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Citations for Addgene :
4851 - 4900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... the GFP nanobody sequence was amplified from pUAST-attB-NSlmb-vhhGFP4 (Caussinus et al., 2012, Addgene 35577) and fused to the Ed ECD as just described ...
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bioRxiv - Developmental Biology 2024Quote: ... and restriction cloned into the lentiGuide-puro105 (Addgene: 52963; RRID: Addgene_52963) backbone by the MSKCC Gene Editing & Screening Core Facility.
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bioRxiv - Genetics 2024Quote: ... a modified version of PLGR002 which contains a second guide cassette (Addgene, 188320). Both guides within a vector were designed to target either ...
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bioRxiv - Genetics 2024Quote: ... Lentivirus was produced from sgRNA plasmids by co-transfecting Lenti-X 293T (Takarabio, 632180) cells with the sgRNA plasmid library and packaging plasmids-psPAX2 and pMD2.G (Addgene, 12260 and 12259) using Trans-IT-293 transfection reagent (Mirus ...
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bioRxiv - Genetics 2024Quote: ... The sequence-verified expression constructs for wild-type and mutated AARS1 were then cloned into either the pAG425 expression vector (pAG425GAL-ccdB; Addgene #14153 ...
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bioRxiv - Developmental Biology 2024Quote: ... David Sabatini and Kris Wood (Addgene plasmid #64613 ...
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bioRxiv - Developmental Biology 2024Quote: ... The Cas12a fragment was cloned from the pY016 plasmid (Addgene: #69988) and the Cas9-ERT2 part was cloned from the HIT-Cas9 plasmid (a gift from Dr ...
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bioRxiv - Developmental Biology 2024Quote: ... we cloned canine Podxl1 into the pQCXIZ retroviral plasmid with a GFP or RFP fused to the N-terminus (a gift from Eric Campeau, Addgene plasmid #22801). We packaged retroviruses containing each vector in HEK293GPG using Mirus Trans-ItLT1 transfection reagent ...
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bioRxiv - Developmental Biology 2024Quote: ... tdTomato was amplified from pCAG-tdTomato (Addgene #83029, gift from Angelique Bordey), Cry2 was amplified from pBI-UASp-mCherry-Cry2phr-RhoGAP71E (Herrera-Perez et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... SspB and tgRFPt were amplified from pLL7.0: tgRFPt-SSPB WT (Addgene #60415, gift from Brian Kuhlman), tdTomato was amplified from pCAG-tdTomato (Addgene #83029 ...
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bioRxiv - Developmental Biology 2024Quote: ... DsRed was amplified from pScarlessHD-sfGFP-DsRed (Addgene #80811, gift from Kate O’Connor-Giles), and homology arms were amplified from genomic DNA of vas-Cas9 III (BDSC #51324) ...
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bioRxiv - Developmental Biology 2024Quote: ... The plasmid pTol2-TP1bglob-eGFP (TP1:GFP in this study) was obtained from Addgene (plasmid 73586). The plasmid pTol2-TP1-SpCas9-t2A-GFP ...
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bioRxiv - Developmental Biology 2024Quote: ... and restriction cloned into the lentiGuide-puro105 (Addgene: 52963; RRID: Addgene_52963) backbone by the MSKCC Gene Editing & Screening Core Facility.
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bioRxiv - Developmental Biology 2024Quote: ... The synthesized gRNA sequences were annealed and cloned into pU6-BbsI-chiRNA vector (Addgene #45946). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... The final constructs were generated via multisite Gateway with p5E-TP1 (Addgene plasmid 73585) or p5E her4.3-loxP-dsRed-SV40pA-loxP together with pME-Cas9-t2A-GFP (Addgene plasmid 63155) ...
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bioRxiv - Developmental Biology 2024Quote: ... Fragments were cloned into a pScarlessHD-sfGFP-DsRed donor vector (Addgene #80811, gift from Kate O’Connor-Giles) using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs ...
