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Citations for Addgene :
4651 - 4700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... We replaced YFP with HaloTag and mScarletI (from Addgene #85065) and subcloned the full cORF in AAV backbone at SpeI and HindIII sites to generate pAAV hSyn INTRON HaloTag-STIM2 WPRE and pAAV hSyn INTRON mScarlet-STIM2 WPRE ...
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bioRxiv - Molecular Biology 2024Quote: ... a gift from Markus Landthaler (Addgene plasmid # 97060; http://n2t.net/addgene:97060; RRID: Addgene 97060) and ligation into pLVX-IRES-Hygro ...
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bioRxiv - Biochemistry 2024Quote: Synthetic complementary oligos targeting POLDIP2 (5’-CACCGCGCGTCGTCGTGGTCGACGC-3’ and 5’-AAACGCGTCGACCACGACGACGCGC -3’) were cloned into PX459-SpCas9 (Addgene, (35)) at the BbsI site ...
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bioRxiv - Biochemistry 2024Quote: ... and StcEE447D were expressed in-house as previously described.9,52 The plasmid for PNGaseF expression was purchased from Addgene (Addgene, 114274). PNGaseF was expressed in-house as described and was used for glycopeptide enrichments from complex samples.67
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bioRxiv - Biochemistry 2024Quote: ... and StcEE447D were expressed in-house as previously described.9,52 The plasmid for PNGaseF expression was purchased from Addgene (Addgene, 114274). PNGaseF was expressed in-house as described and was used for glycopeptide enrichments from complex samples.67
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-EGFP-RhoA-Q63L was a gift from Gary Bokoch (Addgene plasmid # 12968; http://n2t.net/addgene:12968; RRID:Addgene_12968). pcDNA3-EGFP-Rac1-Q61L was a gift from Gary Bokoch (Addgene plasmid # 12981 ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA3-EGFP-Rac1-Q61L was a gift from Gary Bokoch (Addgene plasmid # 12981 ...
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bioRxiv - Biochemistry 2024Quote: ... ASS1 and SLC7A5 cDNA in the vectors plX304 (Addgene #25890) and plX307 (#41392) ...
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bioRxiv - Biochemistry 2024Quote: ... bearing the gene for membrane scaffold protein expression was a gift from Stephen Sligar (Addgene plasmid # 20066) and was expressed as described before43 ...
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bioRxiv - Neuroscience 2024Quote: ... with the cap6 gene in pRepCap6 (gifted from David Russell [Addgene plasmid # 110770; http://n2t.net/addgene:110770; RRID:Addgene_110770]). These gene engineering experiments were approved by the Institutional Committee of Gunma University (approval No ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/5 (gifted from Melina Fan [Addgene plasmid # 104964 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV2/5 (gifted from Melina Fan [Addgene plasmid # 104964; http://n2t.net/addgene:104964; RRID:Addgene_104964]), pAAV2/7 (gifted from James M ...
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bioRxiv - Neuroscience 2024Quote: ... and pAAV2/AAVrh10 (gifted from James M. Wilson [Addgene plasmid # 112866; http://n2t.net/addgene:112866; RRID:Addgene_112866]) were obtained from Addgene ...
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bioRxiv - Biochemistry 2024Quote: ... FLAG-tagged TDP43 WT or mutant coding DNA sequence (CDS) was PCR amplified and cloned into a doxycycline-inducible pCW vector (Addgene #50661) at NheI (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 1ug of DNA was cotransfected with 1ug BxB1 recombinase (pCAG-NLS-BxB1, Addgene #51271) using 3.75uL lipofectamine 3000 and 5uL P3000 reagent in 6 wells of a 6 well plate ...
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bioRxiv - Microbiology 2024Quote: ... mCherry-2xFYVE plasmid was obtained from Addgene (#140050). SARS-CoV-2 subgenomic ΔS-E-M replicon was provided by Dr ...
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bioRxiv - Genetics 2024Quote: ... subtilis single gene deletion library--kanamycin was acquired from Addgene (Watertown, MA, 1000000115). The parent strain was acquired from the Bacillus Genetic Stock Center (Columbus ...
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bioRxiv - Neuroscience 2024Quote: ... 0.6-0.8 uL of a 1:1 mixture of GAD1-cre and mCherry virus (AAV8-hSyn-DIO-mCherry, ≥ 1×101 3 vg/mL; Addgene, Watertown, MA, USA) was injected bilaterally into VP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... mouse RIPK1 (Addgene #115341), and mouse RIPK3 (Addgene #78805 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The protocol for the molecular cloning of this plasmid was adapted from Feng Zhang (Sanjana et al., 2014) using the transfer plasmid pLB-Cas9 (gift from Feng Zhang, Addgene plasmid # 52962). We designed the gRNA target sequence using the web tool CHOPCHOP (Labun et al. ...
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bioRxiv - Neuroscience 2024Quote: ... Gq DREADD virus (n=23, 12 males, 11 females: AAV8-hSyn-DIO-hM3Dq-mCherry, ≥ 4×101 2 vg/mL, Addgene; Watertown, MA, USA) was mixed with GAD1-cre to express excitatory designer receptors in VP GABA neurons ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or the human IFN-beta promoter (IFN-Beta-pGL3, Addgene #102597), renilla luciferase fused to herpes simplex virus thymidine kinase promoter (pTK-Renilla ...
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bioRxiv - Evolutionary Biology 2024Quote: ... human RIPK3 (Addgene #78804), mouse RIPK1 (Addgene #115341) ...
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bioRxiv - Neuroscience 2024Quote: ... and the duplexes were cloned into PX330 (Addgene, #158973) by BbsI site ...
