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Citations for Addgene :
4801 - 4850 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Enzymatically dead Cas9-KRAB fusion protein (Lenti-dCas9-KRAB-blast, Addgene, #89567) was introduced into three KP LUAD cell lines by lentiviral transduction and blasticidin selection ...
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bioRxiv - Cancer Biology 2024Quote: HEK293T cells were transfected with a shuttle vector plasmid and packaging plasmids pMD2.G (Addgene, #12259) and psPAX (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... All sgRNAs used for validation (Supplementary Table 7) were cloned into the pLenti-sgRNA(lib)-puro vector (Addgene, #119976) through Golden Gate assembly ...
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bioRxiv - Cancer Biology 2024Quote: pLenti-ABEmax-P2A-EGFP expression plasmid was constructed by cloning ABEmax_P2A_EGFP sequence from pCMV_ABEmax_P2A_GFP (Addgene, #112101) into the lentiviral vector ...
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bioRxiv - Developmental Biology 2024Quote: ... The Sp-Cas9 plasmid (Addgene plasmid #62731) was utilized to synthesize SpCas9 as described previously (36) ...
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bioRxiv - Microbiology 2024Quote: ... pLenti PGK GFP Puro (w509-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19070 ...
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bioRxiv - Microbiology 2024Quote: ... psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260 ...
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bioRxiv - Microbiology 2024Quote: ... pCDH-PGK was a gift from Kazuhiro Oka (Addgene plasmid # 72268 ; http://n2t.net/addgene:72268 ; RRID:Addgene_72268). Supernatant was harvested at 40- and 64-h post-transfection and filtered using a 45μM PES filter ...
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bioRxiv - Microbiology 2024Quote: ... and pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ...
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bioRxiv - Microbiology 2024Quote: ... pLenti PGK GFP Puro (w509-5) was a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 19070 ; http://n2t.net/addgene:19070 ; RRID:Addgene_19070). The PGK-UL12.5-SPA plasmid was generated by cloning UL12.5-SPA from pLenti CMV Neo UL12.5-SPA 29 ...
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bioRxiv - Microbiology 2024Quote: ... and pCMV-VSV-G was a gift from Bob Weinberg (Addgene plasmid # 8454 ; http://n2t.net/addgene:8454 ; RRID:Addgene_8454). The same approach was used to generate lentiviruses expressing UL12.5-SPA (PGK-UL2.5-SPA) ...
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bioRxiv - Cancer Biology 2024Quote: ... the packaging plasmid psPAX2 (Addgene: 12260), and pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transiently transfect with BFP-KDEL (Addgene, 49150) and imaged 48 hr later ...
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bioRxiv - Cell Biology 2024Quote: ... and safe-targets (sgSAFE #5784) (see Table 1) were selected from the Bassik Human CRISPR Knockout Library (Addgene, 101926 ...
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bioRxiv - Cancer Biology 2024Quote: ... and annealed and ligated in the pLKO.1 Hygro linearized backbone (a gift from Bob Weinberg, plasmid #24150 on Addgene). Stbl3 bacteria were heat-shocked to uptake ligated plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Three sgRNAs targeting each candidate enhancer were cloned into LentiGuide-Puro backbone (LentiGuide-Puro, Addgene, #52963). A non-targeting sgRNA and sgRNAs that target the Nupr1 gene body were used as negative and positive controls ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: Cells were seeded in 12-well plates and transfected at 50-70% confluence with 0.5 µg/well TOPflash (Addgene, #12456) and 0.3 µg pRL-TK (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... in which the TURBO-ID region was substituted by a 3HA-tag and the murine form of Sox11 (obtained from Addgene: #120387) was cloned after the tag (between restriction sites for EcoRI and BamHI) ...
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bioRxiv - Cancer Biology 2024Quote: ... James D Sutherland (Addgene: #208041) in which the TURBO-ID region was substituted by a 3HA-tag and the murine form of Sox11 (obtained from Addgene ...
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bioRxiv - Cell Biology 2024Quote: Peredox NADH/NAD+ sensor (Cat# 163060, Addgene) was transfected into HEK293 cells for lentivirus production ...
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bioRxiv - Cell Biology 2024Quote: ... previously described in (Raz, Abraham et al. 2011) were cloned into the pCW57-MCS1-2A-MCS2 doxycycline (Dox) inducible lentiviral vector (Addgene plasmid #71782). Cloning was confirmed by sanger sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... pUltra-EGFP-FL-NRL was produced by amplification of the FL-NRL coding sequence from pCDNA4-C-EF1α-FL-NRL and inserting into BamHI digested pUltra-EGFP (Addgene 24129) (primers #12 ...
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bioRxiv - Cell Biology 2024Quote: Plasmid containing full length YAP1 with T2A-mCherry tag (#74942, Addgene) was transfected together with envelope expressing plasmid 9 - PMS2.G (#12259 ...
