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Citations for Addgene :
4901 - 4950 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... were purchased from Addgene. The sources and sequences of all nucleic acids used in this study are summarized in Supplementary Data 2.
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-CD14 was a gift from Doug Golenbock (Addgene plasmid # 13645; http://n2t.net/addgene:13645; RRID:Addgene_13645). We designed primers to truncate CD14 at residue 367 (truncation at this residue to produce soluble CD14 has been previously reported (46) ...
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bioRxiv - Biophysics 2024Quote: ... Der (Addgene plasmid #53761 ...
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bioRxiv - Cancer Biology 2024Quote: ... OVCAR3 cells were transfected with pCAG-H2B-GFP plasmid (Addgene #184777) using InvitrogenTM LipofectamineTM 2000 Transfection Reagent following the manufacturer’s protocol and a stable cell line was established ...
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bioRxiv - Cancer Biology 2024Quote: ... cells carrying inducible Cas9 and the WNK1 sgRNAs were infected with either EGFP-expressing lentiviral particles (FUGW-EGFP, Addgene #14883) or FUGW-mRuby3-expressing lentiviral particles (34) ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiCRISPRv2 constructs were independently packaged into lentiviral particles in HEK293T cells with psPAX2 (Addgene cat. 12260) and pMD2.G (Addgene cat ...
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bioRxiv - Cancer Biology 2024Quote: ... into pLenti-CMV/TO-puroR-DEST (Addgene cat. 17293). To regulate expression of the CMV/TO-AXL transcript ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (Addgene cat. 12259), according to the Lipofectamine 2000 protocol (Invitrogen cat ...
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bioRxiv - Cancer Biology 2024Quote: ... we utilized the lentiCRISPRv2 vector (Addgene cat. 52961) with one of two AXL-targeted sgRNAs (Oligo 1 ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid psPAX2 (Addgene plasmid #12260), and envelope plasmid pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cancer Biology 2024Quote: ... the LentiCRISPRv2 (Addgene plasmid #52961) vector was digested with BsmBI and ligated with BsmBI-compatible pre-annealed oligonucleotides (Sanjana et al. ...
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bioRxiv - Cell Biology 2024Quote: cDNA for CENP-T full-length sequence was cloned into pAID1.2-CMV-NmScarlet-mAID (Addgene; #140618). To express the OsTIR1-T2A-BSR and mScarlet-mAID-CENP-T under control of the CMV promoter from the PGK locus ...
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bioRxiv - Cell Biology 2024Quote: ... Smo-mCherry (Addgene plasmid #55134), pGEM-Heh_bPAC-myc (Addgene plasmid #28134 gift from Peter Hegemann ...
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bioRxiv - Cancer Biology 2024Quote: The shRNA sequences were inserted into the pLKO.TRC.1 plasmid (Addgene, Cat#10878, RRID: Addgene_10878, Addgene).
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bioRxiv - Cell Biology 2024Quote: ... vector with the hPGK promoter amplified from pLenti.PGK.blast-Renilla_Luciferase (Addgene 74444) using AseI and NheI ...
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bioRxiv - Cell Biology 2024Quote: ... LBR pEGFP-N2 (646) (Addgene #61996); pFKBP-GFP-C1 (Clarke and Royle ...
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bioRxiv - Cell Biology 2024Quote: ... pMaCTag-P05 (Addgene #120016); pMito-mCherry-FRB (Addgene #59352) ...
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bioRxiv - Cell Biology 2024Quote: ... FKBP-GFP-Sec61β (Addgene #172442); FRB-mCherry-Giantin (Addgene #186575) ...
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bioRxiv - Cell Biology 2024Quote: ... pmScarlet-Giantin-C1 (Addgene #85048); SH4-FRB-mRFP (Addgene #100741) ...
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bioRxiv - Cell Biology 2024Quote: ... Emerin pEGFP-C1 (637) (Addgene #61993); FKBP-alpha(740-977)-GFP (Addgene #100731) ...
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bioRxiv - Cell Biology 2024Quote: ... LAP2 Full I pAcGFP-N1 monomeric GFP (1317) (Addgene #62044); LBR pEGFP-N2 (646 ...
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bioRxiv - Cell Biology 2024Quote: ... Stargazin-GFP-LOVpep (Addgene #80406); Stargazin-mCherry-FRB (Addgene #172443 ...
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bioRxiv - Cell Biology 2024Quote: ... SH4-FRB-mRFP (Addgene #100741); Stargazin-dCherry-FRB (Addgene #172444) ...
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bioRxiv - Cell Biology 2024Quote: ... Lamp1-mCherry-FRB (Addgene #186576); LAP2 Full I pAcGFP-N1 monomeric GFP (1317 ...
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bioRxiv - Cell Biology 2024Quote: ... FRB-mCherry-Giantin (Addgene #186575); GFP-EEA1 (Addgene #42307) ...
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bioRxiv - Cell Biology 2024Quote: ... pMito-dCherry-FRB (Addgene #186573); pmScarlet-Giantin-C1 (Addgene #85048) ...
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bioRxiv - Cell Biology 2024Quote: ... FKBP-alpha(740-977)-GFP (Addgene #100731); FKBP-GFP-Sec61β (Addgene #172442) ...
