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Citations for Millipore Sigma :
6851 - 6900 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified in 40 μL reactions with 15-18 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and 500 nM of each primer P1.3 and P2.1 (synthesized by IDT ...
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bioRxiv - Molecular Biology 2020Quote: ... 4OHT treated cells were washed 3 times with pre-warmed PBS and further incubated with 500 µg/mL auxin (Sigma; I5148). For transcriptional inhibition ...
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bioRxiv - Neuroscience 2020Quote: ... few selected free floating sections corresponding to the Somatosensory cortex were mounted on glass using chromium (3) potassium sulfate dodecahydrate (Sigma-Aldrich) and left to dry overnight ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from iCD4-PRMT5fl/fl and iCD4-PRMT5Δ/Δ mice (n = 3 pooled mice/sample and n = 3 samples/group) were lysed in our in-house lysis buffer (50 mM triethylammonium bicarbonate, Millipore Sigma, catalog no ...
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bioRxiv - Immunology 2020Quote: ... To activate the NLRP3 inflammasome BMMs were first primed with LPS in cell culture medium or left unstimulated for 3 h before cells were treated with 5 µM nigericin (Sigma-Aldrich) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with TBS-T (3 times for 10 min) and incubated with horseradish peroxidase-conjugated secondary antibodies anti-mouse (Millipore, #AP308P), or anti-rabbit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The uncoupled maximal capacity of the electron transport system (ETS) was obtained after titration with 0.5 μM of uncoupler carbonyl cyanide 3-chlorophenylhydrazone (CCCP, Sigma Aldrich, C2759) up to the complete dissipation of the proton gradient ...
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bioRxiv - Neuroscience 2021Quote: ... the reporter cells were exposed to drugs or DMSO vehicle for one hour before the addition of 3 ng/mL IL-1a (Sigma, I3901), 400 ng/mL C1q (MyBioSource ...
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bioRxiv - Microbiology 2021Quote: Calu-3 barrier integrity was determined by trans-epithelial electrical resistance (TEER) measured with a Millicell-ERS2 Volt-Ohm meter (Millipore Sigma). Barrier integrity was further assessed using the Human E-cadherin Quantikine enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Biophysics 2021Quote: ... the cells were washed 3 times with 500 µl of PBS and then were fixed and permeabilized with ice-cold 100% methanol (Sigma Aldrich) for 15 minutes at -20°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and the pellet resuspended and incubated for 3-7 minutes at 37°C in accutase dissociation reagent (Sigma, Poole, UK, A6964). After enzyme neutralization with fresh medium ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...
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bioRxiv - Microbiology 2022Quote: ... Tissue sections were then permeabilized overnight at 4°C by gently rocking in blocking buffer comprising 3% bovine serum albumin (Sigma Aldrich), 1% saponin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... 10 µl of hemolymph was mixed with 90 µl of 3, 4-Dihydroxy-L-phenylalanine (L-DOPA, 4 mg/ml)(Sigma Aldrich) dissolved in nuclease free water (Cytiva) ...
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bioRxiv - Genetics 2022Quote: ... The external ammonium concentration was estimated as a function of time for 3 hours using the below-mentioned principle (Ammonia Assay Kit - Sigma-Aldrich). At each time point ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was spun at 1000g for 3 minutes and re-suspended in islet media-G11 mixed with concentrated lentivirus and DEAE-Dextran hydrochloride (Sigma Millipore). The mixture was loaded to the GravityPLUS hanging drop 96-well microplate (InSphero ...
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bioRxiv - Pathology 2022Quote: ... at room temperature for 10 minutes and washed 3 times in PBS containing 0.1 % v/v Triton X-100 (Sigma-Aldrich X100), blocked for 30 min using 10 % v/v chicken serum (Gibco 16110082 ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Bioengineering 2022Quote: ... the 11-mercaptoundecanoic acid (CAS no: 71310-21-9) and L-Tryptophan (CAS no: 73-22-3) were obtained from Sigma Aldrich. The RBD ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed two more times with PBS (Biotech, PD8117) then blocked and permeabilised using a 3% BSA (Sigma-Aldrich, 1073508600), 0.2% Triton X-100 (VWR ...
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bioRxiv - Synthetic Biology 2020Quote: ... magnesium sulfate (MgSO4), calcium chloride (CaCl2), 3-(N-morpholino)propane sulfonic acid (MOPS), and hydrogen peroxide (H2O2, 30%) were from Sigma Aldrich. N-3-oxo-dodecanoyl-L-Homoserine lactone (AHL ...
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bioRxiv - Cancer Biology 2020Quote: ... and 50,000 live cells were spun down and incubated 3 min at 4oC in 25µl of a detergent buffer containing 0.2% Igepal CA-630 (Sigma-Aldrich, St.Louis, MA), 0.2% Tween 20 (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) and Griess reagent were purchased from Sigma-Aldrich (St. Louis, MO, USA). Penicillin ...
