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Citations for Millipore Sigma :
6501 - 6550 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Aliquots of 3 µl of between 3-6 mg/ml of Toll5A-Spz1C gel-filtered complex mixed with 8 mM CHAPSO (final concentration, Sigma) were applied to the grids ...
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bioRxiv - Immunology 2022Quote: ... a catheter was carefully inserted and the infrarenal part was perfused with sterile isotonic saline containing type I Porcine Pancreatic Elastase (2.5-3 U ml, Sigma-Aldrich) for 5 min at 120 mm Hg ...
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bioRxiv - Immunology 2022Quote: ... Samples were permeabilized in 0.1% Triton-X-100 for 3 min and then incubated in a blocking solution consisting of 10% FBS (Sigma-Aldrich), 0.01% sodium azide (Sigma-Aldrich) ...
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Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capturebioRxiv - Synthetic Biology 2023Quote: ... To the bead suspension was added 0.5 mL of 750 mM of N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) in water (Sigma-Aldrich) and the mixture was incubated for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: Yp strains were induced to upregulate expression of the T3SS and associated effectors by taking 100 μL of stationary phase overnight cultures and adding to 3 mL 2x YT media containing 20mM sodium oxalate (Sigma) and 20mM MgCl (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed in TBS (3 washes for 5 min each) and then treated with 0.25% H2O2 (Cat#216763, Sigma-Aldrich), in TBS for 3 min ...
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bioRxiv - Microbiology 2023Quote: The small interfering RNAs (siRNAs) against NLRP3 (siNLRP3) (5’-UGCAAGAUCUCUCAGCAAA-3’) and the corresponding control siRNAs (siControl) (5’-UUCAAUAAAUUCUUGAGGU-5’) were synthesized from Sigma. The siGenome smart pool siRNAs and siON Target plus siRNAs were purchased from Dharmacon and are composed of a pool of four siRNAs ...
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bioRxiv - Cell Biology 2023Quote: ... In vitro excision of Nr3c1 exon 3 was achieved by treating primary myoblasts with 4-OH-tamoxifen (Sigma Aldrich, H7904).
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bioRxiv - Bioengineering 2023Quote: ... and 5 μm sections were taken before being baked for 3 hours at 60°C and stained with hematoxylin and eosin (H&E, Sigma) or Masson’s trichrome (Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting conjugated antibodies are then washed for 3 times in high salt PBS (0.9 M NaCl) in a 50 kDa centrifugal column (Sigma, UFC505096), buffered with at least 30% v/v Candor Antibody Stabilizer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The beads were spun down and the supernatant was passed through a C8 membrane (3 M Empore™, Sigma-Aldrich), to remove any residual beads ...
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bioRxiv - Cell Biology 2023Quote: ... Dissected gut segments were inverted inside out and incubated in DMEM supplemented with 3 mM DTT (Sigma Life Science, D9779), 1 mM EDTA (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... The slides were washed again carefully with PBS 3 x 5 mins and the coverslips were mounted onto glass microscope slides with Fluoroshield with DAPI (Sigma) and sealed with clear nail varnish ...
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bioRxiv - Molecular Biology 2023Quote: ... TOE1 was depleted in HEK 293T-REx-derived TOE1-degron cells12 by incubation with 600 μM auxin hormone Indole-3-Acetic Acid (IAA, Sigma) for 8 hours ...
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bioRxiv - Physiology 2023Quote: Fractions were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). For all variants in pET28b except CLC ...
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bioRxiv - Physiology 2023Quote: Purified GS proteins were assessed by SDS-PAGE and pooled for dialysis in 3.5 kDa MWCO dialysis tubing (SpectraPor 3 Dialysis Membrane, catalog 132724, Sigma Aldrich). Proteins were dialyzed at 25 °C for 4 hours against 25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... larvae were washed 3 times in PBS-T (phosphate buffered solution + 0.6% Triton-X 100) (Sigma-Aldrich, Cat. No. X100). PBS-T was removed and VECTASHIELD Mounting Media (Vector Labs ...
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bioRxiv - Microbiology 2023Quote: ... the bottom channel of a polydimethylsiloxane (PDMS) device was first precoated with fibronectin (3 µg/ml, Sigma-Aldrich, Cat# F1141). The microfluidic device was generated according to our previous report73 ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed 3 times for 15 min in PBS and incubated in 0.0001% biotin-tyramide (Sigma Aldrich, SML2135-50MG) and 0.003 % H2O2 in 0.05 M borate buffer ...
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bioRxiv - Immunology 2023Quote: ... Larvae were anaesthetized in zebrafish water supplemented with 200 μg/ml tricaine (Ethyl 3-aminobenzoate methanesulfonate, MS-222 Sigma #A5040) before any manipulation (infection or imaging ...
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bioRxiv - Pathology 2023Quote: ... Nuclear fast red solution was prepared using 0.1 g nuclear fast red powder in 100 mL distilled water with 5 g Al2(SO4)3 (Sigma-Aldrich). Deparaffinized slides were washed with distilled water and then incubated with the Alcian blue solution for 30 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed 3 times for 5 min in PBS and blocked with 3% BSA in PBS-Triton 0.01% (Triton X-100, Sigma, T8787) for 1h ...
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bioRxiv - Microbiology 2023Quote: ... The peak absorbance (A280) fraction was collected and concentrated to 22 mg/mL using a 3 kDa MWCO concentrator (Millipore) before flash freezing in liquid nitrogen.
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bioRxiv - Neuroscience 2023Quote: ... Culture supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Immunology 2023Quote: ... capsules were attached to the back of mice using a warmed adhesive solution made from 3 parts gum rosin (Millipore- Sigma) and 1 part beeswax (Thermo Scientific ...