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bioRxiv - Developmental Biology 2024Quote: ... mEos3.2 and green-to-red photoconvertible protein (a gift from Michael Davidson & Tao Xu (Addgene plasmid 54525) was coupled to Histone 2B (H2B ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid mEmerald-N1 was a gift from Michael Davidson (Addgene plasmid #53976). Plasmids pDsChLexOG (Emelyanov and Parinov ...
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bioRxiv - Genetics 2024Quote: ... and NGN2 (Addgene 99378) lentiviruses in StemFlex media containing 10 μM Thiazovivin (Millipore ...
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bioRxiv - Genetics 2024Quote: ... Barcoded fragments were inserted in the SbfI/AgeI site of the pLS-SceI vector (AddGene #13772) and then transformed into 10-beta competent cells (NEB ...
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bioRxiv - Genetics 2024Quote: ... To determine MOI and approximation of appropriate viral volume we infected day 14 iGLUTs (0, 1, 2, 4, 8, 10, 16, 32, 64 µL) with control lentivirus (pLS-SV40-mP-EGFP; AddGene #137724) and harvested for 48hrs later ...
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bioRxiv - Genetics 2024Quote: Clonal hiPSCs from two neurotypical donors of European ancestry with average schizophrenia PRS and no history of psychiatric diagnoses (#3182 (XX) and #2607 (XY)) were generated by lentiviral transduction with pLV-TetO-hNGN2-eGFP-Neo and lentiviral FUW-M2rtTA (Addgene #20342), followed by antibiotic selection and clonal expansion ...
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bioRxiv - Genetics 2024Quote: ... acquired from Addgene (plasmid # ...
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bioRxiv - Genetics 2024Quote: We used p-dCas9-SSAP-MS2-BB (Addgene Cat#183826) [62] as a convenient source of bacterial backbone and dCas9 for Gibson assembly ...
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bioRxiv - Genetics 2024Quote: ... You can use our plasmid (deposited at Addgene as dCas9-ABE8e-Blasticidin-MC-Plasmid #XXXXXXX ...
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bioRxiv - Developmental Biology 2024Quote: ... we first made a targeting construct using CRE-treated and BstBI/MluI digested LSL-Cas9-ROSA26TV plasmid donor (gift from Feng Zhang, #61408, Addgene) to subclone the DHB-mClover3_2A_H2B-mRuby3 fragment ...
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bioRxiv - Developmental Biology 2024Quote: ... were amplified by PCR and ligated them by Gibson cloning using the plasmid backbone from CTCF-mAC donor (gift from Masato Kanemaki, #140646, Addgene). ESC were co-transfected using jetPRIME (PolyPlus transfection ...
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bioRxiv - Developmental Biology 2024Quote: ... ESC were co-transfected using jetPRIME (PolyPlus transfection) with the targeting vector as well as with LentiCRISPRv2 (gift from Feng Zhang, #52961, Addgene) encoding a sgRNA designed to target the ROSA26 or CDK1 ...
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bioRxiv - Genetics 2024Quote: ... The plasmids pDB5318- pDB5322 have been deposited at Addgene (Addgene plasmids 204828-204832) and the Yeast Genetic Resource Center of Japan (YGRC/NBRP ...
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bioRxiv - Developmental Biology 2024Quote: ... we utilized the pCAG-GFP vector59 (pCAG-GFP was a gift from Connie Cepko; Addgene #11150). GFP was excised with EcoRI and NotI digestion and a synthesized Mest211 cDNA gBlock (Genbank ...
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bioRxiv - Developmental Biology 2024Quote: ... we first generated a lentiviral vector to constitutively express the transgene DHB-mClover3_2A_H2B-mRuby3 by cloning this fragment into BamHI/EcoRI digested FUGW (Gift from David Baltimore, #14883, Addgene). TSC were trypsinized and infected in suspension with infective lentiviral particles ...
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bioRxiv - Cell Biology 2024Quote: ... NHEKs were transduced with ERK-KTR-mClover (Addgene Cat. #59150). HEK293FT cells were grown in complete DMEM ...
