) by pipetting up and down three times ...
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bioRxiv - Biophysics 2024Quote: ... Collected RNA eluents were concentrated and buffer exchanged into sterilized nanopure water using 3-kDa or 10-kDa MWCO Amicon devices (Millipore), supplied with counterion in high salt buffer (10 mM sodium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... the resulting protein was concentrated to a final volume of 500 μL using an Amicon Ultra-15 Centrifugal Filter Unit with a 3 kDa molecular weight cut-off (Millipore). The protein was further purified through SEC using a Superdex™ 75 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Biophysics 2024Quote: ... The Ni-NTA eluate was supplemented with 3 mM CaCl2 and applied to M1 anti-FLAG immunoaffinity resin (Sigma-Aldrich) pre-equilibrated with FLAG wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then decorionated with 1mg/mL Pronase and anaesthetised immediately prior to microinjection using 0.2mg/ml Tricaine (Ethyl 3-aminobenzoate methanesulfonate) solution (Sigma-Aldrich). The injection solution (containing either cells as previously described ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times for 5 min in PBS before blocking for 1h at room temperature with 3 % BSA in PBS-Triton 0.01 % (Triton X-100, Sigma, T8787). Cells were then incubated with primary antibody in blocking buffer (3 % BSA in PBS-Triton 0.01 % ...
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bioRxiv - Cell Biology 2024Quote: A 400 mM Auxin stock in ethanol was prepared from natural auxin indole-3-acetic acid (IAA, Sigma Cat #I3750) as described in [51] ...
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bioRxiv - Biochemistry 2024Quote: ... thawed GST-BirA enzyme (20 µL of 50 µM solution) and D-Biotin (3 µL of 50 mM solution in 100 % DMSO; Sigma) and incubated for 1 h minimum at 30 °C in an incubator with gentle shaking (90 rpm) ...
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bioRxiv - Biochemistry 2024Quote: RNA oligonucleotides derived from MALAT1 (5’-AACUU AAUGU UUUUG CAUUG GACUU UGAGUU -3’) were synthesized with or without m6A modification (Sigma). Oligonucleotides and the indicated pri-miRs were radiolabeled at 5’ ends with γ32P-ATP using T4 polynucleotide kinase ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with 3 mL of PBS and incubated with 20 mM Ammonium Hydroxide (Sigma, cat# 221228-100ML-A). The dishes were constantly shaken for 5 minutes at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Sftpc-CreERT2 and Rosa26R-CAG-lsl-tdTomato (R26R-tdTomato) mice were given 3-4 doses of tamoxifen (100 mg/kg, i.p.; Sigma-Aldrich).
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bioRxiv - Cancer Biology 2024Quote: To understand its role in ferroptosis we treated Control Sp and mitomiR-3 Sp cells with 0.5μM RSL3 (GPX4 mediated ferroptosis inducer) (Sigma-Aldrich, USA) and 10μM ferrostatin-1 (ferroptosis inhibitor ...
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bioRxiv - Biochemistry 2024Quote: ... the immobilized protein was incubated for 30 min with MIND-elution buffer (Table 3) containing 200 µg/mL 3x FLAG peptide (F4799; Sigma). Eluate from the anti-FLAG resin was collected and further purified by size exclusion chromatography in MIND-SEC buffer on a 120 mL Superdex 200 column (28-9893-35 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was collected and concentrated approximately 3-fold using a 10 kDa cut-off Amicon Ultra-15 centrifugal filter unit concentrator (Millipore). Approximately 2.0 mL of concentrated protein was subjected to size-exclusion chromatography using the Äkta Pure system and a Superdex 200 increase 10/300 gl column pre- equilibrated with running buffer (100 mM Tris-Cl pH 7.5 ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mL was added dropwise to the walls of a 15 mL falcon tube containing 3 mL (one and a half blood volume) Histopaque-1077 (Sigma) held at 60° to the ground ...
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bioRxiv - Bioengineering 2024Quote: ... N-hydroxysuccinimide (NHS) and N-(3-(dimethylamino)-propyl)-N-ethylcarbodiimide hydrochloride (EDC) were bought from Sigma-Aldrich (St. Louis, US), Roswell Park Memorial Institute (RPMI 1640 ...
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bioRxiv - Cancer Biology 2024Quote: ... Waters) equipped with a C18 reverse phase column (2.1 × 100 mm, 3 μm, Atlantis) with HPLC-grade solvents (Sigma Aldrich). High-resolution mass spectra (HRMS ...
