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Citations for Addgene :
1651 - 1700 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... 0.9 μg of psPax2 (Addgene 12260), 0.2 μg of pMD2.G (Addgene 12259 ...
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bioRxiv - Genomics 2024Quote: ... a gift from David Root and John Doench (Addgene #73178) 33 ...
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bioRxiv - Genomics 2024Quote: ... which was a gift from Christoph Bock (Addgene #86708), was PCR-amplified with primers CROPseq_iT7_fwd/rev using Q5 polymerase ...
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bioRxiv - Immunology 2024Quote: ... and pMD2.G (from Didier Trono, Addgene plasmid #12259) and were used for production of lentivirus [19] ...
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bioRxiv - Immunology 2024Quote: ... targeting CYLD was cloned by annealing two DNA oligos (forward, 5’-GTGGTCAAGGTTTCACTGAC-3’; reverse, 5’-GTCAGTGAAACCTTGACCAC-3’) and ligating into lentiCRISPER v2 (52961, Addgene) plasmids ...
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bioRxiv - Immunology 2024Quote: Lentiviral transfer plasmids encoding different transgenes were constructed into the pHR vector (modified from Addgene #79125) by NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Immunology 2024Quote: ... Human Kinome CRISPR Knockout Library was from Addgene (catalog #1000000082) and prepared as described (Doench et al. ...
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bioRxiv - Immunology 2024Quote: ... 500 ng of pCMV-VSV-G (Addgene plasmid #8454) and 1000 ng of cloned LentiCRISPRv2-Puro plasmid using Lipofectamine (5µl P3000 ...
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bioRxiv - Immunology 2024Quote: ... One microliter of 1:100 diluted product was used for golden gate cloning into lentiCRISPR v2-Puro (Addgene plasmid #52961) using 11 cycles of 5 minutes T4 ligase ligation at 16 °C and 5 minutes of BsmbI digestion at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... Lentiviral packaging vectors pMD2.G (Addgene 12259) and psPAX2 (Addgene 12260 ...
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bioRxiv - Genomics 2024Quote: ... were combined with CROPseq-Puro-F+E plasmid (containing the sgRNAs) or dCas9-mCherry-ZIM3-KRAB plasmid (Addgene 154473) and transfected using Lipofectamine™ 3000 (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... and psPAX2 (Addgene 12260) were combined with CROPseq-Puro-F+E plasmid (containing the sgRNAs ...
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bioRxiv - Immunology 2024Quote: ... Packaging plasmids psPAX2 (from Didier Trono, Addgene plasmid #12260) and pMD2.G (from Didier Trono ...
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bioRxiv - Immunology 2024Quote: ... MEFs were transfected with SV40 small+large T antigen-expressing plasmid (22298, Addgene) using Lipofectamine 2000 (11668019 ...
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bioRxiv - Immunology 2024Quote: ... 200 ng of envelope plasmid (Addgene #8454; courtesy of Bob Weinberg), and 6 of μL polyethyleneimine (PEI ...
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bioRxiv - Immunology 2024Quote: ... 800 ng of packaging plasmid (Addgene #8455; courtesy of Bob Weinberg), 200 ng of envelope plasmid (Addgene #8454 ...
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bioRxiv - Immunology 2024Quote: ... OVA sequence from Addgene (#22533) was used.
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bioRxiv - Immunology 2024Quote: Stably expressing H2B-GFP RPE1 WT and SP110-/- cells were generated using lentivirus generated from Addgene plasmid #91788 (Courtesy of Beverly Torok-Storb) ...
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bioRxiv - Microbiology 2024Quote: ... The viral gene constructs used in this study were sourced as follows: pGwf and phGf were from Addgene; pALTOw ...
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bioRxiv - Immunology 2024Quote: ... lentiCRISPRv2 was a gift from Feng Zhang (Addgene plasmid# 52691 ...
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bioRxiv - Immunology 2024Quote: ... psPAX2 was a gift from Didier Trono (Addgene plasmid# 12260 ...
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bioRxiv - Immunology 2024Quote: ... psPAX2 was a gift from Didier Trono (Addgene plasmid# 12260 ; http://n2t.net/addgene:12260; RRID:Addgene_12260). Oligomycin A (cat.# ...
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bioRxiv - Immunology 2024Quote: ... pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid# 131626 ...
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bioRxiv - Immunology 2024Quote: ... pEBB-HA cIAP1 was a gift from Colin Duckett (Addgene plasmid# 38232; http://n2t.net/addgene:38232; RRID:Addgene_38232). lentiCRISPRv2 was a gift from Feng Zhang (Addgene plasmid# 52691 ...
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bioRxiv - Immunology 2024Quote: ... pUltra-Hot was a gift from Malcolm Moore (Addgene plasmid# 24130 ...
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bioRxiv - Immunology 2024Quote: ... pHAGE-mt-mKeima was a gift from Richard Youle (Addgene plasmid# 131626; http://n2t.net/addgene:131626 ; RRID:Addgene_131626). ZFAND6-Flag-Myc was purchased from Origene ...
