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Citations for Millipore Sigma :
6401 - 6450 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed 3 times by adding and removing 300 μl of wash buffer to each well (PBS + 0.05%Tween-20; Sigma, UK) and incubated for 1 hour with blocking buffer (Reagent Diluent ...
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bioRxiv - Cell Biology 2021Quote: ... were freshly oxidized by oxygen plasma and then incubated in a solution composed of 0.3% w/v 3- (trimethoxysilyl)propyl methacrylate (Sigma Aldrich) and toluene (Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were washed once with PBST and then incubated with the appropriate primary antibody diluted in PBST + 3% (v/v) BSA (Sigma) for 2 hours at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... embryos were cultivated at 28.5 °C for 3 days in fresh standard E3 medium supplemented with 0.05% methylene blue (Sigma Aldrich, MO) and 0.003% phenylthiourea (PTU ...
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bioRxiv - Microbiology 2021Quote: ... then washed and cultured one week in normal cell culture medium and passaged in 10 cm dishes seeded with 3×105 cells using culture medium supplemented with 30 μM 6-thioguanine (6-TG, Sigma). Cells were also plated without 6-TG to determine plating efficiency ...
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bioRxiv - Microbiology 2021Quote: ... Immunoreactive bands were detected by the addition of BCIP (5-bromo-4-chloro-3-indolylphosphate)-nitroblue tetrazolium solution (Sigma-Aldrich). The reaction was stopped after 2 min by washing the blots with large volumes of deionized water.
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bioRxiv - Neuroscience 2021Quote: ... The labeled samples were washed 3 times in PBT (10 min each) and incubated with DAPI (Sigma, cat. N. D9542) (5μg/ml ...
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bioRxiv - Immunology 2020Quote: Primary human NK cells were incubated after enrichment for 16 hours at 37°C in 3 ng/mL IL-15 (Miltenyi) in RPMI media supplemented with 10% Human AB serum (Sigma). Cells were washed with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... plasma was isolated from tail-blood and measured (3 μL in technical duplicates) using the Cholesterol Quantitation Kit (Sigma, MAK043) or the Triglyceride Quantification Colorimetric/Fluorometric Kit (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... were blocked for 1h in TBS containing 0.1% Tween-20 (TBS-T) supplemented by 3% fatty-acid free BSA (Sigma Aldrich) before incubation with the purified STARD10 protein (1 μg/mL in TBS-T + 3% BSA ...
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bioRxiv - Neuroscience 2021Quote: Astrocyte cultures were fixed with 4% PFA in PBS (pH 7.4) for 15 min and nonspecific sites were blocked with 3% bovine serum albumin (BSA; Sigma-Aldrich), 5% normal goat serum (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... larvae were anesthetized with 0.2 mg/mL ethyl-3-aminobenzoic acid ethyl ester (MESAB, Sigma-Aldrich E10521, St. Louis, MO) and mounted laterally in 2% low-melting temperature agarose (Thermo Fisher Scientific 16520) ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Immunology 2020Quote: Supernatants from epithelial cells were centrifuged at 5000g for 1 hr at 4 °C and the residual volume collected using 3 kDa molecular weight cut off 50 mL or 15 mL tube filters (Millipore). The lower volume was collected and volume measured then the residual volume from the upper level was diluted to a similar volume to the lower fraction ...
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bioRxiv - Neuroscience 2021Quote: Adult mosaic zebrafish (F0) were anesthetized in pH-buffered 0.2 mg/ml ethyl 3-aminobenzoate methanesulfonate solution (MS-222, Sigma-Aldrich). The caudal fin (2 mm of the end ...
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bioRxiv - Neuroscience 2021Quote: ... 5-DIV cultured human spinal motor neurons and C5-C7 ventral horns of adult animals 3 days after surgery were estimated using a Rac1/Cdc42 Activation Assay Kit (#17-441, Merck Millipore). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... Crystals occur over the course of 3 days in drops where the reservoir contained 100 mM Hepes pH 7.5 (Sigma-Aldrich), 1% v/v 2-methylpentanediol (MPD ...
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bioRxiv - Bioengineering 2021Quote: ... slides were either treated with SIGMA FAST™ BCIP/NBT (5-Bromo-4-chloro-3-indolulphosphate/Nitro blue tetrazolium, pH 9.5, Sigma) and counterstained with nuclear fast red (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... The phosphorylated peptides were released from IMAC beads by 3 × 100 μL 500 mM dibasic sodium phosphate (Na2HPO4, Sigma, S9763), pH 7.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... Beads were washed with TBS-lysis buffer and proteins were eluted from beads by addition of 3×Flag peptides (150 μg/mL, Sigma) for 1 h at 4°C and then subjected to tryptic digestion followed by LC-MS/MS ...
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bioRxiv - Neuroscience 2021Quote: ... the right hind paw of mice was inflamed by injection of 20 µl 3% (w/v) λ-carrageenan (Sigma-Aldrich), inflammation was induced while mice were under inhalation anesthesia (2% isoflurane) ...
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bioRxiv - Neuroscience 2021Quote: ... a DNA fragment encoding AviTag-GluN1(834-863) was synthesized by the Integrated DNA Technologies (Table 3) and inserted into pET-28a vector (Novagen) using NdeI and XhoI sites.
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bioRxiv - Biophysics 2021Quote: ... cells were treated for 10 min with a solution of 0.25% Triton-X100 in blocking buffer (solution of 3% bovine serum albumin (BSA, Sigma-Aldrich) in BRB80 buffer) ...
