Labshake search
Citations for Millipore Sigma :
6551 - 6600 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Cultures were induced by addition of DNA-damaging antibiotics to a final concentration of 3 μg/ml: mitomycin C (Sigma), norfloxacin (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... pneumoniae serotype 3 (ST3) with or without addition of either Hyaluronidase from bovine testes Type I-S (H3506 Sigma-Aldrich, St ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were incubated in NBT (nitro-tetrazolium blue)-BCIP (5 bromo-4-chloro-3’-indolyphosphate p-toluidine) (Sigma-Aldrich), 0.033% in APTMg in dark condition ...
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bioRxiv - Biophysics 2024Quote: ... Fluorecent probe Atto488-DOPE (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto-488) was purchased from Sigma-Aldrich (international). Sucrose ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Plant Biology 2024Quote: ... a 188 bp cDNA fragment of MtABCG40 5’UTR or a 200 bp cDNA fragment of MtLOG3 3’UTR were amplified with KOD Hot Start DNA Polymerase (Novagen) and cloned into pDONR/Zeo vector (Thermo Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... All antibody incubations were done in block solution containing 5-10% Normal Donkey serum and 3% Bovine Serum Albumin (BSA, Sigma). After early initial staining ...
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bioRxiv - Genomics 2023Quote: ... with 10% charcoal-stripped FBS (#100-119, GemBio) for 3 days and then were stimulated with 10nM dihydrotestosterone (DHT) (A8380, Sigma) for 0.5 ...
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bioRxiv - Immunology 2024Quote: ... 20 μg cell proteins or 3-5 μg EVs proteins were denatured in LDS sample buffer containing 1M Dithiothreitol (DTT, Sigma) for 10 min at 70°C and loaded onto a 4-12% precast polyacrylamide gels for electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: Samples were incubated with 20 µL of IS solution (155 µmol/L forchlorfenuron, 25.5 µmol/L 4-chlorphenylacetic acid, 5.16 mmol/L indole-3-acetic-2,2-d2 acid, Sigma-Aldrich, Steinheim, Germany) and diluted with an acetonitrile/methanol + 1% formic acid solution (4:1 ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with U6-gDNA (5’-AAAGACGTCCCTAACAAGT-3’; clone ID es: HSPD0000063884): CMV-eCas9-2a-tGFP (Sigma-Aldrich). 48h after transfection ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Biophysics 2023Quote: ... The purified Nup98 was concentrated to a final concentration of 165 µM using 3-kDa MWCO centrifugal filters (Merck Millipore) in a solution of 2 M GdmCl ...
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bioRxiv - Cell Biology 2023Quote: ... P1 virus was used to infect 50 ml of SF9 culture and P2 virus was harvested after 3-5 days through centrifugation and subsequent filtration through 0.45 μm PVDF membrane (Merck Millipore, SLHV033RS). P2 virus was either propagated further or stored at 4ºC in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then washed two times with PBS and incubated for 45 min with the corresponding secondary antibody (Table 3) diluted in blocking buffer plus DAPI (Sigma) in darkness ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL of each sample was injected onto a ZIC-pHILIC 2.1 3 150 mm (5 mm particle size) column (EMD Millipore). Buffer A was 20 mM ammonium carbonate ...
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bioRxiv - Immunology 2022Quote: ... Samples were permeabilized in 0.1% Triton-X-100 for 3 min and then incubated in a blocking solution consisting of 10% FBS (Sigma-Aldrich), 0.01% sodium azide (Sigma-Aldrich) ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... To the bead suspension was added 0.5 mL of 750 mM of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in water (Sigma-Aldrich) and the mixture was incubated for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: Yp strains were induced to upregulate expression of the T3SS and associated effectors by taking 100 μL of stationary phase overnight cultures and adding to 3 mL 2x YT media containing 20mM sodium oxalate (Sigma) and 20mM MgCl (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed in TBS (3 washes for 5 min each) and then treated with 0.25% H2O2 (Cat#216763, Sigma-Aldrich), in TBS for 3 min ...
