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Citations for Millipore Sigma :
6901 - 6950 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... Carboxyl groups on the photo-activated regions were activated with 50 µL/well of 0.05 g/mL N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (Sigma-Aldrich, 03450) and N-Hydrosuccinimide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... Frozen tissue was transferred to a pre-chilled 3 ml Kimble dounce homogenizer filled with 4ml Nuclei Lysis Buffer (Sigma, NUC101), supplemented with RNAase inhibitors (10mg/ml ...
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bioRxiv - Genomics 2021Quote: ... Degradation of the AID-tagged RAD21 was induced by the addition of 500 μM (final concentration) indole-3-acetic acid (IAA/auxin; Sigma Aldrich) during media change 24 hours prior to imaging if applicable.
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bioRxiv - Pathology 2021Quote: ... The tissue was blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... Large aggregates were removed by filtering through a 100-kDa filter followed by concentrating the solution in a 3-kDa filter (Millipore Sigma). To create fibrils ...
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bioRxiv - Biochemistry 2021Quote: ... Any large aggregates were removed by filtering the solution through a 100-kDa filter and then concentrating the solution using a 3-kDa filter (Millipore Sigma). Samples were diluted to 70 μM ...
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bioRxiv - Cell Biology 2020Quote: ... 5 % CO2 at 37 °C were seeded on Seahorse microplates pre-coated with 3 μg/mL collagen I (C9791, Sigma Aldrich) in 0.1 M acetic acid and allowed to adhere for at least 12 h ...
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bioRxiv - Cell Biology 2020Quote: ... washed twice with 500 µL PBS and re-suspended in 500 µL of PBS containing ∼3 mg/mL Biotin-NHS (Sigma B1022). Cells were incubated for 30 min on a rotating wheel at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... injected embryos were manually dechorionated and anesthetized by incubation with 0.14 mg/ml Tricaine (Ethyl 3-aminobenzoate methanesulfonate; A5040, Sigma-Aldrich, USA).
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bioRxiv - Bioengineering 2021Quote: A1 reactive astrocytes were generated by culturing the purified astrocytes on PDMS coated tissue culture plates and then treating for 24 h with IL-1α (3 ng/ml, Sigma, I3901), TNF-α (30 ng/ml ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Evolutionary Biology 2021Quote: An overnight culture of Bacillus thuringiensis Berliner (ATCC 55177) was prepared from a glycerol stock and grown at 30°C in Nutrient Broth #3 (NB, Sigma Aldrich). An aliquot of overnight culture was brought to an OD of 0.5 in fresh NB ...
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bioRxiv - Molecular Biology 2021Quote: ... n = 3 for each group) were intraperitoneally injected with lipopolysaccharide (LPS; Escherichia coli 0111:B4, Sigma-Aldrich, St Louis, MO, USA), polyinosinic:polycytidylic acid [poly(I:C) ...
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bioRxiv - Microbiology 2020Quote: ... The pellet was resuspended in 200 μL lysozyme from a 3 mg/mL stock solution and 10 μL lysostaphin from a 10 mg/mL stock solution (Sigma-Aldrich). Cells were incubated at 37°C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... The resin was washed 5 times and transferred to a spin column with 40 μl of 3 X Flag peptide (Sigma, #F4799) for 1 h at 4°C on a rotator ...
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bioRxiv - Microbiology 2021Quote: ... The exudate metabolites were separated on a SeQuant ZIC-pHILIC HPLC column (2.1 × 100 mm, 3 μm) (Milllipore Sigma, Burlington, MA) using acetonitrile with 0.1% formic acid as solvent B and water with 0.1% formic acid as solvent A at a flow rate of 400 μL min−1 and the following linear gradient extending over 14 min ...
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bioRxiv - Microbiology 2021Quote: ... was from Thermo Fisher Scientific (Massachusetts, USA). 3-MA (Cat no. M9281) and Anti-HA antibody (Cat no. A190-101) was from Sigma (Missouri, USA). Nontargeting siRNA (Cat no ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... followed by co-incubation with an excess of HeLa cells (recipient) for 3 h in the presence of 50 μg/ml cycloheximide (Sigma, # C104450), to prevent further protein synthesis in the heterokaryons ...
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bioRxiv - Immunology 2020Quote: ... and 2.5 ng/mL TNF-α for 3-5 days and subsequent differentiation in RPMI (Sigma, St Louis, Mo; +10% FCS) either with 100 ng/mL GM-CSF ...
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bioRxiv - Bioengineering 2021Quote: ... Equal amounts of protein were subject to SDS-PAGE and blotted with anti-Rho antibody (3 μg/mL, #05-778, EMD Millipore) or anti-GAPDH antibody (1:1000 dilution ...
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bioRxiv - Genomics 2021Quote: ... The cells were rinsed and kept in the blocking buffer before photo-bleaching and submerged in 3-5’-diaminobenzidine (DAB) solution (Sigma Aldrich) during photo-bleaching on the cold stage.
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bioRxiv - Genomics 2021Quote: ... were selected and mono-nucleosomal DNA fragments were purified from 3% low melting-temperature agarose gels and treated with β-agarase (Sigma) for sequencing.
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bioRxiv - Cancer Biology 2021Quote: ... The depleted samples was collected in a 20 mL flowthrough fraction and the three independent depletions were pooled and concentrated in an Amicon Ultra Centrifugal Filter Unit (3 kDa cutoff; Millipore UFC900324) and buffer exchanged three times with 1x Urea buffer (6 M Urea ...
