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Citations for Millipore Sigma :
6351 - 6400 of 10000+ citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... To remove residual boric acid the medium was treated overnight with B chelator Amberlite IRA-743 (3 g/L, Sigma). Before autoclaving ...
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bioRxiv - Neuroscience 2020Quote: ... The plates were later washed with sterile distilled water 3 times and incubated with 250 μl of 50 μg/ml fibronectin (Sigma) overnight at 4°C until further neuronal culturing.
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bioRxiv - Neuroscience 2020Quote: ... 5 ml of lysis buffer (10 mM Tris, 10 mM NaCl, 3 mM MgCl2, 0.1% Nonidet P40 substitute (Roche/ Sigma, 11754599001), 0.2 U μl−1 RNase inhibitor ...
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bioRxiv - Neuroscience 2020Quote: Mice were intraperitoneally injected with 3 doses of LPS dissolved in 0.1 mol phosphate balanced solution (PBS) (Escherichia coli O111: B4; Sigma-Aldrich) (50μg/kg bodyweight for each dose; ...
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bioRxiv - Physiology 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Microbiology 2021Quote: ... glucose were pelleted by centrifugation and resuspended in 5 mL YNBNAG11 pH 5.1 containing 5 μM 3-MB-PP1 (EMD MILLIPORE) or DMSO as a vehicle control ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were first washed 3×10min with PBS and then permeabilized with 0.1% Triton X-100 (T9284, Sigma-Aldrich) in PBS for 10 min at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... CTXφ production was induced in these strains by growing up to OD600=0.3 followed by 16 hour incubation at 37°C with aeration in LB supplemented with mitomycin C (20ng/mL) (Sigma). V ...
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Identification and biochemical characterization of a novel eukaryotic-like Ser/Thr kinase in E. colibioRxiv - Microbiology 2020Quote: ... coli DH5α cells were transformed with the resulting pho-lac fusion plasmids and streaked on dual indicator plates containing LB agar with 5-bromo-4-chloro-3-indolyl phosphate disodium salt (X-Phos) (Sigma) at a final concentration of 80μg/ml and 6-chloro-3-indolyl-β-D-galactoside (Red-Gal ...
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bioRxiv - Biochemistry 2021Quote: ... The purified proteins were analyzed by SDS-PAGE and MALDI-TOF and concentrated using ultra-filtration through a 3 kDa cut-off Amicon (Millipore) membrane ...
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bioRxiv - Cancer Biology 2020Quote: ... Percentage of GFP positive cell population was measured at day 3 or day 4 as initial time point using a Guava Easycyte HT instrument (Millipore). GFP% (mCherry% ...
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bioRxiv - Cell Biology 2021Quote: ... The reacted mixture was dialyzed in PBST buffer (DPBS with 0.05% v/v Tween 20) by a centrifugal filter with a 3 KDa molecular weight cut-off (Millipore UFC500324). The supernatant was immunoprecipitated by custom-made anti-RFP-beads (see Immunoprecipitation [IP] and Co-IP below) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by blocking buffer composed of PBS supplemented with 3% heat inactivated donkey serum (017-000-121, Jackson Immuno Research) and 0.1% Triton X-100 (T8787, Sigma-Aldrich). Primary antibodies were then diluted in blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cerebral cortices of three pups were dissected under sterile conditions and kept on ice in a sterile Falcon tube (15 mL) containing 3 mL of trypsin-EDTA 0.25% (Sigma-Aldrich) for 10 min ...
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bioRxiv - Biophysics 2020Quote: ... the base was suspended in PBS-glucose (3 ml) and 1.5 ml were gently transfer on a Percoll gradient (Percoll 60%-Percoll 30%, Sigma Aldrich) and centrifuged 20 min at 1700 rpm ...
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bioRxiv - Biophysics 2021Quote: All proteins were concentrated using ultrafiltration concentrators with either 3 kDa or 30 kDa MWCO regenerated cellulose membrane (EMD Millipore), flash frozen in small aliquots in liquid nitrogen ...