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bioRxiv - Bioengineering 2020Quote: ... Chromatographic separation (further desalting) was performed on a Discovery® BIO Wide Pore C5 column (100 × 2.1 mm, particle size 3 μm, Sigma-Aldrich) at 55 °C with a mobile-phase system consisting of water and acetonitrile (each containing 0.1% formic acid) ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Microbiology 2020Quote: ... and after 3-5 days transplanted into pots containing autoclaved vermiculite and sterile 0.5 × Murashige and Skoog (MS) medium (Sigma Aldrich, USA) with no carbon supplement ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were dispersed with Accutase at d45 of differentiation and plated at a concentration of 3×105 cells/well into MEA plates in 50 μL of culture media containing 10 μg/mL laminin (Sigma, 11243217001). One hour after plating ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in 3 ml StemFlex containing 10 μM Thiazovivin (Millipore, #S1459). The cells were subjected to a 48 hour cold shock at 32 °C to enhance homology directed repair (HDR) ...
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bioRxiv - Molecular Biology 2021Quote: ... the coating solution was discarded and the plate was washed 3 times with 300 µl of wash buffer (1xTBS, 1mM CaCl2, 2mM MgCl2, 0.25% Triton X-100 (Sigma-Aldrich, T8787)) ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was sterile filtered through a 0.22 μm 0.5 or 1L vacuum filter and concentrated using the Amicon 200 mL Stirred Cell (UFSC20001) with a 3 kDa NMW Ultrafiltration Disc (Millipore PLBC06210) and Amicon Ultra 15 mL Centrifugal Filters with a 3kDa NMW (Millipore UFC9003) ...
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bioRxiv - Biochemistry 2020Quote: ... The panel of substances tested included several nucleosides/nucleotides or derivates (ATP, ADP, 5’-AMP, 3’-AMP, cyclic AMP, adenosine, GTP, TTP, inosine, sodium polyphosphate, Sigma-Aldrich), biogenic amines (epinephrine ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Immunology 2020Quote: Urine cells were thawed into 9 ml of 10% FCS-RPMI medium supplemented with 10 IU/ml Benzonase (Novagen 70664-3), counted and viability determined by trypan blue exclusion ...
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bioRxiv - Immunology 2021Quote: Detection of IgA spots was achieved by using anti-IgA-conjugated horseradish peroxidase and developed with 3-amino-9ethylcarbazole (Sigma-Aldrich). Color development was stopped by washing several times with water ...
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bioRxiv - Immunology 2020Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1 μm centrifugal filter (Millipore ...
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bioRxiv - Immunology 2020Quote: ... The IHC protocol mirrors the MIBI-TOF protocol with the addition of blocking endogenous peroxidase activity with 3% H2O2 (Sigma Aldrich) in ddH2O after epitope retrieval ...
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bioRxiv - Cancer Biology 2020Quote: ... the thiol groups of the above extracted protein samples from the culture supernatant of 3 groups were alkylated with 50 mM iodoacetamide (cat No. I6125, Sigma, USA) (30 min in dark at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... The depleted serum was concentrated and the buffer was exchanged to 100 mM TEAB using Amicon 3 K concentrators (cat No. UFC500396, Millipore, USA). The procedures for serum protein denaturation ...
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bioRxiv - Genomics 2021Quote: ... CAD and Neuro-2a cells were grown on Millicell Hanging Cell Culture Inserts with a pore size of 3 μm (Millipore #MCSP06H48), adapting experimental pipelines described earlier (Ludwik et al. ...
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bioRxiv - Genetics 2020Quote: ... Samples were immunoprecipitated with S9.6 antibodies (8 μ antibody/ 5μ DNA) overnight at 4°C followed by incubation for 3 hours with protein G-Sepharose beads (Millipore) and washed sequentially ...
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bioRxiv - Cell Biology 2021Quote: β-Estradiol induction of the pRPS5A::XVE:GFP-μHD line was done by transferring 3-day-old seedlings to medium containing β-Estradiol (Sigma-Aldrich) or solvent (DMSO ...
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bioRxiv - Physiology 2021Quote: ... Samples were then immersed in 5% (v/v) H2O2 in methanol for up to 3 hours (Sigma-Aldrich Cat No: H1009) for bleaching ...
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bioRxiv - Neuroscience 2020Quote: ... 3-5 kDa fluorescein isothiocyanate-dextran (FITC-dextran) and 65-85 kDa tetramethylrhodamine isothiocyanate (TRITC-dextran) were obtained from Sigma-Aldrich. Transwell permeable polyester membranes (400 nm pore ...
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bioRxiv - Neuroscience 2021Quote: SH-SY5Y and HEK293 cells were treated with SB202190 (20 μM) for 3 h in the presence or absence of 100 nM Mito-TEMPO (Sigma-Aldrich) for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... Depletion of endogenous Spindly was achieved through reverse transfection with 50 nM Spindly siRNA (5′-GAAAGGGUCUCAAACUGAA-3′ obtained from Sigma-Aldrich) for 48 hours with RNAiMAX (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were combined and concentrated approximately 3-fold using a 30-kDa cut-off Amicon centrifugal filter unit (Millipore, Massachusetts). Approximately 5mL of concentrated protein was subjected to size-exclusion chromatography using the Äkta Pure system and a superdex 200 increase 10/300 gl pre-equilibrated with running buffer (50mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then eluted from the beads with 100 μL of lysis buffer containing 100 μg/mL 3 × FLAG peptide (F4799, Sigma Aldrich), mixed with sample buffer and incubated on ice for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... bacteria were isolated by centrifugation (12,300 x g, 3 minutes) and resuspended in 200 µL phosphate-buffered saline (PBS; Sigma-Aldrich, USA). Resuspended cells (200 µL ...