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bioRxiv - Cell Biology 2024Quote: ... beads were washed on a magnetic stand 3 times with 80% ethanol and then resuspended in 50mM TEAB (Sigma-Aldrich). 1µg trypsin (PierceTM ...
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bioRxiv - Cell Biology 2024Quote: ... the membrane was immunoblotted for 16 hours at 4 °C in blotting buffer (3% BSA in TBS with 0.1 % Tween-20 (Sigma, P7949) with 1 μg/ml rabbit anti-MTNR1B (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... worms were washed in RPMI and anaesthetised via a three-minute incubation in 0.25 % tricaine (ethyl 3-aminobenzoate methanesulfonate, #A5040, Sigma-Aldrich). From this point ...
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bioRxiv - Developmental Biology 2024Quote: ... The cell pellet was gently resuspended in 100 µL lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2 (all Sigma Aldrich), 0.05% Tween-20 (Carl Roth) ...
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bioRxiv - Developmental Biology 2024Quote: ... samples were blocked and permeabilized with blocking buffer (PBS supplemented with 3% normal donkey serum [Sigma, S30] and 0.3% Triton X-100 [Sigma, T8787]) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Neuroscience 2023Quote: ... Nunc glass bottom dishes with an inner diameter of 27 mm were coated with Poly-L-Ornithine (Sigma-Aldrich P4957, 0.01% stock diluted 1:3 in PBS) overnight at 37° C ...
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bioRxiv - Neuroscience 2024Quote: ... Nunc glass bottom dishes with an inner diameter of 27 mm were coated with Poly-L-Ornithine (Sigma-Aldrich P4957, 0.01% stock diluted 1:3 in PBS) overnight at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... pH 7.3∼7.4) and then vacuumed infiltrated using a vacuum drying cabinet (Yiheng, Shanghai, China) with a vacuum pump (Millipore, MA, USA) for 30 min ...
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bioRxiv - Plant Biology 2020Quote: ... 2015) e cleaned using stage-tips preparations with 3 plugs of Empore polystyrenedivinylbenzene (SBD-XC) copolymer disks (Sigma Aldrich, MO, USA) for solid phase extraction following the manufacturers instructions.
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bioRxiv - Synthetic Biology 2021Quote: ... Extracts were then clarified at 4’000 × g for 10 min and concentrated to ~4 mg/mL total protein concentration using 3 kDa molecular weight cutoff (MWCO) Centriprep concentrators (EMD Millipore) to account for dilution during preparation ...
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bioRxiv - Biochemistry 2020Quote: Gene expression of endogenous CENP-C was inhibited using a single siRNA (target sequence: 5’-GGAUCAUCUCAGAAUAGAA-3’ obtained from Sigma-Aldrich), which targets the coding region of CENP-C mRNA ...
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bioRxiv - Cell Biology 2020Quote: ... cells were single-cell sorted into 96-well low-bind PCR-plates [Eppendorf] containing 3 μl of lysis buffer (0.5 units/μl RNase inhibitor [Takara], 0.15% Triton X-100 [Sigma], 0.5 mM (each) dNTP [Thermo Scientific] ...
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bioRxiv - Cell Biology 2020Quote: NHP heart slices seeded with HVPs/CMs after RFA injury were loaded with 3 μM Fluo-4-AM in CCM (without phenol red) supplemented with 0.75% Kolliphor EL (Sigma Aldrich, USA) by incubation at 37°C for 60 minutes ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies at appropriate dilutions (Supplementary Table 3) were prepared in blocking buffer with 0.1% Tween 20 (Sigma, 9005-64-5). The membrane was incubated in the primary antibody solution overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... was generated by cloning the 3HA epitope CDS fused in-frame with the 3′ end of the RNAi resistant dCNBP CDS into the UAS-attB vector (Genewiz, SIGMA-ALDRICH). The plasmids for inducible expression of the human CNBP counterpart was generated by cloning the FLAG epitope CDS fused in-frame with the 3′ end of the hCNBP CDS (CNBP-201 splice variant ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then incubated in LB for 3 hours at 37 °C and plated in LB agar with Kanamycin (Sigma-Aldrich) for 24 hours at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg of each channel was combined for buffer exchange on a 3 kDa molecular weight cut-off column (EMD Millipore) into 100 mM ammonium bicarbonate and digested overnight at 37 °C with mass spectrometry grade trypsin gold (Promega ...