Labshake search
Citations for Addgene :
4701 - 4750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... The 4xSID sequence was derived from Addgene 1063994 and the 3xKRAB sequence was taken from Addgene 12813244 ...
-
bioRxiv - Genetics 2024Quote: ... and incorporated into the SfiI digested pMPRAv3:Δluc:ΔxbaI (Addgene #109035) backbone vector by Gibson assembly (50uL 2xNEB HiFi Assembly MM ...
-
bioRxiv - Immunology 2024Quote: ... annealed and individually cloned into vector plasmids lentiGuide-Puro (Addgene #52963) or lentiGuide-mCherry (Plasmid #170510) ...
-
bioRxiv - Immunology 2024Quote: ... and 30μg packaging plasmid psPAX2 (A gift from Didier Trono (Addgene #12260)) in opti-MEM media (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... pMD2.G was a gift from Didier Trono (Addgene plasmid #12259); psPAX2 was a gift from Didier Trono (Addgene plasmid #12260) ...
-
bioRxiv - Cancer Biology 2024Quote: ... psPAX2 was a gift from Didier Trono (Addgene plasmid #12260). Single-guide RNAs (sgRNAs ...
-
bioRxiv - Immunology 2024Quote: ... and pMD2.g (Addgene cat#12259) using Lipofectamine 3000 (ThermoFisher cat# L3000001 ...
-
bioRxiv - Immunology 2024Quote: All constructs were cotransfected into HEK293T cells with lentiviral packaging vectors psPAX (Addgene cat#12260) and pMD2.g (Addgene cat#12259 ...
-
bioRxiv - Immunology 2024Quote: ... each RBD variant was tagged with a unique 26-nucleotide (N26) barcode via PCR and assembled into Yeast surface display vector (Addgene, 166782). The XBB.1.5 and JN.1 DMS libraries were further transfected into electrocompetent DH10B cells for plasmid amplification and proceed to PacBio sequencing library preparation to decipher the association between RBD variant and corresponding N26 barcode ...
-
bioRxiv - Genetics 2024Quote: ... All the sgRNAs were expressed using the U6- promoter and subcloned into the PX458 vector (Addgene ID: 112220) to construct the PX458-sgRNAs targeting vector ...
-
bioRxiv - Genetics 2024Quote: ... The sgRNA was in vitro transcribed from a SP6 transcription template amplified from pDD162 (Addgene #47549) using primers P50 and P40 ...
-
bioRxiv - Genomics 2024Quote: ... and tdTomato (sequence obtained from pCSCMV: tdTomato Addgene #50530). For gene annotation ...
-
bioRxiv - Genetics 2024Quote: ... the two top scoring sgRNAs were cloned into BsmBI-digested lentiGuide-Puro vector (Addgene #52963) (Table S3) ...
-
bioRxiv - Neuroscience 2024Quote: ... EGFP-ESyt2 (#66831) and GFP-C1-PLCdelta-PH (#21179) were obtained from Addgene. The following constructs were kindly provided ...
-
bioRxiv - Neuroscience 2024Quote: ... Plcxd2fl/fl:Rorb-Cre and Plcxd2+/+:Rorb- Cre pups were injected with 50nL of a mixture containing AAV-TRE-DIO-FLPo (Addgene #118027) and AAV-TRE-fDIO-GFP-IRES-tTA (Addgene #118026 ...
-
bioRxiv - Immunology 2024Quote: pcDNA3.1 plasmids encoding the C9-tagged SARS-CoV-2 S protein (pcDNA3.1-SARS2-S) (Addgene plasmid # 145032)62 ...
-
bioRxiv - Genomics 2024Quote: ... pBWB514 (Addgene #209326). The additional restriction sites make possible the transfer of mapped libraries ...
-
bioRxiv - Immunology 2024Quote: ... TRE CAR construct was generated from pHR_Gal4UAS_pGK_mCherry (Addgene #79124) by inserting a TRE promoter and removing GAL4 UAS promoter via EcoRI restriction digestion and cloning CD19 CAR construct into the multiple cloning site ...
-
bioRxiv - Immunology 2024Quote: iOnEmpty vector was built into the TLCV2 plasmid from Addgene (#87360) by amplifying and cloning its own tight TRE promoter into the backbone and removing the U6 Promoter-empty gRNA-Tight TRE Promoter-Cas9 cassette via KpnI-BamHI restriction digestion ...
-
bioRxiv - Genomics 2024Quote: ... a variant of which containing a ribosomal binding site and mRFP in place of a gene fragment has been deposited to Addgene (Addgene #209325). A slight modification to this plasmid was made to include SapI type II restriction sites ...
-
bioRxiv - Immunology 2024Quote: ... and subsequently the iOffCAR constructs were built on iOnEmpty by swapping the rtTA with tTA from the pHR_PGK_antiCD19_synNotch_TetRVP64 vector from Addgene (#79126) via XbaI-XhoI sites ...
-
bioRxiv - Immunology 2024Quote: ... Anti-CD19 scFv amino acid sequence was obtained from Addgene plasmid #79125 ...
-
bioRxiv - Molecular Biology 2024Quote: ... a sgRNA-resistant version of N6AMT1-3xFLAG cDNA was synthesized in pWPI (Addgene). The same cDNA was further edited to obtain the D77A variant using site-directed mutagenesis ...