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bioRxiv - Genomics 2020Quote: ... the auxin-inducible degron was induced by adding 500 μM indole-3-acetic acid (IAA, chemical analog of auxin) (Sigma, I5148) to the media ...
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bioRxiv - Molecular Biology 2023Quote: ... Freshly resected left ventricular tissue (~3-5 mg per sample) was pulled into fibers and permeabilized in the presence of saponin (50 μg/mL, Sigma, 47036) 30 min at 4°C in BIOPS buffer containing the following ...
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bioRxiv - Systems Biology 2023Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1 μm centrifugal filter (Millipore ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Experiments were designed to explore the effects of directly applying PCA (Sigma Aldrich, ref. P-5630, CAS number 99-50-3) to the brain (15 mg/kg dissolved in artificial cerebrospinal fluid –ACSF-with 1% dimethyl sulfoxide -DMSO ...
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bioRxiv - Neuroscience 2023Quote: ... for co-immunoprecipitation analysis and into 96-well cell culture plates (3×104 cells/well) coated with 0.01% poly-L-lysine (Sigma-Aldrich, #P9404) for ELISA ...
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bioRxiv - Cell Biology 2023Quote: ... The AAV was concentrated by centrifugation at 2000g for 3-5min at 4°C using an 100k Amicon Ultra Centrifugal Filter Unit (Millipore #UFC810024). After centrifugation ...
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bioRxiv - Immunology 2023Quote: ... Then the slides were washed in running tap water for 3 min before counterstaining in Hematoxylin Solution (Sigma-Aldrich, Cat# 105174) for 10 s.
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bioRxiv - Molecular Biology 2023Quote: ... 500k Day 25 hiPSC-CMs and 100k human Hs27a stromal cells were mixed with 3 U/mL thrombin from bovine plasma (Sigma, T4648) and 5 mg/mL bovine fibrinogen (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... in PBS 1X at RT for 40 min and incubated with a blocking buffer containing 3% bovine serum albumin (BSA, Sigma Aldrich) in PBS 1X RT for 40 min ...
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bioRxiv - Microbiology 2023Quote: ... the wells were filled with 200 µl fresh M63 media with or without 3 µM of human transferrin (Sigma-Aldrich #T1147) and incubated at room temperature for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... a catheter was inserted and the infrarenal part was perfused with sterile isotonic saline containing type I porcine pancreatic elastase (2.5-3 U/ml Sigma Aldrich, #E1250) or 0.9% NaCl (sham surgery ...
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bioRxiv - Evolutionary Biology 2023Quote: ... for 3 hours and then incubated overnight at 4°C in block with primary antibodies: mouse anti-acetylated tubulin (Sigma, T6793) at 1:250 or rabbit anti-phosphorylated Smad1/5/8 (Cell Signaling ...
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bioRxiv - Neuroscience 2023Quote: ... samples were washed with vDISCO washing solution for 3 hours and 3DISCO clearing60 with 50-70-90-100-100% tetrahydrofuran (THF) (Sigma, 186562), 1h each ...
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Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... Anhydrous 100% ethanol solution was prepared by adding 15 g of molecular sieve beads (Sigma 208582, 3 Å, 8-12 mesh) to 500 mL 100% molecular grade ethanol (Sigma E7023) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... diluted in corn oil (CO, used as vehicle) at 3 mg/kg for 4 days while CO mice received corn oil only (Sigma Aldrich). One cage of n=6 mice per group was used ...
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bioRxiv - Physiology 2023Quote: ... and then incubated overnight at 4°C in primary antibodies diluted in 50% blocking buffer / 50% Tris buffered saline (bioWORLD #42020056–3) + 0.1% TWEEN (Sigma-Aldrich #P9416) (TBS-T) ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.74 mmol of 3 or 4 azidoacids were stirred for 10 minutes at 40°C in minimal dimethylformamide (Sigma-Aldrich, 319937). The solution was then added with 0.74 mmol of DCC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Regeneration of the chip surface was achieved by brief exposure (30 s) to a solution containing 3 M solution of KSCN (Sigma-Aldrich). BIAevaluation software (version 4.1.1 ...
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bioRxiv - Microbiology 2023Quote: ... 20 mM glucose final concentration; 10 g tryptone [Fisher Bioreagents, BP1421], 5 g yeast extract [Fisher Bioreagents, BP1422], 3 g K2HPO4 [Sigma-Aldrich, P3786] ...
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bioRxiv - Immunology 2023Quote: ... mice were orally gavaged with 150 μL of an 80 mg/mL solution of 3-5 kDa-FITC-Dextran (Sigma-Aldrich) in PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... autologous plasma samples were taken from the top layer and the “buffy coat” was extracted and washed 3 times with 1x PBS (Sigma-Aldrich) at 300 x g ...
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bioRxiv - Genetics 2023Quote: BCL11A enhancer DHS +58 and +55 core regions were amplified with KOD Hot Start DNA Polymerase (EMD-Millipore, cat# 71086-3) and corresponding +58 Sanger primers ...
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bioRxiv - Biophysics 2023Quote: ... solution for 3 hours at 4°C under gentle agitation and then washed it three times in TBS/Tween20 (TBST) buffer (Sigma Aldrich). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Sub culturing was done once in 3-4 days and involved pelleting and washing cells with PBS followed by incubation with accutase (Sigma-Aldrich) and repeated pipetting in NSM medium to dissociate the spheroids ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... cells were washed twice with phosphate-buffered saline (PBS) and fixed by incubation in a phosphate buffer containing 3% paraformaldehyde (Sigma-Aldrich) for 25 min ...