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bioRxiv - Biophysics 2020Quote: ... As oxygen scavenger a combination of 1 mM Trolox and the P2O system (3 units per ml pyranose oxidase, 90 units per ml catalase and 50 mM glucose, Sigma) were used.
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bioRxiv - Cancer Biology 2021Quote: ... cells were treated with 0.25μM testosterone 3-(O-carboxymethyl) oxime:BSA-fluorescein isothiocyanate (T-BSA-FITC) conjugate (T5771, Sigma-Aldrich, Munich, Germany) diluted in Tris-Buffer (pH 7.2 ...
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bioRxiv - Cancer Biology 2021Quote: ... A coated microplate was pre-warmed at 37°C and 3×105 cells were seeded in 675 μL of bicarbonate-free Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, D5030) supplemented with 1 mM pyruvate ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were plated at a density of ∼3 × 105 cells on 12-mm glass coverslips coated with 0.01% poly-L-lysine (Sigma, P4832). Half of the medium was changed every 3-4 days and experiments were performed at 12–13 days in vitro.
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bioRxiv - Cell Biology 2020Quote: ... 0.5 or 1 or 2 mM, Sigma, P4543; carbamazepine, 5 or 50 or 100 μM, Sigma, C4024; phenytoin, 3 or 30 or 100 μM, Sigma, D4007 ...
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bioRxiv - Cell Biology 2022Quote: ... and 3 mL volumes of culture were transferred per well to 6-well tissue culture-treated polystyrene plates (Sigma CLS3506) and incubated at 23°C for 48 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then blocked and permeabilized for 30 min at RT in blocking buffer that contained 3% bovine serum albumin (BSA) and 0.1% Triton X-100 (Sigma Aldrich). Subsequently ...
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bioRxiv - Cell Biology 2022Quote: ... Liposomes consisting of DPPC/egg yolk PC/egg yolk PG/cholesterol (6:3:1.2:0.95, DPPC from Avanti Polar Lipids, all other Sigma) with 0.6 μCi tracer [2-palmitoyl-1-14C]-dipalmitoyl phosphatidylcholine (14C-DPPC ...
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bioRxiv - Biochemistry 2022Quote: ... The refolded solution was left static over one day at 4 °C and concentrated with a compressed nitrogen-gas (or air) driven ultrafiltration device (350 ml Amicon Stirred Cell, 3 kDa NMWL membrane, Millipore), during which time cysteine in the solution gradually react to form cystine and precipitated out and could usually clog the ultrafiltration membrane at the end phase of ultrafiltration ...
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bioRxiv - Biochemistry 2022Quote: ... were combined and concentrated using an Amicon concentrator tube (30 kDa MWCO for the Nb- fused biosensors, 3 kDa MWCO for HER2-Nb) (Merck-Millipore). A final volume < 5 mL was loaded onto a SEC column (HiLoad 200pg ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids following a similar protocol and transgenic parasites were obtained after 3 weeks of selection with 100 μg/ml G418 and 200 μg/ml Hygromycin B (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... HD-MBO3 group 3 MB cells were generously provided by Till Milde (DKFZ, Germany) and cultured in RPMI medium (Sigma). Human embryonic kidney HEK-293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2022Quote: ... Fractions containing pure Pae87 were desalted and concentrated with Amicon Ultra centrifugal filters of 3 kDa or 10 kDa MWCO (Millipore). Concentrated samples were centrifuged at 15,000 × g for 10 min prior to crystallization trials ...
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Physiology 2022Quote: ... were mixed with 3 µl proteinase K (20 mg/ml in 25 mM Tris-HCl, 5 mM Ca2Cl, pH 8.0, Sigma-Aldrich). This mixture was applied to the ear clips ...
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bioRxiv - Plant Biology 2022Quote: ... and sterilized for 3 h in a mixture of bleach (30 mL) and concentrated hydrochloric acid (1.5 mL, Sigma-Aldrich). The pgb1-1 mutant lacks ethylene-induced PGB1 expression and lower hypoxia induced PGB1 expression (relative to wild-type ...
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bioRxiv - Pathology 2022Quote: Lung tissue (4–20 g) was inflated with warm low temperature gelling agarose (3% by weight in DMEM w/o Phenol red, A9414, Sigma) at room temperature using 1-mL syringe ...