-
bioRxiv - Molecular Biology 2024Quote: ... LOX-1 tagged with V5-6×His at the C-terminus (V5-LOX-1) was subcloned into pmScarlet_C1 (plasmid #85042; Addgene) (mScarlet-LOX-1) ...
-
bioRxiv - Neuroscience 2024Quote: ... and 750 nl of AAV2-hSyn-DIO-mCherry (50459-AAV2, Addgene) into the left PrL cortex (from bregma ...
-
bioRxiv - Neuroscience 2024Quote: ... 500 nl of AAV5-hSyn-hChR2(H134R)-EYFP (26973-AAV5, Addgene, Watertown, MA) into the left BLA (from bregma ...
-
bioRxiv - Genomics 2024Quote: The plasmid backbone pBbA2c-RFP (Addgene #35326) (Lee et al ...
-
bioRxiv - Genomics 2024Quote: ... 2.5µg of PMD2.G (Addgene; 12259) and 2.5µg psPAX2 (addgene;12260 ...
-
bioRxiv - Genomics 2024Quote: ... which was a gift from Alexander Stark (Addgene plasmid # 99296 ...
-
bioRxiv - Genomics 2024Quote: ... and pMD2.G (psPAX2 and pMD2.G were a gift from Didier Trono, Addgene plasmid # 12260 ...
-
bioRxiv - Genomics 2024Quote: ... 0.825 μg psPAX2 (Addgene 12260) and 0.55 μg pMD2.G (Addgene 12259) ...
-
bioRxiv - Genomics 2024Quote: ... we exchanged the PuroR cassette in pLentiRNAGuide_001 (Addgene 138150) with a GFP-P2A-Puro cassette from pLentiRNAGuide_003 (Addgene 192505 ...
-
bioRxiv - Genomics 2024Quote: ... were obtained from Addgene. GUS control plasmid was provided as part of the LR Clonase II enzyme kit (Invitrogen 11791020) ...
-
bioRxiv - Genomics 2024Quote: Lentivirus was produced via transfection of pooled library plasmids with appropriate packaging plasmids (psPAX2-Addgene 12260; pMD2.G-Addgene 12259) using linear polyethylenimine (PEI ...
-
ZNF143 binds DNA and stimulates transcription initiation to activate and repress direct target genesbioRxiv - Genomics 2024Quote: ... We cleaved the hSpCas9 plasmid PX458 (Addgene #48138) with the enzyme BbsI ...
-
bioRxiv - Genomics 2024Quote: ... with a GFP-P2A-Puro cassette from pLentiRNAGuide_003 (Addgene 192505) using NheI and ApaI restriction sites ...
-
bioRxiv - Genomics 2024Quote: ... pCW57.1 (pCW57.1 was a gift from David Root, Addgene plasmid # 41393 ; http://n2t.net/addgene:41393 ; RRID:Addgene_41393), psPAX2 ...
-
bioRxiv - Genomics 2024Quote: ... pCW57.1 (pCW57.1 was a gift from David Root, Addgene plasmid # 41393 ...
-
bioRxiv - Genomics 2024Quote: Lentivirus was produced via transfection of pooled library plasmids with appropriate packaging plasmids (psPAX2-Addgene 12260; pMD2.G-Addgene 12259) using linear polyethylenimine (PEI ...
-
bioRxiv - Genomics 2024Quote: ... and 0.55 μg pMD2.G (Addgene 12259). Two days post-transfection ...
-
bioRxiv - Genomics 2024Quote: MORF library without GFP and mCherry controls (Addgene #192821) was transformed into electrocompetent NEB Stable E ...
-
bioRxiv - Genomics 2024Quote: ... lacZ reporter gene and H11 locus homology arms (Addgene, 139098) using NEBuilder HiFi DNA Assembly Mix (NEB ...
-
bioRxiv - Genomics 2024Quote: ... Gramberg) together either with the plasmid pAPM-D4-miR30-L1221 (a gift from Jeremy Luban; Addgene #115846) (27 ...
-
bioRxiv - Microbiology 2024Quote: ... the LC3B in pBABE-puro mCherry-EGFP-LC3B (Addgene, Plasmid #22418) was replaced with the LC3C at EcoRI and SalI site to make pBABE-puro mCherry-EGFP-LC3C ...
-
bioRxiv - Genomics 2024Quote: ... and pGal/Pol (Addgene, Watertown, MA). The transfected cells were incubated for 16 h ...
-
bioRxiv - Genomics 2024Quote: ... expressing a control shRNA or plasmid pAPM-D4 miR30-TRIM28 ts3 (Addgene #115864) (27 ...
-
bioRxiv - Genomics 2024Quote: ... The DUX4-inducible plasmid pCW57.1-DUX4-CA was obtained from Addgene (#99281).
-
bioRxiv - Microbiology 2024Quote: The assembled tRNA genomic sequence was then inserted into the EcoRI/PstI-digested pBSTNAV vector (AddGene, USA). The final construct contained the tRNA under the control of the constitutive lpp promoter ...
-
bioRxiv - Immunology 2024Quote: ... 10μg envelope plasmid pMD2.G (A gift from Didier Trono (Addgene #12259)) and 30μg packaging plasmid psPAX2 (A gift from Didier Trono (Addgene #12260) ...
-
bioRxiv - Genetics 2024Quote: ... Amplicons were cloned into a plasmid for targeting the AAVS1 safe harbor locus (AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343 ...