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Citations for Addgene :
1501 - 1550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and then cloned into the EagI and EcoRI sites of pJK03 (AddGene #173,490) in multiple ligation reactions (NEB T4 ligase) ...
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bioRxiv - Genomics 2024Quote: ... Basal promoter inserts were prepared by amplifying the Rho basal promoter and DsRed from the plasmid pJK01 (AddGene #173,489) using the forward primer MO566 and reverse primers that add 9bp multiplexing barcodes (mBC ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse vsv-plexin-B3 and human pECE-M2-BAIAP2 (IRSP53-Flag) were purchased from Addgene (#68038, #68039, #31656, USA).
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Vectors used are listed in Key Resources Table (Addgene #99378 ...
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bioRxiv - Neuroscience 2024Quote: ... The following AAV vectors were purchased from Addgene: AAV5-hSyn-DIO-hM3D(Gq)-mCherry (#44361) ...
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bioRxiv - Neuroscience 2024Quote: ... Olig001 was a gift from Thomas McCown (Addgene plasmid # 170716; http://n2t.net/addgene:170716; RRID:Addgene_170716)79.
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bioRxiv - Neuroscience 2024Quote: ... pAAV.CAG.GCaMP6f.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100836-AAV1 ...
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bioRxiv - Neuroscience 2024Quote: ... pAAV.CAG.GCaMP6f.WPRE.SV40 was a gift from Douglas Kim & GENIE Project (Addgene viral prep # 100836-AAV1; http://n2t.net/addgene:100836; RRID:Addgene_100836)78 ...
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bioRxiv - Neuroscience 2024Quote: ... PV-Cre mice (N = 5) were injected at the same coordinates with 250 nL of AAV-ef1a-Flex-iChloC-2A-dsRed (Addgene plasmid ...
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bioRxiv - Neuroscience 2024Quote: ... a Cre-dependent AAV expressing GCaMP6f under either the CAG promoter (AAV5-CAG-DIO-GCaMP6f, Addgene, diluted to 3.5 × 1012 genome copies/mL ...
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bioRxiv - Cell Biology 2024Quote: ... The mGFP (Emerald) was amplified from Tom20-Emerald (#54282, Addgene, USA) and the CIBN was amplified from CIBN-CAAX (#79574 ...
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bioRxiv - Cell Biology 2024Quote: ... The PASSst sensor was created by fusion of two copies of PABD with the GCN4 that was amplified from pcDNA4TO-24xGCN4_v4-kif18b (#74934, Addgene, USA). The GCN4 probe was pHR-scFv-GCN4-sfGFP-GB1-dWPRE (#60907 ...
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bioRxiv - Cell Biology 2024Quote: ... mCherry-Rab5Q79L (Addgene #35138 ...
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bioRxiv - Cell Biology 2024Quote: ... and pCMV-VsVg (Addgene, MA, USA, #8454) into LentiX-293T cells to generate virus ...
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bioRxiv - Cell Biology 2024Quote: ... Eps15-GFP-His (Addgene #170860 ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-Rabenosyn5 (Addgene #37538 ...
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bioRxiv - Cancer Biology 2024Quote: ... and pMD2.G (gift from Didier Trono; Addgene plasmid # 12259) using Lipofectamine 3000 Transfection Reagent (Invitrogen) ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293T cells were transfected with the viral transfer plasmid lentiCas9-Blast (Sanjana et al., 2014) (gift from Feng Zhang; Addgene plasmid # 52962), the viral packaging plasmid psPAX2 (gift from Didier Trono ...
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bioRxiv - Cell Biology 2024Quote: ... MEFs and MDA-MB-231 cells were stably modified using BFP and DN-KASH expression plasmids in a doxycycline-inducible Piggybac plasmid backbone (Addgene #187019). For lentiviral modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... lentiGuide-PURO plasmid (gift from Feng Zhang; Addgene plasmid # 52963) via Gibson Assembly (New England BioLabs) ...
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bioRxiv - Cancer Biology 2024Quote: ... the viral packaging plasmid psPAX2 (gift from Didier Trono; Addgene plasmid # 12260), and the viral envelope plasmid pMD2.G (gift from Didier Trono ...
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bioRxiv - Cancer Biology 2024Quote: ... the Human GeCKOv2 CRISPR Knockout Pooled Library (A+B) in the lentiGuide-Puro vector backbone (gift from Feng Zhang; Addgene Plasmid # 1000000048) was amplified and purified as directed by Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... and the viral envelope plasmid pMD2.G (gift from Didier Trono; Addgene plasmid # 12259) using Lipofectamine 3000 Transfection Reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... fused with bGHpA amplified from LSL-Cas9-Rosa26TV (Addgene plasmid # 61408; http://n2t.net/addgene:61408; RRID:Addgene_61408; a gift from Feng Zhang) and Pac I/Sma I digested LSL-Cas9-Rosa26TV by In-Fusion HD ...
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bioRxiv - Cell Biology 2024Quote: ... The CMV promoter of mEmerald-C1 (Addgene plasmid # 53975 ...
