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Citations for Millipore Sigma :
6801 - 6850 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The wells were further blocked with 15 µl of 10 µM biotin in 3% skim milk in PBST (Sigma, cat# 1.15363.0500) for ten min at R.T ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2020Quote: ... and the 3 lines that germinated poorly after stratification showed >97% germination when sown on plates containing 10μM Gibberellin A4 (SIGMA ALDRICH, G7276).
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bioRxiv - Microbiology 2020Quote: ... Transverse sections (6 µm) were sectioned and mounted on silane-covered (TESPA, 3-aminopropyl-triethoxysilane, Sigma-Aldrich, St. Louis, Missouri, USA) glass slides ...
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bioRxiv - Microbiology 2020Quote: ... cells were plated on 24-well plates at 3×105 cells/well with 0.1 µg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich #P1585) to promote differentiation ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were washed three times with the protein elution buffer (20 mM Tris-HCl, 150 mM NaCl, 10% glycerol) and eluted by adding 3× FLAG peptide (Sigma-Aldrich) in 60 µL of the protein elution buffer following the manufacturer’s instruction.
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bioRxiv - Plant Biology 2021Quote: ... sucrose (#S/8600/60, Fisher) and 100 μM 3’,5’-dimethoxy-4’-hydroxyacetophenone (acetosyringone) (#115540050, Acros Organics, dissolved in dimethyl sulfoxide (DMSO) (#D8418, SIGMA)) ...
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bioRxiv - Neuroscience 2020Quote: ... N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 0.3 µg/0.5 µL/hemisphere; n = 9; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Microbiology 2021Quote: ... Culture supernatants were prepared by centrifuging cultures 5 min at 10000 × g and concentrating the cell free supernatant approximately 10 times with 3 kDa molecular weight cut-off Amicon Ultra-15 centrifugal concentrators (Merck Millipore, Germany). The concentrated supernatant was then transferred to fresh tubes for protein content determination ...
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bioRxiv - Microbiology 2021Quote: ... Parasites were harvested by scraping the infected host-cell monolayers and passing host cells through 28g needles (3x) before removing host-debris through filtration (3 μm pore size, Millipore, TSTP04700). The flow-through was collected on ice and filters were rinsed with additional ice-cold PBS to maximize the yield of parasites ...
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bioRxiv - Physiology 2021Quote: ... Sections were randomly selected for immunohistochemistry analysis using slides impregnated with 3% organosilane (Sigma Chemical Company®, St. Louis, MO, USA) in ethanol.
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bioRxiv - Microbiology 2021Quote: ... Virus supernatants were removed 7 h post-spin inoculation and cells were maintained in fresh growth medium for 48 h prior to selection in 3 μg/mL puromycin (Sigma-Aldrich). Stable cell lines were generated after 8 days of selection.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Major peaks were desalted by RP-HPLC using an Ascentis C18 column (3 μm, 4.6mm ID x 15 cm, Sigma-Merck, Germany) using peak-based collection (slope) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... membranes were washed (3×10 min) with TBS-T and incubated with the corresponding secondary antibody conjugated with peroxidase (Sigma-Aldrich) for 1 h at rt ...
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bioRxiv - Microbiology 2021Quote: ... The infected host cells were incubated for 7 min at 37°C with DMEM containing 3 μM of calcium ionophore A23187 (C7522, Sigma-Aldrich) prior to fixation with 4% PFA ...
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated onto collagen-coated plates in Opti-MEM for 3 hours and subjected to respective treatment conditions of either glucose (D-(+)-Glucose (Sigma Aldrich) or erythropoietin (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... glutaraldehyde buffered with 3× PHEM (120 mM PIPES, 50 mM HEPES, 20 mM EGTA, 4 mM magnesium chloride, all from Sigma-Aldrich) (Montanaro et al ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected at a density of 3 x 106 with equal amounts of heavy and light chain paired plasmids using polyethyleneimine (PEI, Sigma-Aldrich) at a ratio of 1:3 of total amount of plasmid to PEI ...
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bioRxiv - Cancer Biology 2020Quote: ... The effect of the anti-angiogenic compounds were measured using the 3-[4,5-dimethylthiazol- 2yl]-diphenyltetrazolium bromide (MTT) colorimetric assay (Sigma, Lyon, France) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin (Puromycin dihydrochloride ...
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bioRxiv - Cancer Biology 2020Quote: ... Next day cells were incubated for 24 h with the virus-containing supernatant diluted in culture medium and 3 µg/mL Hexadimethrine bromide (polybrene, Sigma Aldrich). Afterwards medium was changed and after additional 24 h the medium was changed to selection medium containing 1 µg/mL puromycin ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were cleaned using stage-tips preparations using 3 plugs of Empore polystyrenedivinylbenzene (SBD-XC) copolymer disks (Sigma Aldrich, MO, USA) for solid phase extraction.
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bioRxiv - Biophysics 2020Quote: ... were treated for 20 min at room temperature with 100 μl of Bind-Silane solution – a mixture of 3-(trimethoxysilyl)propylmethacrylate (7.15% by volume, Sigma-Aldrich, M6514) and acetic acid (7.15% by volume ...
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bioRxiv - Cell Biology 2020Quote: Unroofed cells were incubated in PBS containing 3% w/v bovine serum albumin (Fisher Bioreagents, BP9703) and 0.1% v/v triton X-100 (Sigma-Aldrich, T9284) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... All progenies of genetic crosses were verified by diagnostic PCR using KOD XTREME Hot Start DNA Polymerase (71975-3, EMD Millipore) or OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (M0488L ...
