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Citations for Addgene :
1751 - 1800 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... were cloned into CMV Blast DEST (706–1) (a gift from Eric Campeau & Paul Kaufman, Addgene plasmid #17451) expression vector resulting in plasmid AX581 ...
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bioRxiv - Microbiology 2024Quote: The human CRISPR (clustered regularly interspaced short palindromic repeats) “Brunello” lentiviral pooled library was purchased from Addgene. The library version in the lentiCRISPRv2 backbone was chosen ...
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bioRxiv - Microbiology 2024Quote: ... 3.6μg psPAX2 (Gag-Pol expression construct, a gift from Didier Trono (Addgene plasmid #12260), and 5μg of lentiviral expression constructs (pLenti CMV Blast DEST (706–1) ...
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bioRxiv - Microbiology 2024Quote: ... 10cm cell culture grade petri dishes of approximately 80% confluent 293T cells were transfected with 1.4μg pMD2.G (VSV-G envelope expressing plasmid, a gift from Didier Trono (Addgene plasmid #12259), 3.6μg psPAX2 (Gag-Pol expression construct ...
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bioRxiv - Microbiology 2024Quote: ... pAR18 was constructed in three steps: 1) pTargetF (obtained from Addgene) was linearized with the primer pair AR430/431 while the cas9 sgRNA sequence was replaced by the sacB gene including its native promoter (amplified with AR432/433 from pEP17-KM [44]) ...
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bioRxiv - Microbiology 2024Quote: ... AR426/427 was used for backbone amplification from p46Cpf1-OP2 (obtained from Addgene) and AR428/429 was used to amplify the p15A ori from a pACYC-derived vector ...
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bioRxiv - Microbiology 2024Quote: ... 2.4 µg and 9,6µg of the packaging plasmids pCMV-VSV-G (Addgene #8454) and psPAX2 (Addgene #12260 ...
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bioRxiv - Microbiology 2024Quote: ... and psPAX2 (Addgene #12260) respectively ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected with mCherry-CD36-C-10 (Addgene: Plasmid #55011), 5µg per 10cm dish or 0.5µg per well of a 6-well dish ...
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bioRxiv - Microbiology 2024Quote: ... The TFEB wild-type (WT-TFEB [plasmid #38119]) and mutant (Δ30-TFEB [plasmid #44445]) constructs were obtained from Addgene (deposited by Shawn Ferguson) and transfected into H1HeLa using the Lipofectamine 2000 transfection reagent ...
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bioRxiv - Microbiology 2024Quote: ... and 5.5 μg of psPAX2 (Addgene, Cat# 12260), into HEK293T cells (6×106 cells ...
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bioRxiv - Microbiology 2024Quote: ... The PCR fragment was subcloned in the BamHI and NotI site of pET-28a+GFP-CAMSAP1 CKK (Addgene #59033), which contains 6 histidine (His ...
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bioRxiv - Immunology 2024Quote: ... and the packaging vector psPAX2 was a kind gift from Didier Trono (Addgene plasmid # 12260).
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bioRxiv - Immunology 2024Quote: ... The plasmid expresses EGFP under the constitutive EF1α promoter and was a kind gift from Didier Trono (Addgene plasmid # 12254). pWPI-mBatf-GFP was generated by ligating a murine BATF encoding sequence using SwaI and PacI restriction sites upstream of an IRES sequence into the pWPI expression plasmid ...
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bioRxiv - Microbiology 2024Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988; [49] into pSBtet-pur (Addgene #60507; [50]). Cas9 was first cloned into pSBtet-pur using the following primers ...
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bioRxiv - Microbiology 2024Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988 ...
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bioRxiv - Immunology 2024Quote: ... MSCV-Cxcr6-IRES-GFP or pMSCV-IRES-mCherry (Addgene # 52114) and pCL-Eco (Addgene #12371 ...
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bioRxiv - Immunology 2024Quote: ... electroporated with 10 ng pON-mCherry plasmid (Addgene, US) in 200 µl ddH2O using 2-mm cuvettes at 2.5 kV and 200 ν for 4 ms ...
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bioRxiv - Immunology 2024Quote: ... The plasmid pcDNA3.1 SARS-CoV-2 S D614G was a gift from Jeremy Luban (Addgene plasmid # 158075; http://n2t.net/addgene:158075; RRID:Addgene_158075). The RBD amino acid sequence 328-537 encoded in this plasmid is identical to the sequence of RBD that we studied here ...
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bioRxiv - Immunology 2024Quote: ... pSpCas9(BB)–2A-GFP (pX458) (Addgene #48138), pSpCas9(BB)–2A-puro (pX459 ...
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bioRxiv - Immunology 2024Quote: ... mApple-Rab7a (Addgene #54945), LAMP1-GFP (Addgene #34831) ...
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bioRxiv - Immunology 2024Quote: ... sgRNA targeting FADD exon1 was cloned into the lentiCRISPR v2-Blast (Addgene #83480). This vector was packaged into lentivirus as described above ...
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bioRxiv - Neuroscience 2024Quote: iPS cells were transduced with a Lentivirus expressing GFP under activation of WNT signaling (Lentiviral-Top-dGFP reporter, Addgene plasmid #14715). Puromycin (1μg/ml ...
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bioRxiv - Microbiology 2024Quote: The p25.A12△TFA mutant strain was cloned using the Gateway Cloning system with the donor vector pDONR1K18ms (Addgene plasmid #72644) and the destination vector pDEST2T18ms (Addgene plasmid #72647).
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bioRxiv - Microbiology 2024Quote: ... and the destination vector pDEST2T18ms (Addgene plasmid #72647).
