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Citations for Addgene :
8651 - 8700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... a gift from Michael Davidson (Addgene plasmid #58023).
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bioRxiv - Neuroscience 2023Quote: pCS2(xtFapb7):mTagBFP2-Ras: BFP from pBAD-mTagBFP2 (Addgene plasmid # 34632 ; http://n2t.net/addgene:34632 ; RRID:Addgene_34632) (Subach et al. ...
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bioRxiv - Neuroscience 2023Quote: ... the Egfp was first amplified from pCS2:mito-EGFP-mCherry (Davis et al., 2014) using CD53 and CD91h and cloned into pTRETight:MitoTimer (Addgene plasmid # 50547 ...
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bioRxiv - Genomics 2023Quote: ... The original CRISPRoff plasmid DNA (except the region encoding TagBFP) and mScarletI cDNA from the pmScarlet-i_C1 plasmid (Addgene # 85044) were amplified by polymerase chain reaction (PCR ...
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bioRxiv - Genomics 2023Quote: ... whose construction by APPEAL will be described separately) used in the study originated from Addgene-deposited plasmids including lentiCas9-Blast (#52962) ...
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bioRxiv - Neuroscience 2023Quote: ... AAVretro-CAG-FLPo and AAV-CAG-FRT-rev-TdTomato were gifts from Janelia Viral Tools (Addgene plasmid #183412 ...
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bioRxiv - Molecular Biology 2023Quote: ... containing the AAVS1-T2 targeting guide and pSH-EFIRES-P-AtAFB2 (#129715, Addgene), which was digested using BglII [#R0144 ...
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bioRxiv - Molecular Biology 2023Quote: ... the corresponding oligonucleotides (Table S1) were annealed and cloned in the pU6-BbsI-chiRNA vector linearized with BbsI (Addgene plasmid #45946 ...
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bioRxiv - Molecular Biology 2023Quote: ... The pMH4_HA plasmid was constructed on the basis of the pMH4 vector (Addgene plasmid #52529 ...
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bioRxiv - Neuroscience 2023Quote: ... and e37b-Cacna1b (Addgene: 26571) splice isoforms ...
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bioRxiv - Neuroscience 2023Quote: ... We expressed mammalian cDNAs encoding calcium-channel e37a-Cacna1b (Addgene: 26569) and e37b-Cacna1b (Addgene ...
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bioRxiv - Neuroscience 2023Quote: ... or AAV-hSyn-DIO-hM3D(Gq)-mCherry (Addgene, 44361-AAV8) were injected at a titer of 4.0 x 1012 GC/ml.
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bioRxiv - Neuroscience 2023Quote: ... or AAV-hSyn-Cre-P2A-dTomato (Addgene, 107738-AAV5) were injected at a titer of 3.0 x 1011 GC/ml ...
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bioRxiv - Neuroscience 2023Quote: ... either AAV-hSyn-mCherry (Addgene, 114472-AAV5) or AAV-hSyn-Cre-P2A-dTomato (Addgene ...
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bioRxiv - Molecular Biology 2023Quote: The OSC cells were transfected with the pAc-sgRNA-Cas9Flag plasmid (Addgene plasmid #49330 ...
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bioRxiv - Molecular Biology 2023Quote: ... fused to a nuclear localization signal (NLS) and RNA guide was a gift from Jennifer Doudna (Addgene plasmid #195240; http://n2t.net/addgene:195240; RRID: Addgene_195240) (16) ...
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bioRxiv - Molecular Biology 2023Quote: pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #186981 ...
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bioRxiv - Molecular Biology 2023Quote: pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid #48139 ...
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bioRxiv - Molecular Biology 2023Quote: pDF0159 pCMV - huDisCas7-11 mammalian expression plasmid was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #172507; http://n2t.net/addgene:172507; RRID:Addgene_172507) (18) ...
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bioRxiv - Molecular Biology 2023Quote: pDF0114 pu6-eco31i-eco31i-dis7-11-mature-dr-guide-scaffold was a gift from Omar Abudayyeh & Jonathan Gootenberg (Addgene plasmid #186981; http://n2t.net/addgene:186981 ; RRID: Addgene_186981) (29) ...
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bioRxiv - Molecular Biology 2023Quote: pSpCas9(BB)-2A-Puro (PX459) was a gift from Feng Zhang (Addgene plasmid #48139; http://n2t.net/addgene:48139;RRID:Addgene_48139). Cas9 sgRNAs targeting the RTCB gene were cloned as previously described (table S2 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Cas6) fused to a nuclear localization signal (NLS) and RNA guide was a gift from Jennifer Doudna (Addgene plasmid #195240 ...
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bioRxiv - Neuroscience 2023Quote: ... and pZac2.1 gfaABC1D-lck-GCaMP6f virus (Addgene virus #52924).
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bioRxiv - Neuroscience 2023Quote: ... commercially available viruses were used: pAAV-GFAP-hM3D(Gq)-mCherry (Addgene virus #50478) and pZac2.1 gfaABC1D-lck-GCaMP6f virus (Addgene virus #52924).
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bioRxiv - Neuroscience 2023Quote: ... pCMV-p35 (gift from Dr. Li-Huei Tsai, Addgene plasmid # 1347, Watertown, MA), was transfected into Neuro 2a cells using Neuro-2a Cell Avalanchetransfection reagent (EZ Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmids used in this study are modified from the pSox2-bd::FP construct (Addgene plasmid #34703; Bestman et al., 2015), a plasmid that contains a weak FGF4 promotor regulated by 6 repeats of the Sox2 transcription factor binding domain ...