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bioRxiv - Cell Biology 2024Quote: The following plasmids were available from Addgene or previous work ...
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bioRxiv - Cell Biology 2024Quote: ... pMito-mCherry-FRB (Addgene #59352); pMito-dCherry-FRB (Addgene #186573) ...
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bioRxiv - Cancer Biology 2024Quote: ... gRNAs were cloned into pGL3-U6-PGK-Puro (Addgene 51133, a gift from Xingxu Huang) as previously described 36 ...
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bioRxiv - Cancer Biology 2024Quote: ... a gift from David Root and John Doench (Addgene 73178). The plasmid library was propagated ...
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bioRxiv - Cell Biology 2024Quote: ... and ITSN1-tgRFPt-SspB were PCR-amplified from pLL7.0:Venus-iLID-CAAX (Addgene #60411 ...
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bioRxiv - Cell Biology 2024Quote: ... we used a plasmid that was a gift from Sandra Schmid (Addgene plasmid # 34680; http://n2t.net/addgene:34680; RRID: Addgene_34680)27 ...
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bioRxiv - Cell Biology 2024Quote: The plasmids used for the luciferase assay were generated by cloning 1 kb of either the human HSF2 promoter or MMP9 promoter upstream of the respective TSS into a STARR-seq luciferase validation vector_ORI_empty vector (#99297, Addgene). The DNA from HDFs was isolated by first lysing cells in 3 ml lysis buffer (10 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... pMD2.G (Addgene, 12259) and psPAX2 (Addgene ...
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bioRxiv - Cell Biology 2024Quote: ... mScarlet-i-PLEKHA5WW was made by inserting the cDNA sequence encoding the tandem WW domain (residue 1-105) into the CMV-mScarlet-i-C1 vector (Addgene 85044) using BamHI and SalI ...
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bioRxiv - Cell Biology 2024Quote: ... a gift from the De Camilli lab at Yale University) into the miRFP670-N1 vector (Addgene 79987) using SalI and ApaI.
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bioRxiv - Cell Biology 2024Quote: ... Plasmids used were coding for HA-tagged KDM4A/JMJD2A (Addgene #24180) or for an irrelevant HA-tagged construct HA-Tsg5’ as negative control (24).
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bioRxiv - Cell Biology 2024Quote: ... 5HT6-G-GECO (Addgene plasmid #47499), Epac-SH187 and Epac-SH188 (gift from Kees Jalink ...
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bioRxiv - Cell Biology 2024Quote: ... followed by digestion of amplified PCR product with AgeI and NotI and ligation C-terminal to Smo in Smo-mCherry (Addgene plasmid 55134 ...
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bioRxiv - Cell Biology 2024Quote: H2B-tagBFP was cloned by inserting the H2B cDNA (amplified from pLenti6-H2B-mCherry, Addgene 89766) into the tagBFP-N1 vector (Evrogen ...
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bioRxiv - Cell Biology 2024Quote: ... Then the entire Lyn10-TurboID-V5 cassette was inserted into the pSBtet-Pur plasmid (Addgene 60507) linearized by NcoI and BspDI to replace the firefly luciferase downstream of the Tet-responsive promoter ...
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bioRxiv - Cell Biology 2024Quote: ... pCDNA3-Lyn10-TurboID-V5 was cloned by replacing the C1(1-29) sequence in C1(1-29)-TurboID-V5-pCDNA3 (Addgene, 107173) with a Lyn10 tag (amino acid sequence GCIKSKRKDG) ...
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bioRxiv - Cell Biology 2024Quote: Plasmids expressing GST-RILP (obtained from Christopher Burd, Yale University) in pGEX-KG vector (Addgene, 77103) or pGEX KG expressing GST alone was transformed into E.coli strain BL21 (DE3) ...
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bioRxiv - Cell Biology 2024Quote: ... AAV-PHP.S-tdTomato (Addgene, 59462- PHP.S, titer ≥ 1×10¹³ vg/mL) was utilized ...
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bioRxiv - Cancer Biology 2024Quote: ... then transfected with Lipofectamine 3000 according to the manufacturer’s protocol with 2.5 µg of a plasmid containing Sleeping Beauty transposase (SB100X, AddGene #34879) and 2.5 µg of a plasmid containing either GFP or mCherry on a nuclear localization signal (NLS ...
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bioRxiv - Cancer Biology 2024Quote: ... and transfected with 2.5 µg linearized RUNX1 HDR template and 4 µg each of pCW-Cas9 plasmid (Addgene, #50661) as well as two sgRNA plasmids using program CD-118 of the 4D Nucleofector system (Lonza) ...
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bioRxiv - Cell Biology 2024Quote: pGEX-KG-D4H*-mCherry plasmid (Addgene #134604) was used to purify D4H*-mCherry as previously described (32) ...
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bioRxiv - Cell Biology 2024Quote: ... psPax2 (Addgene #12260) and pMD2.G (Addgene #12259 ...
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bioRxiv - Cell Biology 2024Quote: ... and pMD2.G (Addgene #12259) using 1 mg/ml polyethylenimine ...