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bioRxiv - Cancer Biology 2021Quote: ... PC-3 cells were transfected with either the control or Sirt5 CRISPR/Cas9 plasmids according to the manufacturer’s instructions (Sigma-Aldrich). The transfection efficiency was determined by a green fluorescent protein (GFP ...
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bioRxiv - Neuroscience 2022Quote: ... the labeled SPs or anti-ACE2 antibody were purified using 3 kDa molecular weight cut-off ultrafiltration filter (Amicon Ultra Centrifugal Filter, Millipore). There was no detectable dye in the filtrate.
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bioRxiv - Microbiology 2022Quote: ... Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat. M7145) and sodium pyruvate (Gibco cat ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 30 minutes on a plate shaker at 37 °C with shaking, followed by addition of 3 μL of final 2.3 mM phenylmethylsulfonyl fluoride (PMSF, a serine hydrolase inhibitor) (Sigma Aldrich) diluted in PBS/isopropanol (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... A second cohort of SARS-CoV-2-infected male hamsters were intravenously co-injected with 18F-FES and 0.3 mg/kg of E2 (3% DMSO; Sigma-Aldrich) on day 7 post-infection (n = 4) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was spun at 1000g for 3 minutes and re-suspended in islet media-G11 mixed with concentrated lentivirus and DEAE-Dextran hydrochloride (Sigma Millipore). The mixture was loaded to the GravityPLUS hanging drop 96-well microplate (InSphero ...
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bioRxiv - Biochemistry 2022Quote: ... Loss of Memo was induced by 3 daily intraperitoneal injections with 2mg tamoxifen at age 4 weeks (T5648 Sigma-Aldrich, distributed through Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... The gelatin was subsequently cross-linked using N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC hydrochloride) (Sigma Aldrich Catalog No-03450) and N-Hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2020Quote: ... This solution was subsequently mixed with 2.5 μL of 280 mM OAc and 1 μL of positive control DNA or 50 nM negative control (CTX-M ESBL: 5’-ATTGACGTGC TTTTCCGCAA TCGGATTATA GTTAACAAGG TCAGATTTTT TGATCTCAAC TCGCTGATTT AACAGATTCG GTTCGCTTTC ACTTTTCTTC-3’; Sigma) to create the final RPA mix ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 μg proteins were separated by SDS-PAGE and electrotransferred onto Immobilon®-P transfer membranes (Merck Millipore, MA, USA), blocked with 5% skim milk in Tris-buffered saline with 5% Tween 20 (TBST) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Single colonies were grown overnight at 37 °C in 3 ml of LB (Hy-Labs) supplemented with 100 μg/ml ampicillin (Sigma). Plasmid DNA was purified using QIAprep spin miniprep kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Progeny were collected at eclosion and allowed to mate and develop for 3-5 days before being transferred to food mixed with 50ug/mL mifepristone (RU486, Sigma) or ethanol (control ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Neuroscience 2020Quote: ... The pellet was resuspended in HB and loaded onto a three-step Percoll gradient (3%, 10%, and 23%; Sigma-Aldrich) in HB without protease inhibitor cocktail ...
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bioRxiv - Neuroscience 2020Quote: ... was injected into the heart chamber of the fish along with a high molecular weight fluorescent dye (2000 kDa dextran-conjugated FITC (3 mg/ml, Sigma). We estimate that 1 nl of a 10 nM Aβ injection into a ∼3.01 (±0.16 ...
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bioRxiv - Molecular Biology 2020Quote: ... were cultured in the same medium conditions with the addition of the 2i chemical inhibitors: 3 μM of CHIR99021 and 250 μM of PD0325901 (Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... The RNAs were concentrated and buffer-exchanged into 10 mM NaHEPES pH 7.0 using Amicon® Ultra-15 centrifugal filter units with 3-kDa molecular weight cutoff (Millipore).
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bioRxiv - Microbiology 2020Quote: Cells were co-cultured with MA104 cells for 3 days in FBS-free medium supplemented with trypsin (0.5 μg/ml) (Sigma Aldrich) and lysed by freeze-thawing ...
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bioRxiv - Microbiology 2020Quote: ... primers 1022700 5F and R were used to amplify the 572 bp 5’ homology flank and primer pair 1022700 3F and R was used to amplify the 673 bp 3’ homology flank (KOD Hot Start DNA Polymerase, Merck Millipore) which were cloned on either side of the sfGFP expression cassette in pkiwi003 (Ashdown et al. ...
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bioRxiv - Microbiology 2020Quote: ... the bacterial suspension was discarded and tubes were gently washed 3 times with PBS and stained with 0.1% crystal violet (Sigma-Aldrich) for 15 min ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: Human endothelial cells or 3 days post-fertilization (dpf) zebrafish embryos with the yolk sac removed were lysed in RIPA buffer (Millipore) containing protease inhibitors (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were co-cultured for 3 days in FBS-free medium supplemented with porcine trypsin (0.5 μg/mL) (Sigma Aldrich). After incubation ...
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bioRxiv - Bioengineering 2021Quote: ... Constructs were then washed in PBS (3 x 5 min) and mounted to glass slides using Fluoroshield (Sigma Aldrich, F6182) mounting medium ...
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bioRxiv - Genomics 2021Quote: ... at room temperature with a thick layer of self-indicating silica gel (a granular material with c. 3 to 5mm irregular beads; e.g. Type III Sigma-Aldrich, S7625 ...
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bioRxiv - Bioengineering 2020Quote: Mature brain organoids at 1 to 3-months of age were incubated on polyethyleneimine (PEI) pre-treated MEA plates with 10 μg/mL laminin (Sigma) for 4 hours to ensure attachment to the plate ...