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bioRxiv - Cell Biology 2024Quote: ... and psPAX2 (Addgene plasmid #12260; http://n2t.net/addgene:12260; RRID:Addgene_12260; a gift from Didier Trono), together with a pCDH vector (pCDH-NLSSV40-sfCherry-NLSMyc-Blast ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immortalized by transduction with a retrovirus expressing SV40 large T antigen from pBABE-puro largeTcDNA (Addgene plasmid # 14088 ...
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bioRxiv - Neuroscience 2024Quote: ... The he1.1:YFP cassette (he1.1:YFP_F, he1.1:YFP_R, Table 4) was amplified from p3E_he1a:YFP (Addgene, plasmid #113879), and the polyA terminator (polyA_F ...
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bioRxiv - Neuroscience 2024Quote: ... polyA_R, Table 3) and plasmid backbone (Col1E_F, Col1E_R, Table 4) were amplified from pPRISM-Stop-cmlc2-eGFP (Addgene kit #1000000154; Wierson et al., 2020). The PCR-amplified fragments were assembled using NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Neuroscience 2024Quote: ... Gbait-hsp70-QF2-pA (Addgene plasmid #122563), which contains a GFP bait sequence for Cas9-mediated linearization of the donor plasmid (Kimura et al. ...
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bioRxiv - Immunology 2024Quote: ... Three sgRNA constructs targeting STX4 were generated using the sgOpti plasmid (Addgene ID 85681) as a backbone and packaged into lentiviral particles58 ...
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bioRxiv - Cell Biology 2024Quote: ... and cofilin-1-EGFP (cat no. 50859) were purchased from Addgene (Watertown, MA).
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bioRxiv - Cancer Biology 2024Quote: ... the pCX-EGFP beta5 integrin receptor 20 was a gift from Raymond Birge (Addgene plasmid # 14996 ...
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bioRxiv - Cancer Biology 2024Quote: ... subcloning and sequencing The pLJM1-empty (#91980) and PD-1-miSFIT-1x (#124679) vectors were purchased from Addgene. PTEN and PD-1 were subcloned into linearized pLJM1-empty vector using EcoR1 and NHE1 restriction enzymes (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... Scramble lentiCRISPRv2 construct (Addgene, #169795) was used as a control sgRNA ...
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bioRxiv - Immunology 2024Quote: ... The pCI AscI MR1 R9H res construct (Addgene ID 214753) was based on work by Howson et al.24 ...
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bioRxiv - Immunology 2024Quote: ... as were psPAX2 (Addgene #12260) and pMD2.G (Addgene #12259) ...
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bioRxiv - Immunology 2024Quote: ... The pCW-Cas9 plasmid was obtained from Addgene (#50661), as were psPAX2 (Addgene #12260 ...
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bioRxiv - Immunology 2024Quote: ... pCI AscI MR1 res (Addgene ID 214752) and pCI AscI MR1 R9H res (Addgene ID 214753 ...
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bioRxiv - Immunology 2024Quote: ... were carried out as following: MaSCs (passage 2-3) were transduced with sgP53 or sgNT cloned into lentiCRISPRv2-puro (Addgene, Plasmid #98290), passaged once ...
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bioRxiv - Immunology 2024Quote: ... a gift from Didier Trono (Addgene, plasmids #12259 and #22036). Viruses were concentrated with the Lenti-X Concentrator reagent (Clontech ...
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bioRxiv - Cell Biology 2024Quote: ... and pAdDeltaF6 (Addgene plasmid #112867; http://n2t.net/addgene:112867; RRID: Addgene_112867) were a gift from James M ...
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bioRxiv - Cell Biology 2024Quote: ... or 2.0 µg noted IQGAP1 and GFP-actin (Addgene: 31502)93 plasmids for 24 h ...
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bioRxiv - Cell Biology 2024Quote: ... and pAdDeltaF6 (Addgene plasmid #112867 ...
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bioRxiv - Cell Biology 2024Quote: ... and psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Cell Biology 2024Quote: ... mycBioID2-pBABE-puro was a gift from Kyle Roux (Addgene plasmid #80900; http://n2t.net/addgene:80900; RRID: Addgene_80900).
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bioRxiv - Cell Biology 2024Quote: ... The pX459-sfCherry CRISPR-Cas9 vector were generated from pSpCas9(BB)-2A-Puro (pX459) V2.0 (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988; a gift from Feng Zhang). The annealed oligonucleotides to express single-guide RNA (sgRNA ...
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bioRxiv - Cell Biology 2024Quote: ... The CMV promoter of mEmerald-C1 (Addgene plasmid # 53975; http://n2t.net/addgene:53975; RRID:Addgene_53975; a gift from Michael Davidson) was replaced by the Lmna promoter using the In-Fusion HD Cloning Kit (Clontech ...
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bioRxiv - Cell Biology 2024Quote: ... mycBioID2-pBABE-puro was a gift from Kyle Roux (Addgene plasmid #80900 ...
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bioRxiv - Cell Biology 2024Quote: ... The pX459-sfCherry CRISPR-Cas9 vector were generated from pSpCas9(BB)-2A-Puro (pX459) V2.0 (Addgene plasmid # 62988 ...