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bioRxiv - Cell Biology 2021Quote: ... Then, oocytes were transferred to a drop of chromosome spread solution (0.16% Triton-X-100, 3 mM DTT (Sigma-Aldrich, 43815), 0.64% paraformaldehyde in distilled water ...
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bioRxiv - Cell Biology 2021Quote: ... the carboxylic acid groups on the Dynabeads® were activated by a 30-minute incubation with N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide (EDC, cat. num. 39391, Sigma) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by blue native electrophoresis in 3-12% gradient gels (82, 83) using 10mg/ml Ferritin (404 and 880 kDa, Sigma F4503) and BSA (66 and 132kDa ...
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bioRxiv - Immunology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min and antigen retrieved using proteinase K (Sigma-Aldrich, P6556) in Tris-EDTA pH8 buffer for 4 min at room temp ...
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bioRxiv - Molecular Biology 2022Quote: Human hPSCs cultured on MEFs were harvested using collagenase IV as big aggregates and settled 3 times in washing media (DMEM [Thermo Fisher Scientific], 5% Newborn Calf Serum [Sigma] ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was resuspended in lysis buffer (100 mM Tris, pH 7.4, 150 mM NaCl, 5 mM imidazole, 3 mM βME, and protease inhibitor cocktail from Sigma-Aldrich: P8849), lysed by sonication ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were seeded into 6-well plates so that they reached 50-60% confluency on the day of infection and transduced at most 3 consecutive days with the viral stock in the presence of 8 µg/mL polybrene (Sigma-Aldrich). The viral solution was replaced with fresh culture medium ...
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bioRxiv - Biochemistry 2022Quote: ... The final proteins were concentrated in a prewashed Amicon Ultra-15 Centrifugal filter (molecular weight cut off 3 kDa; EMD Millipore), flash-frozen in liquid nitrogen and stored at -80 °C.
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells (a human lung epithelial cell line; ATCC HTB-55) were maintained in EMEM (Sigma-Aldrich, Cat# M4655-500ML) containing 20% FBS and 1% PS ...
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bioRxiv - Immunology 2022Quote: ... Cultures were stimulated with 20 ng/ml PMA for 3 hours and assessed with a mouse IFN-γ ELISA kit (Sigma-Millipore). For airway epithelial cell culture ...
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bioRxiv - Bioengineering 2022Quote: ... The cells were then incubated in a blocking buffer (5% normal goat serum, Vector; 5% normal donkey serum, Wako; 3% BSA, Sigma-Aldrich; and 0.1% Tween-20 ...
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bioRxiv - Cell Biology 2022Quote: ... the medium was changed to N2B27 medium34 with 3 μM CHIR99021 (CHIR) and 10 μM SB431542 (SB) (both from Sigma-Aldrich) and cultured for 5 days ...
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bioRxiv - Biophysics 2022Quote: ... Selected fractions (shown in Figure S4) were pooled and concentrated using Spin filters (3 kDa molecular weight cutoff, Amicon, Millipore Sigma) and stored at 4 °C before downstream characterizations ...
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bioRxiv - Biophysics 2022Quote: ... except that the cells were washed and incubated with 3 μM BioTracker™ 609 Red Ca2+ AM Dye (EMD Millipore #SCT021) in PBS for 30 min in a tissue culture incubator ...
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bioRxiv - Bioengineering 2022Quote: ... The retinal strips were sectioned (7–10 µm thick) and placed on microscope slides that were treated with 3-Aminopropyltriethoxysilane (APES, Sigma A3648). Before immunohistochemistry ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were permeabilized for one hour in a solution of phosphate-buffered saline (PBS) containing 3% bovine serum albumin (BSA, Sigma; A4503). Sections were incubated with primary antibodies in a solution of 1.5 % BSA and 0.2 % Triton X-100 overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were passaged and one of the following antibiotics were added to the medium in order to select for the cells which stably express the respective construct: Puromycin dihydrochloride (3 µg/ml, Sigma Aldrich), blasticidine S hydrochloride (15 µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transduced for 1-3 consecutive days with the viral stock diluted 1:3 with culture media in the presence (or absence for BT-549 cell line) of 8 µg/mL polybrene (Sigma-Aldrich). The viral solution was replaced with fresh culture medium ...
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bioRxiv - Biochemistry 2022Quote: K562 cell line derivatives were collected by centrifugation at 1400 x g for 3 mins and nuclei were isolated using the Nuclei EZ Prep nuclei isolation kit (Sigma- Aldrich). The nuclei were fixed in 4% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... seedlings were transferred for another 3 days in Hoagland containing poorly available Fe and 100 µM sodium orthovanadate or 150 µM glibenclamide (both from Sigma-Aldrich). For agar plates ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating VTA brain sections were incubated 1h at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% Bovine Serum Albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH ...
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bioRxiv - Plant Biology 2021Quote: ... Lipids extracts were incubated 1 h at 50°C in 2 mL of methylamine solution (7 ml methylamine 33% (w/v) in EtOH combined with 3 mL of methylamine 40% (w/v) in water (Sigma Aldrich) in order to hydrolyze phospholipids ...
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bioRxiv - Immunology 2021Quote: ... were seeded for 72 h on polycarbonate membrane Transwell permeable inserts (Costar, 3 μm pore size, 12 mm diameter, Sigma Ireland) and incubated for three days ...
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bioRxiv - Cell Biology 2021Quote: ... Dissociated testicular cells in PBS were fixed with PFA in 4% concentration for 5 minutes followed by centrifugation at 250 x g for 3 minutes to attach to coverslips coated with poly-D-lysine (Sigma Aldrich). Testicular cells were air-dried shortly ...