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bioRxiv - Genomics 2023Quote: ... Twenty-four hours after transduction cells were recovered for another 24 hrs and then MCF10A were selected in 3 μg/ml puromycin dihydrochloride (Sigma) for 48 hrs or 10 μg/ml blasticidin (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 3 dpf larvae were anesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The corresponding concentration of bacteria was injected into the corresponding part of the zebrafish ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 100 µL of elution buffer supplemented with 200 µg/mL 3× Flag-peptide (Sigma-Aldrich, #F4799) and stored at -80◦C until usage ...
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bioRxiv - Immunology 2023Quote: ... BMDMs were primed with 100 ng/mL LPS for 3 hours followed by 2.5 mM ATP treatment or 5h 10uM IKKi (BMS-345541, Sigma-Aldrich) treatment ...
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bioRxiv - Cell Biology 2023Quote: ... 3 – 6 mg of cell extract and 20 μl of α-Flag M2 affinity bead suspension (Sigma, catalog no.: A2220) were used ...
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bioRxiv - Developmental Biology 2023Quote: ... in PBS at +4⁰C in the shaker and then 3-4 min with a pancreatin-trypsin (2.5 mg/ml pancreatin [P3292; Sigma Aldrich] and 22.5 mg/ml trypsin dissolved in Thyrode’s solution pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... and then washed with PB (3 × 5 min; RT) and finally mounted on gelatinised slides and coverslipped with Mowiol DABCO (Sigma). For brightfield microscopy ...
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bioRxiv - Neuroscience 2022Quote: ... mito-tempol[23] (25 μg, Sanbio) and oligodeoxynucleotide (3 μg/μl day 4, 5 and 6 after carrageenan, Sigma-Aldrich), were performed under light isoflurane anesthesia as described.[8a ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Molecular Biology 2022Quote: ... blood was collected via cardiac puncture from anesthetized mice (100 mg/kg ketamine and 3 mg/kg xylazine, Sigma-Aldrich). Whole blood aliquots were stored in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: Tissues or cells were homogenized in Pierce IP Lysis buffer supplemented with protease inhibitor cocktail 3 (Merck Millipore, Darmstadt, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS and 3-4 min at room temperature with a pancreatin-trypsin mixture (2.5mg/ml pancreatin [P3292; Sigma Aldrich] and 22,5mg/ml trypsin in Thyrode’s solution pH 7.4) ...
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bioRxiv - Cancer Biology 2024Quote: ... About 3x106 cells were immunoprecipitated using 10 µg of a monoclonal SF3B1 antibody (clone 16, MBL D221-3) or 10 µg of the monoclonal U2AF2 antibody (U4759, SIGMA).
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bioRxiv - Cell Biology 2024Quote: ... The flat mount was then rinsed with HBSS and incubated in blocking buffer made with HBSS with 5% bovine serum albumin (Catalog #BP9703-100) (W/V) and 3% Triton X-100 (Sigma) (V/V ...
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bioRxiv - Plant Biology 2024Quote: ... meliloti 1021 (pXLGD4) strain were stained using 5-bromo-6-chloro-3-indolyl-β-d-galactopyranoside (Magenta-Gal, Sigma-Aldrich), according to the protocol described previously by Jarzyniak et al ...
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bioRxiv - Plant Biology 2024Quote: ... a 188 bp cDNA fragment of MtABCG40 5’UTR or a 200 bp cDNA fragment of MtLOG3 3’UTR were amplified with KOD Hot Start DNA Polymerase (Novagen) and cloned into pDONR/Zeo vector (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... HeLa cells were transfected with U6-gDNA (5’-AAAGACGTCCCTAACAAGT-3’; clone ID es: HSPD0000063884): CMV-eCas9-2a-tGFP (Sigma-Aldrich). 48h after transfection ...
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Microbiology 2024Quote: ... a total of 50 mL Aureispira culture grown in ASWM were pelleted, resuspended in 3 mL of lysis buffer (150 mM NaCl, 50 mM Tris-HCl, 0.5× CellLytic B (Sigma-Aldrich), 1% Triton X-100 ...
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bioRxiv - Biophysics 2024Quote: ... The remaining iron nitrate salt was allowed to cool then quantified using a colorimetric iron assay kit with 3 technical replicates for each cell type (MAK025, Sigma). The total iron from 3 technical replicates was averaged then divided by the number of cells to obtain calculated pg Fe/cell.
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bioRxiv - Plant Biology 2024Quote: ... 2.5 cm sections were dissected from excised leaves (cut 3 cm from the leaf tip) and placed on 12 cm2 0.5% agar (type M; Sigma-Aldrich) Petri plates ...
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bioRxiv - Microbiology 2024Quote: ELISAs were used to confirm a subset of cytokines identified in the antibody arrays (CCL2, CX3CL1, and Galectin-3 rat ELISA kits, Sigma) using apical or basal supernatants from individual Transwell® inserts from the same experiment than the multiplex array.
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bioRxiv - Neuroscience 2024Quote: ... cells were cultured in 0.4 µm or 3.0 µm pored Transwell inserts and fluorescein isothiocyanate (FITC)-conjugated dextran molecules of 3– 5 kDa or 150 kDa (Sigma) were added at 0.5 mg/ml to the top chamber of the Transwell insert for 24 h ...
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bioRxiv - Genomics 2023Quote: ... with 10% charcoal-stripped FBS (#100-119, GemBio) for 3 days and then were stimulated with 10nM dihydrotestosterone (DHT) (A8380, Sigma) for 0.5 ...