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Citations for Millipore Sigma :
6751 - 6800 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... 1 μl lipids and 3 μl standards (30 μg mono-, di-, triglyceride mix, SUPELCO 1787-1AMP, plus 30 μg cholesteryl palmitate, SIGMA C6072) were spotted onto the plates using TLC spotting capillaries ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 µL of cells were transferred into 100 µL of staining solution (Phosphate Buffer Saline + 3 µM propidium iodide (Sigma-Aldrich) + 200 nM YP (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The plates were incubated for two hours at RT and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate solution (Sigma-Aldrich, USA) was added for colour development ...
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bioRxiv - Developmental Biology 2021Quote: ... The proximal forelimbs of embryos were dissected (see Figure 3) and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37° for approximately 5min ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Microbiology 2022Quote: ... the samples were infiltrated with 70% Epon in Ethanol before two incubations with 100% Epon and the final polymerization was carried out in Epon supplemented with 3% silver flakes (Sigma-Aldrich) and 3% (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... and 3% hydrochloric acid followed by sample pooling via centrifugation at 1,500 rpm for 2 minutes to the proteoCHIP funnel part for direct injection or transferred to 0.2 mL PCR-tubes coated with 1e-3 % Poly(ethylene glycol) (95172-250G-F, Sigma-Aldrich, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Each well of 6-well cell culture treated plates coated with 0.1% Poly-L-ornithine (3 ug/mL; Sigma Aldrich, P4957) were seeded with ∼750K cells and maintained with sterile-filtered neurobasal medium (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... permeabilized in PBS containing 0.5% Triton X-100 (Tx-100; Nacalai Tesque) for 30 min and blocked in PBS containing 3% bovine serum albumin (BSA; Sigma-Aldrich) and 0.05% Tx-100 for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... 6M NaCl; 10% SDS; 0.5M EDTA; Triton-X 100; Phosphatase Inhibitor #3, Roche, #05-892-970-001; Protease Inhibitor, Sigma-Aldrich, #P0044) by homogenizing 3 x 20s and sonicating on ice for 15s at 40Hz ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed 3 times with PBS-G and blocked in PBS-G with 0.5% fish skin gelatin (Sigma-Aldrich, G7041) for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were washed in PBS at least 3 times for 4 hrs and then incubated in Histodenz-Triethanolamine solution (about 80% Histodenz D2158 (Sigma-Aldrich), 11.5 mM PB ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2020Quote: ... Infected cells were treated with the indicated concentrations of caffeine (RyR, 0-200 mM) or IP3 (IP3R, 0-3 µM) (Sigma-Aldrich). A Synergy (BioTek ...
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bioRxiv - Cell Biology 2020Quote: ... BI-D1870 (called ‘p90RSKi’ in this study, Cayman 15264), BIO-6-bromoindirubin-3′-oxime, aka BIO (called ‘GSK3i2’ in this study, (Sigma B1686), BI 2536 (called ‘PLKi’ in this study ...
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bioRxiv - Plant Biology 2020Quote: ... from Streptomyces avermitilis (Cat. #PRO-E0233, 27.5 units/mg; PROZOMIX, Haltwhistle, UK) was desalted with 3 kDa centrifugal filters (Merck Millipore, Tullagreen, IRL) and diluted to 50μg/mL in 20mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... The absorbance of the reaction mixture was measured at 500 nm and the total flavan-3-ol content was calculated from a calibration curve prepared using (+)-catechin (Sigma-Aldrich) and expressed as milligrams per gram of dried extract ...
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bioRxiv - Physiology 2021Quote: ... 2.5 CaCl2 and 10 glucose) to which was added 3 mg/mL collagenase I (Sigma Chemical Co., St Louis, MO, USA) and 1 mg/mL trypsin inhibitor (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... Single colonies were collected and used to inoculate a 3-mL YEB (yeast extract broth) starter containing 10 µg/mL cephalexin hydrate (Cp, Sigma-Aldrich). Starters were grown overnight at 28°C with 185-200 rpm shaking and then used to inoculate 500 mL of PSB (peptone sucrose broth ...
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bioRxiv - Genetics 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, MO, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Genetics 2021Quote: The CTCF protein was depleted in CTCF-AID mESCs by adding 500 μM of indole-3-acetic acid (IAA, a chemical analog of Auxin; Sigma-Aldrich) to the medium for 24 hrs ...
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bioRxiv - Microbiology 2020Quote: ... or mock nucleofected in the presence of one of the following inhibitors (auto= autophagy inhibitor, 3-methyladenine, authophagy inhibitor-M9281, Sigma Aldrich; apo= apoptosis inhibitor ...
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bioRxiv - Developmental Biology 2020Quote: ... The common bile duct was clamped at the duodenum and a 30G needle was used for perfusion of 3 mL Collagenase XI (1000 U/mL, Sigma-Aldrich) in Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Immunology 2021Quote: ... larvae were anesthetized in E3 media without methylene blue supplemented with 0.16 mg/ml Tricaine (MS222/ethyl 3-aminobenzoate; Sigma-Aldrich-Aldrich).
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bioRxiv - Neuroscience 2020Quote: ... followed by primary antibody incubation in 10% sheep serum at 4°C for 3 days (Anti-SCN5A antibody produced in rabbit (Sigma SAB2107930) 1:1000 ...