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bioRxiv - Neuroscience 2023Quote: ... AAVretro-CAG-FLPo and AAV-CAG-FRT-rev-TdTomato were gifts from Janelia Viral Tools (Addgene plasmid #183412, http://n2t.net/addgene:183412, RRID:Addgene_183412; Addgene plasmid #191203 ...
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bioRxiv - Neuroscience 2023Quote: ... AAVretro-CAG-FLPo and AAV-CAG-FRT-rev-TdTomato were gifts from Janelia Viral Tools (Addgene plasmid #183412, http://n2t.net/addgene:183412, RRID:Addgene_183412; Addgene plasmid #191203, http://n2t.net/addgene:191203, RRID:Addgene_191203). The titers of each virus were as follows (in genomic copies/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... and Feng Zhang (Addgene plasmid #42230; [73]), respectively ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids p2lox and pX330-U6-Chimeric_BB-CBh-hSpCas9 were kindly provided by Michael Kyba (Addgene plasmid #34635; [46]) and Feng Zhang (Addgene plasmid #42230 ...
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bioRxiv - Molecular Biology 2023Quote: ... (Addgene Plasmid #83482 ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293FT cells were co-transfected with the Tet-on ssRFP-GFP-KDEL plasmid (Addgene 128257, Noboru Mizushima) and the lentivirus packaging plasmids psPAX2 (Addgene 12260 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the lentivirus packaging plasmids psPAX2 (Addgene 12260, Didier Trono) and pMD2.G (Addgene 12259 ...
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bioRxiv - Molecular Biology 2023Quote: ... The guide RNAs sequences were inserted into the pGL3-U6-sgRNA-PGK-puromycin plasmid (Addgene 51133, Xingxu Huang). U-2 OS ...
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bioRxiv - Molecular Biology 2023Quote: ... HeLa or HEK293FT cells were co-transfected with the sgRNA plasmid and the pST1374-NLS-flag-linker-Cas9 (Addgene 44758, Xingxu Huang). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... and pMD2.G (Addgene 12259, Didier Trono) using Lipofectamine® 2000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... a zebrafish codon optimized Cas9 (pT3TS-nCas9n, Addgene plasmid # 46757) was linearized with XbaI overnight and 1 μg of linear template DNA was used for in vitro transcription of mRNA using the T3 mMESSAGE mMACHINE® Kit (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: The Brainbow adeno-associated viruses (AAVs) (AAV-EF1a-BbTagBY [Addgene #45185-AAV9] and AAV-EF1a-BbChT [Addgene #45186-AAV9] ...
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bioRxiv - Neuroscience 2023Quote: ... pcDNA3 Rod Opsin plasmid (hRho) was obtained from Addgene (plasmid #109361). GloSensor-22F plasmid was obtained from Promega Corporation (E2301) ...
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bioRxiv - Neuroscience 2023Quote: All plasmids used in these studies are available from Addgene. For all primers referenced below ...
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bioRxiv - Neuroscience 2023Quote: ... with GFP1-9::iRFP702 (Addgene #130125), mouseStoml3-GFP10::mCHERRY and mouseElkin1-GFP11::eBP2 plasmids using Fugene HD and following manufacturer’s instructions (Promega) ...
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bioRxiv - Neuroscience 2023Quote: ... then inserted into an AgeI-/EcoRI-linearized pLenti-hSyn backbone (Addgene #86641) by Gibson assembly ...
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bioRxiv - Neuroscience 2023Quote: A volume of 200 nL of pGP-AAV2/5-syn-FLEX-jGCaMP8m-WPRE (1.9×1012 pp/mL, Addgene; #162378) expressing the genetically encoded calcium indicator GcaMP8m was injected into the CA2 region of the right hemisphere at AP -2.0 mm ...
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bioRxiv - Neuroscience 2023Quote: A glass capillary pulled to a fine tip was pre-loaded with a retrograde virus expressing both GFP and channelrhodopsin (ChR2) (pAAV-Syn-ChR2(H134R)-GFP (Addgene, 7 x 1012 vg/ml in PBS/NaCl/pluronic acid F-68 ...
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Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxietybioRxiv - Neuroscience 2023Quote: The following viruses were obtained from Addgene: for chemogenetics ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV1-FLEX-Arch-GFP (Cat. No. 22222) were purchased from Addgene. For all experiments ...
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bioRxiv - Neuroscience 2023Quote: ... bolus injections of either AAV serotype 9 or 2 were (Addgene, Watertown, MA, USA) delivered and followed by a flush of 200 μL of sterile saline ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... IP3R2-KO and CB f/f mice were injected with AAV5-pZac2.1-gfaABC1d-cyto-GCaMP6f (Addgene), AAV-5/2-hGFAP-hM3D(Gq)/hM4D(Gi)_mCherry-WPRE-hGHp(A ...
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bioRxiv - Neuroscience 2023Quote: Five monkeys received bilateral infusions of the DREADD viral vector AAV5-hSyn-HA-hM3Dq obtained from Addgene (Watertown ...
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bioRxiv - Neuroscience 2023Quote: ... 1.3×1012 vg/mL) was mixed with AAV1-CAG-FLEX-EGFP-WPRE (Addgene; Cat # 51502; ≥ 1×1013 vg/mL) in a 4:1 ratio ...