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Citations for Addgene :
4751 - 4800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... EGFP-RAB6A (#49469) and LAMP1-mGFP (#34831) were purchased from Addgene. pcDNA3.1+-mCherry was kindly provided by Dr ...
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bioRxiv - Cell Biology 2024Quote: ... Carol Otey (UNC-Chapel Hill) and pTriEx/mCherry-PA-Rac1 (Addgene #22024) was obtained from Dr ...
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bioRxiv - Cell Biology 2024Quote: ... Both sgRNAs were cloned into an all-in-one vector encoding Cas9 D10A nickase (Addgene) with puromycin as a selection marker ...
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bioRxiv - Cell Biology 2024Quote: ... The TRPML1-GCamP6s expression construct was created by subcloning the human TRPML1 insert from the human TRPML1-EYFP plasmid 63 into the pGP backbone harboring GCaMP6s (Addgene #40753) using restriction sites BglII (N-terminal ...
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bioRxiv - Cell Biology 2024Quote: ... the GCaMP8s was amplified from pGP-CMV-jGCaMP8s (Addgene #162371) and inserted into pLentiCMVie-lyso-BlastR using the restriction sites EcoRV and BamHI ...
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bioRxiv - Cell Biology 2024Quote: ... The TMEM165-mCherry expression construct was generated by inserting mCherry into pLentiCMVie-IRES-BlastR (Addgene #119863) using the restriction sites XhoI and BamHI ...
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bioRxiv - Cell Biology 2024Quote: ... VAP-A (104447) and VAP-B (104448) in pEGFP-C1 and mEmerald-tagged Sec61β in pEGFP-C1 (90992) were obtained from Addgene. VAP-A(8∼212 ...
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bioRxiv - Developmental Biology 2024Quote: ... and Cry2 and Cib1N coding sequences were obtained from Addgene (accession numbers 26866 and 26867 ...
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bioRxiv - Cell Biology 2024Quote: ... The pLKO.1 vector (Addgene, 10878) was digested with AgeI-HF (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng p-CMV-VSV-G (Addgene, 8454), 900 ng psPax2 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... The FAP-tagging plasmids and those expressing these FAP-tagged cellular markers are all available on Addgene (see Supplemental Table 2 or Addgene global deposit number 84326). Plasmids were transformed into yeast cells using the lithium acetate method (Ausubel ...
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bioRxiv - Cell Biology 2024Quote: The cDNAs for H3.1-SNAP-3XHA and H3.3-SNAP-3XHA were cloned sequentially into pMSCV-puro retroviral vectors obtained from Addgene (Plasmid 68469). Briefly ...
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bioRxiv - Cell Biology 2024Quote: The construct mCherry2-C1 was a gift from Michael Davidson (Addgene plasmid # 54563 ...
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bioRxiv - Cell Biology 2024Quote: ... all above constructs were purchased from Addgene. The pcDNA3-OMM-AKAR4 sensor and pcDNA3-PKA-mCherry were previously generated in our laboratory(11) ...
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bioRxiv - Cell Biology 2024Quote: ... the CAAX domain of K-ras tagged with mTagRFP-T was introduced into a safe harbor locus via CRISPR using a donor plasmid developed at the Allen Institute for Cell Science and sourced from Addgene (Plasmid #107580a). We call the resulting cell line CT20 ...
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bioRxiv - Cell Biology 2024Quote: ... DREADD-hM4Di was obtained from Addgene (plasmid # 45548).
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bioRxiv - Cell Biology 2024Quote: ... 200 ng linearized pLHCX-XBP1 mNeonGreen plasmid NLS (Addgene plasmid #115971 ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... monolayers of HCT116 cells incubated at 37 °C and under 5% CO2 were transfected with RedTrackCMV (vector control; #50957, Addgene) or RedTrackCMV-LC3B (RedTrackCMV containing the gene encoding LC3B ...
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bioRxiv - Cell Biology 2024Quote: ... 200 ng linearized pLHCX-XBP1 mNeonGreen plasmid NLS (Addgene plasmid #115971; http://n2t.net/addgene:115971; RRID:Addgene_115971), a gift from David Andrews (Nougarède et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The Lyso-GCaMP8s expression construct was generated by inserting Lyso into pLentiCMVie-IRES-BlastR (Addgene #119863) via the EcoRI and EcoRV sites ...
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bioRxiv - Developmental Biology 2024Quote: ... These constructs were then cloned into PCR4-Shh:LacZ-H11 plasmids (Addgene plasmids, #139098), placing them upstream of a minimal Shh promoter and LacZ reporter gene ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... PB-Ef1a-PCP-Halo (Addgene plasmid # 198337) and PB-Ef1a-β-actin-UTR-PP7 mRNA were gifts from Michael Ward.