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bioRxiv - Cancer Biology 2024Quote: ... Prokaryotic plasmids encoding GST-fusion proteins were constructed using pGEX-4T-1 bacterial expression vector (27-4580-01, Addgene). Mutants of His- ...
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bioRxiv - Bioengineering 2024Quote: pET28a-SpyTag003-MBP (GenBank Accession no. MN433888, Addgene plasmid ID 133450), pET28-SpyTag003-mClover3 (Addgene plasmid ID 133453 ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-MBPX-MBPX-SpyTag003 (His6-MBPX-SSSGGSGGGSG linker-MBPX-SpyTag003; GenBank and Addgene deposition in progress), where MBPX is a variant of MBP with stronger maltose binding (Veggiani et al. ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-SnoopTag-AffiHER2-SpyTag (N-terminal His6–SnoopTag–anti-HER2 Affibody–SpyTag) (GenBank accession no. KU296975) (Addgene deposition in progress) (Brune et al. ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-nanoHER2-SpyTag003 (GenBank and Addgene deposition in progress), based on the 11A4 nanobody against HER2 (Verheije et al. ...
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bioRxiv - Bioengineering 2024Quote: ... and Pert for which the hinge region was omitted (GenBank and Addgene deposition in progress). Each Fab light chain had the final assembly ...
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bioRxiv - Bioengineering 2024Quote: ... DoubleCatcher γ-Lock (GenBank and Addgene deposition in progress), DoubleCatcher δ-Lock (GenBank and Addgene deposition in progress) ...
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bioRxiv - Bioengineering 2024Quote: ... and pET28a-SnoopTag-SpyTag-(AffiHER2)3 (GenBank accession no. KU296976) (Addgene deposition in progress) (Veggiani et al. ...
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bioRxiv - Bioengineering 2024Quote: ... pDEST14-SpyCatcher003 S49C (Addgene plasmid ID 133448) (Vester et al. ...
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bioRxiv - Bioengineering 2024Quote: ... DoubleCatcher β-Lock (GenBank and Addgene deposition in progress), DoubleCatcher γ-Lock (GenBank and Addgene deposition in progress) ...
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bioRxiv - Bioengineering 2024Quote: ... and this construct was termed DoubleCatcher H-Lock (GenBank and Addgene deposition in progress). Double-cysteine substitution variants of DoubleCatcher were derived from pDEST14-SpyCatcher003-(GSG)3-SpyCatcher003-TEVs-SpyTag003DA by Gibson assembly ...
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bioRxiv - Bioengineering 2024Quote: ... pcDNA3.1-Tras heavy chain-SpyTag003 (‘Tras NoLink’) and pcDNA3.1-Tras light chain (GenBank and Addgene deposition in progress) were cloned previously by Jamie Lam (University of Oxford ...
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bioRxiv - Bioengineering 2024Quote: ... pET28a-SUMO-SpyTag003 (N-terminal His6 tag-SUMO protein-SpyTag003) was cloned previously by Irsyad Khairil Anuar (University of Oxford) (GenBank and Addgene deposition in progress). pDEST14-SpyCatcher003-TEVs-SpyTag003DA (‘Masked SpyCatcher0003’ ...
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bioRxiv - Bioengineering 2024Quote: ... Double-cysteine substitution variants of DoubleCatcher were derived from pDEST14-SpyCatcher003-(GSG)3-SpyCatcher003-TEVs-SpyTag003DA by Gibson assembly: DoubleCatcher α-Lock (GenBank and Addgene deposition in progress), DoubleCatcher β-Lock (GenBank and Addgene deposition in progress) ...
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bioRxiv - Bioengineering 2024Quote: ... pET28-SpyTag003-mClover3 (Addgene plasmid ID 133453) (Keeble et al. ...
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bioRxiv - Bioengineering 2024Quote: ... DoubleCatcher δ-Lock (GenBank and Addgene deposition in progress), and DoubleCatcher ε-Lock (GenBank and Addgene deposition in progress) ...
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bioRxiv - Bioengineering 2024Quote: ... was derived from pDEST14-SpyCatcher003 (GenBank Accession no. MN433887, Addgene plasmid ID 133447) by incorporating the TEV protease cleavage site (ENLYFQ/G ...
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bioRxiv - Bioengineering 2024Quote: ... pDEST14-SpyCatcher003-TEVs-SpyTag003DA (‘Masked SpyCatcher0003’; N-terminal His6 tag-SpyCatcher003-TEV protease cleavage site-SpyTag003 D117A, GenBank and Addgene deposition in progress) was derived from pDEST14-SpyCatcher003 (GenBank Accession no ...
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bioRxiv - Bioengineering 2024Quote: ... was a gift from Mark Arbing (Addgene plasmid # 179694). The histidine tag of the MBP-BirA plasmid was deleted using SLIC (39) ...
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bioRxiv - Developmental Biology 2024Quote: The original PA-Cre construct driven by the CMV promoter was purchased from Addgene (Addgene no. 122960). The PA-Cre coding sequence was synthesized by GenScript and listed in the supplementary note ...
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bioRxiv - Developmental Biology 2024Quote: The original PA-Cre construct driven by the CMV promoter was purchased from Addgene (Addgene no. 122960). The PA-Cre coding sequence was synthesized by GenScript and listed in the supplementary note ...
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bioRxiv - Cell Biology 2024Quote: ... mCherry-Rab5Q79L (Addgene #35138 ...
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bioRxiv - Cell Biology 2024Quote: ... and pCMV-VsVg (Addgene, MA, USA, #8454) into LentiX-293T cells to generate virus ...
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bioRxiv - Cell Biology 2024Quote: ... Eps15-GFP-His (Addgene #170860 ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-Rabenosyn5 (Addgene #37538 ...