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bioRxiv - Neuroscience 2024Quote: ... after which they were injected with adeno-associated virus (AAV-PHP.eB-syn-jGCaMP7s, Addgene) using a 30-gauge needle ...
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bioRxiv - Neuroscience 2024Quote: ... and GRABACh3.0 (AAV9-hSyn-DIO-GRABACh3.0 titer: > 1x 1012, plasmid: Addgene #121923, virus packaged by Vigene). Viral aliquots were injected (0.1-0.5 μL ...
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bioRxiv - Neuroscience 2024Quote: ... The generated myo-3p::circ-crh-1 construct along with the unc-122p::RFP co-injection marker (AddGene) were injected into VDL1300 crh-1(syb385) ...
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bioRxiv - Neuroscience 2024Quote: ... and were a gift from Cheryl Arrowsmith (Addgene plasmid #111741 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV9.syn.flex.GcaMP6s (Addgene, Catalog# pNM V3872TI-R(7.5)) ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV5-hsyn-DIO-hM3D(Gq)-mCherry (Addgene, Catalog# v141469), pAAV5-hsyn-DIO-hM4D(GI)-mCherry (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... or mCherry alone as control (AAV5-hSyn1-DIO- mCherry , Addgene). Coordinates were relative to Bregma according to the Franklin and Paxinos atlas third edition ...
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bioRxiv - Neuroscience 2024Quote: ... EGFP-ESyt2 (#66831) and GFP-C1-PLCdelta-PH (#21179) were obtained from Addgene. The following constructs were kindly provided ...
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bioRxiv - Neuroscience 2024Quote: ... Plcxd2fl/fl:Rorb-Cre and Plcxd2+/+:Rorb- Cre pups were injected with 50nL of a mixture containing AAV-TRE-DIO-FLPo (Addgene #118027) and AAV-TRE-fDIO-GFP-IRES-tTA (Addgene #118026 ...
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bioRxiv - Neuroscience 2024Quote: ... and AAV9 ef1a-flex-Tdtomato viral vectors were purchased from the Canadian Neurophotonics Platform Viral Vector Core Facility (RRID:https://identifiers.org/RRID/RRID:SCR_016477) and AAVrg-flex- Tdtomato was purchased from Addgene (viral prep: 28306-AAVrg). Retrogradely infecting AAVs were used for brain injections while AAV9 was used for intravitreal injections.
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bioRxiv - Neuroscience 2024Quote: ... and AAV-SYN-DIO-GFP-IRES-tTA (Addgene #85006) in a 1:50 ratio in the barrel cortex ...
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bioRxiv - Neuroscience 2024Quote: ... Gpr158fl/fl:Plcxd2+/+ and Gpr158fl/fl:Plcxd2fl/fl pups were injected with 50nL of a mixture containing AAV-TRE- Cre (Addgene #69136) and AAV-SYN-DIO-GFP-IRES-tTA (Addgene #85006 ...
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bioRxiv - Evolutionary Biology 2024Quote: Coding sequences for human RIPK1 (Addgene #78834), human RIPK2 (ORFeome ID #4886) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were assembled using gene sequences ordered from IDT (gBlocks) and plasmid backbones (ordered from Addgene or derived from an existing Addgene plasmid) using the NEB Gibson Assembly Master Mix (#E2611) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All plasmids generated in this study (with the exception of pWM17) were assembled using gene sequences ordered from IDT (gBlocks) and plasmid backbones (ordered from Addgene or derived from an existing Addgene plasmid ...
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bioRxiv - Immunology 2024Quote: ... Two BA.5 NTD DMS libraries were further assembled into pETcon 2649 vector (Addgene) and electroporated into electrocompetent DH10B cells for plasmid amplification ...
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bioRxiv - Neuroscience 2024Quote: Vector used and sources: pAAV5-hsyn-DIO-EGFP (Addgene, Catalog# 50457-AAV 1), pAAV5-FLEX-tdTomato (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV5-hsyn-DIO-hM4D(GI)-mCherry (Addgene, Catalog# 44362-AAV5), AAV9.syn.flex.GcaMP6s (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... HyPer biosensor 3.0 under CMV promoter (Addgene, #42131). We transfected 10.4 μg of pFΔ6 ...
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bioRxiv - Neuroscience 2024Quote: ... a plasmid with the synapsin-1 promoter that controls the expression of the biosensor GCaMP6 and fused to the red fluorochrome mRuby2 (Addgene, # 50942); and hSyn-HyPer ...
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bioRxiv - Neuroscience 2024Quote: ... and were a gift from Cheryl Arrowsmith (Addgene plasmid #111741; http://n2t.net/addgene:111741; RRID:Addgene_111741 and Addgene plasmid #111730 ...
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bioRxiv - Neuroscience 2024Quote: ... and were a gift from Cheryl Arrowsmith (Addgene plasmid #111741; http://n2t.net/addgene:111741; RRID:Addgene_111741 and Addgene plasmid #111730 ...
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bioRxiv - Neuroscience 2024Quote: All viral vectors were either purchased from Addgene.org ...
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bioRxiv - Neuroscience 2024Quote: AAV9-hSyn-HI.eGFP-Cre (Addgene 105540-AAV9; titer: 5.44e11 vg/mL)
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bioRxiv - Neuroscience 2024Quote: AAV9-hSyn-GFP (Addgene 50465-AAV9; titer: 1.9e11 vg/mL)
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bioRxiv - Neuroscience 2024Quote: ... Rats were then micro-injected bilaterally with 0.5 μl of either the AAV8-CAMKII-hM4Di-mCherry or AAV8-CAMKII-EGFP control viruses (Addgene, MA) into the vCA1 (AP ...