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... Rat LAMP1 was amplified from LAMP1-RFP (Addgene plasmid # 1817) with the known D50E mutation corrected by PCR ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... pAAV hSyn GFP-Fxr1 was a gift from Martin Beaulieu (Addgene plasmid # 112732)60 ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... mNeonGreen was amplified from H2B-mNeonGreen-IRESpuro2 (Addgene plasmid # 183745) flanked by an EcoRI site ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... pLKO.1 puro was a gift from Bob Weinberg (Addgene plasmid # 8453)54 ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... mito-V5-APEX2 was a gift from Alice Ting (Addgene plasmid # 72480)59 ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... same construction procedure was performed but omitting APEX2 during amplification from mito-V5-APEX2 (Addgene plasmid # 72480).
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... V5-APEX2 was amplified from mito-V5-APEX2 (Addgene plasmid # 72480). LAMP1 was amplified from RUSH-LAMP1-mScarleti after the 20 a.a ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... mScarleti was amplified from pmScarlet-i_C1 (Addgene plasmid # 85044) with an EcoRI site introduced at the 3’ end ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... pmScarlet-i_C1 was a gift from Dorus Gadella (Addgene plasmid # 85044)55 ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... Strep-KDEL-intron-IRES was amplified from Str-KDEL_SBP-EGFP-Ecadherin (Addgene plasmid # 65286) with XbaI and EcoRI sites introduced and inserted into restricted FUGW by GIBSON assembly ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... psPAX2 and pMD2.G were gifts from Didier Trono (Addgene plasmids # 12260 and # 12259) pmScarlet-i_C1 was a gift from Dorus Gadella (Addgene plasmid # 85044)55 ...
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bioRxiv - Cell Biology 2024Quote: ... pLex306 plasmid (David Root, Addgene plasmid #41391) containing the open reading frames of either was co-transfected with psPAX2 and pMD2.G in HEK293FT cells at 50% confluency in a 10 cm dish using lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... a fragment containing mTurquoise2 and the mSTING CDN was amplified by PCR and cloned in a pLex306 backbone (David Root, Addgene plasmid #41391). The sequence of E ...
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bioRxiv - Cell Biology 2024Quote: ... pLex306 plasmid (David Root, Addgene plasmid #41391) containing the open reading frames of either sequence was co-transfected with psPAX2 and pMD2.G in HEK293FT cells at 50% confluency in a T75 flask using lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: YFP NLS Beta-Actin G13R (Addgene no. 60615) was used to construct the mEos3.2 actin-G13R plasmid using SalI-HF and BamH1 to combine the mutated region of actin with existing mEos3.2 actin plasmids ...
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bioRxiv - Cell Biology 2024Quote: mEos 2 Paxillin-22 was a gift from Michael Davidson (Addgene no, 57409).
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bioRxiv - Cell Biology 2024Quote: mEmerald-LC-Myosin-N7 was a gift from Michael Davidson (Addgene no. 54146). mNeon LC-Myosin-N7 was constructed using the enzymatic digests described in the Supplementary Information.
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bioRxiv - Cell Biology 2024Quote: ... Short hairpin RNAs (shRNAs) targeting WSB2 or BCL-2 family proteins were subcloned into the pLKO.1 puro vector (Addgene) for gene KD ...
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bioRxiv - Cell Biology 2024Quote: CRISPR CAS9 mediated knockout of CD44 was performed in HT1080 cells by designing sgRNAs against CD44 exon using the online available software (https://chopchop.cbu.uib.no/) and cloning into pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene Plasmid# 62988, gift from Feng Zhang) according to the protocol described elsewhere30 ...
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bioRxiv - Cell Biology 2024Quote: ... and pMD2.G (Addgene, #12259) in a ratio of 3:2:1 ...
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bioRxiv - Cell Biology 2024Quote: ... BFP-KDEL was a gift from Gia Voeltz (Addgene plasmid # 49150) (Friedman et al. ...
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bioRxiv - Cell Biology 2024Quote: ... a gift from Matt Welch (Addgene no. 8462)46 ...
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bioRxiv - Cell Biology 2024Quote: pENTR-NLS-actin-R62D (Addgene no. 11831) was used to construct the mEos3.2 actin-R62 plasmids using Sali-HF and BmbG1 to combine the mutated region of actin with existing mEos3.2 actin plasmids ...
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bioRxiv - Cell Biology 2024Quote: ... pTG023 was constructed using PCR amplification of Plas-gRNA-LEU (Addgene #309094), a gBlock designed to fuse csGBP-mAID (IDT ...
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bioRxiv - Cell Biology 2024Quote: ... and of pRSII402 (Addgene #35434). Fragments were purified before mixing with HiFi Assembly Mastermix (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... mEmerald-Sec61β-C1 was a gift from Jennifer Lippincott-Schwartz (Nixon-Abell et al., 2016; Addgene plasmid #90992). The following eGFP-Rab plasmids were gifts from Marci Scidmore ...
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bioRxiv - Cell Biology 2024Quote: ... eGFP-Rab7a was a gift from Qing Zhong (Sun et al., 2010; Addgene plasmid #28047). pmScarlet_alphaTubulin_C1 was a gift from Daphne Bindels (Bindels et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The following eGFP-Rab plasmids were gifts from Marci Scidmore: eGFP-Rab6a (Rzomp et al., 2003; Addgene plasmid #49469), eGFP-Rab6a Q72L (Moorhead et al. ...