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bioRxiv - Microbiology 2022Quote: ... DSF (CAS No. 677354-23-3, purity >90.0%) and BDSF (CAS No. 55928-65-9, purity >95.0%) purchased from Sigma Aldrich (USA) and trans-BDSF (CAS No ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Developmental Biology 2022Quote: ... permeabilized for 30 min at room temperature in DPBS/0.3% Triton-X100 and block by blocking buffer (PBST containing 3% donkey serum (Sigma, D9663) and 1% BSA (Sigma ...
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bioRxiv - Genomics 2022Quote: ... the mice were anesthetized again with 3% isoflurane and immediately administered 100 μl of 15 mM AkaLumine-HCL (Sigma Aldrich) for imaging ...
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bioRxiv - Microbiology 2022Quote: Mice were fasted for 4hrs before oral gavage with 200uL of fluorescein isothiocyanate (FITC)-dextran (3-5 kDa, Sigma-Aldrich) dissolved in sterile PBS (60mg/ml) ...
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bioRxiv - Cell Biology 2022Quote: ... mycelia from 3-day old liquid cultures were harvested by straining the culture through Miracloth (Millipore Sigma catalog number 475855). The mycelia were transferred to a 50 ml Falcon tube and washed twice in 0.7 M NaCl by pelleting mycelia at 8,000 rpm and 25°C for 5 minutes using a JA25.50 rotor and resuspending in sterile saline solution ...
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bioRxiv - Cell Biology 2022Quote: ... Cell supernatant containing the virus was collected 3 days after transfection and 20-fold concentrated using Amicon Ultra 15 10K (Millipore) centrifugal filter ...
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bioRxiv - Bioengineering 2022Quote: ... All primary cells were used between subculture 3–7 and were routinely cryopreserved in 90% human serum (#H4522; Sigma-Aldrich) supplemented with 10% dimethyl sulfoxide (DMSO).
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated for 3 days in the presence of 100 μg/ml heparin from porcine intestinal mucosa (Sigma, H3393) to prevent LRP1-mediated endocytic clearance before harvesting ...
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bioRxiv - Biophysics 2022Quote: ... The recordings for PI(3, 5)P2 injection were performed under ND-96 solution containing 10 µM Ani9 (Sigma-Aldrich) to inhibit the endogenous Ca2+- activated Cl- channel ...
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bioRxiv - Biophysics 2022Quote: ... A negative control without tyrosinated tubulin was prepared by treating 3 mg/mL bovine tubulin with 0.02 mg/mL carboxypeptidase A (CPA, Sigma Aldrich) on ice for 15 min to proteolytically cleaved the C-terminal tyrosine of α-tubulin ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with CK+DCIR additives (3 μM CHIR99021 [CHIR; PeproTech], 10 ng/mL keratinocyte growth factor [KGF; PeproTech], 50 nM dexamethasone [Sigma] ...
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bioRxiv - Biochemistry 2022Quote: ... The column was washed with 30 column volumes (CV) of wash buffer before bound proteins were eluted with wash buffer supplemented with 3 mM D-desthiobiotin (Sigma). The protein was transferred into wash buffer without D-desthiobiotin and incubated overnight at 4°C with 1:100 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... The electrode was then immersed in 3 mL of a mixed buffer solution containing 5 mM of HEPES (Sigma Aldrich), 5 mM of MES (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3% Triton X-100 in PBS) and with primary antibodies overnight at +4 °C (primary Abs: mouse anti-flag –Sigma F1804 ...
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bioRxiv - Genetics 2022Quote: ... Degradation of the AID-tagged RAD21 was induced with addition of 500 μM indole-3-acetic acid (auxin; Sigma-Aldrich). Degradation of AID-tagged WAPL was induced with addition of 1μM 5-Ph-IAA (modified auxin ...
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bioRxiv - Cell Biology 2022Quote: The tissue section on the Stereo-seq chip (5 cm x 3 cm or 2 cm x 3 cm) was then incubated at 37°C for 5-8 min and subsequently fixed in methanol (Sigma, 34860 ...