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Citations for Addgene :
8451 - 8500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... SMASh (Addgene 68853), and FKBP12 (Addgene 17416 ...
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bioRxiv - Bioengineering 2023Quote: ... and OsTIR1 (Addgene 72834) auxin receptor F-box proteins ...
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bioRxiv - Bioengineering 2023Quote: ... The AQP1-miniIAA7 and AQP1-AID expressing cell lines were transduced a second time with lentivirus encoding AtAFB2 (Addgene 129718) and OsTIR1 (Addgene 72834 ...
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bioRxiv - Bioengineering 2023Quote: ... and FKBP12 (Addgene 17416) were amplified using Q5 High-Fidelity 2X Master Mix and cloned by Gibson assembly in a lentiviral transfer vector at the C or N-terminus of the aquaporin-1 reporter (Aqp1 ...
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bioRxiv - Bioengineering 2023Quote: Plasmids harboring the various degron sequences - DHFR (Addgene 29326), ER (Addgene 37261) ...
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bioRxiv - Bioengineering 2023Quote: ... miniIAA7 (Addgene 129721), SMASh (Addgene 68853) ...
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bioRxiv - Bioengineering 2023Quote: ... EF1α (Addgene 60058) or a doxycycline-inducible minimal CMV promoter (Addgene 26431) ...
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bioRxiv - Bioengineering 2023Quote: ... Each well was transfected with 7.5 μg of one sgRNA plasmid of interest and 7.5 μg of a SpCas9 encoding plasmid (AddGene plasmid# 48137) through the calcium phosphate precipitation method as previously described (31) ...
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bioRxiv - Bioengineering 2023Quote: ... and a plasmid containing both an empty sgRNA scaffold as well as a gene sequence encoding SpCas9 (AddGene plasmid# 57819) with a final goal of LV positive control vector generation ...
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bioRxiv - Bioengineering 2023Quote: ... Two plasmids were chosen for further development: a plasmid containing both an empty sgRNA scaffold as well as GFP (AddGene plasmid# 99375) with a final goal of VLP generation ...
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bioRxiv - Bioengineering 2023Quote: ... pET-28a (+) vector was obtained as a gift from Hermann Gaub (Addgene plasmid # 90473; http://n2t.net/addgene:90473; RRID: Addgene_90473). The PCR production and pET-28a (+ ...
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bioRxiv - Biochemistry 2023Quote: ... KpSduA and PaSduA were inserted into the 8A (Addgene: 37501) plasmid using ligation-independent cloning (LIC) ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmids pCytERM_mScarlet_N1 (Addgene Plasmid # 85066), pC1-HyPer-Red (Addgene Plasmid # 48249) ...
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bioRxiv - Biochemistry 2023Quote: ... stably expressing Dox-inducible shControl and shBRAT1 were made by tet-pLKO-puro vectors purchased from Addgene (Cat# 21915). Dox (1 ug/ml ...
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bioRxiv - Biochemistry 2023Quote: ... with the only difference being the use of the pAAV2/8 packing plasmid (Addgene Plasmid #112864) for serotyping ...
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bioRxiv - Biochemistry 2023Quote: ... we ligated the gel-purified fragments at the HindIII and BamH1 site and inserted into the HindIII and BamH1 digested linear pT7-CMVtrans-FFLuc-polyA vector (Addgene 101156). We confirmed positive clones for the full length of the insert by DNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: ... the strain was transformed with the TRP1-GAL1 cassette from pFA6-TRP1-PGAL1 (RRID:Addgene_41606, ref. (76)) ...
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bioRxiv - Biochemistry 2023Quote: ... the plasmid pFA6-His3MX6-PGAL1-3HA (RRID:Addgene_41610, ref. (76)) was used to obtain the His3MX6-PGAL1-3HA cassette by PCR amplification (see Additional file 6 ...
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bioRxiv - Biophysics 2023Quote: ... and envelope plasmid VSV-G (0.6 µg; Addgene #12259) were mixed with reduced-serum Minimal Essential Medium (Opti-MEM ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA for expressing CRY2olig tagged with mCherry at its C-terminus was obtained from Addgene (#60032; Watertown, MA, USA). The plasmid (1.0 µg/dish ...
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bioRxiv - Biophysics 2023Quote: ... The plasmid was given by the Michael Rosen group (Addgene plasmid #127093).
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bioRxiv - Bioengineering 2023Quote: The ST7 expression plasmid was synthesized by using the public sequence of the pX330-U6-Chimeric_BB-CBh-hSpCas9 plasmid (Addgene #42230)(Cong et al. ...
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bioRxiv - Biochemistry 2023Quote: ... and S545A (HS-BG) constructs were cloned into a pHIV-dTomato lentiviral backbone (Plasmid #21374, Addgene, Cambridge, MA) by the Center for Targeted Therapeutics Core Facility at the University of South Carolina (Columbia ...