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bioRxiv - Cell Biology 2024Quote: ... and empty backbone packaging plasmid PSPAX2 (#12260, Addgene) into HEK293T cells using FuGENE® HD Transfection Reagent according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... immortalized parental fibroblasts (JR20) (37) were transfected with pSpCas9(BB)-2A-Puro (PX459) V2.0 (Plasmid 62988, Addgene) with target sequence 5’-AAACTTCATCAATAACCCGC-3’ generated using established protocols (38) ...
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bioRxiv - Cell Biology 2024Quote: ... lenti MS2-P65-HSF1_Hygro (Addgene, #61426) and dCAS9-VP64_GFP (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... OPTN217-577-GFP (Addgene, plasmid #68841 [41]), or OPTND474N-GFP (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... OPTN1-577-GFP (Addgene, plasmid 27052 [54]), OPTN1-209-GFP (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... SgRNAs were cloned into lenti sgRNA (MS2)_zeo backbone vector (Addgene, #61427). For CRISPRi assays ...
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bioRxiv - Cell Biology 2024Quote: ... Tom Rapoport (Addgene plasmid 86683)30 mCh Climp-63 and mCh sec61β was a kind gift from Prof ...
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bioRxiv - Cell Biology 2024Quote: ... the following cDNA sequences were cloned into pLKO.1 which was a gift from David Root (Addgene plasmid # 10878). by Genscript Corporation:
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bioRxiv - Cell Biology 2024Quote: ... and marker pCFJ90 (Pmyo-2-mCherry; Addgene #19327, 2.5 ng/µL) was injected into N2 young adult hermaphrodites ...
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bioRxiv - Cell Biology 2024Quote: ... The sgRNA 5’-CGTTTTCAGAGTGATGGCGA-3’ targeting the efa-6 ATG was selected using CRISPR design tool (http://crispr.mit.edu) and was inserted into pDD162 vector (Addgene #47549) using primers YJ12362 and YJ12363 (Table S2/3 ...
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bioRxiv - Cell Biology 2024Quote: ... and cloned into pDD282 (Addgene #66823, FP::SEC vector) by Gibson assembly (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... co-injection marker pCFJ104 (Pmyo-3-mCherry; Addgene #19328, 5 ng/µL) and marker pCFJ90 (Pmyo-2-mCherry ...
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bioRxiv - Cell Biology 2024Quote: ... was transfected together with envelope expressing plasmid 9 - PMS2.G (#12259, Addgene), and empty backbone packaging plasmid PSPAX2 (#12260 ...
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bioRxiv - Cell Biology 2024Quote: TurboID sequence was amplified from a pcDNA3 plasmid containing 3xHA-TurboID-NLS (Addgene #107171). Flag tag sequence was added to the N-terminus using a forward primer ...
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bioRxiv - Cell Biology 2024Quote: ... two packaging plasmids (pMDLg/pRRE, Addgene #12251 and pRSV-Rev, Addgene #12253) and the envelope expressing plasmid (pCMV-VSV-G ...
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bioRxiv - Cell Biology 2024Quote: ... two packaging plasmids (pMDLg/pRRE, Addgene #12251 and pRSV-Rev, Addgene #12253) and the envelope expressing plasmid (pCMV-VSV-G ...
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bioRxiv - Cell Biology 2024Quote: The constructs for SF-Ch2 assays were obtain from Addgene (plasmid #82604 and #82602). Constructs were then cloned and expressed using the lentiviral system as described above ...
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bioRxiv - Cell Biology 2024Quote: The monoclonal SK-N-DZ SCLYKO cell line was transduced with Toronto KnockOut (TKO) CRISPR Library - Version 3 (Addgene, 90294) at MOI of 0.3 ...
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bioRxiv - Cell Biology 2024Quote: Individual sgRNAs were cloned into the pLenti-CRISPR-V2 (Addgene #98293) lentivectors for CRISPR Knock-out (CRISPR-KO ...
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bioRxiv - Cell Biology 2024Quote: ... and the envelope expressing plasmid (pCMV-VSV-G, Addgene #8454). Virial supernatant was harvested 48 h post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... and dCAS9-VP64_GFP (Addgene, #61422) were used ...
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bioRxiv - Cell Biology 2024Quote: ... Oligonucleotides for shRNAs were cloned into pLKO.1 vector (Addgene, #21297). The day before transfection ...
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bioRxiv - Cell Biology 2024Quote: ... pHR-SFFV-dCas9-BFP-KRAB (Addgene, #46911) is used ...
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bioRxiv - Cell Biology 2024Quote: ... OPTN1-209-GFP (Addgene, plasmid #68839 [41]), OPTN217-577-GFP (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... Three gRNAs were derived from the sequences to be deleted in the insertion were expressed in a modified pCFD3 vector (Addgene). The homology and gRNA vectors were co-injected into w- embryos ...
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bioRxiv - Synthetic Biology 2024Quote: ... with 10ug of Tol2 transposase plasmid (pCMV-Tol2 Addgene # #31823) and 10 ug of Path_Var library ...
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bioRxiv - Synthetic Biology 2024Quote: ... The base Cas9 CDS was amplified from ABE8e plasmid (Addgene #138489) using the oligonucleotides BE_Npu_F and BE_pJC175e_R ...