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bioRxiv - Cell Biology 2024Quote: ... RFP-EEA1 (TagRFP-T-EEA1 cloned into pEGFP-C1 vector) and HA-Dyn-K44A (cloned into pcDNA3.1) constructs were gifts from Silvia Corvera and Sandra Schmid (respectively Addgene plasmids #42635 and #34683). RFP-Lck (C-tRFP-Lck cloned into PCMV6-AC-RFP expression vector ...
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bioRxiv - Cell Biology 2024Quote: ... Hoechst 33342 came from Thermo-Fisher (Cat. #H1399). The ERK fluorescent biosensor (pLenti-CMV-Puro-DEST-ERK-KTR-mClover; Cat. #59150) was from Addgene.
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bioRxiv - Genetics 2024Quote: ... which was modified from a pCAS plasmid (Addgene # 60847) by replacing the gRNA cloning sequence with an XbaI and ZraI fragment by Robert Gnügge of this laboratory ...
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bioRxiv - Genetics 2024Quote: ... This amplified promoter was then cloned between the SwaI and XhoI sites in the stable integration vector (SIV) pAV0714 (Addgene plasmid 133479), replacing the act1 promoter (Vještica et al. ...
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bioRxiv - Genetics 2024Quote: ... pDB4915 was derived from the SIV plasmid pAV0585 (pAde6PmeI-hphMX, Addgene plasmid 133472) (Vještica et al. ...
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bioRxiv - Genetics 2024Quote: ... and pDB5538 were derived from the SIV plasmid pAV0586 (pLys3BstZ17I-bsdMX, Addgene plasmid 133473), by altering the linearization site from BstZ17I to NotI and introducing the TetR expression cassette ...
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bioRxiv - Genetics 2024Quote: ... and pDB4879 were derived from the SIV vector pAV0585 (pAde6PmeI-hphMX, Addgene plasmid 133472) (Vještica et al. ...
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bioRxiv - Genetics 2024Quote: ... we amplified a TetR expression cassette containing the cytomegalovirus promoter (P.CMV) and the coding sequence of TetR from pDM291-tetR-tup11Δ70 (Addgene plasmid 41027) (Zilio et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... a gift from Dorus Gadella (Addgene plasmid # 112962). pT7T-C-cadherin-mYFP was modified from pT7T-CcadTSMod ...
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bioRxiv - Developmental Biology 2024Quote: ... 293T cells were transfected with 2 μg of pMD2.G (Addgene, 12259), 4 μg of psPAX2 (Addgene ...
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bioRxiv - Developmental Biology 2024Quote: ... 4 μg of psPAX2 (Addgene, 12260), and 6 μg of GIPZ Non-silencing Lentiviral shRNA (control ...
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bioRxiv - Developmental Biology 2024Quote: ... a gift from Marc Tramier (Addgene plasmid # 99874)(Herbomel et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882) through site-directed mutagenesis using Neb Q5 high-fidelity polymerase (New England Biolab #M0491) ...
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bioRxiv - Developmental Biology 2024Quote: The plasmid encoding wild-type Yap was obtained from Addgene (pcDNA Flag Yap1, Addgene #18881). Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882 ...
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bioRxiv - Developmental Biology 2024Quote: The plasmid encoding wild-type Yap was obtained from Addgene (pcDNA Flag Yap1, Addgene #18881). Plasmids encoding Yap2YF and Yap3YF were constructed from pcDNA Flag-Yap1 Y357F (Addgene #18882 ...
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bioRxiv - Developmental Biology 2024Quote: ... The gene sequences encoding N-terminally mCherry tagged Drp1 (Addgene, 49152) were inserted between the EcoRI and PstI sites of the pCDNA vector (mCherry-Drp1) ...
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bioRxiv - Developmental Biology 2024Quote: ... and peroximal targeting signal 1 (Addgene, 54520) were cloned into mCherry-bearing pcDNA6 (Golgi-mCherry and PEX-mCherry) ...
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Leveraging single cell multiomic analyses to identify factors that drive human chondrocyte cell fatebioRxiv - Developmental Biology 2024Quote: ... NFATC2 CDS was transferred into the pInducer20 lentivirus destination vector (Addgene Plasmid #4401252) using Gateway LR Clonase to generate pInducer20-NFATC2.