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bioRxiv - Biophysics 2023Quote: The full-length α-synuclein monomer (1−140) expression plasmid (pET21a-alpha-synuclein) was a gift from Michael J Fox Foundation MJFF (Addgene plasmid # 51486). The α-synuclein monomer was expressed and purified following the previous method20,21 ...
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bioRxiv - Biochemistry 2023Quote: ... pcDNA3.1-hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid #135411 ...
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bioRxiv - Biochemistry 2023Quote: ... pME-NR2F2 was a gift from Nathan Lawson (Addgene plasmid #138359 ...
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bioRxiv - Biochemistry 2023Quote: ... pcDNA3.1-hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid #135411; http://n2t.net/addgene:135411; RRID:Addgene_135411). pIRE4-Azi was a kind gift from Irene Coin (Addgene plasmid #105829 ...
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bioRxiv - Biochemistry 2023Quote: ... pRSV-Rev (Addgene plasmid #12253 ...
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bioRxiv - Biochemistry 2023Quote: ... pcDNA Myc DBC1 was a gift from Osamu Hiraike (Addgene plasmid # 35096 http://n2t.net/addgene:35096; RRID:Addgene_35096). pcDNA3.1-hRARα.hRXRα was a gift from Catharine Ross (Addgene plasmid #135411 ...
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bioRxiv - Biochemistry 2023Quote: ... and pMDLg/pRRE (Addgene plasmid #12251; http://n2t.net/addgene:12251; RRID:Addgene 12251)(35) ...
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bioRxiv - Biochemistry 2023Quote: ... pRSV-Rev (Addgene plasmid #12253; http://n2t.net/addgene:12253; RRID:Addgene 12253)(35) ...
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bioRxiv - Biochemistry 2023Quote: ... Lentiviruses with this construct were produced by transfecting human embryonic kidney 293T (HEK 293T) cells with pKS18 and the lentiviral packaging vectors pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Biochemistry 2023Quote: ... and pMDLg/pRRE (Addgene plasmid #12251 ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Biochemistry 2023Quote: ... For the expression of CFP (e.g. Addgene #173083) and YFP tagged recombinant proteins (Addgene #173080) ...
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bioRxiv - Bioengineering 2023Quote: ... We ordered pairs of complementary forward and reverse single stranded oligonucleotides (IDT) for each guide containing compatible overhang sites for insertion into a lentiCRISPRv2-Puro (Addgene #98290) backbone ...
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bioRxiv - Bioengineering 2023Quote: ... A plasmid encoding pDisplay-mSA (Addgene #39863) was generously provided by Dr ...
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bioRxiv - Bioengineering 2023Quote: ... Fluorescent expressing C2C12s were created by introducing copGFP (pCDH-EF1-copGFP-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 72263; http://n2t.net/addgene:72263; RRID:Addgene_72263) and fluorescent HMEC1s were created by introducing mCherry (pCDH-CMV-mCherry-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 72264 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and subcloned into both pHemmarG (Addgene #31221) for CD4:tdGFP reporters ...
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bioRxiv - Cell Biology 2023Quote: ... was cloned into pLKO-Tet-On (Addgene #21915).
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bioRxiv - Evolutionary Biology 2023Quote: ... and pBPGUw (Addgene #17575) for Gal4 drivers ...
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bioRxiv - Genetics 2023Quote: ... or Lenti-guide blast (a gift from Brett Stringer (Addgene plasmid #104993; http://n2t.net/addgene:104993; RRID:Addgene_104993)) plasmids (102 ...
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bioRxiv - Genetics 2023Quote: ... were cloned into a Lenti-guide puro (a gift from Feng Zhang (Addgene plasmids #52963 ...
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bioRxiv - Genetics 2023Quote: ... or Lenti-guide blast (a gift from Brett Stringer (Addgene plasmid #104993 ...
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bioRxiv - Genetics 2023Quote: ... We cloned oligonucleotides encoding the gRNA spacers into BPK1520 (Addgene plasmid # 65777) to generate gRNA expression plasmids ...
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bioRxiv - Genetics 2023Quote: ... pMD2.G and psPAX2 were a gift from Didier Trono (Addgene plasmids #12259 and 12260 ...
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bioRxiv - Genetics 2023Quote: ... 1.2 μg of pMD2.G (Addgene 12259) and 12 μl of PLUS reagent were added to 3 ml of OPTI-MEM and incubated for 5 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... We first integrated homology arms into the base donor plasmid pSL1180-HR-PUbECFP (Addgene #47917) (MCMENIMAN et al ...
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bioRxiv - Genetics 2023Quote: ... was generated by removing the improved gRNA scaffold from pKLV2 - U6 - gRNA5(BbsI) - PGKpuro - mCherry - W (Addgene 67977) (7 with minor changes) ...
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bioRxiv - Genetics 2023Quote: ... or the SpG variant capable of targeting sites encoding NGA PAMs (RTW4177, Addgene plasmid # 139988) (Supplementary table 2) ...