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bioRxiv - Microbiology 2023Quote: The small interfering RNAs (siRNAs) against NLRP3 (siNLRP3) (5’-UGCAAGAUCUCUCAGCAAA-3’) and the corresponding control siRNAs (siControl) (5’-UUCAAUAAAUUCUUGAGGU-5’) were synthesized from Sigma. The siGenome smart pool siRNAs and siON Target plus siRNAs were purchased from Dharmacon and are composed of a pool of four siRNAs ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 32P-γ-ATP was removed by washing with 3 column volumes of Millipore water and centrifugation in 10 kDa (Qβ-RNA) or 3 kDa (8mer) Amicon filters (Merck Millipore) at 14,000 rpm at 4 °C for four times ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were rinsed again 3 x 5min with ddH2O at room temperature and incubated in thiocarbohydrazide (TCH, 0.22mm Millipore filtered) solution for 20 min at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... The wells were washed with ddH2O for 3 times and incubated with 100ul/well of 10μg/ml fibronectin bovine plasma (Sigma-Aldrich) in 0.1% gelatin for 2 hours on an orbital shaker at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Microbiology 2023Quote: ... they were washed 3 times with 1M sorbitol and resuspended in RPMI 1640 broth containing protease inhibitor cocktail (Sigma-Aldrich) to final concentration of 3×108 cells/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were subsequently washed 3 × 5 min in 1x TBS/ 0.1% Tween-20 and developed using Immobilon Western HRP Substrate (Millipore, WBKLS0S00) and imaged using the Azure C300 ...
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Immunology 2022Quote: ... a catheter was carefully inserted and the infrarenal part was perfused with sterile isotonic saline containing type I Porcine Pancreatic Elastase (2.5-3 U ml, Sigma-Aldrich) for 5 min at 120 mm Hg ...
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bioRxiv - Immunology 2023Quote: ... Larvae were anaesthetized in zebrafish water supplemented with 200 μg/ml tricaine (Ethyl 3-aminobenzoate methanesulfonate, MS-222 Sigma #A5040) before any manipulation (infection or imaging ...
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bioRxiv - Genomics 2023Quote: ... Twenty-four hours after transduction cells were recovered for another 24 hrs and then MCF10A were selected in 3 μg/ml puromycin dihydrochloride (Sigma) for 48 hrs or 10 μg/ml blasticidin (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
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bioRxiv - Immunology 2023Quote: ... capsules were attached to the back of mice using a warmed adhesive solution made from 3 parts gum rosin (Millipore- Sigma) and 1 part beeswax (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The peak absorbance (A280) fraction was collected and concentrated to 22 mg/mL using a 3 kDa MWCO concentrator (Millipore) before flash freezing in liquid nitrogen.
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3 times for 15 min in PBS and incubated in 0.0001% biotin-tyramide (Sigma Aldrich, SML2135-50MG) and 0.003 % H2O2 in 0.05 M borate buffer ...
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bioRxiv - Physiology 2023Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). For all variants in pET28b except CLC ...
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bioRxiv - Physiology 2023Quote: Purified GS proteins were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). Proteins were dialyzed at 25 °C for 4 hours against 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9 ...
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bioRxiv - Microbiology 2023Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was first precoated with fibronectin (3 µg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report73 ...
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bioRxiv - Biochemistry 2023Quote: ... The slides were washed again carefully with PBS 3 x 5 mins and the coverslips were mounted onto glass microscope slides with Fluoroshield with DAPI (Sigma) and sealed with clear nail varnish ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting conjugated antibodies are then washed for 3 times in high salt PBS (0.9 M NaCl) in a 50 kDa centrifugal column (Sigma, UFC505096), buffered with at least 30% v/v Candor Antibody Stabilizer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were spun down and the supernatant was passed through a C8 membrane (3 M Empore™, Sigma-Aldrich), to remove any residual beads ...
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bioRxiv - Cell Biology 2023Quote: ... Dissected gut segments were inverted inside out and incubated in DMEM supplemented with 3 mM DTT (Sigma Life Science, D9779), 1 mM EDTA (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... TOE1 was depleted in HEK 293T-REx-derived TOE1-degron cells12 by incubation with 600 μM auxin hormone Indole-3-Acetic Acid (IAA, Sigma) for 8 hours ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 times for 5 min in PBS and blocked with 3% BSA in PBS-Triton 0.01% (Triton X-100, Sigma, T8787) for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were washed 3 times in PBS-T (phosphate buffered solution + 0.6% Triton-X 100) (Sigma-Aldrich, Cat. No. X100). PBS-T was removed and VECTASHIELD Mounting Media (Vector Labs ...
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bioRxiv - Pathology 2023Quote: ... Nuclear fast red solution was prepared using 0.1 g nuclear fast red powder in 100 mL distilled water with 5 g Al2(SO4)3 (Sigma-Aldrich). Deparaffinized slides were washed with distilled water and then incubated with the Alcian blue solution for 30 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... mice were fasted for four hours before oral gavage of 600 mg/kg FITC–3-5-kDa dextran (Sigma, FD4). Serum was collected six hours after gavage as well as control mice without gavage and analyzed using a Synergy HT plate reader (Bio-Tek ...