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bioRxiv - Genomics 2020Quote: Zebrafish embryos/larvae were euthanized by prolonged immersion in 200-300 mg/L tricaine (MS222; ethyl-3-aminobenzoate methanesulfonate, #E10521-10G, Sigma-Aldrich). Whenever necessary the chorion was removed ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissues were then washed with wash buffer and blocked for 1 hour at room temperature with 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... The first antibody cocktail was prepared in 1x TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich) and filtered through a 0.1μm centrifugal filter (Millipore ...
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bioRxiv - Neuroscience 2020Quote: ... free-floating VTA brain sections were incubated for 1 hour at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503) (vol/vol ...
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bioRxiv - Bioengineering 2021Quote: ... The primary antibody buffer was 3% horse serum and 5% DMSO dissolved in 1X PBS with 0.2% Tween-20 (Sigma Aldrich, P2287) and 10 μg/mL heparin (Sagent Pharmaceuticals ...
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bioRxiv - Bioengineering 2021Quote: ... and then seeded atop the Matrigel-coated surfaces at a density of either 1×105 cells/cm2 (96-well plates) or 3×105 cells/cm2 (hydrogels) in CMGF+ medium supplemented with 10 µM Y-27632 (EMD Millipore) for 24 h to form monolayers ...
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bioRxiv - Bioengineering 2020Quote: ... 15 μL droplets of cell-hydrogel mix were placed on the surface of elastomeric membranes that underwent a silanization process through a nitric acid and (3-Aminopropyl)triethoxysilane (Sigma-Aldrich) treatment ...
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bioRxiv - Bioengineering 2020Quote: ... at 20-25×105 cells/dish and cultured in primary reprogramming medium (StemMACS iPS-Brew XF medium (iPS-Brew; Miltenyi Biotech) supplemented with 3 μM CHIR99021 (Sigma-Aldrich) and 10 μM SB431542 (Selleckchem ...
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bioRxiv - Biochemistry 2020Quote: ... the lyophilized pellets of all samples were re-suspended in 12 ml MilliQ water and the proteins were concentrated on 3 kDa cutoff Amicon® Ultra 15 centrifugal filter units (Merck-Millipore) 5,000 x g at 4°C to reach a final volume of 0.5 ml ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were quickly washed 3 times with TBS/T (0.1% Tween20, Sigma #P5927; 20mMTris-HCL pH 7.6, T7943, Sigma, 150mM NaCl, Sigma) then stained with Ponceau S (PoncS ...
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bioRxiv - Biochemistry 2020Quote: ... The instrument response function (IRF) of the system was measured using a dilute aqueous solution (3% w/w) of LUDOX AM colloidal silica (Sigma-Aldrich). The full-width half maxima (FWHM ...
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bioRxiv - Biochemistry 2020Quote: ... 200 μl plasma from a single animal was diluted in 15 ml PBS and concentrated 30-fold using 3 kDa filter tubes (Millipore UFC900324) at 4,000 rpm for 1.5 h ...
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bioRxiv - Neuroscience 2020Quote: ... cell death caused by drugs were detected by double staining the cells with 5 μl Hoechst 33342 (23491-52-3, Biotech, China) and Propidium iodide (PI) (P4170, Sigma, USA) for 15 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... mechanically dissociated cortices from P1-3 Wistar rats were cultured in poly-D-lysine (PDL)-coated (10 μg/mL; Sigma P7886) tissue culture plates (diameter 10 cm ...
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bioRxiv - Neuroscience 2020Quote: ... Stages VI oocytes from Xenopus tropicalis anesthetized by 0.4% ethyl 3-aminobenzoate methanesulfonate (MS-222)(Sigma-Aldrich; St. Louis, Missouri, USA) were prepared by treatment with 0.1 mg/ml collagenase (Worthington ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were blocked at room temperature with either 5% (w/v) non-fat milk or 3% (w/v) BSA (Sigma- Aldrich) in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... Microstructures were then generated using high-resolution chrome masks (JD phototools, UK) and 365nm UV exposure (UV KUB 3, Kloe, France) followed by PGMEA (Sigma-Aldrich) development ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on glass coverslips and bone slices for 3 days were fixed for 10 min in a 3.7% (wt/vol) paraformaldehyde (Sigma Aldrich 158127) solution containing 0.25% glutaraldehyde (Electron Microscopy Sciences 16220 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
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bioRxiv - Physiology 2021Quote: ... Samples were washed in 60% isopropanol and incubated for 30 minutes at 37°C in a freshly filtered solution of 3 mg/ml oil red O (O0625, Sigma Aldrich) in 60% isopropanol ...
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bioRxiv - Plant Biology 2021Quote: Pellets of isolated EV fractions were washed twice with 50 mM ammonium bicarbonate buffer using Amicon Ultra 3 kDa cut-off centrifugal filters (Merck Millipore Ltd), and total protein concentration was estimated using Quick Start Bradford Protein Assay (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... transduction of cells was performed with a multiplicity of infection equal to 3 in the presence of 8 µg/ml Polybrene (H-9268; Sigma-Aldrich). Cells were then selected with 2.5 µg/ml puromycin for 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... DLD1-wildtype and DLD1-NXF1-AID cells were pretreated for 0-8 hrs with 0.5 mM synthetic auxin (3-indoleacetic acid, Sigma, in ethanol), prior to addition of 250 nM NVP-2 ...