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bioRxiv - Immunology 2024Quote: ... lentiCRISPRv2 was a gift from Feng Zhang (Addgene plasmid# 52691 ; http://n2t.net/addgene:52691; RRID:Addgene_52691). pUltra-Hot was a gift from Malcolm Moore (Addgene plasmid# 24130 ...
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bioRxiv - Immunology 2024Quote: ... pEBB-HA cIAP1 was a gift from Colin Duckett (Addgene plasmid# 38232 ...
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bioRxiv - Immunology 2024Quote: ... pUltra-Hot was a gift from Malcolm Moore (Addgene plasmid# 24130 ; http://n2t.net/addgene:24130 ; RRID:Addgene_24130). pUltra-Hot-ZFAND6 was generated by subcloning a ZFAND6 PCR product digested with Xba1 (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: ... that itself was a polycistronic derivative of Feng Zhang’s dual vector system (Addgene 61422, Addgene, 73797). Successive daughter plasmids were generated via simple ligation or Gibson assembly using annealed complementary oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Immunology 2024Quote: ... The entire coding sequence of the CAR expression molecule was cloned and inserted into the lentiviral vector pTomo (#26291, Addgene, USA) and expressed under the control of the CMV promoter ...
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bioRxiv - Immunology 2024Quote: Our SAM.1 (PB-UniSAM) parent vector was a gift from Lesley Forrester (Addgene 99866), that itself was a polycistronic derivative of Feng Zhang’s dual vector system (Addgene 61422 ...
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bioRxiv - Immunology 2024Quote: ... norvegicus CD200 (Addgene #36152) as described for CIDRα1.
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bioRxiv - Microbiology 2024Quote: ... using an engineered plasmid that encodes a deGFP protein and a malachite green RNA aptamer (PT7-deGFP-MGapt was a gift from Richard Murray, via Addgene plasmid # 67741 ...
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bioRxiv - Microbiology 2024Quote: ... coli MG-1655 cells harboring the pEB1-mGFPmut2 plasmid where used throughout the study (pEB1-mGFPmut2 was a gift from Philippe Cluzel, Addgene plasmid #103980 ...
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bioRxiv - Microbiology 2024Quote: ... pRL0081 was generated from pRL0027 by cloning the Tet-On promoter from pdCas9-bacteria (Addgene catalog number 44249) along with rparr-2 and gfpuv under the control of Tet-ON with a single ompA terminator sequence ...
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bioRxiv - Microbiology 2024Quote: ... BQ.1.1(Addgene #170442 ...
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bioRxiv - Microbiology 2024Quote: ... firefly luciferase reporter (Addgene #170674) and S protein expressing plasmids of different SARS-CoV-2 variants - Wuhan ...
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bioRxiv - Microbiology 2024Quote: ... pET-6xHis-SUMO-MarathonRT encoding MarathonRT was a gift from Anna Pyle (Addgene plasmid # 109029 ...
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bioRxiv - Microbiology 2024Quote: ... pET-6xHis-SUMO-MarathonRT encoding MarathonRT was a gift from Anna Pyle (Addgene plasmid # 109029; http://n2t.net/addgene:109029; RRID: Addgene_109029) and purified according to (PMID 29109157) ...
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bioRxiv - Microbiology 2024Quote: Plasmids for sulfatases were from Addgene (#13003 and #13004). Heparan sulfate sulfotransferase genes (in pDONR223 from the ORFeome v8.1 collection (Dharmacon) ...
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bioRxiv - Immunology 2024Quote: ... were transfected with 100ng MSCV plasmid (MSCV multiple cloning site IRES puroR 2A mCherry or GFP) and 10ng pVSVg plasmid (Addgene 8454) with Lipofectamine 2000 (Thermo) ...
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bioRxiv - Immunology 2024Quote: ... The pK18-hACE2 plasmid was obtained from Addgene (Cat No:149449) (31) ...
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FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteriabioRxiv - Microbiology 2024Quote: Plasmid pXY027-ftsZ-eGFP used for visualisation of the Z-ring was a gift from Jie Xiao (Addgene plasmid # 98915)33 ...
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bioRxiv - Immunology 2024Quote: ... 0.35 µg pMD2.G (Addgene, 12259), and 0.8 µg of transfer plasmid using 5.8 X-tremeGENE HP (Roche) ...
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bioRxiv - Immunology 2024Quote: ... mCherry-Rab11a (Addgene #55124, mCherry-Rab4a (Addgene #55125), pSpCas9(BB)–2A-GFP (pX458 ...
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bioRxiv - Immunology 2024Quote: Arf1-mCherry was cloned by replacing EGFP in the pEGFP-N1-Arf1 (Addgene #39554) plasmid with mCherry ...
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bioRxiv - Immunology 2024Quote: ... mCherry-Rab11a (Addgene #55124, mCherry-Rab4a (Addgene #55125), pSpCas9(BB)–2A-GFP (pX458 ...
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bioRxiv - Immunology 2024Quote: ... LAMP1-GFP (Addgene #34831), mCherry-Rab11a (Addgene #55124 ...
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bioRxiv - Immunology 2024Quote: ... pSpCas9(BB)–2A-puro (pX459) (Addgene, #48139).